Lipopolysaccharide O-chain core region required for cellular cohesion and compaction of In Vitro and root biofilms developed by Rhizobium leguminosarum

Autores
Russo, Daniela Marta; Abdian, Patricia Lorena; Posadas, Diana Maria; Williams, Alan; Vozza, Nicolas Federico; Giordano, Walter Fabian; Kannenberg, Elmar; Allan Downie, J.; Zorreguietaa, Angeles
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The formation of biofilms is an important survival strategy allowing rhizobia to live on soil particles and plant roots. Within the microcolonies of the biofilm developed by Rhizobium leguminosarum, rhizobial cells interact tightly through lateral and polar connections, forming organized and compact cell aggregates. These microcolonies are embedded in a biofilm matrix, whose main component is the acidic exopolysaccharide (EPS). Our work shows that the O-chain core region of the R. leguminosarum lipopolysaccharide (LPS) (which stretches out of the cell surface) strongly influences bacterial adhesive properties and cell-cell cohesion. Mutants defective in the O chain or O-chain core moiety developed premature microcolonies in which lateral bacterial contacts were greatly reduced. Furthermore, cell-cell interactions within the microcolonies of the LPS mutants were mediated mostly through their poles, resulting in a biofilm with an altered three-dimensional structure and increased thickness. In addition, on the root epidermis and on root hairs, O-antigen core-defective strains showed altered biofilm patterns with the typical microcolony compaction impaired. Taken together, these results indicate that the surface-exposed moiety of the LPS is crucial for proper cell-to-cell interactions and for the formation of robust biofilms on different surfaces.
Fil: Russo, Daniela Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Abdian, Patricia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Posadas, Diana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Williams, Alan. John Innes Institute; Reino Unido
Fil: Vozza, Nicolas Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Giordano, Walter Fabian. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kannenberg, Elmar. University of Georgia; Estados Unidos
Fil: Allan Downie, J.. John Innes Institute; Reino Unido
Fil: Zorreguietaa, Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Materia
RHIZOBIUM
BIOFILM
LIPOPOLYSACCHARIDE
CELLULAR INTERACTIONS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/38568
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/38568
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lipopolysaccharide O-chain core region required for cellular cohesion and compaction of In Vitro and root biofilms developed by Rhizobium leguminosarumRusso, Daniela MartaAbdian, Patricia LorenaPosadas, Diana MariaWilliams, AlanVozza, Nicolas FedericoGiordano, Walter FabianKannenberg, ElmarAllan Downie, J.Zorreguietaa, AngelesRHIZOBIUMBIOFILMLIPOPOLYSACCHARIDECELLULAR INTERACTIONShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The formation of biofilms is an important survival strategy allowing rhizobia to live on soil particles and plant roots. Within the microcolonies of the biofilm developed by Rhizobium leguminosarum, rhizobial cells interact tightly through lateral and polar connections, forming organized and compact cell aggregates. These microcolonies are embedded in a biofilm matrix, whose main component is the acidic exopolysaccharide (EPS). Our work shows that the O-chain core region of the R. leguminosarum lipopolysaccharide (LPS) (which stretches out of the cell surface) strongly influences bacterial adhesive properties and cell-cell cohesion. Mutants defective in the O chain or O-chain core moiety developed premature microcolonies in which lateral bacterial contacts were greatly reduced. Furthermore, cell-cell interactions within the microcolonies of the LPS mutants were mediated mostly through their poles, resulting in a biofilm with an altered three-dimensional structure and increased thickness. In addition, on the root epidermis and on root hairs, O-antigen core-defective strains showed altered biofilm patterns with the typical microcolony compaction impaired. Taken together, these results indicate that the surface-exposed moiety of the LPS is crucial for proper cell-to-cell interactions and for the formation of robust biofilms on different surfaces.Fil: Russo, Daniela Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Abdian, Patricia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Posadas, Diana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Williams, Alan. John Innes Institute; Reino UnidoFil: Vozza, Nicolas Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Giordano, Walter Fabian. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Kannenberg, Elmar. University of Georgia; Estados UnidosFil: Allan Downie, J.. John Innes Institute; Reino UnidoFil: Zorreguietaa, Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaAmerican Society for Microbiology2015-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/38568Russo, Daniela Marta; Abdian, Patricia Lorena; Posadas, Diana Maria; Williams, Alan; Vozza, Nicolas Federico; et al.; Lipopolysaccharide O-chain core region required for cellular cohesion and compaction of In Vitro and root biofilms developed by Rhizobium leguminosarum; American Society for Microbiology; Applied And Environmental Microbiology; 81; 3; 2-2015; 1013-10230099-2240CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1128/AEM.03175-14info:eu-repo/semantics/altIdentifier/url/http://aem.asm.org/content/early/2014/11/17/AEM.03175-14info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4292486/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:35:36Zoai:ri.conicet.gov.ar:11336/38568instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:35:36.305CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lipopolysaccharide O-chain core region required for cellular cohesion and compaction of In Vitro and root biofilms developed by Rhizobium leguminosarum
title Lipopolysaccharide O-chain core region required for cellular cohesion and compaction of In Vitro and root biofilms developed by Rhizobium leguminosarum
spellingShingle Lipopolysaccharide O-chain core region required for cellular cohesion and compaction of In Vitro and root biofilms developed by Rhizobium leguminosarum
Russo, Daniela Marta
RHIZOBIUM
BIOFILM
LIPOPOLYSACCHARIDE
CELLULAR INTERACTIONS
title_short Lipopolysaccharide O-chain core region required for cellular cohesion and compaction of In Vitro and root biofilms developed by Rhizobium leguminosarum
title_full Lipopolysaccharide O-chain core region required for cellular cohesion and compaction of In Vitro and root biofilms developed by Rhizobium leguminosarum
title_fullStr Lipopolysaccharide O-chain core region required for cellular cohesion and compaction of In Vitro and root biofilms developed by Rhizobium leguminosarum
title_full_unstemmed Lipopolysaccharide O-chain core region required for cellular cohesion and compaction of In Vitro and root biofilms developed by Rhizobium leguminosarum
title_sort Lipopolysaccharide O-chain core region required for cellular cohesion and compaction of In Vitro and root biofilms developed by Rhizobium leguminosarum
dc.creator.none.fl_str_mv Russo, Daniela Marta
Abdian, Patricia Lorena
Posadas, Diana Maria
Williams, Alan
Vozza, Nicolas Federico
Giordano, Walter Fabian
Kannenberg, Elmar
Allan Downie, J.
