P2X7 receptor is essential for cross-dressing of bone marrow-derived dendritic cells

Autores
Barrera Avalos, Carlos; Briceño, Pedro; Valdés, Daniel; Imarai, Mónica; Leiva Salcedo, Elías; Rojo, Leonel E.; Milla, Luis A.; Huidobro Toro, Juan Pablo; Robles Planells, Claudia; Escobar, Alejandro; Di Virgilio, Francesco; Moron, Victor Gabriel; Sauma, Daniela; Acuña Castillo, Claudio
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
T cell activation requires the processing and presentation of antigenic peptides in the context of a major histocompatibility complex (MHC complex). Cross-dressing is a non-conventional antigen presentation mechanism, involving the transfer of preformed peptide/MHC complexes from whole cells, such as apoptotic cells (ACs) to the cell membrane of professional antigen-presenting cells (APCs), such as dendritic cells (DCs). This is an essential mechanism for the induction of immune response against viral antigens, tumors, and graft rejection, which until now has not been clarified. Here we show for first time that the P2X7 receptor (P2X7R) is crucial to induce cross-dressing between ACs and Bone-Marrow DCs (BMDCs). In controlled ex vivo assays, we found that the P2X7R in both ACs and BMDCs is required to induce membrane and fully functional peptide/MHC complex transfer to BMDCs. These findings show that acquisition of ACs-derived preformed antigen/MHC-I complexes by BMDCs requires P2X7R expression.
Fil: Barrera Avalos, Carlos. Universidad de Santiago de Chile; Chile
Fil: Briceño, Pedro. Universidad de Chile; Chile
Fil: Valdés, Daniel. Universidad de Santiago de Chile; Chile
Fil: Imarai, Mónica. Universidad de Santiago de Chile; Chile
Fil: Leiva Salcedo, Elías. Universidad de Santiago de Chile; Chile
Fil: Rojo, Leonel E.. Universidad de Santiago de Chile; Chile
Fil: Milla, Luis A.. Universidad de Santiago de Chile; Chile
Fil: Huidobro Toro, Juan Pablo. Universidad de Santiago de Chile; Chile
Fil: Robles Planells, Claudia. Universidad de Santiago de Chile; Chile
Fil: Escobar, Alejandro. Universidad de Chile; Chile
Fil: Di Virgilio, Francesco. Università di Ferrara; Italia
Fil: Moron, Victor Gabriel. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Sauma, Daniela. Universidad de Chile; Chile
Fil: Acuña Castillo, Claudio. Universidad de Santiago de Chile; Chile
Materia
CELL BIOLOGY
IMMUNE RESPONSE
IMMUNE SYSTEM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/184734
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/184734
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling P2X7 receptor is essential for cross-dressing of bone marrow-derived dendritic cellsBarrera Avalos, CarlosBriceño, PedroValdés, DanielImarai, MónicaLeiva Salcedo, ElíasRojo, Leonel E.Milla, Luis A.Huidobro Toro, Juan PabloRobles Planells, ClaudiaEscobar, AlejandroDi Virgilio, FrancescoMoron, Victor GabrielSauma, DanielaAcuña Castillo, ClaudioCELL BIOLOGYIMMUNE RESPONSEIMMUNE SYSTEMhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3T cell activation requires the processing and presentation of antigenic peptides in the context of a major histocompatibility complex (MHC complex). Cross-dressing is a non-conventional antigen presentation mechanism, involving the transfer of preformed peptide/MHC complexes from whole cells, such as apoptotic cells (ACs) to the cell membrane of professional antigen-presenting cells (APCs), such as dendritic cells (DCs). This is an essential mechanism for the induction of immune response against viral antigens, tumors, and graft