Antimicrobial nanolayers of thymol and carvacrol on titanium surfaces: The crucial role of interfacial properties in thymol’s superior osteogenic response
- Autores
- Gonzalez, Ariel; Miñan, Alejandro Guillermo; Prieto, Eduardo Daniel; Schilardi, Patricia Laura; Fagali, Natalia Soledad; Fernandez Lorenzo, Monica Alicia
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- “Green’’ nanotechnologies have emerged as environmentally friendly alternatives against microbial multidrug-resistant biofilms. In this study, bactericidal “green” nanolayers (NL) were developed on Ti surfaces using two isomeric phytocompounds, carvacrol (Carv-Ti-NL) and thymol (TOH-Ti-NL). These NLs were fabricated using a one-step immersion treatment method based on a simple and spontaneous self-assembly process. Both NLs revealed strong antimicrobial activity, displaying anti-biofilm and biocidal effects. Notably, TOH-Ti-NL exhibited superior osteogenic performance compared to Carv-Ti-NL, as evidenced by enhanced pre-osteoblast cell attachment and growth, and the production of ALP, collagen type I and Ca2+ deposition. In contrast, fibroblastic cells exhibited reduced attachment on TOH-Ti-NL and enhanced proliferation on Carv-Ti-NL. Considering the biological differential effects, the physicochemical properties of these conformational isomers’ NLs were studied to elucidate potential differences that could impact on cell response. Although the ATR-FTIR spectra of the NLs were similar and indicated the spontaneous oxidation of Carv and TOH leading to ketonic structures, distinct contributions were observed after the electrooxidation of each NL. Slight differences in hydrophilicity were found for both nanostructures, but higher roughness was found for TOH-Ti-NL. Furthermore, the release curves of Carv and TOH from the NLs revealed distinct profiles over time. Overall, Carv and TOH formed self-assembled layers on Ti able to eradicate Staphylococcus aureus biofilms. Their different physical and chemical characteristics induced distinct responses from eukaryotic cells attached to the NLs. Given these characteristics one might envisage the use of either Carv-Ti-NL or TOH-Ti-NL in order to fine-tune specific chemical physical properties of Ti-based implants.
Fil: Gonzalez, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Miñan, Alejandro Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Prieto, Eduardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Schilardi, Patricia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Fagali, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Fernandez Lorenzo, Monica Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina - Materia
-
THYMOL
CARVACROL
NANOFILMS
GREEN TECHNOLOGY
ANTIMICROBIAL COATINGS
OSTEOGENIC ACTIVITY - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/264110
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spelling |
Antimicrobial nanolayers of thymol and carvacrol on titanium surfaces: The crucial role of interfacial properties in thymol’s superior osteogenic responseGonzalez, ArielMiñan, Alejandro GuillermoPrieto, Eduardo DanielSchilardi, Patricia LauraFagali, Natalia SoledadFernandez Lorenzo, Monica AliciaTHYMOLCARVACROLNANOFILMSGREEN TECHNOLOGYANTIMICROBIAL COATINGSOSTEOGENIC ACTIVITYhttps://purl.org/becyt/ford/2.5https://purl.org/becyt/ford/2https://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1“Green’’ nanotechnologies have emerged as environmentally friendly alternatives against microbial multidrug-resistant biofilms. In this study, bactericidal “green” nanolayers (NL) were developed on Ti surfaces using two isomeric phytocompounds, carvacrol (Carv-Ti-NL) and thymol (TOH-Ti-NL). These NLs were fabricated using a one-step immersion treatment method based on a simple and spontaneous self-assembly process. Both NLs revealed strong antimicrobial activity, displaying anti-biofilm and biocidal effects. Notably, TOH-Ti-NL exhibited superior osteogenic performance compared to Carv-Ti-NL, as evidenced by enhanced pre-osteoblast cell attachment and growth, and the production of ALP, collagen type I and Ca2+ deposition. In contrast, fibroblastic cells exhibited reduced attachment on TOH-Ti-NL and enhanced proliferation on Carv-Ti-NL. Considering the biological differential effects, the physicochemical properties of these conformational isomers’ NLs were studied to elucidate potential differences that could impact on cell response. Although the ATR-FTIR spectra of the NLs were similar and indicated the spontaneous oxidation of Carv and TOH leading to ketonic structures, distinct contributions were observed after the electrooxidation of each NL. Slight differences in hydrophilicity were found for both nanostructures, but higher roughness was found for TOH-Ti-NL. Furthermore, the release curves of Carv