Nuclear factor kappa B-dependent Zif268 expression in hippocampus is required for recognition memory in mice

Autores
Zalcman, Gisela Patricia; Federman, Maria Noel; de la Fuente, Verónica; Romano, Arturo Gabriel
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Long-term memory formation requires gene expression after acquisition of new information. The first step in the regulation of gene expression is the participation of transcription factors (TFs) such as nuclear factor kappa B (NF-kB), which are present before the neuronal activity induced by training. It was proposed that the activation of these types of TFs allows a second step in gene regulation by induction of immediate-early genes (IEGs) whose protein products are, in turn, TFs. Between these IEGs, zif268 has been found to play a critical role in long-term memory formation and reprocessing after retrieval. Here we found in mice hippocampus that, on one hand, NF-kB was activated 45. min after training in a novel object recognition (NOR) task and that inhibiting NF-kB immediately after training by intrahippocampal administration of NF-kB Decoy DNA impaired NOR memory consolidation. On the other hand, Zif268 protein expression was induced 45. min after NOR training and the administration of DNA antisense to its mRNA post-training impaired recognition memory. Finally, we found that the inhibition of NF-kB by NF-kB Decoy DNA reduced significantly the training-induced Zif268 increment, indicating that NF-kB is involved in the regulation of Zif268 expression. Thus, the present results support the involvement of NF-kB activity-dependent Zif268 expression in the hippocampus during recognition memory consolidation.
Fil: Zalcman, Gisela Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Federman, Maria Noel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: de la Fuente, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Romano, Arturo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Materia
Memory Consolidation
Nf-ΚB
Transcription Factors
Zif268
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/60287
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/60287
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Nuclear factor kappa B-dependent Zif268 expression in hippocampus is required for recognition memory in miceZalcman, Gisela PatriciaFederman, Maria Noelde la Fuente, VerónicaRomano, Arturo GabrielMemory ConsolidationNf-ΚBTranscription FactorsZif268https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Long-term memory formation requires gene expression after acquisition of new information. The first step in the regulation of gene expression is the participation of transcription factors (TFs) such as nuclear factor kappa B (NF-kB), which are present before the neuronal activity induced by training. It was proposed that the activation of these types of TFs allows a second step in gene regulation by induction of immediate-early genes (IEGs) whose protein products are, in turn, TFs. Between these IEGs, zif268 has been found to play a critical role in long-term memory formation and reprocessing after retrieval. Here we found in mice hippocampus that, on one hand, NF-kB was activated 45. min after training in a novel object recognition (NOR) task and that inhibiting NF-kB immediately after training by intrahippocampal administration of NF-kB Decoy DNA impaired NOR memory consolidation. On the other hand, Zif268 protein expression was induced 45. min after NOR training and the administration of DNA antisense to its mRNA post-training impaired recognition memory. Finally, we found that the inhibition of NF-kB by NF-kB Decoy DNA reduced significantly the training-induced Zif268 increment, indicating that NF-kB is involved in the regulation of Zif268 expression. Thus, the present results support the involvement of NF-kB activity-dependent Zif268 expression in the hippocampus during recognition memory consolidation.Fil: Zalcman, Gisela Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Federman, Maria Noel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: de la Fuente, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Romano, Arturo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaAcademic Press Inc Elsevier Science2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/60287Zalcman, Gisela Patricia; Federman, Maria Noel; de la Fuente, Verónica; Romano, Arturo Gabriel; Nuclear factor kappa B-dependent Zif268 expression in hippocampus is required for recognition memory in mice; Academic Press Inc Elsevier Science; Neurobiology of Learning and Memory; 119; 3-2015; 10-171074-7427CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.nlm.2014.12.013info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1074742715000027info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:47:43Zoai:ri.conicet.gov.ar:11336/60287instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:47:43.449CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nuclear factor kappa B-dependent Zif268 expression in hippocampus is required for recognition memory in mice
title Nuclear factor kappa B-dependent Zif268 expression in hippocampus is required for recognition memory in mice
spellingShingle Nuclear factor kappa B-dependent Zif268 expression in hippocampus is required for recognition memory in mice
Zalcman, Gisela Patricia
Memory Consolidation