Zorreguietaa, Angeles
author Russo, Daniela Marta
author_facet Russo, Daniela Marta
Abdian, Patricia Lorena
Posadas, Diana Maria
Williams, Alan
Vozza, Nicolas Federico
Giordano, Walter Fabian
Kannenberg, Elmar
Allan Downie, J.
Zorreguietaa, Angeles
author_role author
author2 Abdian, Patricia Lorena
Posadas, Diana Maria
Williams, Alan
Vozza, Nicolas Federico
Giordano, Walter Fabian
Kannenberg, Elmar
Allan Downie, J.
Zorreguietaa, Angeles
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv RHIZOBIUM
BIOFILM
LIPOPOLYSACCHARIDE
CELLULAR INTERACTIONS
topic RHIZOBIUM
BIOFILM
LIPOPOLYSACCHARIDE
CELLULAR INTERACTIONS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The formation of biofilms is an important survival strategy allowing rhizobia to live on soil particles and plant roots. Within the microcolonies of the biofilm developed by Rhizobium leguminosarum, rhizobial cells interact tightly through lateral and polar connections, forming organized and compact cell aggregates. These microcolonies are embedded in a biofilm matrix, whose main component is the acidic exopolysaccharide (EPS). Our work shows that the O-chain core region of the R. leguminosarum lipopolysaccharide (LPS) (which stretches out of the cell surface) strongly influences bacterial adhesive properties and cell-cell cohesion. Mutants defective in the O chain or O-chain core moiety developed premature microcolonies in which lateral bacterial contacts were greatly reduced. Furthermore, cell-cell interactions within the microcolonies of the LPS mutants were mediated mostly through their poles, resulting in a biofilm with an altered three-dimensional structure and increased thickness. In addition, on the root epidermis and on root hairs, O-antigen core-defective strains showed altered biofilm patterns with the typical microcolony compaction impaired. Taken together, these results indicate that the surface-exposed moiety of the LPS is crucial for proper cell-to-cell interactions and for the formation of robust biofilms on different surfaces.
Fil: Russo, Daniela Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Abdian, Patricia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Posadas, Diana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Williams, Alan. John Innes Institute; Reino Unido
Fil: Vozza, Nicolas Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Giordano, Walter Fabian. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kannenberg, Elmar. University of Georgia; Estados Unidos
Fil: Allan Downie, J.. John Innes Institute; Reino Unido
Fil: Zorreguietaa, Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
description The formation of biofilms is an important survival strategy allowing rhizobia to live on soil particles and plant roots. Within the microcolonies of the biofilm developed by Rhizobium leguminosarum, rhizobial cells interact tightly through lateral and polar connections, forming organized and compact cell aggregates. These microcolonies are embedded in a biofilm matrix, whose main component is the acidic exopolysaccharide (EPS). Our work shows that the O-chain core region of the R. leguminosarum lipopolysaccharide (LPS) (which stretches out of the cell surface) strongly influences bacterial adhesive properties and cell-cell cohesion. Mutants defective in the O chain or O-chain core moiety developed premature microcolonies in which lateral bacterial contacts were greatly reduced. Furthermore, cell-cell interactions within the microcolonies of the LPS mutants were mediated mostly through their poles, resulting in a biofilm with an altered three-dimensional structure and increased thickness. In addition, on the root epidermis and on root hairs, O-antigen core-defective strains showed altered biofilm patterns with the typical microcolony compaction impaired. Taken together, these results indicate that the surface-exposed moiety of the LPS is crucial for proper cell-to-cell interactions and for the formation of robust biofilms on different surfaces.
publishDate 2015
dc.date.none.fl_str_mv 2015-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/38568
Russo, Daniela Marta; Abdian, Patricia Lorena; Posadas, Diana Maria; Williams, Alan; Vozza, Nicolas Federico; et al.; Lipopolysaccharide O-chain core region required for cellular cohesion and compaction of In Vitro and root biofilms developed by Rhizobium leguminosarum; American Society for Microbiology; Applied And Environmental Microbiology; 81; 3; 2-2015; 1013-1023
0099-2240
CONICET Digital
CONICET
url http://hdl.handle.net/11336/38568
identifier_str_mv Russo, Daniela Marta; Abdian, Patricia Lorena; Posadas, Diana Maria; Williams, Alan; Vozza, Nicolas Federico; et al.; Lipopolysaccharide O-chain core region required for cellular cohesion and compaction of In Vitro and root biofilms developed by Rhizobium leguminosarum; American Society for Microbiology; Applied And Environmental Microbiology; 81; 3; 2-2015; 1013-1023
0099-2240
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1128/AEM.03175-14
info:eu-repo/semantics/altIdentifier/url/http://aem.asm.org/content/early/2014/11/17/AEM.03175-14
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4292486/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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