Denitrification-derived nitric oxide modulates biofilm formation in Azospirillum brasilense

Autores
Arruebarrena Di Palma, Andrés; Pereyra, Cintia Mariana; Moreno Ramírez, Lizbeth; Xiqui Vazquez, Maria L.; Baca, Beatriz E.; Pereyra, María Alejandra; Lamattina, Lamattina; Creus, Cecilia Mónica
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Azospirillum brasilense is a rhizobacterium that provides beneficial effects on plants when they colonize roots. The formation of complex bacterial communities known as biofilms begins with the interaction of planktonic cells with surfaces in response to appropriate signals. Nitric oxide (NO) is a signaling molecule implicated in numerous processes in bacteria, including biofilm formation or dispersion, depending on genera and lifestyle. Azospirillum brasilense Sp245 produces NO by denitrification having a role in root growth promotion. We analyzed the role of endogenously produced NO on biofilm formation in A. brasilense Sp245 and in a periplasmic nitrate reductase mutant (napA::Tn5; Faj164) affected in NO production. Cells were statically grown in media with nitrate or ammonium as nitrogen sources and examined for biofilm formation using crystal violet and by confocal laser microscopy. Both strains formed bio- films, but the mutant produced less than half compared with the wild type in nitrate medium showing impaired nitrite production in this condition. NO measurements in biofilm confirmed lower values in the mutant strain. The addition of a NO donor showed that NO influences biofilm formation in a dose-dependent manner and reverses the mutant phenotype, indicating that Nap positively regulates the formation of biofilm in A. brasilense Sp245.
Fil: Arruebarrena Di Palma, Andrés. Universidad Nacional de Mar del Plata; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pereyra, Cintia Mariana. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Moreno Ramírez, Lizbeth. Benemérita Universidad Autónoma de Puebla; México
Fil: Xiqui Vazquez, Maria L.. Benemérita Universidad Autónoma de Puebla; México
Fil: Baca, Beatriz E.. Benemérita Universidad Autónoma de Puebla; México
Fil: Pereyra, María Alejandra. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lamattina, Lamattina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Creus, Cecilia Mónica. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
PLANT-GROWTH-PROMOTING-RHIZOBACTERIA
PERIPLASMIC NITRATE REDUCTASE
NITRIC OXIDE
SIGNALING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/29213
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/29213
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Denitrification-derived nitric oxide modulates biofilm formation in Azospirillum brasilenseArruebarrena Di Palma, AndrésPereyra, Cintia MarianaMoreno Ramírez, LizbethXiqui Vazquez, Maria L.Baca, Beatriz E.Pereyra, María AlejandraLamattina, LamattinaCreus, Cecilia MónicaPLANT-GROWTH-PROMOTING-RHIZOBACTERIAPERIPLASMIC NITRATE REDUCTASENITRIC OXIDESIGNALINGhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Azospirillum brasilense is a rhizobacterium that provides beneficial effects on plants when they colonize roots. The formation of complex bacterial communities known as biofilms begins with the interaction of planktonic cells with surfaces in response to appropriate signals. Nitric oxide (NO) is a signaling molecule implicated in numerous processes in bacteria, including biofilm formation or dispersion, depending on genera and lifestyle. Azospirillum brasilense Sp245 produces NO by denitrification having a role in root growth promotion. We analyzed the role of endogenously produced NO on biofilm formation in A. brasilense Sp245 and in a periplasmic nitrate reductase mutant (napA::Tn5; Faj164) affected in NO production. Cells were statically grown in media with nitrate or ammonium as nitrogen sources and examined for biofilm formation using crystal violet and by confocal laser microscopy. Both strains formed bio- films, but the mutant produced less than half compared with the wild type in nitrate medium showing impaired nitrite production in this condition. NO measurements in biofilm confirmed lower values in the mutant strain. The addition of a NO donor showed that NO influences biofilm formation in a dose-dependent manner and reverses the mutant phenotype, indicating that Nap positively regulates the formation of biofilm in A. brasilense Sp245.Fil: Arruebarrena Di Palma, Andrés. Universidad Nacional de Mar del Plata; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pereyra, Cintia Mariana. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Moreno Ramírez, Lizbeth. Benemérita Universidad Autónoma de Puebla; MéxicoFil: Xiqui Vazquez, Maria L.. Benemérita Universidad Autónoma de Puebla; MéxicoFil: Baca, Beatriz E.. Benemérita Universidad Autónoma de Puebla; MéxicoFil: Pereyra, María Alejandra. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lamattina, Lamattina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Creus, Cecilia Mónica. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley Blackwell Publishing, Inc2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/29213Arruebarrena Di Palma, Andrés; Pereyra, Cintia Mariana; Moreno Ramírez, Lizbeth; Xiqui Vazquez, Maria L.; Baca, Beatriz E.; et al.; Denitrification-derived nitric oxide modulates biofilm formation in Azospirillum brasilense; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 338; 1; 1-2013; 77-850378-1097CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://femsle.oxfordjournals.org/content/338/1/77info:eu-repo/semantics/altIdentifier/doi/10.1111/1574-6968.12030info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:14:41Zoai:ri.conicet.gov.ar:11336/29213instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:14:42.097CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Denitrification-derived nitric oxide modulates biofilm formation in Azospirillum brasilense
title Denitrification-derived nitric oxide modulates biofilm formation in Azospirillum brasilense
spellingShingle Denitrification-derived nitric oxide modulates biofilm formation in Azospirillum brasilense
Arruebarrena Di Palma, Andrés
PLANT-GROWTH-PROMOTING-RHIZOBACTERIA
PERIPLASMIC NITRATE REDUCTASE
NITRIC OXIDE
SIGNALING
title_short Denitrification-derived nitric oxide modulates biofilm formation in Azospirillum brasilense
title_full Denitrification-derived nitric oxide modulates biofilm formation in Azospirillum brasilense
title_fullStr Denitrification-derived nitric oxide modulates biofilm formation in Azospirillum brasilense
title_full_unstemmed Denitrification-derived nitric oxide modulates biofilm formation in Azospirillum brasilense
title_sort Denitrification-derived nitric oxide modulates biofilm formation in Azospirillum brasilense
dc.creator.none.fl_str_mv Arruebarrena Di Palma, Andrés
Pereyra, Cintia Mariana
Moreno Ramírez, Lizbeth
Xiqui Vazquez, Maria L.
Baca, Beatriz E.
Pereyra, María Alejandra
Lamattina, Lamattina
Creus, Cecilia Mónica
author Arruebarrena Di Palma, Andrés
author_facet Arruebarrena Di Palma, Andrés
Pereyra, Cintia Mariana
Moreno Ramírez, Lizbeth
Xiqui Vazquez, Maria L.
Baca, Beatriz E.
Pereyra, María Alejandra
Lamattina, Lamattina
Creus, Cecilia Mónica
author_role author
author2 Pereyra, Cintia Mariana
Moreno Ramírez, Lizbeth
Xiqui Vazquez, Maria L.
Baca, Beatriz E.
Pereyra, María Alejandra
Lamattina, Lamattina
Creus, Cecilia Mónica
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PLANT-GROWTH-PROMOTING-RHIZOBACTERIA
PERIPLASMIC NITRATE REDUCTASE
NITRIC OXIDE
SIGNALING
topic PLANT-GROWTH-PROMOTING-RHIZOBACTERIA
PERIPLASMIC NITRATE REDUCTASE
NITRIC OXIDE
SIGNALING
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Azospirillum brasilense is a rhizobacterium that provides beneficial effects on plants when they colonize roots. The formation of complex bacterial communities known as biofilms begins with the interaction of planktonic cells with surfaces in response to appropriate signals. Nitric oxide (NO) is a signaling molecule implicated in numerous processes in bacteria, including biofilm formation or dispersion, depending on genera and lifestyle. Azospirillum brasilense Sp245 produces NO by denitrification having a role in root growth promotion. We analyzed the role of endogenously produced NO on biofilm formation in A. brasilense Sp245 and in a periplasmic nitrate reductase mutant (napA::Tn5; Faj164) affected in NO production. Cells were statically grown in media with nitrate or ammonium as nitrogen sources and examined for biofilm formation using crystal violet and by confocal laser microscopy. Both strains formed bio- films, but the mutant produced less than half compared with the wild type in nitrate medium showing impaired nitrite production in this condition. NO measurements in biofilm confirmed lower values in the mutant strain. The addition of a NO donor showed that NO influences biofilm formation in a dose-dependent manner and reverses the mutant phenotype, indicating that Nap positively regulates the formation of biofilm in A. brasilense Sp245.
Fil: Arruebarrena Di Palma, Andrés. Universidad Nacional de Mar del Plata; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pereyra, Cintia Mariana. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Moreno Ramírez, Lizbeth. Benemérita Universidad Autónoma de Puebla; México
Fil: Xiqui Vazquez, Maria L.. Benemérita Universidad Autónoma de Puebla; México
Fil: Baca, Beatriz E.. Benemérita Universidad Autónoma de Puebla; México
Fil: Pereyra, María Alejandra. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lamattina, Lamattina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Creus, Cecilia Mónica. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Azospirillum brasilense is a rhizobacterium that provides beneficial effects on plants when they colonize roots. The formation of complex bacterial communities known as biofilms begins with the interaction of planktonic cells with surfaces in response to appropriate signals. Nitric oxide (NO) is a signaling molecule implicated in numerous processes in bacteria, including biofilm formation or dispersion, depending on genera and lifestyle. Azospirillum brasilense Sp245 produces NO by denitrification having a role in root growth promotion. We analyzed the role of endogenously produced NO on biofilm formation in A. brasilense Sp245 and in a periplasmic nitrate reductase mutant (napA::Tn5; Faj164) affected in NO production. Cells were statically grown in media with nitrate or ammonium as nitrogen sources and examined for biofilm formation using crystal violet and by confocal laser microscopy. Both strains formed bio- films, but the mutant produced less than half compared with the wild type in nitrate medium showing impaired nitrite production in this condition. NO measurements in biofilm confirmed lower values in the mutant strain. The addition of a NO donor showed that NO influences biofilm formation in a dose-dependent manner and reverses the mutant phenotype, indicating that Nap positively regulates the formation of biofilm in A. brasilense Sp245.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/29213
Arruebarrena Di Palma, Andrés; Pereyra, Cintia Mariana; Moreno Ramírez, Lizbeth; Xiqui Vazquez, Maria L.; Baca, Beatriz E.; et al.; Denitrification-derived nitric oxide modulates biofilm formation in Azospirillum brasilense; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 338; 1; 1-2013; 77-85
0378-1097
CONICET Digital
CONICET
url http://hdl.handle.net/11336/29213
identifier_str_mv Arruebarrena Di Palma, Andrés; Pereyra, Cintia Mariana; Moreno Ramírez, Lizbeth; Xiqui Vazquez, Maria L.; Baca, Beatriz E.; et al.; Denitrification-derived nitric oxide modulates biofilm formation in Azospirillum brasilense; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 338; 1; 1-2013; 77-85
0378-1097
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://femsle.oxfordjournals.org/content/338/1/77
info:eu-repo/semantics/altIdentifier/doi/10.1111/1574-6968.12030
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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