Melatonin normalizes clinical and biochemical parameters of mild inflammation in diet-induced metabolic syndrome in rats

Autores
Cano Barquilla, Pilar; Pagano, Eleonora Samanta; Jiménez Ortega, Vanesa; Fernández Mateos, Pilar; Esquifino, Ana I.; Cardinali, Daniel Pedro
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objective of this study was to evaluate the efficacy of melatonin to affect mild inflammation in the metabolic syndrome (MS) induced by a high-fat diet in rats. Adult Wistar male rats were divided into four groups (n = 16/group): (i) control diet (3% fat); (ii) high-fat (35%) diet; (iii) high-fat diet + melatonin; and (iv) melatonin. Rats had free access to high-fat or control chow and one of the following drinking solutions for 10 wk: (a) tap water; (b) 25 μg/mL of melatonin. Plasma interleukin (IL)-1β, IL-4, IL-6, IL-10, tumor necrosis factor (TNF)-α, interferon (IFN)-γ, and C-reactive protein (CRP) were measured at two time intervals, that is, the middle of daylight period and the middle of the scotophase. In addition, a number of somatic and metabolic components employed clinically to monitor the MS were measured. Melatonin decreased the augmented circulating levels of IL-1β, IL-6, TNF-α, IFN-γ, and CRP seen in obese rats and restored the depressed levels of IL-4 and IL-10. Rats fed with the high-fat diet showed significantly higher body weights and augmented systolic blood pressure from the third and fourth week onwards, respectively, melatonin effectively preventing these changes. In high-fat-fed rats, circulating low-density lipoprotein-cholesterol, total cholesterol, and triglyceride concentration augmented significantly, melatonin being effective to counteract these changes. Melatonin-treated rats showed a decreased insulin resistance, the highest values of plasma high-density lipoprotein-cholesterol, and the lowest values of plasma uric acid. The results indicate that melatonin is able to normalize the altered biochemical pro-inflammatory profile seen in rats fed with a high-fat diet.
Fil: Cano Barquilla, Pilar. Universidad Complutense de Madrid; España
Fil: Pagano, Eleonora Samanta. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Ciencias Médicas. Departamento de Docencia e Investigación; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Jiménez Ortega, Vanesa. Universidad Complutense de Madrid; España
Fil: Fernández Mateos, Pilar. Universidad Complutense de Madrid; España
Fil: Esquifino, Ana I.. Universidad Complutense de Madrid; España
Fil: Cardinali, Daniel Pedro. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Ciencias Médicas. Departamento de Docencia e Investigación; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Materia
Cytokines
Dyslipidemia
Glucose Tolerance
High-Fat Diet
Hypertension
Inflammation
Uric Acid
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/30363
Acceder
id CONICETDig_0ac1e01fffffaec2fbeedd12cdf1e310
oai_identifier_str oai:ri.conicet.gov.ar:11336/30363
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Melatonin normalizes clinical and biochemical parameters of mild inflammation in diet-induced metabolic syndrome in ratsCano Barquilla, PilarPagano, Eleonora SamantaJiménez Ortega, VanesaFernández Mateos, PilarEsquifino, Ana I.Cardinali, Daniel PedroCytokinesDyslipidemiaGlucose ToleranceHigh-Fat DietHypertensionInflammationUric Acidhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The objective of this study was to evaluate the efficacy of melatonin to affect mild inflammation in the metabolic syndrome (MS) induced by a high-fat diet in rats. Adult Wistar male rats were divided into four groups (n = 16/group): (i) control diet (3% fat); (ii) high-fat (35%) diet; (iii) high-fat diet + melatonin; and (iv) melatonin. Rats had free access to high-fat or control chow and one of the following drinking solutions for 10 wk: (a) tap water; (b) 25 μg/mL of melatonin. Plasma interleukin (IL)-1β, IL-4, IL-6, IL-10, tumor necrosis factor (TNF)-α, interferon (IFN)-γ, and C-reactive protein (CRP) were measured at two time intervals, that is, the