Effect of serum lipoproteins and cholesterol on an exogenous pulmonary surfactant: ESR analysis of structural changes and their relation with surfactant activity

Autores
Martínez Sarrasague, María Margarita; Cimato, Alejandra Noemí; Piehl, Lidia Leonor; Brites, Fernando Daniel; Facorro, Graciela
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The study of structural changes in the surfactant may help to understand the mechanisms by which the surfactant is inactivated by serum. Here, we compared the in vitro effects of serum, albumin, lipoproteins (VLDL, LDL, HDL) and cholesterol on the dynamic and structural properties of surfactant suspensions by electronic spin resonance and surface tension measurements. Our results showed that albumin seems to be responsible for macrostructure disaggregation and increased rigidity in the hydrophobic region, but it did not affect surfactant activity. Fluidity in the polar area seems to be critical for proper physiological activity, and the changes induced by serum observed in this area would be generated by HDL or cholesterol, but the amount of cholesterol transferred by serum is not significant. Statistical analysis showed that surfactant activity correlated with the fluidity in the polar area but not with that in the hydrophobic region. We obtained strong evidence that among all the serum components tested, HDL is the one that causes the structural changes that compromise surfactant performance.
Fil: Martínez Sarrasague, María Margarita. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Fisicomatemática. Cátedra de Física; Argentina
Fil: Cimato, Alejandra Noemí. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Fisicomatemática. Cátedra de Física; Argentina
Fil: Piehl, Lidia Leonor. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Fisicomatemática. Cátedra de Física; Argentina
Fil: Brites, Fernando Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Facorro, Graciela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Fisicomatemática. Cátedra de Física; Argentina
Materia
Exogenous Pulmonary Surfactant
Serum Lipoproteins
Structural Changes
Cholesterol
Esr
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14169
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/14169
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of serum lipoproteins and cholesterol on an exogenous pulmonary surfactant: ESR analysis of structural changes and their relation with surfactant activityMartínez Sarrasague, María MargaritaCimato, Alejandra NoemíPiehl, Lidia LeonorBrites, Fernando DanielFacorro, GracielaExogenous Pulmonary SurfactantSerum LipoproteinsStructural ChangesCholesterolEsrhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The study of structural changes in the surfactant may help to understand the mechanisms by which the surfactant is inactivated by serum. Here, we compared the in vitro effects of serum, albumin, lipoproteins (VLDL, LDL, HDL) and cholesterol on the dynamic and structural properties of surfactant suspensions by electronic spin resonance and surface tension measurements. Our results showed that albumin seems to be responsible for macrostructure disaggregation and increased rigidity in the hydrophobic region, but it did not affect surfactant activity. Fluidity in the polar area seems to be critical for proper physiological activity, and the changes induced by serum observed in this area would be generated by HDL or cholesterol, but the amount of cholesterol transferred by serum is not significant. Statistical analysis showed that surfactant activity correlated with the fluidity in the polar area but not with that in the hydrophobic region. We obtained strong evidence that among all the serum components tested, HDL is the one that causes the structural changes that compromise surfactant performance.Fil: Martínez Sarrasague, María Margarita. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Fisicomatemática. Cátedra de Física; ArgentinaFil: Cimato, Alejandra Noemí. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Fisicomatemática. Cátedra de Física; ArgentinaFil: Piehl, Lidia Leonor. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Fisicomatemática. Cátedra de Física; ArgentinaFil: Brites, Fernando Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Facorro, Graciela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Fisicomatemática. Cátedra de Física; ArgentinaElsevier Science2013-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14169Martínez Sarrasague, María Margarita; Cimato, Alejandra Noemí; Piehl, Lidia Leonor; Brites, Fernando Daniel; Facorro, Graciela; Effect of serum lipoproteins and cholesterol on an exogenous pulmonary surfactant: ESR analysis of structural changes and their relation with surfactant activity; Elsevier Science; Respiratory Physiology & Neurobiology; 189; 3; 12-2013; 581-5871569-9048enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1569904813002759info:eu-repo/semantics/altIdentifier/doi/10.1016/j.resp.2013.08.004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:02:00Zoai:ri.conicet.gov.ar:11336/14169instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:02:01.188CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of serum lipoproteins and cholesterol on an exogenous pulmonary surfactant: ESR analysis of structural changes and their relation with surfactant activity
title Effect of serum lipoproteins and cholesterol on an exogenous pulmonary surfactant: ESR analysis of structural changes and their relation with surfactant activity
spellingShingle Effect of serum lipoproteins and cholesterol on an exogenous pulmonary surfactant: ESR analysis of structural changes and their relation with surfactant activity
