A biomimetic approach to study human erythrocytes adhesion to vascular endothelium induced by alpha-hemolysin
- Autores
- Saffioti, Nicolas Andres; Leal Denis, Maria Florencia; Alvarez, Cora Lilia; Herlax, Vanesa Silvana; Schwarzbaum, Pablo Julio; Pallarola, Diego Andres
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- The α-haemolysin (HlyA) is an exotoxin produced by several strains of uropathogenic E. coli, one of the most important etiological agents of urinary infections. HlyA irreversibly binds to human erythrocytes (RBCs), initiating a degenerative process called eryptosis, characterized by biochemical and morphological changes such as phosphatidylserine (PS) exposure to the external layer of the plasma membrane of RBCs, shrinkage, and swelling. HlyA-induced PS externalization can lead to adhesion of RBCs to vascular endothelial cells (VECs). We studied the capacity of HlyA-treated RBCs to adhere to: 1- activated endothelial HMEC-1 cells under different flow conditions (dynamic adhesion); 2- surfaces homogeneously covered with extracellular matrix components in static conditions. Results showed that HlyA induced adhesion of RBCs to VECs at low flow (0.2 dyn/cm2), although higher flows induced rapid detachment. On the other hand, HlyA treatment also induced static adhesion of RBCs to collagen or fibrinogen. Thus, HlyA-treated RBCs displayed high but weak adherence to VECs under the experimental conditions. Additionally, to study the molecular mechanism of the HlyA-induced adhesion of RBCs we designed a biomimetic device to emulate the conditions of the blood vessels. The device was built by coupling a microfluidic chip to a nanopatterned surface (NPS) coated with gold nanoparticles (AuNPs). Different adhesion molecules from the VECs could be anchored to the AuNPs to mimic exposure of adhesion molecules of an activated endothelium whereas, the architecture of the capillaries was emulated by a network of microfluidic channels built in polydimethylsiloxane (PDMS). The synthesis of the device was optimized by following the process with quartz microbalance and fluorescence microscopy. Future experiments using the device will allow investigating HlyA-induced adhesion of RBCs to specific adhesion molecules under flow conditions.
Fil: Saffioti, Nicolas Andres. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martin. Instituto de Nanosistemas; Argentina
Fil: Leal Denis, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Alvarez, Cora Lilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Herlax, Vanesa Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Schwarzbaum, Pablo Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Pallarola, Diego Andres. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martin. Instituto de Nanosistemas; Argentina
Primeras Jornadas Virtuales de la Sociedad Argentina de Biofísica
Ciudad Autónoma de Buenos Aires
Argentina
Sociedad Argentina de Biofísica - Materia
-
MICROFLUIDICS
NANOPATTERNED
RTX TOXINS
ERIPTOSIS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/159529
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A biomimetic approach to study human erythrocytes adhesion to vascular endothelium induced by alpha-hemolysinSaffioti, Nicolas AndresLeal Denis, Maria FlorenciaAlvarez, Cora LiliaHerlax, Vanesa SilvanaSchwarzbaum, Pablo JulioPallarola, Diego AndresMICROFLUIDICSNANOPATTERNEDRTX TOXINSERIPTOSIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The α-haemolysin (HlyA) is an exotoxin produced by several strains of uropathogenic E. coli, one of the most important etiological agents of urinary infections. HlyA irreversibly binds to human erythrocytes (RBCs), initiating a degenerative process called eryptosis, characterized by biochemical and morphological changes such as phosphatidylserine (PS) exposure to the external layer of the plasma membrane of RBCs, shrinkage, and swelling. HlyA-induced PS externalization can lead to adhesion of RBCs to vascular endothelial cells (VECs). We studied the capacity of HlyA-treated RBCs to adhere to: 1- activated endothelial HMEC-1 cells under different flow conditions (dynamic adhesion); 2- surfaces homogeneously covered with extracellular matrix components in static conditions. Results showed that HlyA induced adhesion of RBCs to VECs at low flow (0.2 dyn/cm2), although higher flows induced rapid detachment. On the other hand, HlyA treatment also induced static adhesion of RBCs to collagen or fibrinogen. Thus, HlyA-treated RBCs displayed high but weak adherence to VECs under the experimental conditions. Additionally, to study the molecular mechanism of the HlyA-induced adhesion of RBCs we designed a biomimetic device to emulate the conditions of the blood vessels. The device was built by coupling a microfluidic chip to a nanopatterned surface (NPS) coated with gold nanoparticles (AuNPs). Different adhesion molecules from the VECs could be anchored to the AuNPs to mimic exposure of adhesion molecules of an activated endothelium whereas, the architecture of the capillaries was emulated by a network of microfluidic channels built in polydimethylsiloxane (PDMS). The synthesis of the device was optimized by following the process with quartz microbalance and fluorescence microscopy. Future experiments using the device will allow investigating HlyA-induced adhesion of RBCs to specific adhesion molecules under flow conditions.Fil: Saffioti, Nicolas Andres. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martin. Instituto de Nanosistemas; ArgentinaFil: Leal Denis, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Alvarez, Cora Lilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Herlax, Vanesa Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Schwarzbaum, Pablo Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Pallarola, Diego Andres. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martin. Instituto de Nanosistemas; ArgentinaPrimeras Jornadas Virtuales de la Sociedad Argentina de BiofísicaCiudad Autónoma de Buenos AiresArgentinaSociedad Argentina de BiofísicaSociedad Argentina de BiofíscaDelfino, Jose Maria2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectJornadaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/159529A biomimetic approach to study human erythrocytes adhesion to vascular endothelium induced by alpha-hemolysin; Primeras Jornadas Virtuales de la Sociedad Argentina de Biofísica; Ciudad Autónoma de Buenos Aires; Argentina; 2020; 80-80978-987-27591-8-6CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://biofisica.org.ar/reuniones-cientificas/reunionsab-previas/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:49:45Zoai:ri.conicet.gov.ar:11336/159529instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:49:45.306CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
A biomimetic approach to study human erythrocytes adhesion to vascular endothelium induced by alpha-hemolysin |
title |
A biomimetic approach to study human erythrocytes adhesion to vascular endothelium induced by alpha-hemolysin |
spellingShingle |
A biomimetic approach to study human erythrocytes adhesion to vascular endothelium induced by alpha-hemolysin Saffioti, Nicolas Andres MICROFLUIDICS NANOPATTERNED RTX TOXINS ERIPTOSIS |
title_short |
A biomimetic approach to study human erythrocytes adhesion to vascular endothelium induced by alpha-hemolysin |
title_full |
A biomimetic approach to study human erythrocytes adhesion to vascular endothelium induced by alpha-hemolysin |
title_fullStr |
A biomimetic approach to study human erythrocytes adhesion to vascular endothelium induced by alpha-hemolysin |
title_full_unstemmed |
A biomimetic approach to study human erythrocytes adhesion to vascular endothelium induced by alpha-hemolysin |
title_sort |
A biomimetic approach to study human erythrocytes adhesion to vascular endothelium induced by alpha-hemolysin |
dc.creator.none.fl_str_mv |
Saffioti, Nicolas Andres Leal Denis, Maria Florencia Alvarez, Cora Lilia Herlax, Vanesa Silvana Schwarzbaum, Pablo Julio Pallarola, Diego Andres |
author |
Saffioti, Nicolas Andres |
author_facet |
Saffioti, Nicolas Andres Leal Denis, Maria Florencia Alvarez, Cora Lilia Herlax, Vanesa Silvana Schwarzbaum, Pablo Julio Pallarola, Diego Andres |
author_role |
author |
author2 |
Leal Denis, Maria Florencia Alvarez, Cora Lilia Herlax, Vanesa Silvana Schwarzbaum, Pablo Julio Pallarola, Diego Andres |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Delfino, Jose Maria |
dc.subject.none.fl_str_mv |
MICROFLUIDICS NANOPATTERNED RTX TOXINS ERIPTOSIS |
topic |
MICROFLUIDICS NANOPATTERNED RTX TOXINS ERIPTOSIS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The α-haemolysin (HlyA) is an exotoxin produced by several strains of uropathogenic E. coli, one of the most important etiological agents of urinary infections. HlyA irreversibly binds to human erythrocytes (RBCs), initiating a degenerative process called eryptosis, characterized by biochemical and morphological changes such as phosphatidylserine (PS) exposure to the external layer of the plasma membrane of RBCs, shrinkage, and swelling. HlyA-induced PS externalization can lead to adhesion of RBCs to vascular endothelial cells (VECs). We studied the capacity of HlyA-treated RBCs to adhere to: 1- activated endothelial HMEC-1 cells under different flow conditions (dynamic adhesion); 2- surfaces homogeneously covered with extracellular matrix components in static conditions. Results showed that HlyA induced adhesion of RBCs to VECs at low flow (0.2 dyn/cm2), although higher flows induced rapid detachment. On