middle of daylight period and the middle of the scotophase. In addition, a number of somatic and metabolic components employed clinically to monitor the MS were measured. Melatonin decreased the augmented circulating levels of IL-1β, IL-6, TNF-α, IFN-γ, and CRP seen in obese rats and restored the depressed levels of IL-4 and IL-10. Rats fed with the high-fat diet showed significantly higher body weights and augmented systolic blood pressure from the third and fourth week onwards, respectively, melatonin effectively preventing these changes. In high-fat-fed rats, circulating low-density lipoprotein-cholesterol, total cholesterol, and triglyceride concentration augmented significantly, melatonin being effective to counteract these changes. Melatonin-treated rats showed a decreased insulin resistance, the highest values of plasma high-density lipoprotein-cholesterol, and the lowest values of plasma uric acid. The results indicate that melatonin is able to normalize the altered biochemical pro-inflammatory profile seen in rats fed with a high-fat diet.Fil: Cano Barquilla, Pilar. Universidad Complutense de Madrid; EspañaFil: Pagano, Eleonora Samanta. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Ciencias Médicas. Departamento de Docencia e Investigación; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Jiménez Ortega, Vanesa. Universidad Complutense de Madrid; EspañaFil: Fernández Mateos, Pilar. Universidad Complutense de Madrid; EspañaFil: Esquifino, Ana I.. Universidad Complutense de Madrid; EspañaFil: Cardinali, Daniel Pedro. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Ciencias Médicas. Departamento de Docencia e Investigación; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaWiley2014-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30363Cano Barquilla, Pilar; Pagano, Eleonora Samanta; Jiménez Ortega, Vanesa; Fernández Mateos, Pilar; Esquifino, Ana I.; et al.; Melatonin normalizes clinical and biochemical parameters of mild inflammation in diet-induced metabolic syndrome in rats; Wiley; Journal of Pineal Research; 57; 3; 9-2014; 280-2900742-30981600-079XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/jpi.12168info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jpi.12168/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:47Zoai:ri.conicet.gov.ar:11336/30363instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:47.632CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Melatonin normalizes clinical and biochemical parameters of mild inflammation in diet-induced metabolic syndrome in rats
title Melatonin normalizes clinical and biochemical parameters of mild inflammation in diet-induced metabolic syndrome in rats
spellingShingle Melatonin normalizes clinical and biochemical parameters of mild inflammation in diet-induced metabolic syndrome in rats
Cano Barquilla, Pilar
Cytokines
Dyslipidemia
Glucose Tolerance
High-Fat Diet
Hypertension
Inflammation
Uric Acid
title_short Melatonin normalizes clinical and biochemical parameters of mild inflammation in diet-induced metabolic syndrome in rats
title_full Melatonin normalizes clinical and biochemical parameters of mild inflammation in diet-induced metabolic syndrome in rats
title_fullStr Melatonin normalizes clinical and biochemical parameters of mild inflammation in diet-induced metabolic syndrome in rats
title_full_unstemmed Melatonin normalizes clinical and biochemical parameters of mild inflammation in diet-induced metabolic syndrome in rats
title_sort Melatonin normalizes clinical and biochemical parameters of mild inflammation in diet-induced metabolic syndrome in rats
dc.creator.none.fl_str_mv Cano Barquilla, Pilar
Pagano, Eleonora Samanta
Jiménez Ortega, Vanesa
Fernández Mateos, Pilar
Esquifino, Ana I.
Cardinali, Daniel Pedro
author Cano Barquilla, Pilar
author_facet Cano Barquilla, Pilar
Pagano, Eleonora Samanta
Jiménez Ortega, Vanesa
Fernández Mateos, Pilar
Esquifino, Ana I.
Cardinali, Daniel Pedro
author_role author
author2 Pagano, Eleonora Samanta
Jiménez Ortega, Vanesa
Fernández Mateos, Pilar
Esquifino, Ana I.