rejection, which until now has not been clarified. Here we show for first time that the P2X7 receptor (P2X7R) is crucial to induce cross-dressing between ACs and Bone-Marrow DCs (BMDCs). In controlled ex vivo assays, we found that the P2X7R in both ACs and BMDCs is required to induce membrane and fully functional peptide/MHC complex transfer to BMDCs. These findings show that acquisition of ACs-derived preformed antigen/MHC-I complexes by BMDCs requires P2X7R expression.Fil: Barrera Avalos, Carlos. Universidad de Santiago de Chile; ChileFil: Briceño, Pedro. Universidad de Chile; ChileFil: Valdés, Daniel. Universidad de Santiago de Chile; ChileFil: Imarai, Mónica. Universidad de Santiago de Chile; ChileFil: Leiva Salcedo, Elías. Universidad de Santiago de Chile; ChileFil: Rojo, Leonel E.. Universidad de Santiago de Chile; ChileFil: Milla, Luis A.. Universidad de Santiago de Chile; ChileFil: Huidobro Toro, Juan Pablo. Universidad de Santiago de Chile; ChileFil: Robles Planells, Claudia. Universidad de Santiago de Chile; ChileFil: Escobar, Alejandro. Universidad de Chile; ChileFil: Di Virgilio, Francesco. Università di Ferrara; ItaliaFil: Moron, Victor Gabriel. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Sauma, Daniela. Universidad de Chile; ChileFil: Acuña Castillo, Claudio. Universidad de Santiago de Chile; ChileElsevier Inc.2021-11-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/184734Barrera Avalos, Carlos; Briceño, Pedro; Valdés, Daniel; Imarai, Mónica; Leiva Salcedo, Elías; et al.; P2X7 receptor is essential for cross-dressing of bone marrow-derived dendritic cells; Elsevier Inc.; iScience; 24; 12; 26-11-2021; 1-192589-0042CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.isci.2021.103520info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:03:01Zoai:ri.conicet.gov.ar:11336/184734instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:03:01.912CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv P2X7 receptor is essential for cross-dressing of bone marrow-derived dendritic cells
title P2X7 receptor is essential for cross-dressing of bone marrow-derived dendritic cells
spellingShingle P2X7 receptor is essential for cross-dressing of bone marrow-derived dendritic cells
Barrera Avalos, Carlos
CELL BIOLOGY
IMMUNE RESPONSE
IMMUNE SYSTEM
title_short P2X7 receptor is essential for cross-dressing of bone marrow-derived dendritic cells
title_full P2X7 receptor is essential for cross-dressing of bone marrow-derived dendritic cells
title_fullStr P2X7 receptor is essential for cross-dressing of bone marrow-derived dendritic cells
title_full_unstemmed P2X7 receptor is essential for cross-dressing of bone marrow-derived dendritic cells
title_sort P2X7 receptor is essential for cross-dressing of bone marrow-derived dendritic cells
dc.creator.none.fl_str_mv Barrera Avalos, Carlos
Briceño, Pedro
Valdés, Daniel
Imarai, Mónica
Leiva Salcedo, Elías
Rojo, Leonel E.
Milla, Luis A.
Huidobro Toro, Juan Pablo
Robles Planells, Claudia
Escobar, Alejandro
Di Virgilio, Francesco
Moron, Victor Gabriel
Sauma, Daniela
Acuña Castillo, Claudio
author Barrera Avalos, Carlos
author_facet Barrera Avalos, Carlos
Briceño, Pedro
Valdés, Daniel
Imarai, Mónica
Leiva Salcedo, Elías
Rojo, Leonel E.
Milla, Luis A.
Huidobro Toro, Juan Pablo
Robles Planells, Claudia
Escobar, Alejandro
Di Virgilio, Francesco
Moron, Victor Gabriel
Sauma, Daniela
Acuña Castillo, Claudio
author_role author
author2 Briceño, Pedro
Valdés, Daniel
Imarai, Mónica
Leiva Salcedo, Elías
Rojo, Leonel E.
Milla, Luis A.
