Multimethodological approach to gastrointestinal microsporidiosis in HIV-infected patients

Autores
Velásquez, Jorge Néstor; Di Risio, Cecilia Diana; Etchart, Cristina; Chertcoff, Agustín Víctor; Astudillo, Osvaldo Germán; Carnevale, Silvana
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Purpose Microsporidiosis is an opportunistic infection that produces chronic diarrhoea and cholangiopathy in patients with AIDS, mainly caused by two species of microsporidia, Enterocytozoon bieneusi and Encephalitozon intestinalis. The aim of this work was to develop an integral system for the diagnosis of microsporidiosis of the intestine and biliary tract in HIVinfected patients, comprising microscopic and molecular techniques. Methods The study population comprised 143 adult patients of both sexes with diagnosis of HIV infection, with chronic diarrhoea, and with or without HIV-associated cholangiopathy. Stool studies for microsporidia identifcation of spores were performed on each patient. A video esofagogastroduodenoscopy with biopsy collection was also carried out for routine histology and semi-thin sections stained with Azure II. Species identifcation was carried out by transmission electron microscopy and/or polymerase chain reaction for the species E. bieneusi and E. intestinalis. Results Out of the 143 patients a total of 12.6% (n=18) were infected with microsporidia. Microsporidia species identifed in most cases was E. bieneusi (16/18 cases), followed by E. intestinalis (4/18), all of these last ones in coinfection with E. bieneusi. Conclusions Clinical, imaging, microscopic and molecular analyses, when applied in a systematic and integrated approach, allow diagnosis and identifcation of microsporidia at species level in AIDS patients with chronic diarrhoea, and with or without HIV-associated cholangiopathy.
Fil: Velásquez, Jorge Néstor. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina
Fil: Di Risio, Cecilia Diana. Hospital Municipal General de Agudos Doctor José Penna; Argentina
Fil: Etchart, Cristina. Hospital Municipal General de Agudos Doctor José Penna; Argentina
Fil: Chertcoff, Agustín Víctor. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Astudillo, Osvaldo Germán. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Carnevale, Silvana. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
ENCEPHALITOZOON INTESTINALIS
ENTEROCYTOZOON BIENEUSI
HIV
MICROSPORIDIOSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/122862
Acceder
id CONICETDig_0dd99fdb9f9a24630a21ee0a5cba7f66
oai_identifier_str oai:ri.conicet.gov.ar:11336/122862
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Multimethodological approach to gastrointestinal microsporidiosis in HIV-infected patientsVelásquez, Jorge NéstorDi Risio, Cecilia DianaEtchart, CristinaChertcoff, Agustín VíctorAstudillo, Osvaldo GermánCarnevale, SilvanaENCEPHALITOZOON INTESTINALISENTEROCYTOZOON BIENEUSIHIVMICROSPORIDIOSIShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Purpose Microsporidiosis is an opportunistic infection that produces chronic diarrhoea and cholangiopathy in patients with AIDS, mainly caused by two species of microsporidia, Enterocytozoon bieneusi and Encephalitozon intestinalis. The aim of this work was to develop an integral system for the diagnosis of microsporidiosis of the intestine and biliary tract in HIVinfected patients, comprising microscopic and molecular techniques. Methods The study population comprised 143 adult patients of both sexes with diagnosis of HIV infection, with chronic diarrhoea, and with or without HIV-associated cholangiopathy. Stool studies for microsporidia identifcation of spores were performed on each patient. A video esofagogastroduodenoscopy with biopsy collection was also carried out for routine histology and semi-thin sections stained with Azure II. Species identifcation was carried out by transmission electron microscopy and/or polymerase chain reaction for the species E. bieneusi and E. intestinalis. Results Out of the 143 patients a total of 12.6% (n=18) were infected with microsporidia. Microsporidia species identifed in most cases was E. bieneusi (16/18 cases), followed by E. intestinalis (4/18), all of these last ones in coinfection with E. bieneusi. Conclusions Clinical, imaging, microscopic and molecular analyses, when applied in a systematic and integrated approach, allow diagnosis and identifcation of microsporidia at species level in AIDS patients with chronic diarrhoea, and with or without HIV-associated cholangiopathy.Fil: Velásquez, Jorge Néstor. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; ArgentinaFil: Di Risio, Cecilia Diana. Hospital Municipal General de Agudos Doctor José Penna; ArgentinaFil: Etchart, Cristina. Hospital Municipal General de Agudos Doctor José Penna; ArgentinaFil: Chertcoff, Agustín Víctor. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Astudillo, Osvaldo Germán. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Carnevale, Silvana. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaVersita2019-07-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/122862Velásquez, Jorge Néstor; Di Risio, Cecilia Diana; Etchart, Cristina; Chertcoff, Agustín Víctor; Astudillo, Osvaldo Germán; et al.; Multimethodological approach to gastrointestinal microsporidiosis in HIV-infected patients; Versita; Acta Parasitologica; 64; 3; 8-7-2019; 658-6691230-28211896-1851CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.2478/s11686-019-00095-zinfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.2478%2Fs11686-019-00095-zinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:16Zoai:ri.conicet.gov.ar:11336/122862instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:16.206CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Multimethodological approach to gastrointestinal microsporidiosis in HIV-infected patients
title Multimethodological approach to gastrointestinal microsporidiosis in HIV-infected patients
spellingShingle Multimethodological approach to gastrointestinal microsporidiosis in HIV-infected patients
Velásquez, Jorge Néstor
