Spectroscopy, microscopy and fluorescence imaging of origanum vulgare L. Basis for nondestructive quality assessment

Autores
Johanna Mendes Novo; Iriel, Analia; Marchi, María Claudia; Lagorio, María Gabriela
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The organs of Origanum vulgare L. plant were examined by optical microscopy, scanning electron microscopy and autofluorescence imaging. The different organs were also studied spectroscopically. Fluorescence emission spectra were recorded for intact inflorescences, leaves and stems. Several fluorescence ratios (Blue/Red, Blue/Far-red, Green/Red and Green/Far-red), which varied depending on the considered organ of the plant, were derived. For leaves, a dependence of fluorescence spectra with water content was obtained as well. The intact samples were also analyzed by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy. These spectra were transformed to the Remission function depending on the wavenumber and two absorption bands (811 and 1740 cm-1), which displayed differences according to the plant organ sampled, were detected. These results were consistent with higher carvacrol content in inflorescences. The spectroscopic results were connected with the microscopic observation and with the presence of relevant nutraceutics contained in the plant. The optical indexes derived in this work may serve as potential indicators to be explored in the development of nondestructive methods for oregano quality assessment. © 2013 The American Society of Photobiology.
Fil: Johanna Mendes Novo. Universidad de Buenos Aires; Argentina
Fil: Iriel, Analia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Marchi, María Claudia. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Química Inorganica, Anal.y Qca.física. Area de Fotoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Lagorio, María Gabriela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Materia
Non Destructive Methods
Biospectroscopy
Oeiganum Vulgare
Fluorescence
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/77177
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/77177
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Spectroscopy, microscopy and fluorescence imaging of origanum vulgare L. Basis for nondestructive quality assessmentJohanna Mendes NovoIriel, AnaliaMarchi, María ClaudiaLagorio, María GabrielaNon Destructive MethodsBiospectroscopyOeiganum VulgareFluorescencehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The organs of Origanum vulgare L. plant were examined by optical microscopy, scanning electron microscopy and autofluorescence imaging. The different organs were also studied spectroscopically. Fluorescence emission spectra were recorded for intact inflorescences, leaves and stems. Several fluorescence ratios (Blue/Red, Blue/Far-red, Green/Red and Green/Far-red), which varied depending on the considered organ of the plant, were derived. For leaves, a dependence of fluorescence spectra with water content was obtained as well. The intact samples were also analyzed by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy. These spectra were transformed to the Remission function depending on the wavenumber and two absorption bands (811 and 1740 cm-1), which displayed differences according to the plant organ sampled, were detected. These results were consistent with higher carvacrol content in inflorescences. The spectroscopic results were connected with the microscopic observation and with the presence of relevant nutraceutics contained in the plant. The optical indexes derived in this work may serve as potential indicators to be explored in the development of nondestructive methods for oregano quality assessment. © 2013 The American Society of Photobiology.Fil: Johanna Mendes Novo. Universidad de Buenos Aires; ArgentinaFil: Iriel, Analia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaFil: Marchi, María Claudia. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Química Inorganica, Anal.y Qca.física. Area de Fotoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaFil: Lagorio, María Gabriela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaWiley Blackwell Publishing, Inc2013-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/77177Johanna Mendes Novo; Iriel, Analia; Marchi, María Claudia; Lagorio, María Gabriela; Spectroscopy, microscopy and fluorescence imaging of origanum vulgare L. Basis for nondestructive quality assessment; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 89; 6; 7-2013; 1383-13900031-8655CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/php.12131/abstract/info:eu-repo/semantics/altIdentifier/doi/10.1111/php.12131info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:47:29Zoai:ri.conicet.gov.ar:11336/77177instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:47:29.953CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Spectroscopy, microscopy and fluorescence imaging of origanum vulgare L. Basis for nondestructive quality assessment
title Spectroscopy, microscopy and fluorescence imaging of origanum vulgare L. Basis for nondestructive quality assessment
spellingShingle Spectroscopy, microscopy and fluorescence imaging of origanum vulgare L. Basis for nondestructive quality assessment