and TOH from the NLs revealed distinct profiles over time. Overall, Carv and TOH formed self-assembled layers on Ti able to eradicate Staphylococcus aureus biofilms. Their different physical and chemical characteristics induced distinct responses from eukaryotic cells attached to the NLs. Given these characteristics one might envisage the use of either Carv-Ti-NL or TOH-Ti-NL in order to fine-tune specific chemical physical properties of Ti-based implants.Fil: Gonzalez, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Miñan, Alejandro Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Prieto, Eduardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Schilardi, Patricia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Fagali, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Fernandez Lorenzo, Monica Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaCold Spring Harbor Laboratory Press2024-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/264110Gonzalez, Ariel; Miñan, Alejandro Guillermo; Prieto, Eduardo Daniel; Schilardi, Patricia Laura; Fagali, Natalia Soledad; et al.; Antimicrobial nanolayers of thymol and carvacrol on titanium surfaces: The crucial role of interfacial properties in thymol’s superior osteogenic response; Cold Spring Harbor Laboratory Press; bioRxiv; 8-2024; 1-442692-8205CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.biorxiv.org/content/10.1101/2024.08.30.610464v1info:eu-repo/semantics/altIdentifier/doi/10.1101/2024.08.30.610464info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:34:18Zoai:ri.conicet.gov.ar:11336/264110instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:34:19.007CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Antimicrobial nanolayers of thymol and carvacrol on titanium surfaces: The crucial role of interfacial properties in thymol’s superior osteogenic response |
title |
Antimicrobial nanolayers of thymol and carvacrol on titanium surfaces: The crucial role of interfacial properties in thymol’s superior osteogenic response |
spellingShingle |
Antimicrobial nanolayers of thymol and carvacrol on titanium surfaces: The crucial role of interfacial properties in thymol’s superior osteogenic response Gonzalez, Ariel THYMOL CARVACROL NANOFILMS GREEN TECHNOLOGY ANTIMICROBIAL COATINGS OSTEOGENIC ACTIVITY |
title_short |
Antimicrobial nanolayers of thymol and carvacrol on titanium surfaces: The crucial role of interfacial properties in thymol’s superior osteogenic response |
title_full |
Antimicrobial nanolayers of thymol and carvacrol on titanium surfaces: The crucial role of interfacial properties in thymol’s superior osteogenic response |
title_fullStr |
Antimicrobial nanolayers of thymol and carvacrol on titanium surfaces: The crucial role of interfacial properties in thymol’s superior osteogenic response |
title_full_unstemmed |
Antimicrobial nanolayers of thymol and carvacrol on titanium surfaces: The crucial role of interfacial properties in thymol’s superior osteogenic response |
title_sort |
Antimicrobial nanolayers of thymol and carvacrol on titanium surfaces: The crucial role of interfacial properties in thymol’s superior osteogenic response |
dc.creator.none.fl_str_mv |
Gonzalez, Ariel Miñan, Alejandro Guillermo Prieto, Eduardo Daniel Schilardi, Patricia Laura Fagali, Natalia Soledad Fernandez Lorenzo, Monica Alicia |
author |
Gonzalez, Ariel |
author_facet |
Gonzalez, Ariel Miñan, Alejandro Guillermo Prieto, Eduardo Daniel Schilardi, Patricia Laura Fagali, Natalia Soledad Fernandez Lorenzo, Monica Alicia |
author_role |
author |
author2 |
Miñan, Alejandro Guillermo Prieto, Eduardo Daniel Schilardi, Patricia Laura Fagali, Natalia Soledad Fernandez Lorenzo, Monica Alicia |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
THYMOL CARVACROL NANOFILMS GREEN TECHNOLOGY ANTIMICROBIAL COATINGS OSTEOGENIC ACTIVITY |
topic |
THYMOL CARVACROL NANOFILMS GREEN TECHNOLOGY ANTIMICROBIAL COATINGS OSTEOGENIC ACTIVITY |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.5 https://purl.org/becyt/ford/2 https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
“Green’’ nanotechnologies have emerged as environmentally friendly alternatives against microbial multidrug-resistant biofilms. In this study, bactericidal “green” nanolayers (NL) were developed on Ti surfaces using two isomeric phytocompounds, carvacrol (Carv-Ti-NL) and thymol (TOH-Ti-NL). These NLs were fabricated using a one-step immersion treatment method based on a simple and spontaneous self-assembly process. Both NLs revealed strong antimicrobial activity, displaying anti-biofilm and biocidal effects. Notably, TOH-Ti-NL exhibited superior osteogenic performance compared to Carv-Ti-NL, as evidenced by enhanced pre-osteoblast cell attachment and growth, and the production of ALP, collagen type I and Ca2+ deposition. In contrast, fibroblastic cells exhibited reduced attachment on TOH-Ti-NL and enhanced proliferation on Carv-Ti-NL. Considering the biological differential effects, the physicochemical properties of these conformational isomers’ NLs were studied to elucidate potential differences that could impact on cell response. Although the ATR-FTIR spectra of the NLs were similar and indicated the spontaneous oxidation of Carv and TOH leading to ketonic structures, distinct contributions were observed after the electrooxidation of each NL. Slight differences in hydrophilicity were found for both nanostructures, but higher roughness was found for TOH-Ti-NL. Furthermore, the release curves of Carv and TOH from the NLs revealed distinct profiles over time. Overall, Carv and TOH formed self-assembled layers on Ti able to eradicate Staphylococcus aureus biofilms. Their different physical and chemical characteristics induced distinct responses from eukaryotic cells attached to the NLs. Given these characteristics one might envisage the use of either Carv-Ti-NL or TOH-Ti-NL in order to fine-tune specific chemical physical properties of Ti-based implants. Fil: Gonzalez, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina Fil: Miñan, Alejandro Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina Fil: Prieto, Eduardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina Fil: Schilardi, Patricia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina Fil: Fagali, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina Fil: Fernandez Lorenzo, Monica Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina |
description |
“Green’’ nanotechnologies have emerged as environmentally friendly alternatives against microbial multidrug-resistant biofilms. In this study, bactericidal “green” nanolayers (NL) were developed on Ti surfaces using two isomeric phytocompounds, carvacrol (Carv-Ti-NL) and thymol (TOH-Ti-NL). These NLs were fabricated using a one-step immersion treatment method based on a simple and spontaneous self-assembly process. Both NLs revealed strong antimicrobial activity, displaying anti-biofilm and biocidal effects. Notably, TOH-Ti-NL exhibited superior osteogenic performance compared to Carv-Ti-NL, as evidenced by enhanced pre-osteoblast cell attachment and growth, and the production of ALP, collagen type I and Ca2+ deposition. In contrast, fibroblastic cells exhibited reduced attachment on TOH-Ti-NL and enhanced proliferation on Carv-Ti-NL. Considering the biological differential effects, the physicochemical properties of these conformational isomers’ NLs were studied to elucidate potential differences that could impact on cell response. Although the ATR-FTIR spectra of the NLs were similar and indicated the spontaneous oxidation of Carv and TOH leading to ketonic structures, distinct contributions were observed after the electrooxidation of each NL. Slight differences in hydrophilicity were found for both nanostructures, but higher roughness was found for TOH-Ti-NL. Furthermore, the release curves of Carv and TOH from the NLs revealed distinct profiles over time. Overall, Carv and TOH formed self-assembled layers on Ti able to eradicate Staphylococcus aureus biofilms. Their different physical and chemical characteristics induced distinct responses from eukaryotic cells attached to the NLs. Given these characteristics one might envisage the use of either Carv-Ti-NL or TOH-Ti-NL in order to fine-tune specific chemical physical properties of Ti-based implants. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/264110 Gonzalez, Ariel; Miñan, Alejandro Guillermo; Prieto, Eduardo Daniel; Schilardi, Patricia Laura; Fagali, Natalia Soledad; et al.; Antimicrobial nanolayers of thymol and carvacrol on titanium surfaces: The crucial role of interfacial properties in thymol’s superior osteogenic response; Cold Spring Harbor Laboratory Press; bioRxiv; 8-2024; 1-44 2692-8205 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/264110 |
identifier_str_mv |
Gonzalez, Ariel; Miñan, Alejandro Guillermo; Prieto, Eduardo Daniel; Schilardi, Patricia Laura; Fagali, Natalia Soledad; et al.; Antimicrobial nanolayers of thymol and carvacrol on titanium surfaces: The crucial role of interfacial properties in thymol’s superior osteogenic response; Cold Spring Harbor Laboratory Press; bioRxiv; 8-2024; 1-44 2692-8205 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.biorxiv.org/content/10.1101/2024.08.30.610464v1 info:eu-repo/semantics/altIdentifier/doi/10.1101/2024.08.30.610464 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Cold Spring Harbor Laboratory Press |
publisher.none.fl_str_mv |
Cold Spring Harbor Laboratory Press |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614359743987712 |
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13.070432 |