Huidobro Toro, Juan Pablo
Robles Planells, Claudia
Escobar, Alejandro
Di Virgilio, Francesco
Moron, Victor Gabriel
Sauma, Daniela
Acuña Castillo, Claudio
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CELL BIOLOGY
IMMUNE RESPONSE
IMMUNE SYSTEM
topic CELL BIOLOGY
IMMUNE RESPONSE
IMMUNE SYSTEM
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv T cell activation requires the processing and presentation of antigenic peptides in the context of a major histocompatibility complex (MHC complex). Cross-dressing is a non-conventional antigen presentation mechanism, involving the transfer of preformed peptide/MHC complexes from whole cells, such as apoptotic cells (ACs) to the cell membrane of professional antigen-presenting cells (APCs), such as dendritic cells (DCs). This is an essential mechanism for the induction of immune response against viral antigens, tumors, and graft rejection, which until now has not been clarified. Here we show for first time that the P2X7 receptor (P2X7R) is crucial to induce cross-dressing between ACs and Bone-Marrow DCs (BMDCs). In controlled ex vivo assays, we found that the P2X7R in both ACs and BMDCs is required to induce membrane and fully functional peptide/MHC complex transfer to BMDCs. These findings show that acquisition of ACs-derived preformed antigen/MHC-I complexes by BMDCs requires P2X7R expression.
Fil: Barrera Avalos, Carlos. Universidad de Santiago de Chile; Chile
Fil: Briceño, Pedro. Universidad de Chile; Chile
Fil: Valdés, Daniel. Universidad de Santiago de Chile; Chile
Fil: Imarai, Mónica. Universidad de Santiago de Chile; Chile
Fil: Leiva Salcedo, Elías. Universidad de Santiago de Chile; Chile
Fil: Rojo, Leonel E.. Universidad de Santiago de Chile; Chile
Fil: Milla, Luis A.. Universidad de Santiago de Chile; Chile
Fil: Huidobro Toro, Juan Pablo. Universidad de Santiago de Chile; Chile
Fil: Robles Planells, Claudia. Universidad de Santiago de Chile; Chile
Fil: Escobar, Alejandro. Universidad de Chile; Chile
Fil: Di Virgilio, Francesco. Università di Ferrara; Italia
Fil: Moron, Victor Gabriel. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Sauma, Daniela. Universidad de Chile; Chile
Fil: Acuña Castillo, Claudio. Universidad de Santiago de Chile; Chile
description T cell activation requires the processing and presentation of antigenic peptides in the context of a major histocompatibility complex (MHC complex). Cross-dressing is a non-conventional antigen presentation mechanism, involving the transfer of preformed peptide/MHC complexes from whole cells, such as apoptotic cells (ACs) to the cell membrane of professional antigen-presenting cells (APCs), such as dendritic cells (DCs). This is an essential mechanism for the induction of immune response against viral antigens, tumors, and graft rejection, which until now has not been clarified. Here we show for first time that the P2X7 receptor (P2X7R) is crucial to induce cross-dressing between ACs and Bone-Marrow DCs (BMDCs). In controlled ex vivo assays, we found that the P2X7R in both ACs and BMDCs is required to induce membrane and fully functional peptide/MHC complex transfer to BMDCs. These findings show that acquisition of ACs-derived preformed antigen/MHC-I complexes by BMDCs requires P2X7R expression.
publishDate 2021
dc.date.none.fl_str_mv 2021-11-26
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/184734
Barrera Avalos, Carlos; Briceño, Pedro; Valdés, Daniel; Imarai, Mónica; Leiva Salcedo, Elías; et al.; P2X7 receptor is essential for cross-dressing of bone marrow-derived dendritic cells; Elsevier Inc.; iScience; 24; 12; 26-11-2021; 1-19
2589-0042
CONICET Digital
CONICET
url http://hdl.handle.net/11336/184734
identifier_str_mv Barrera Avalos, Carlos; Briceño, Pedro; Valdés, Daniel; Imarai, Mónica; Leiva Salcedo, Elías; et al.; P2X7 receptor is essential for cross-dressing of bone marrow-derived dendritic cells; Elsevier Inc.; iScience; 24; 12; 26-11-2021; 1-19
2589-0042
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.isci.2021.103520
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Inc.
publisher.none.fl_str_mv Elsevier Inc.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083172639440896
score 13.22299

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