Enhanced singlet oxygen production by photodynamic therapy and a novel method for its intracellular measurement

Autores
Luengas, Sandra L. Pena; Marin, Gustavo Horacio; Aviles, Kevin; Acuña, Ricardo Cruz; Roque, Gustavo; Nieto, Felipe Rodríguez; Sánchez, Francisco Homero; Tarditi, Adrián; Rivera, Luis; Mansilla, Eduardo
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The generation of singlet oxygen (SO) in the presence of specific photosensitizers (PSs) or semiconductor quantum dots (QDs) and its application in photodynamic therapy (PDT) is of great interest to develop cancer therapies with no need of surgery, chemotherapy, and/or radiotherapy. This work was focused on the identification of the main factors leading to the enhancement of SO production using Rose Bengal (RB), and Methylene Blue (MB) as PS species in organic and aqueous mediums. Subsequently, the capacity of zinc oxide (ZnO), zinc sulfide (ZnS), and ZnO/ZnS core-shell QDs as well as manganese (Mn+2) doped ZnO and ZnS nanoparticles (NPs) as potential PS was also investigated. Many variable parameters such as type of quencher, PSs, NPs, as well as its different concentrations, light source, excitation wavelength, reaction time, distance from light source, and nature of solvent were used. The degradation kinetics of the quenchers generated by SO species and the corresponding quantum yields were determined by monitoring the photo-oxidation of the chemical quencher and measuring its disappearance by fluorometry and spectrophotometry in the presence of NPs. Small intracellular changes of SO induced by these metal Zn (zinc) NPs and PDT could execute and accelerate deadly programs in these leukemic cells, providing in this way an innovative modality of treatment. In order to perform further more specific in vitro cytotoxic studies on B-chronic lymphocytic leukemia cells exposed to Zn NPs and PDT, we needed first to measure and ascertain those possible intracellular SO variations generated by this type of treatment; for this purpose, we have also developed and tested a novel method first described by us.
Fil: Luengas, Sandra L. Pena. Universidad de Puerto Rico; Puerto Rico
Fil: Marin, Gustavo Horacio. Cucaiba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Aviles, Kevin. Universidad de Puerto Rico; Puerto Rico
Fil: Acuña, Ricardo Cruz. Universidad de Puerto Rico; Puerto Rico
Fil: Roque, Gustavo. Cucaiba; Argentina
Fil: Nieto, Felipe Rodríguez. Universidad Nacional Arturo Jauretche; Argentina
Fil: Sánchez, Francisco Homero. Universidad Nacional de La Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tarditi, Adrián. Cucaiba; Argentina
Fil: Rivera, Luis. Universidad de Puerto Rico; Puerto Rico
Fil: Mansilla, Eduardo. Cucaiba; Argentina
Materia
B-Cll
Chemotherapy
Lymphoma
Targeted Therapy
Treatment
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/37144
Acceder
id CONICETDig_0e03f22284244dd5f9f6694c10648874
oai_identifier_str oai:ri.conicet.gov.ar:11336/37144
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Enhanced singlet oxygen production by photodynamic therapy and a novel method for its intracellular measurementLuengas, Sandra L. PenaMarin, Gustavo HoracioAviles, KevinAcuña, Ricardo CruzRoque, GustavoNieto, Felipe RodríguezSánchez, Francisco HomeroTarditi, AdriánRivera, LuisMansilla, EduardoB-CllChemotherapyLymphomaTargeted TherapyTreatmenthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The generation of singlet oxygen (SO) in the presence of specific photosensitizers (PSs) or semiconductor quantum dots (QDs) and its application in photodynamic therapy (PDT) is of great interest to develop cancer therapies with no need of surgery, chemotherapy, and/or radiotherapy. This work was focused on the identification of the main factors leading to the enhancement of SO production using Rose Bengal (RB), and Methylene Blue (MB) as PS species in organic and aqueous mediums. Subsequently, the capacity of zinc oxide (ZnO), zinc sulfide (ZnS), and ZnO/ZnS core-shell QDs as well as manganese (Mn+2) doped ZnO and ZnS nanoparticles (NPs) as potential PS was also investigated. Many variable parameters such as type of quencher, PSs, NPs, as well as its different concentrations, light source, excitation wavelength, reaction time, distance from light source, and nature of solvent were used. The degradation kinetics of the quenchers generated by SO species and the corresponding quantum yields were determined by monitoring the photo-oxidation of the chemical quencher and measuring its disappearance by fluorometry and spectrophotometry in the presence of NPs. Small intracellular changes of SO induced by these metal Zn (zinc) NPs and PDT could execute and accelerate deadly programs in these leukemic cells, providing in this way an innovative modality of treatment. In order to perform further more specific in vitro cytotoxic studies on B-chronic lymphocytic leukemia cells exposed to Zn NPs and PDT, we needed first to measure and ascertain those possible intracellular SO variations generated by this type of treatment; for this purpose, we have also developed and tested a novel method first described by us.Fil: Luengas, Sandra L. Pena. Universidad de Puerto Rico; Puerto RicoFil: Marin, Gustavo Horacio. Cucaiba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Aviles, Kevin. Universidad de Puerto Rico; Puerto RicoFil: Acuña, Ricardo Cruz. Universidad de Puerto Rico; Puerto RicoFil: Roque, Gustavo. Cucaiba; ArgentinaFil: Nieto, Felipe Rodríguez. Universidad Nacional Arturo Jauretche; ArgentinaFil: Sánchez, Francisco Homero. Universidad Nacional de La Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tarditi, Adrián. Cucaiba; ArgentinaFil: Rivera, Luis. Universidad de Puerto Rico; Puerto RicoFil: Mansilla, Eduardo. Cucaiba; ArgentinaMary Ann Liebert2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37144Luengas, Sandra L. Pena; Marin, Gustavo Horacio; Aviles, Kevin; Acuña, Ricardo Cruz; Roque, Gustavo; et al.; Enhanced singlet oxygen production by photodynamic therapy and a novel method for its intracellular measurement; Mary Ann Liebert; Cancer Biotherapy And Radiopharmaceuticals; 29; 10; 12-2014; 435-4431084-9785CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1089/cbr.2014.1718info:eu-repo/semantics/altIdentifier/url/http://online.liebertpub.com/doi/abs/10.1089/cbr.2014.1718info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:46Zoai:ri.conicet.gov.ar:11336/37144instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:46.775CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Enhanced singlet oxygen production by photodynamic therapy and a novel method for its intracellular measurement
title Enhanced singlet oxygen production by photodynamic therapy and a novel method for its intracellular measurement
spellingShingle Enhanced singlet oxygen production by photodynamic therapy and a novel method for its intracellular measurement
Luengas, Sandra L. Pena
B-Cll
Chemotherapy
Lymphoma
Targeted Therapy
Treatment
title_short Enhanced singlet oxygen production by photodynamic therapy and a novel method for its intracellular measurement
title_full Enhanced singlet oxygen production by photodynamic therapy and a novel method for its intracellular measurement
title_fullStr Enhanced singlet oxygen production by photodynamic therapy and a novel method for its intracellular measurement
title_full_unstemmed Enhanced singlet oxygen production by photodynamic therapy and a novel method for its intracellular measurement
title_sort Enhanced singlet oxygen production by photodynamic therapy and a novel method for its intracellular measurement
dc.creator.none.fl_str_mv Luengas, Sandra L. Pena
Marin, Gustavo Horacio
Aviles, Kevin
Acuña, Ricardo Cruz
Roque, Gustavo
Nieto, Felipe Rodríguez
Sánchez, Francisco Homero
Tarditi, Adrián
Rivera, Luis
Mansilla, Eduardo
author Luengas, Sandra L. Pena
author_facet Luengas, Sandra L. Pena
Marin, Gustavo Horacio
Aviles, Kevin
Acuña, Ricardo Cruz
Roque, Gustavo
Nieto, Felipe Rodríguez
Sánchez, Francisco Homero
Tarditi, Adrián
Rivera, Luis
Mansilla, Eduardo
author_role author
author2 Marin, Gustavo Horacio
Aviles, Kevin
Acuña, Ricardo Cruz
Roque, Gustavo
Nieto, Felipe Rodríguez
Sánchez, Francisco Homero
Tarditi, Adrián
Rivera, Luis
Mansilla, Eduardo
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv B-Cll
Chemotherapy
Lymphoma
Targeted Therapy
Treatment
topic B-Cll
Chemotherapy
Lymphoma
Targeted Therapy
Treatment
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The generation of singlet oxygen (SO) in the presence of specific photosensitizers (PSs) or semiconductor quantum dots (QDs) and its application in photodynamic therapy (PDT) is of great interest to develop cancer therapies with no need of surgery, chemotherapy, and/or radiotherapy. This work was focused on the identification of the main factors leading to the enhancement of SO production using Rose Bengal (RB), and Methylene Blue (MB) as PS species in organic and aqueous mediums. Subsequently, the capacity of zinc oxide (ZnO), zinc sulfide (ZnS), and ZnO/ZnS core-shell QDs as well as manganese (Mn+2) doped ZnO and ZnS nanoparticles (NPs) as potential PS was also investigated. Many variable parameters such as type of quencher, PSs, NPs, as well as its different concentrations, light source, excitation wavelength, reaction time, distance from light source, and nature of solvent were used. The degradation kinetics of the quenchers generated by SO species and the corresponding quantum yields were determined by monitoring the photo-oxidation of the chemical quencher and measuring its disappearance by fluorometry and spectrophotometry in the presence of NPs. Small intracellular changes of SO induced by these metal Zn (zinc) NPs and PDT could execute and accelerate deadly programs in these leukemic cells, providing in this way an innovative modality of treatment. In order to perform further more specific in vitro cytotoxic studies on B-chronic lymphocytic leukemia cells exposed to Zn NPs and PDT, we needed first to measure and ascertain those possible intracellular SO variations generated by this type of treatment; for this purpose, we have also developed and tested a novel method first described by us.
