Morphological and Functional Alterations in Zebrafish (Danio rerio) Liver after Exposure to Two Ecologically Relevant Concentrations of Lead

Autores
Macirella, Rachele; Curcio, Vittoria; Ahmed, Abdalmoiz I. M.; Talarico, Federica; Sesti, Settimio; Paravani, Enrique Valentin; Odetti, Lucia Magdalena; Mezzasalma, Marcello; Brunelli, Elvira
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lead (Pb) is a non-essential, highly toxic, and persistent element widely recognized as one of the most concerning pollutants. It is listed on the Priority List of Hazardous Substances. Widespread environmental contamination from Pb is a serious issue for human health and wildlife. In fish, Pb mainly accumulates in the liver, which is a key component for metal detoxification and excretion processes. In this study, we investigated, for the first time, the morphological and functional injuries induced in zebrafish (Danio rerio) liver by two very low and environmentally relevant concentrations of Pb (2.5 and 5 μg/L) after 48, 96, and 192 h of exposure. We observed significant histological alterations in all the exposed samples, and it was demonstrated that the extent of injuries increased with dose and exposure time. The most common modifications observed were congestion of blood vessels and sinusoids, cytoplasmic vacuolizations, parenchyma dyschromia, and macrophage proliferation. Pb administration also resulted in a significant increase in lipid content and the upregulation of key genes that are involved in metal detoxification (mtf1) and the defensive response against oxidative stress (sod1 and cat). We show that even very low doses of Pb can disrupt liver morphology and function.
Fil: Macirella, Rachele. Università della Calabria; Italia
Fil: Curcio, Vittoria. Università della Calabria; Italia
Fil: Ahmed, Abdalmoiz I. M.. Università della Calabria; Italia
Fil: Talarico, Federica. Università della Calabria; Italia
Fil: Sesti, Settimio. Università della Calabria; Italia
Fil: Paravani, Enrique Valentin. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina
Fil: Odetti, Lucia Magdalena. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Cátedra de Toxicología y Bioquímica Legal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Mezzasalma, Marcello. Università della Calabria; Italia
Fil: Brunelli, Elvira. Università della Calabria; Italia
Materia
CAT
HISTOLOGICAL BIOMARKERS
LIVER
MOLECULAR BIOMARKERS
MTS
PB
SOD
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/225026
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/225026
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Morphological and Functional Alterations in Zebrafish (Danio rerio) Liver after Exposure to Two Ecologically Relevant Concentrations of LeadMacirella, RacheleCurcio, VittoriaAhmed, Abdalmoiz I. M.Talarico, FedericaSesti, SettimioParavani, Enrique ValentinOdetti, Lucia MagdalenaMezzasalma, MarcelloBrunelli, ElviraCATHISTOLOGICAL BIOMARKERSLIVERMOLECULAR BIOMARKERSMTSPBSODhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Lead (Pb) is a non-essential, highly toxic, and persistent element widely recognized as one of the most concerning pollutants. It is listed on the Priority List of Hazardous Substances. Widespread environmental contamination from Pb is a serious issue for human health and wildlife. In fish, Pb mainly accumulates in the liver, which is a key component for metal detoxification and excretion processes. In this study, we investigated, for the first time, the morphological and functional injuries induced in zebrafish (Danio rerio) liver by two very low and environmentally relevant concentrations of Pb (2.5 and 5 μg/L) after 48, 96, and 192 h of exposure. We observed significant histological alterations in all the exposed