ENCEPHALITOZOON INTESTINALIS
ENTEROCYTOZOON BIENEUSI
HIV
MICROSPORIDIOSIS
title_short Multimethodological approach to gastrointestinal microsporidiosis in HIV-infected patients
title_full Multimethodological approach to gastrointestinal microsporidiosis in HIV-infected patients
title_fullStr Multimethodological approach to gastrointestinal microsporidiosis in HIV-infected patients
title_full_unstemmed Multimethodological approach to gastrointestinal microsporidiosis in HIV-infected patients
title_sort Multimethodological approach to gastrointestinal microsporidiosis in HIV-infected patients
dc.creator.none.fl_str_mv Velásquez, Jorge Néstor
Di Risio, Cecilia Diana
Etchart, Cristina
Chertcoff, Agustín Víctor
Astudillo, Osvaldo Germán
Carnevale, Silvana
author Velásquez, Jorge Néstor
author_facet Velásquez, Jorge Néstor
Di Risio, Cecilia Diana
Etchart, Cristina
Chertcoff, Agustín Víctor
Astudillo, Osvaldo Germán
Carnevale, Silvana
author_role author
author2 Di Risio, Cecilia Diana
Etchart, Cristina
Chertcoff, Agustín Víctor
Astudillo, Osvaldo Germán
Carnevale, Silvana
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ENCEPHALITOZOON INTESTINALIS
ENTEROCYTOZOON BIENEUSI
HIV
MICROSPORIDIOSIS
topic ENCEPHALITOZOON INTESTINALIS
ENTEROCYTOZOON BIENEUSI
HIV
MICROSPORIDIOSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Purpose Microsporidiosis is an opportunistic infection that produces chronic diarrhoea and cholangiopathy in patients with AIDS, mainly caused by two species of microsporidia, Enterocytozoon bieneusi and Encephalitozon intestinalis. The aim of this work was to develop an integral system for the diagnosis of microsporidiosis of the intestine and biliary tract in HIVinfected patients, comprising microscopic and molecular techniques. Methods The study population comprised 143 adult patients of both sexes with diagnosis of HIV infection, with chronic diarrhoea, and with or without HIV-associated cholangiopathy. Stool studies for microsporidia identifcation of spores were performed on each patient. A video esofagogastroduodenoscopy with biopsy collection was also carried out for routine histology and semi-thin sections stained with Azure II. Species identifcation was carried out by transmission electron microscopy and/or polymerase chain reaction for the species E. bieneusi and E. intestinalis. Results Out of the 143 patients a total of 12.6% (n=18) were infected with microsporidia. Microsporidia species identifed in most cases was E. bieneusi (16/18 cases), followed by E. intestinalis (4/18), all of these last ones in coinfection with E. bieneusi. Conclusions Clinical, imaging, microscopic and molecular analyses, when applied in a systematic and integrated approach, allow diagnosis and identifcation of microsporidia at species level in AIDS patients with chronic diarrhoea, and with or without HIV-associated cholangiopathy.
Fil: Velásquez, Jorge Néstor. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina
Fil: Di Risio, Cecilia Diana. Hospital Municipal General de Agudos Doctor José Penna; Argentina
Fil: Etchart, Cristina. Hospital Municipal General de Agudos Doctor José Penna; Argentina
Fil: Chertcoff, Agustín Víctor. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Astudillo, Osvaldo Germán. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Carnevale, Silvana. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Purpose Microsporidiosis is an opportunistic infection that produces chronic diarrhoea and cholangiopathy in patients with AIDS, mainly caused by two species of microsporidia, Enterocytozoon bieneusi and Encephalitozon intestinalis. The aim of this work was to develop an integral system for the diagnosis of microsporidiosis of the intestine and biliary tract in HIVinfected patients, comprising microscopic and molecular techniques. Methods The study population comprised 143 adult patients of both sexes with diagnosis of HIV infection, with chronic diarrhoea, and with or without HIV-associated cholangiopathy. Stool studies for microsporidia identifcation of spores were performed on each patient. A video esofagogastroduodenoscopy with biopsy collection was also carried out for routine histology and semi-thin sections stained with Azure II. Species identifcation was carried out by transmission electron microscopy and/or polymerase chain reaction for the species E. bieneusi and E. intestinalis. Results Out of the 143 patients a total of 12.6% (n=18) were infected with microsporidia. Microsporidia species identifed in most cases was E. bieneusi (16/18 cases), followed by E. intestinalis (4/18), all of these last ones in coinfection with E. bieneusi. Conclusions Clinical, imaging, microscopic and molecular analyses, when applied in a systematic and integrated approach, allow diagnosis and identifcation of microsporidia at species level in AIDS patients with chronic diarrhoea, and with or without HIV-associated cholangiopathy.
publishDate 2019
dc.date.none.fl_str_mv 2019-07-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/122862
Velásquez, Jorge Néstor; Di Risio, Cecilia Diana; Etchart, Cristina; Chertcoff, Agustín Víctor; Astudillo, Osvaldo Germán; et al.; Multimethodological approach to gastrointestinal microsporidiosis in HIV-infected patients; Versita; Acta Parasitologica; 64; 3; 8-7-2019; 658-669
1230-2821
1896-1851
CONICET Digital
CONICET
url http://hdl.handle.net/11336/122862
identifier_str_mv Velásquez, Jorge Néstor; Di Risio, Cecilia Diana; Etchart, Cristina; Chertcoff, Agustín Víctor; Astudillo, Osvaldo Germán; et al.; Multimethodological approach to gastrointestinal microsporidiosis in HIV-infected patients; Versita; Acta Parasitologica; 64; 3; 8-7-2019; 658-669
1230-2821
1896-1851
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.2478/s11686-019-00095-z
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.2478%2Fs11686-019-00095-z
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Versita
publisher.none.fl_str_mv Versita
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269902299201536
score 13.13397

Publicaciones similares