Johanna Mendes Novo
Non Destructive Methods
Biospectroscopy
Oeiganum Vulgare
Fluorescence
title_short Spectroscopy, microscopy and fluorescence imaging of origanum vulgare L. Basis for nondestructive quality assessment
title_full Spectroscopy, microscopy and fluorescence imaging of origanum vulgare L. Basis for nondestructive quality assessment
title_fullStr Spectroscopy, microscopy and fluorescence imaging of origanum vulgare L. Basis for nondestructive quality assessment
title_full_unstemmed Spectroscopy, microscopy and fluorescence imaging of origanum vulgare L. Basis for nondestructive quality assessment
title_sort Spectroscopy, microscopy and fluorescence imaging of origanum vulgare L. Basis for nondestructive quality assessment
dc.creator.none.fl_str_mv Johanna Mendes Novo
Iriel, Analia
Marchi, María Claudia
Lagorio, María Gabriela
author Johanna Mendes Novo
author_facet Johanna Mendes Novo
Iriel, Analia
Marchi, María Claudia
Lagorio, María Gabriela
author_role author
author2 Iriel, Analia
Marchi, María Claudia
Lagorio, María Gabriela
author2_role author
author
author
dc.subject.none.fl_str_mv Non Destructive Methods
Biospectroscopy
Oeiganum Vulgare
Fluorescence
topic Non Destructive Methods
Biospectroscopy
Oeiganum Vulgare
Fluorescence
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The organs of Origanum vulgare L. plant were examined by optical microscopy, scanning electron microscopy and autofluorescence imaging. The different organs were also studied spectroscopically. Fluorescence emission spectra were recorded for intact inflorescences, leaves and stems. Several fluorescence ratios (Blue/Red, Blue/Far-red, Green/Red and Green/Far-red), which varied depending on the considered organ of the plant, were derived. For leaves, a dependence of fluorescence spectra with water content was obtained as well. The intact samples were also analyzed by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy. These spectra were transformed to the Remission function depending on the wavenumber and two absorption bands (811 and 1740 cm-1), which displayed differences according to the plant organ sampled, were detected. These results were consistent with higher carvacrol content in inflorescences. The spectroscopic results were connected with the microscopic observation and with the presence of relevant nutraceutics contained in the plant. The optical indexes derived in this work may serve as potential indicators to be explored in the development of nondestructive methods for oregano quality assessment. © 2013 The American Society of Photobiology.
Fil: Johanna Mendes Novo. Universidad de Buenos Aires; Argentina
Fil: Iriel, Analia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Marchi, María Claudia. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Química Inorganica, Anal.y Qca.física. Area de Fotoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Lagorio, María Gabriela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
description The organs of Origanum vulgare L. plant were examined by optical microscopy, scanning electron microscopy and autofluorescence imaging. The different organs were also studied spectroscopically. Fluorescence emission spectra were recorded for intact inflorescences, leaves and stems. Several fluorescence ratios (Blue/Red, Blue/Far-red, Green/Red and Green/Far-red), which varied depending on the considered organ of the plant, were derived. For leaves, a dependence of fluorescence spectra with water content was obtained as well. The intact samples were also analyzed by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy. These spectra were transformed to the Remission function depending on the wavenumber and two absorption bands (811 and 1740 cm-1), which displayed differences according to the plant organ sampled, were detected. These results were consistent with higher carvacrol content in inflorescences. The spectroscopic results were connected with the microscopic observation and with the presence of relevant nutraceutics contained in the plant. The optical indexes derived in this work may serve as potential indicators to be explored in the development of nondestructive methods for oregano quality assessment. © 2013 The American Society of Photobiology.
publishDate 2013
dc.date.none.fl_str_mv 2013-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/77177
Johanna Mendes Novo; Iriel, Analia; Marchi, María Claudia; Lagorio, María Gabriela; Spectroscopy, microscopy and fluorescence imaging of origanum vulgare L. Basis for nondestructive quality assessment; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 89; 6; 7-2013; 1383-1390
0031-8655
CONICET Digital
CONICET
url http://hdl.handle.net/11336/77177
identifier_str_mv Johanna Mendes Novo; Iriel, Analia; Marchi, María Claudia; Lagorio, María Gabriela; Spectroscopy, microscopy and fluorescence imaging of origanum vulgare L. Basis for nondestructive quality assessment; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 89; 6; 7-2013; 1383-1390
0031-8655
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/php.12131/abstract/
info:eu-repo/semantics/altIdentifier/doi/10.1111/php.12131
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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