samples, and it was demonstrated that the extent of injuries increased with dose and exposure time. The most common modifications observed were congestion of blood vessels and sinusoids, cytoplasmic vacuolizations, parenchyma dyschromia, and macrophage proliferation. Pb administration also resulted in a significant increase in lipid content and the upregulation of key genes that are involved in metal detoxification (mtf1) and the defensive response against oxidative stress (sod1 and cat). We show that even very low doses of Pb can disrupt liver morphology and function.Fil: Macirella, Rachele. Università della Calabria; ItaliaFil: Curcio, Vittoria. Università della Calabria; ItaliaFil: Ahmed, Abdalmoiz I. M.. Università della Calabria; ItaliaFil: Talarico, Federica. Università della Calabria; ItaliaFil: Sesti, Settimio. Università della Calabria; ItaliaFil: Paravani, Enrique Valentin. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; ArgentinaFil: Odetti, Lucia Magdalena. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Cátedra de Toxicología y Bioquímica Legal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Mezzasalma, Marcello. Università della Calabria; ItaliaFil: Brunelli, Elvira. Università della Calabria; ItaliaMDPI2023-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/225026Macirella, Rachele; Curcio, Vittoria; Ahmed, Abdalmoiz I. M.; Talarico, Federica; Sesti, Settimio; et al.; Morphological and Functional Alterations in Zebrafish (Danio rerio) Liver after Exposure to Two Ecologically Relevant Concentrations of Lead; MDPI; Fishes; 8; 7; 6-2023; 1-172410-3888CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2410-3888/8/7/342info:eu-repo/semantics/altIdentifier/doi/10.3390/fishes8070342info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-04-08T11:30:56Zoai:ri.conicet.gov.ar:11336/225026instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-04-08 11:30:56.948CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Morphological and Functional Alterations in Zebrafish (Danio rerio) Liver after Exposure to Two Ecologically Relevant Concentrations of Lead
title Morphological and Functional Alterations in Zebrafish (Danio rerio) Liver after Exposure to Two Ecologically Relevant Concentrations of Lead
spellingShingle Morphological and Functional Alterations in Zebrafish (Danio rerio) Liver after Exposure to Two Ecologically Relevant Concentrations of Lead
Macirella, Rachele
CAT
HISTOLOGICAL BIOMARKERS
LIVER
MOLECULAR BIOMARKERS
MTS
PB
SOD
title_short Morphological and Functional Alterations in Zebrafish (Danio rerio) Liver after Exposure to Two Ecologically Relevant Concentrations of Lead
title_full Morphological and Functional Alterations in Zebrafish (Danio rerio) Liver after Exposure to Two Ecologically Relevant Concentrations of Lead
title_fullStr Morphological and Functional Alterations in Zebrafish (Danio rerio) Liver after Exposure to Two Ecologically Relevant Concentrations of Lead
title_full_unstemmed Morphological and Functional Alterations in Zebrafish (Danio rerio) Liver after Exposure to Two Ecologically Relevant Concentrations of Lead
title_sort Morphological and Functional Alterations in Zebrafish (Danio rerio) Liver after Exposure to Two Ecologically Relevant Concentrations of Lead
dc.creator.none.fl_str_mv Macirella, Rachele
Curcio, Vittoria
Ahmed, Abdalmoiz I. M.
Talarico, Federica
Sesti, Settimio
Paravani, Enrique Valentin
Odetti, Lucia Magdalena
Mezzasalma, Marcello
Brunelli, Elvira
author Macirella, Rachele
author_facet Macirella, Rachele
Curcio, Vittoria
Ahmed, Abdalmoiz I. M.
Talarico, Federica
Sesti, Settimio
Paravani, Enrique Valentin
Odetti, Lucia Magdalena
Mezzasalma, Marcello
Brunelli, Elvira
author_role author
author2 Curcio, Vittoria
Ahmed, Abdalmoiz I. M.
Talarico, Federica
Sesti, Settimio
Paravani, Enrique Valentin
Odetti, Lucia Magdalena
Mezzasalma, Marcello
Brunelli, Elvira
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CAT
HISTOLOGICAL BIOMARKERS
LIVER
MOLECULAR BIOMARKERS
MTS
PB
SOD
topic CAT
HISTOLOGICAL BIOMARKERS
LIVER
MOLECULAR BIOMARKERS
MTS
PB
SOD
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Lead (Pb) is a non-essential, highly toxic, and persistent element widely recognized as one of the most concerning pollutants. It is listed on the Priority List of Hazardous Substances. Widespread environmental contamination from Pb is a serious issue for human health and wildlife. In fish, Pb mainly accumulates in the liver, which is a key component for metal detoxification and excretion processes. In this study, we investigated, for the first time, the morphological and functional injuries induced in zebrafish (Danio rerio) liver by two very low and environmentally relevant concentrations of Pb (2.5 and 5 μg/L) after 48, 96, and 192 h of exposure. We observed significant histological alterations in all the exposed samples, and it was demonstrated that the extent of injuries increased with dose and exposure time. The most common modifications observed were congestion of blood vessels and sinusoids, cytoplasmic vacuolizations, parenchyma dyschromia, and macrophage proliferation. Pb administration also resulted in a significant increase in lipid content and the upregulation of key genes that are involved in metal detoxification (mtf1) and the defensive response against oxidative stress (sod1 and cat). We show that even very low doses of Pb can disrupt liver morphology and function.
Fil: Macirella, Rachele. Università della Calabria; Italia
Fil: Curcio, Vittoria. Università della Calabria; Italia
Fil: Ahmed, Abdalmoiz I. M.. Università della Calabria; Italia
Fil: Talarico, Federica. Università della Calabria; Italia
Fil: Sesti, Settimio. Università della Calabria; Italia
Fil: Paravani, Enrique Valentin. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina
Fil: Odetti, Lucia Magdalena. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Cátedra de Toxicología y Bioquímica Legal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Mezzasalma, Marcello. Università della Calabria; Italia
Fil: Brunelli, Elvira. Università della Calabria; Italia
description Lead (Pb) is a non-essential, highly toxic, and persistent element widely recognized as one of the most concerning pollutants. It is listed on the Priority List of Hazardous Substances. Widespread environmental contamination from Pb is a serious issue for human health and wildlife. In fish, Pb mainly accumulates in the liver, which is a key component for metal detoxification and excretion processes. In this study, we investigated, for the first time, the morphological and functional injuries induced in zebrafish (Danio rerio) liver by two very low and environmentally relevant concentrations of Pb (2.5 and 5 μg/L) after 48, 96, and 192 h of exposure. We observed significant histological alterations in all the exposed samples, and it was demonstrated that the extent of injuries increased with dose and exposure time. The most common modifications observed were congestion of blood vessels and sinusoids, cytoplasmic vacuolizations, parenchyma dyschromia, and macrophage proliferation. Pb administration also resulted in a significant increase in lipid content and the upregulation of key genes that are involved in metal detoxification (mtf1) and the defensive response against oxidative stress (sod1 and cat). We show that even very low doses of Pb can disrupt liver morphology and function.
publishDate 2023
dc.date.none.fl_str_mv 2023-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/225026
Macirella, Rachele; Curcio, Vittoria; Ahmed, Abdalmoiz I. M.; Talarico, Federica; Sesti, Settimio; et al.; Morphological and Functional Alterations in Zebrafish (Danio rerio) Liver after Exposure to Two Ecologically Relevant Concentrations of Lead; MDPI; Fishes; 8; 7; 6-2023; 1-17
2410-3888
CONICET Digital
CONICET
url http://hdl.handle.net/11336/225026
identifier_str_mv Macirella, Rachele; Curcio, Vittoria; Ahmed, Abdalmoiz I. M.; Talarico, Federica; Sesti, Settimio; et al.; Morphological and Functional Alterations in Zebrafish (Danio rerio) Liver after Exposure to Two Ecologically Relevant Concentrations of Lead; MDPI; Fishes; 8; 7; 6-2023; 1-17
2410-3888
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2410-3888/8/7/342
info:eu-repo/semantics/altIdentifier/doi/10.3390/fishes8070342
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.018236

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