Photodynamic properties and photoinactivation of microorganisms mediated by 5,10,15,20-tetrakis(4-carboxyphenyl)porphyrin covalently linked to silica-coated magnetite nanoparticles

Autores
Scanone, Ana Coral; Gsponer, Natalia Soledad; Alvarez, María Gabriela; Durantini, Edgardo Néstor
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Magnetic nanoparticles of Fe3O4 (MNP) were synthesized by co-precipitating Fe2+ and Fe3+ ions in an ammonia solution. This MNP was coated with silica using sodium metasilicate to obtain silica-coated MNP (MNPSi). Grafting of aminopropyl groups on MNP or MNPSi was performed with (3-aminopropyl)trimethoxysilane to form MNPNH2 or MNPSiNH2, respectively. 5,10,15,20-Tetrakis(4-carboxyphenyl)porphyrin (TCPP) was covalently bound onto the MNPNH2 or MNPSiNH2 via carbodiimide activation to obtain MNPNH-TPCC or MNPNH-TPCC, respectively. These MNP presented an average diameter of about 15 nm. UV–vis absorption spectra presented the characteristic Soret and Q bands of TCPP covalently linked to the nanoparticles. The MNP containing TCPP produced a high photodecomposition of 2,2-(anthracene-9,10-diyl)bis(methylmalonate), which was used to detect singlet molecular oxygen O2(1Δg) production in water. Moreover, these nanoparticles sensitized the photooxidation of L-tryptophan in water, mainly mediated by a type II photoprocess. Photoinactivation of microorganisms was investigated in Staphylococcus aureus, Escherichia coli and Candida albicans. In vitro experiments showed that photosensitized inactivation induced by MNPSiNH-TCPP improved with an increase of irradiation times. After 30 min irradiation, a 2.5 log reduction was found for S. aureus and C. albicans. Also, the last conditions produced a decrease of 3 log in E. coli. Similar result was obtained MNPNH-TCPP. However, the main difference between these nanoparticles as photosensitizer was found after recycling experiments. While the photoinactivation mediated by MNPNH-TCPP rapidly decrease after one cycle, MNPSiNH-TCPP was still efficient after three cycles of inactivation. Therefore, MNPSiNH-TCPP is an interesting and versatile photodynamic active material to eradicate microorganisms.
Fil: Scanone, Ana Coral. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Gsponer, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Alvarez, María Gabriela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Durantini, Edgardo Néstor. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Materia
Magnetic Nanoparticles
Photodynamic Inactivation
Photooxidation
Photosensitizer
Porphyrin
Singlet Oxygen
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/72520
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/72520
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Photodynamic properties and photoinactivation of microorganisms mediated by 5,10,15,20-tetrakis(4-carboxyphenyl)porphyrin covalently linked to silica-coated magnetite nanoparticlesScanone, Ana CoralGsponer, Natalia SoledadAlvarez, María GabrielaDurantini, Edgardo NéstorMagnetic NanoparticlesPhotodynamic InactivationPhotooxidationPhotosensitizerPorphyrinSinglet Oxygenhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Magnetic nanoparticles of Fe3O4 (MNP) were synthesized by co-precipitating Fe2+ and Fe3+ ions in an ammonia solution. This MNP was coated with silica using sodium metasilicate to obtain silica-coated MNP (MNPSi). Grafting of aminopropyl groups on MNP or MNPSi was performed with (3-aminopropyl)trimethoxysilane to form MNPNH2 or MNPSiNH2, respectively. 5,10,15,20-Tetrakis(4-carboxyphenyl)porphyrin (TCPP) was covalently bound onto the MNPNH2 or MNPSiNH2 via carbodiimide activation to obtain MNPNH-TPCC or MNPNH-TPCC, respectively. These MNP presented an average diameter of about 15 nm. UV–vis absorption spectra presented the characteristic Soret and Q bands of TCPP covalently linked to the nanoparticles. The MNP containing TCPP produced a high photodecomposition of 2,2-(anthracene-9,10-diyl)bis(methylmalonate), which was used to detect singlet molecular oxygen O2(1Δg) production in water. Moreover, these nanoparticles sensitized the photooxidation of L-tryptophan in water, mainly mediated by a type II photoprocess. Photoinactivation of microorganisms was investigated in Staphylococcus aureus, Escherichia coli and Candida albicans. In vitro experiments showed that photosensitized inactivation induced by MNPSiNH-TCPP improved with an increase of irradiation times. After 30 min irradiation, a 2.5 log reduction was found for S. aureus and C. albicans. Also, the last conditions produced a decrease of 3 log in E. coli. Similar result was obtained MNPNH-TCPP. However, the main difference between these nanoparticles as photosensitizer was found after recycling experiments. While the photoinactivation mediated by MNPNH-TCPP rapidly decrease after one cycle, MNPSiNH-TCPP was still efficient after three cycles of inactivation. Therefore, MNPSiNH-TCPP is an interesting and versatile photodynamic active material to eradicate microorganisms.Fil: Scanone, Ana Coral. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Gsponer, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Alvarez, María Gabriela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Durantini, Edgardo Néstor. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaElsevier Science Sa2017-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/72520Scanone, Ana Coral; Gsponer, Natalia Soledad; Alvarez, María Gabriela; Durantini, Edgardo Néstor; Photodynamic properties and photoinactivation of microorganisms mediated by 5,10,15,20-tetrakis(4-carboxyphenyl)porphyrin covalently linked to silica-coated magnetite nanoparticles; Elsevier Science Sa; Journal of Photochemistry and Photobiology A: Chemistry; 346; 9-2017; 452-4611010-6030CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jphotochem.2017.06.039info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1010603017301429info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:00Zoai:ri.conicet.gov.ar:11336/72520instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:00.782CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Photodynamic properties and photoinactivation of microorganisms mediated by 5,10,15,20-tetrakis(4-carboxyphenyl)porphyrin covalently linked to silica-coated magnetite nanoparticles
title Photodynamic properties and photoinactivation of microorganisms mediated by 5,10,15,20-tetrakis(4-carboxyphenyl)porphyrin covalently linked to silica-coated magnetite nanoparticles
spellingShingle Photodynamic properties and photoinactivation of microorganisms mediated by 5,10,15,20-tetrakis(4-carboxyphenyl)porphyrin covalently linked to silica-coated magnetite nanoparticles
Scanone, Ana Coral
Magnetic Nanoparticles
Photodynamic Inactivation
Photooxidation
Photosensitizer
Porphyrin
Singlet Oxygen
title_short Photodynamic properties and photoinactivation of microorganisms mediated by 5,10,15,20-tetrakis(4-carboxyphenyl)porphyrin covalently linked to silica-coated magnetite nanoparticles
title_full Photodynamic properties and photoinactivation of microorganisms mediated by 5,10,15,20-tetrakis(4-carboxyphenyl)porphyrin covalently linked to silica-coated magnetite nanoparticles
title_fullStr Photodynamic properties and photoinactivation of microorganisms mediated by 5,10,15,20-tetrakis(4-carboxyphenyl)porphyrin covalently linked to silica-coated magnetite nanoparticles
title_full_unstemmed Photodynamic properties and photoinactivation of microorganisms mediated by 5,10,15,20-tetrakis(4-carboxyphenyl)porphyrin covalently linked to silica-coated magnetite nanoparticles
title_sort Photodynamic properties and photoinactivation of microorganisms mediated by 5,10,15,20-tetrakis(4-carboxyphenyl)porphyrin covalently linked to silica-coated magnetite nanoparticles
dc.creator.none.fl_str_mv Scanone, Ana Coral
Gsponer, Natalia Soledad
Alvarez, María Gabriela
Durantini, Edgardo Néstor
author Scanone, Ana Coral
author_facet Scanone, Ana Coral
Gsponer, Natalia Soledad
Alvarez, María Gabriela
Durantini, Edgardo Néstor
author_role author
author2 Gsponer, Natalia Soledad
Alvarez, María Gabriela
Durantini, Edgardo Néstor
author2_role author
author
author
dc.subject.none.fl_str_mv Magnetic Nanoparticles
Photodynamic Inactivation
Photooxidation
Photosensitizer
Porphyrin
Singlet Oxygen
topic Magnetic Nanoparticles
Photodynamic Inactivation
Photooxidation
Photosensitizer
Porphyrin
Singlet Oxygen
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Magnetic nanoparticles of Fe3O4 (MNP) were synthesized by co-precipitating Fe2+ and Fe3+ ions in an ammonia solution. This MNP was coated with silica using sodium metasilicate to obtain silica-coated MNP (MNPSi). Grafting of aminopropyl groups on MNP or MNPSi was performed with (3-aminopropyl)trimethoxysilane to form MNPNH2 or MNPSiNH2, respectively. 5,10,15,20-Tetrakis(4-carboxyphenyl)porphyrin (TCPP) was covalently bound onto the MNPNH2 or MNPSiNH2 via carbodiimide activation to obtain MNPNH-TPCC or MNPNH-TPCC, respectively. These MNP presented an average diameter of about 15 nm. UV–vis absorption spectra presented the characteristic Soret and Q bands of TCPP covalently linked to the nanoparticles. The MNP containing TCPP produced a high photodecomposition of 2,2-(anthracene-9,10-diyl)bis(methylmalonate), which was used to detect singlet molecular oxygen O2(1Δg) production in water. Moreover, these nanoparticles sensitized the photooxidation of L-tryptophan in water, mainly mediated by a type II photoprocess. Photoinactivation of microorganisms was investigated in Staphylococcus aureus, Escherichia coli and Candida albicans. In vitro experiments showed that photosensitized inactivation induced by MNPSiNH-TCPP improved with an increase of irradiation times. After 30 min irradiation, a 2.5 log reduction was found for S. aureus and C. albicans. Also, the last conditions produced a decrease of 3 log in E. coli. Similar result was obtained MNPNH-TCPP. However, the main difference between these nanoparticles as photosensitizer was found after recycling experiments. While the photoinactivation mediated by MNPNH-TCPP rapidly decrease after one cycle, MNPSiNH-TCPP was still efficient after three cycles of inactivation. Therefore, MNPSiNH-TCPP is an interesting and versatile photodynamic active material to eradicate microorganisms.