Cardinali, Daniel Pedro
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Cytokines
Dyslipidemia
Glucose Tolerance
High-Fat Diet
Hypertension
Inflammation
Uric Acid
topic Cytokines
Dyslipidemia
Glucose Tolerance
High-Fat Diet
Hypertension
Inflammation
Uric Acid
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The objective of this study was to evaluate the efficacy of melatonin to affect mild inflammation in the metabolic syndrome (MS) induced by a high-fat diet in rats. Adult Wistar male rats were divided into four groups (n = 16/group): (i) control diet (3% fat); (ii) high-fat (35%) diet; (iii) high-fat diet + melatonin; and (iv) melatonin. Rats had free access to high-fat or control chow and one of the following drinking solutions for 10 wk: (a) tap water; (b) 25 μg/mL of melatonin. Plasma interleukin (IL)-1β, IL-4, IL-6, IL-10, tumor necrosis factor (TNF)-α, interferon (IFN)-γ, and C-reactive protein (CRP) were measured at two time intervals, that is, the middle of daylight period and the middle of the scotophase. In addition, a number of somatic and metabolic components employed clinically to monitor the MS were measured. Melatonin decreased the augmented circulating levels of IL-1β, IL-6, TNF-α, IFN-γ, and CRP seen in obese rats and restored the depressed levels of IL-4 and IL-10. Rats fed with the high-fat diet showed significantly higher body weights and augmented systolic blood pressure from the third and fourth week onwards, respectively, melatonin effectively preventing these changes. In high-fat-fed rats, circulating low-density lipoprotein-cholesterol, total cholesterol, and triglyceride concentration augmented significantly, melatonin being effective to counteract these changes. Melatonin-treated rats showed a decreased insulin resistance, the highest values of plasma high-density lipoprotein-cholesterol, and the lowest values of plasma uric acid. The results indicate that melatonin is able to normalize the altered biochemical pro-inflammatory profile seen in rats fed with a high-fat diet.
Fil: Cano Barquilla, Pilar. Universidad Complutense de Madrid; España
Fil: Pagano, Eleonora Samanta. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Ciencias Médicas. Departamento de Docencia e Investigación; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Jiménez Ortega, Vanesa. Universidad Complutense de Madrid; España
Fil: Fernández Mateos, Pilar. Universidad Complutense de Madrid; España
Fil: Esquifino, Ana I.. Universidad Complutense de Madrid; España
Fil: Cardinali, Daniel Pedro. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Ciencias Médicas. Departamento de Docencia e Investigación; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
description The objective of this study was to evaluate the efficacy of melatonin to affect mild inflammation in the metabolic syndrome (MS) induced by a high-fat diet in rats. Adult Wistar male rats were divided into four groups (n = 16/group): (i) control diet (3% fat); (ii) high-fat (35%) diet; (iii) high-fat diet + melatonin; and (iv) melatonin. Rats had free access to high-fat or control chow and one of the following drinking solutions for 10 wk: (a) tap water; (b) 25 μg/mL of melatonin. Plasma interleukin (IL)-1β, IL-4, IL-6, IL-10, tumor necrosis factor (TNF)-α, interferon (IFN)-γ, and C-reactive protein (CRP) were measured at two time intervals, that is, the middle of daylight period and the middle of the scotophase. In addition, a number of somatic and metabolic components employed clinically to monitor the MS were measured. Melatonin decreased the augmented circulating levels of IL-1β, IL-6, TNF-α, IFN-γ, and CRP seen in obese rats and restored the depressed levels of IL-4 and IL-10. Rats fed with the high-fat diet showed significantly higher body weights and augmented systolic blood pressure from the third and fourth week onwards, respectively, melatonin effectively preventing these changes. In high-fat-fed rats, circulating low-density lipoprotein-cholesterol, total cholesterol, and triglyceride concentration augmented significantly, melatonin being effective to counteract these changes. Melatonin-treated rats showed a decreased insulin resistance, the highest values of plasma high-density lipoprotein-cholesterol, and the lowest values of plasma uric acid. The results indicate that melatonin is able to normalize the altered biochemical pro-inflammatory profile seen in rats fed with a high-fat diet.
publishDate 2014
dc.date.none.fl_str_mv 2014-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/30363
Cano Barquilla, Pilar; Pagano, Eleonora Samanta; Jiménez Ortega, Vanesa; Fernández Mateos, Pilar; Esquifino, Ana I.; et al.; Melatonin normalizes clinical and biochemical parameters of mild inflammation in diet-induced metabolic syndrome in rats; Wiley; Journal of Pineal Research; 57; 3; 9-2014; 280-290
0742-3098
1600-079X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/30363
identifier_str_mv Cano Barquilla, Pilar; Pagano, Eleonora Samanta; Jiménez Ortega, Vanesa; Fernández Mateos, Pilar; Esquifino, Ana I.; et al.; Melatonin normalizes clinical and biochemical parameters of mild inflammation in diet-induced metabolic syndrome in rats; Wiley; Journal of Pineal Research; 57; 3; 9-2014; 280-290
0742-3098
1600-079X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/jpi.12168
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jpi.12168/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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