Martínez Sarrasague, María Margarita
Exogenous Pulmonary Surfactant
Serum Lipoproteins
Structural Changes
Cholesterol
Esr
title_short Effect of serum lipoproteins and cholesterol on an exogenous pulmonary surfactant: ESR analysis of structural changes and their relation with surfactant activity
title_full Effect of serum lipoproteins and cholesterol on an exogenous pulmonary surfactant: ESR analysis of structural changes and their relation with surfactant activity
title_fullStr Effect of serum lipoproteins and cholesterol on an exogenous pulmonary surfactant: ESR analysis of structural changes and their relation with surfactant activity
title_full_unstemmed Effect of serum lipoproteins and cholesterol on an exogenous pulmonary surfactant: ESR analysis of structural changes and their relation with surfactant activity
title_sort Effect of serum lipoproteins and cholesterol on an exogenous pulmonary surfactant: ESR analysis of structural changes and their relation with surfactant activity
dc.creator.none.fl_str_mv Martínez Sarrasague, María Margarita
Cimato, Alejandra Noemí
Piehl, Lidia Leonor
Brites, Fernando Daniel
Facorro, Graciela
author Martínez Sarrasague, María Margarita
author_facet Martínez Sarrasague, María Margarita
Cimato, Alejandra Noemí
Piehl, Lidia Leonor
Brites, Fernando Daniel
Facorro, Graciela
author_role author
author2 Cimato, Alejandra Noemí
Piehl, Lidia Leonor
Brites, Fernando Daniel
Facorro, Graciela
author2_role author
author
author
author
dc.subject.none.fl_str_mv Exogenous Pulmonary Surfactant
Serum Lipoproteins
Structural Changes
Cholesterol
Esr
topic Exogenous Pulmonary Surfactant
Serum Lipoproteins
Structural Changes
Cholesterol
Esr
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The study of structural changes in the surfactant may help to understand the mechanisms by which the surfactant is inactivated by serum. Here, we compared the in vitro effects of serum, albumin, lipoproteins (VLDL, LDL, HDL) and cholesterol on the dynamic and structural properties of surfactant suspensions by electronic spin resonance and surface tension measurements. Our results showed that albumin seems to be responsible for macrostructure disaggregation and increased rigidity in the hydrophobic region, but it did not affect surfactant activity. Fluidity in the polar area seems to be critical for proper physiological activity, and the changes induced by serum observed in this area would be generated by HDL or cholesterol, but the amount of cholesterol transferred by serum is not significant. Statistical analysis showed that surfactant activity correlated with the fluidity in the polar area but not with that in the hydrophobic region. We obtained strong evidence that among all the serum components tested, HDL is the one that causes the structural changes that compromise surfactant performance.
Fil: Martínez Sarrasague, María Margarita. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Fisicomatemática. Cátedra de Física; Argentina
Fil: Cimato, Alejandra Noemí. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Fisicomatemática. Cátedra de Física; Argentina
Fil: Piehl, Lidia Leonor. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Fisicomatemática. Cátedra de Física; Argentina
Fil: Brites, Fernando Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Facorro, Graciela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Fisicomatemática. Cátedra de Física; Argentina
description The study of structural changes in the surfactant may help to understand the mechanisms by which the surfactant is inactivated by serum. Here, we compared the in vitro effects of serum, albumin, lipoproteins (VLDL, LDL, HDL) and cholesterol on the dynamic and structural properties of surfactant suspensions by electronic spin resonance and surface tension measurements. Our results showed that albumin seems to be responsible for macrostructure disaggregation and increased rigidity in the hydrophobic region, but it did not affect surfactant activity. Fluidity in the polar area seems to be critical for proper physiological activity, and the changes induced by serum observed in this area would be generated by HDL or cholesterol, but the amount of cholesterol transferred by serum is not significant. Statistical analysis showed that surfactant activity correlated with the fluidity in the polar area but not with that in the hydrophobic region. We obtained strong evidence that among all the serum components tested, HDL is the one that causes the structural changes that compromise surfactant performance.
publishDate 2013
dc.date.none.fl_str_mv 2013-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14169
Martínez Sarrasague, María Margarita; Cimato, Alejandra Noemí; Piehl, Lidia Leonor; Brites, Fernando Daniel; Facorro, Graciela; Effect of serum lipoproteins and cholesterol on an exogenous pulmonary surfactant: ESR analysis of structural changes and their relation with surfactant activity; Elsevier Science; Respiratory Physiology & Neurobiology; 189; 3; 12-2013; 581-587
1569-9048
url http://hdl.handle.net/11336/14169
identifier_str_mv Martínez Sarrasague, María Margarita; Cimato, Alejandra Noemí; Piehl, Lidia Leonor; Brites, Fernando Daniel; Facorro, Graciela; Effect of serum lipoproteins and cholesterol on an exogenous pulmonary surfactant: ESR analysis of structural changes and their relation with surfactant activity; Elsevier Science; Respiratory Physiology & Neurobiology; 189; 3; 12-2013; 581-587
1569-9048
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1569904813002759
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.resp.2013.08.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782354765381632
score 12.982451

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