Fil: Luengas, Sandra L. Pena. Universidad de Puerto Rico; Puerto Rico
Fil: Marin, Gustavo Horacio. Cucaiba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Aviles, Kevin. Universidad de Puerto Rico; Puerto Rico
Fil: Acuña, Ricardo Cruz. Universidad de Puerto Rico; Puerto Rico
Fil: Roque, Gustavo. Cucaiba; Argentina
Fil: Nieto, Felipe Rodríguez. Universidad Nacional Arturo Jauretche; Argentina
Fil: Sánchez, Francisco Homero. Universidad Nacional de La Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tarditi, Adrián. Cucaiba; Argentina
Fil: Rivera, Luis. Universidad de Puerto Rico; Puerto Rico
Fil: Mansilla, Eduardo. Cucaiba; Argentina
description The generation of singlet oxygen (SO) in the presence of specific photosensitizers (PSs) or semiconductor quantum dots (QDs) and its application in photodynamic therapy (PDT) is of great interest to develop cancer therapies with no need of surgery, chemotherapy, and/or radiotherapy. This work was focused on the identification of the main factors leading to the enhancement of SO production using Rose Bengal (RB), and Methylene Blue (MB) as PS species in organic and aqueous mediums. Subsequently, the capacity of zinc oxide (ZnO), zinc sulfide (ZnS), and ZnO/ZnS core-shell QDs as well as manganese (Mn+2) doped ZnO and ZnS nanoparticles (NPs) as potential PS was also investigated. Many variable parameters such as type of quencher, PSs, NPs, as well as its different concentrations, light source, excitation wavelength, reaction time, distance from light source, and nature of solvent were used. The degradation kinetics of the quenchers generated by SO species and the corresponding quantum yields were determined by monitoring the photo-oxidation of the chemical quencher and measuring its disappearance by fluorometry and spectrophotometry in the presence of NPs. Small intracellular changes of SO induced by these metal Zn (zinc) NPs and PDT could execute and accelerate deadly programs in these leukemic cells, providing in this way an innovative modality of treatment. In order to perform further more specific in vitro cytotoxic studies on B-chronic lymphocytic leukemia cells exposed to Zn NPs and PDT, we needed first to measure and ascertain those possible intracellular SO variations generated by this type of treatment; for this purpose, we have also developed and tested a novel method first described by us.
publishDate 2014
dc.date.none.fl_str_mv 2014-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/37144
Luengas, Sandra L. Pena; Marin, Gustavo Horacio; Aviles, Kevin; Acuña, Ricardo Cruz; Roque, Gustavo; et al.; Enhanced singlet oxygen production by photodynamic therapy and a novel method for its intracellular measurement; Mary Ann Liebert; Cancer Biotherapy And Radiopharmaceuticals; 29; 10; 12-2014; 435-443
1084-9785
CONICET Digital
CONICET
url http://hdl.handle.net/11336/37144
identifier_str_mv Luengas, Sandra L. Pena; Marin, Gustavo Horacio; Aviles, Kevin; Acuña, Ricardo Cruz; Roque, Gustavo; et al.; Enhanced singlet oxygen production by photodynamic therapy and a novel method for its intracellular measurement; Mary Ann Liebert; Cancer Biotherapy And Radiopharmaceuticals; 29; 10; 12-2014; 435-443
1084-9785
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1089/cbr.2014.1718
info:eu-repo/semantics/altIdentifier/url/http://online.liebertpub.com/doi/abs/10.1089/cbr.2014.1718
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert
publisher.none.fl_str_mv Mary Ann Liebert
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268880588767232
score 13.13397

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