Fil: Scanone, Ana Coral. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Gsponer, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Alvarez, María Gabriela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Durantini, Edgardo Néstor. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
description Magnetic nanoparticles of Fe3O4 (MNP) were synthesized by co-precipitating Fe2+ and Fe3+ ions in an ammonia solution. This MNP was coated with silica using sodium metasilicate to obtain silica-coated MNP (MNPSi). Grafting of aminopropyl groups on MNP or MNPSi was performed with (3-aminopropyl)trimethoxysilane to form MNPNH2 or MNPSiNH2, respectively. 5,10,15,20-Tetrakis(4-carboxyphenyl)porphyrin (TCPP) was covalently bound onto the MNPNH2 or MNPSiNH2 via carbodiimide activation to obtain MNPNH-TPCC or MNPNH-TPCC, respectively. These MNP presented an average diameter of about 15 nm. UV–vis absorption spectra presented the characteristic Soret and Q bands of TCPP covalently linked to the nanoparticles. The MNP containing TCPP produced a high photodecomposition of 2,2-(anthracene-9,10-diyl)bis(methylmalonate), which was used to detect singlet molecular oxygen O2(1Δg) production in water. Moreover, these nanoparticles sensitized the photooxidation of L-tryptophan in water, mainly mediated by a type II photoprocess. Photoinactivation of microorganisms was investigated in Staphylococcus aureus, Escherichia coli and Candida albicans. In vitro experiments showed that photosensitized inactivation induced by MNPSiNH-TCPP improved with an increase of irradiation times. After 30 min irradiation, a 2.5 log reduction was found for S. aureus and C. albicans. Also, the last conditions produced a decrease of 3 log in E. coli. Similar result was obtained MNPNH-TCPP. However, the main difference between these nanoparticles as photosensitizer was found after recycling experiments. While the photoinactivation mediated by MNPNH-TCPP rapidly decrease after one cycle, MNPSiNH-TCPP was still efficient after three cycles of inactivation. Therefore, MNPSiNH-TCPP is an interesting and versatile photodynamic active material to eradicate microorganisms.
publishDate 2017
dc.date.none.fl_str_mv 2017-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/72520
Scanone, Ana Coral; Gsponer, Natalia Soledad; Alvarez, María Gabriela; Durantini, Edgardo Néstor; Photodynamic properties and photoinactivation of microorganisms mediated by 5,10,15,20-tetrakis(4-carboxyphenyl)porphyrin covalently linked to silica-coated magnetite nanoparticles; Elsevier Science Sa; Journal of Photochemistry and Photobiology A: Chemistry; 346; 9-2017; 452-461
1010-6030
CONICET Digital
CONICET
url http://hdl.handle.net/11336/72520
identifier_str_mv Scanone, Ana Coral; Gsponer, Natalia Soledad; Alvarez, María Gabriela; Durantini, Edgardo Néstor; Photodynamic properties and photoinactivation of microorganisms mediated by 5,10,15,20-tetrakis(4-carboxyphenyl)porphyrin covalently linked to silica-coated magnetite nanoparticles; Elsevier Science Sa; Journal of Photochemistry and Photobiology A: Chemistry; 346; 9-2017; 452-461
1010-6030
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jphotochem.2017.06.039
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1010603017301429
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Sa
publisher.none.fl_str_mv Elsevier Science Sa
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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