Nf-ΚB
Transcription Factors
Zif268
title_short Nuclear factor kappa B-dependent Zif268 expression in hippocampus is required for recognition memory in mice
title_full Nuclear factor kappa B-dependent Zif268 expression in hippocampus is required for recognition memory in mice
title_fullStr Nuclear factor kappa B-dependent Zif268 expression in hippocampus is required for recognition memory in mice
title_full_unstemmed Nuclear factor kappa B-dependent Zif268 expression in hippocampus is required for recognition memory in mice
title_sort Nuclear factor kappa B-dependent Zif268 expression in hippocampus is required for recognition memory in mice
dc.creator.none.fl_str_mv Zalcman, Gisela Patricia
Federman, Maria Noel
de la Fuente, Verónica
Romano, Arturo Gabriel
author Zalcman, Gisela Patricia
author_facet Zalcman, Gisela Patricia
Federman, Maria Noel
de la Fuente, Verónica
Romano, Arturo Gabriel
author_role author
author2 Federman, Maria Noel
de la Fuente, Verónica
Romano, Arturo Gabriel
author2_role author
author
author
dc.subject.none.fl_str_mv Memory Consolidation
Nf-ΚB
Transcription Factors
Zif268
topic Memory Consolidation
Nf-ΚB
Transcription Factors
Zif268
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Long-term memory formation requires gene expression after acquisition of new information. The first step in the regulation of gene expression is the participation of transcription factors (TFs) such as nuclear factor kappa B (NF-kB), which are present before the neuronal activity induced by training. It was proposed that the activation of these types of TFs allows a second step in gene regulation by induction of immediate-early genes (IEGs) whose protein products are, in turn, TFs. Between these IEGs, zif268 has been found to play a critical role in long-term memory formation and reprocessing after retrieval. Here we found in mice hippocampus that, on one hand, NF-kB was activated 45. min after training in a novel object recognition (NOR) task and that inhibiting NF-kB immediately after training by intrahippocampal administration of NF-kB Decoy DNA impaired NOR memory consolidation. On the other hand, Zif268 protein expression was induced 45. min after NOR training and the administration of DNA antisense to its mRNA post-training impaired recognition memory. Finally, we found that the inhibition of NF-kB by NF-kB Decoy DNA reduced significantly the training-induced Zif268 increment, indicating that NF-kB is involved in the regulation of Zif268 expression. Thus, the present results support the involvement of NF-kB activity-dependent Zif268 expression in the hippocampus during recognition memory consolidation.
Fil: Zalcman, Gisela Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Federman, Maria Noel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: de la Fuente, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Romano, Arturo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
description Long-term memory formation requires gene expression after acquisition of new information. The first step in the regulation of gene expression is the participation of transcription factors (TFs) such as nuclear factor kappa B (NF-kB), which are present before the neuronal activity induced by training. It was proposed that the activation of these types of TFs allows a second step in gene regulation by induction of immediate-early genes (IEGs) whose protein products are, in turn, TFs. Between these IEGs, zif268 has been found to play a critical role in long-term memory formation and reprocessing after retrieval. Here we found in mice hippocampus that, on one hand, NF-kB was activated 45. min after training in a novel object recognition (NOR) task and that inhibiting NF-kB immediately after training by intrahippocampal administration of NF-kB Decoy DNA impaired NOR memory consolidation. On the other hand, Zif268 protein expression was induced 45. min after NOR training and the administration of DNA antisense to its mRNA post-training impaired recognition memory. Finally, we found that the inhibition of NF-kB by NF-kB Decoy DNA reduced significantly the training-induced Zif268 increment, indicating that NF-kB is involved in the regulation of Zif268 expression. Thus, the present results support the involvement of NF-kB activity-dependent Zif268 expression in the hippocampus during recognition memory consolidation.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/60287
Zalcman, Gisela Patricia; Federman, Maria Noel; de la Fuente, Verónica; Romano, Arturo Gabriel; Nuclear factor kappa B-dependent Zif268 expression in hippocampus is required for recognition memory in mice; Academic Press Inc Elsevier Science; Neurobiology of Learning and Memory; 119; 3-2015; 10-17
1074-7427
CONICET Digital
CONICET
url http://hdl.handle.net/11336/60287
identifier_str_mv Zalcman, Gisela Patricia; Federman, Maria Noel; de la Fuente, Verónica; Romano, Arturo Gabriel; Nuclear factor kappa B-dependent Zif268 expression in hippocampus is required for recognition memory in mice; Academic Press Inc Elsevier Science; Neurobiology of Learning and Memory; 119; 3-2015; 10-17
1074-7427
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.nlm.2014.12.013
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1074742715000027
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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