the other hand, HlyA treatment also induced static adhesion of RBCs to collagen or fibrinogen. Thus, HlyA-treated RBCs displayed high but weak adherence to VECs under the experimental conditions. Additionally, to study the molecular mechanism of the HlyA-induced adhesion of RBCs we designed a biomimetic device to emulate the conditions of the blood vessels. The device was built by coupling a microfluidic chip to a nanopatterned surface (NPS) coated with gold nanoparticles (AuNPs). Different adhesion molecules from the VECs could be anchored to the AuNPs to mimic exposure of adhesion molecules of an activated endothelium whereas, the architecture of the capillaries was emulated by a network of microfluidic channels built in polydimethylsiloxane (PDMS). The synthesis of the device was optimized by following the process with quartz microbalance and fluorescence microscopy. Future experiments using the device will allow investigating HlyA-induced adhesion of RBCs to specific adhesion molecules under flow conditions. Fil: Saffioti, Nicolas Andres. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martin. Instituto de Nanosistemas; Argentina Fil: Leal Denis, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina Fil: Alvarez, Cora Lilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina Fil: Herlax, Vanesa Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina Fil: Schwarzbaum, Pablo Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina Fil: Pallarola, Diego Andres. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martin. Instituto de Nanosistemas; Argentina Primeras Jornadas Virtuales de la Sociedad Argentina de Biofísica Ciudad Autónoma de Buenos Aires Argentina Sociedad Argentina de Biofísica |
description |
The α-haemolysin (HlyA) is an exotoxin produced by several strains of uropathogenic E. coli, one of the most important etiological agents of urinary infections. HlyA irreversibly binds to human erythrocytes (RBCs), initiating a degenerative process called eryptosis, characterized by biochemical and morphological changes such as phosphatidylserine (PS) exposure to the external layer of the plasma membrane of RBCs, shrinkage, and swelling. HlyA-induced PS externalization can lead to adhesion of RBCs to vascular endothelial cells (VECs). We studied the capacity of HlyA-treated RBCs to adhere to: 1- activated endothelial HMEC-1 cells under different flow conditions (dynamic adhesion); 2- surfaces homogeneously covered with extracellular matrix components in static conditions. Results showed that HlyA induced adhesion of RBCs to VECs at low flow (0.2 dyn/cm2), although higher flows induced rapid detachment. On the other hand, HlyA treatment also induced static adhesion of RBCs to collagen or fibrinogen. Thus, HlyA-treated RBCs displayed high but weak adherence to VECs under the experimental conditions. Additionally, to study the molecular mechanism of the HlyA-induced adhesion of RBCs we designed a biomimetic device to emulate the conditions of the blood vessels. The device was built by coupling a microfluidic chip to a nanopatterned surface (NPS) coated with gold nanoparticles (AuNPs). Different adhesion molecules from the VECs could be anchored to the AuNPs to mimic exposure of adhesion molecules of an activated endothelium whereas, the architecture of the capillaries was emulated by a network of microfluidic channels built in polydimethylsiloxane (PDMS). The synthesis of the device was optimized by following the process with quartz microbalance and fluorescence microscopy. Future experiments using the device will allow investigating HlyA-induced adhesion of RBCs to specific adhesion molecules under flow conditions. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Jornada Book http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/159529 A biomimetic approach to study human erythrocytes adhesion to vascular endothelium induced by alpha-hemolysin; Primeras Jornadas Virtuales de la Sociedad Argentina de Biofísica; Ciudad Autónoma de Buenos Aires; Argentina; 2020; 80-80 978-987-27591-8-6 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/159529 |
identifier_str_mv |
A biomimetic approach to study human erythrocytes adhesion to vascular endothelium induced by alpha-hemolysin; Primeras Jornadas Virtuales de la Sociedad Argentina de Biofísica; Ciudad Autónoma de Buenos Aires; Argentina; 2020; 80-80 978-987-27591-8-6 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://biofisica.org.ar/reuniones-cientificas/reunionsab-previas/ |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.coverage.none.fl_str_mv |
Nacional |
dc.publisher.none.fl_str_mv |
Sociedad Argentina de Biofísca |
publisher.none.fl_str_mv |
Sociedad Argentina de Biofísca |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |