Modulation of the inflammation-coagulation interaction during pneumococcal pneumonia by immunobiotic Lactobacillus rhamnosus CRL1505: role of Toll-like receptor 2

Autores
Zelaya, María Hortensia del Rosario; Villena, Julio Cesar; Gramajo Lopez, Andres Daniel; Alvarez, Gladis Susana; Agüero, Graciela
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study evaluated the effect of nasally administered Lactobacillus rhamnosus CRL1505 on the immuno-coagulative response during pneumococcal infection in immunocompetent mice. In addition, we aimed to gain insight in the knowledge of the mechanism involved in the immunomodulatory effect of the L. rhamnosus CRL1505 strain by evaluating the role of Toll-like receptor (TLR)-2. Results showed that nasally administered L. rhamnosus CRL1505 effectively regulate inflammation and hemostatic alterations during the pneumococcal infection. Immunobiotic treatment significantly reduced permeability of the bronchoalveolar?capillarity barrier, and general cytotoxicity, decreasing lung tissue damage. The CRL1505 strain improved the production of TNF-α, IFN-γ, and IL-10 after pneumococcal challenge. In addition, increased thrombomodulin (TM) and reduced tissue factor (TF) expressions were found in lungs of L. rhamnosus CRL1505-treated mice. Moreover, we demonstrated for the first time that TLR2 signaling pathway has a role in the induction of IFN-γ and IL-10 and the reduction of TF. Results also allow us to speculate that a pattern recognition receptor (PRR), other than TLR2, may mediate the immunobiotic activity of L. rhamnosus CRL1505 and could explain changes in TNF-α and TM.
Fil: Zelaya, María Hortensia del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Gramajo Lopez, Andres Daniel. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Bioquimica Clinica Aplicada. Catedra de Bioquimica Clinica I; Argentina
Fil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Bioquimica Clinica Aplicada. Catedra de Bioquimica Clinica I; Argentina
Fil: Agüero, Graciela. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Bioquimica Clinica Aplicada. Catedra de Bioquimica Clinica I; Argentina
Materia
Hemostasis-Inflammation
Lactobacillus Rhamnosus Crl1505
Lung Injury
Toll-Like Receptor 2
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2775
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/2775
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Modulation of the inflammation-coagulation interaction during pneumococcal pneumonia by immunobiotic Lactobacillus rhamnosus CRL1505: role of Toll-like receptor 2Zelaya, María Hortensia del RosarioVillena, Julio CesarGramajo Lopez, Andres DanielAlvarez, Gladis SusanaAgüero, GracielaHemostasis-InflammationLactobacillus Rhamnosus Crl1505Lung InjuryToll-Like Receptor 2https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3This study evaluated the effect of nasally administered Lactobacillus rhamnosus CRL1505 on the immuno-coagulative response during pneumococcal infection in immunocompetent mice. In addition, we aimed to gain insight in the knowledge of the mechanism involved in the immunomodulatory effect of the L. rhamnosus CRL1505 strain by evaluating the role of Toll-like receptor (TLR)-2. Results showed that nasally administered L. rhamnosus CRL1505 effectively regulate inflammation and hemostatic alterations during the pneumococcal infection. Immunobiotic treatment significantly reduced permeability of the bronchoalveolar?capillarity barrier, and general cytotoxicity, decreasing lung tissue damage. The CRL1505 strain improved the production of TNF-α, IFN-γ, and IL-10 after pneumococcal challenge. In addition, increased thrombomodulin (TM) and reduced tissue factor (TF) expressions were found in lungs of L. rhamnosus CRL1505-treated mice. Moreover, we demonstrated for the first time that TLR2 signaling pathway has a role in the induction of IFN-γ and IL-10 and the reduction of TF. Results also allow us to speculate that a pattern recognition receptor (PRR), other than TLR2, may mediate the immunobiotic activity of L. rhamnosus CRL1505 and could explain changes in TNF-α and TM.Fil: Zelaya, María Hortensia del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); ArgentinaFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); ArgentinaFil: Gramajo Lopez, Andres Daniel. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Bioquimica Clinica Aplicada. Catedra de Bioquimica Clinica I; ArgentinaFil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Bioquimica Clinica Aplicada. Catedra de Bioquimica Clinica I; ArgentinaFil: Agüero, Graciela. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Bioquimica Clinica Aplicada. Catedra de Bioquimica Clinica I; ArgentinaWiley Blackwell Publishing, Inc2014-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2775Zelaya, María Hortensia del Rosario; Villena, Julio Cesar; Gramajo Lopez, Andres Daniel; Alvarez, Gladis Susana; Agüero, Graciela; Modulation of the inflammation-coagulation interaction during pneumococcal pneumonia by immunobiotic Lactobacillus rhamnosus CRL1505: role of Toll-like receptor 2; Wiley Blackwell Publishing, Inc; Microbiology And Immunology; 58; 7; 7-2014; 416-4260385-5600enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1348-0421info:eu-repo/semantics/altIdentifier/doi/10.1111/1348-0421.12163info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:19Zoai:ri.conicet.gov.ar:11336/2775instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:19.529CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Modulation of the inflammation-coagulation interaction during pneumococcal pneumonia by immunobiotic Lactobacillus rhamnosus CRL1505: role of Toll-like receptor 2
title Modulation of the inflammation-coagulation interaction during pneumococcal pneumonia by immunobiotic Lactobacillus rhamnosus CRL1505: role of Toll-like receptor 2
spellingShingle Modulation of the inflammation-coagulation interaction during pneumococcal pneumonia by immunobiotic Lactobacillus rhamnosus CRL1505: role of Toll-like receptor 2
Zelaya, María Hortensia del Rosario
Hemostasis-Inflammation
Lactobacillus Rhamnosus Crl1505
Lung Injury
Toll-Like Receptor 2
title_short Modulation of the inflammation-coagulation interaction during pneumococcal pneumonia by immunobiotic Lactobacillus rhamnosus CRL1505: role of Toll-like receptor 2
title_full Modulation of the inflammation-coagulation interaction during pneumococcal pneumonia by immunobiotic Lactobacillus rhamnosus CRL1505: role of Toll-like receptor 2
title_fullStr Modulation of the inflammation-coagulation interaction during pneumococcal pneumonia by immunobiotic Lactobacillus rhamnosus CRL1505: role of Toll-like receptor 2
title_full_unstemmed Modulation of the inflammation-coagulation interaction during pneumococcal pneumonia by immunobiotic Lactobacillus rhamnosus CRL1505: role of Toll-like receptor 2
title_sort Modulation of the inflammation-coagulation interaction during pneumococcal pneumonia by immunobiotic Lactobacillus rhamnosus CRL1505: role of Toll-like receptor 2
dc.creator.none.fl_str_mv Zelaya, María Hortensia del Rosario
Villena, Julio Cesar
Gramajo Lopez, Andres Daniel
Alvarez, Gladis Susana
Agüero, Graciela
author Zelaya, María Hortensia del Rosario
author_facet Zelaya, María Hortensia del Rosario
Villena, Julio Cesar
Gramajo Lopez, Andres Daniel
Alvarez, Gladis Susana
Agüero, Graciela
author_role author
author2 Villena, Julio Cesar
Gramajo Lopez, Andres Daniel
Alvarez, Gladis Susana
Agüero, Graciela
author2_role author
author
author
author
dc.subject.none.fl_str_mv Hemostasis-Inflammation
Lactobacillus Rhamnosus Crl1505
Lung Injury
Toll-Like Receptor 2
topic Hemostasis-Inflammation
Lactobacillus Rhamnosus Crl1505
Lung Injury
Toll-Like Receptor 2
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv This study evaluated the effect of nasally administered Lactobacillus rhamnosus CRL1505 on the immuno-coagulative response during pneumococcal infection in immunocompetent mice. In addition, we aimed to gain insight in the knowledge of the mechanism involved in the immunomodulatory effect of the L. rhamnosus CRL1505 strain by evaluating the role of Toll-like receptor (TLR)-2. Results showed that nasally administered L. rhamnosus CRL1505 effectively regulate inflammation and hemostatic alterations during the pneumococcal infection. Immunobiotic treatment significantly reduced permeability of the bronchoalveolar?capillarity barrier, and general cytotoxicity, decreasing lung tissue damage. The CRL1505 strain improved the production of TNF-α, IFN-γ, and IL-10 after pneumococcal challenge. In addition, increased thrombomodulin (TM) and reduced tissue factor (TF) expressions were found in lungs of L. rhamnosus CRL1505-treated mice. Moreover, we demonstrated for the first time that TLR2 signaling pathway has a role in the induction of IFN-γ and IL-10 and the reduction of TF. Results also allow us to speculate that a pattern recognition receptor (PRR), other than TLR2, may mediate the immunobiotic activity of L. rhamnosus CRL1505 and could explain changes in TNF-α and TM.
Fil: Zelaya, María Hortensia del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Gramajo Lopez, Andres Daniel. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Bioquimica Clinica Aplicada. Catedra de Bioquimica Clinica I; Argentina
Fil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Bioquimica Clinica Aplicada. Catedra de Bioquimica Clinica I; Argentina
Fil: Agüero, Graciela. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Bioquimica Clinica Aplicada. Catedra de Bioquimica Clinica I; Argentina
description This study evaluated the effect of nasally administered Lactobacillus rhamnosus CRL1505 on the immuno-coagulative response during pneumococcal infection in immunocompetent mice. In addition, we aimed to gain insight in the knowledge of the mechanism involved in the immunomodulatory effect of the L. rhamnosus CRL1505 strain by evaluating the role of Toll-like receptor (TLR)-2. Results showed that nasally administered L. rhamnosus CRL1505 effectively regulate inflammation and hemostatic alterations during the pneumococcal infection. Immunobiotic treatment significantly reduced permeability of the bronchoalveolar?capillarity barrier, and general cytotoxicity, decreasing lung tissue damage. The CRL1505 strain improved the production of TNF-α, IFN-γ, and IL-10 after pneumococcal challenge. In addition, increased thrombomodulin (TM) and reduced tissue factor (TF) expressions were found in lungs of L. rhamnosus CRL1505-treated mice. Moreover, we demonstrated for the first time that TLR2 signaling pathway has a role in the induction of IFN-γ and IL-10 and the reduction of TF. Results also allow us to speculate that a pattern recognition receptor (PRR), other than TLR2, may mediate the immunobiotic activity of L. rhamnosus CRL1505 and could explain changes in TNF-α and TM.
publishDate 2014
dc.date.none.fl_str_mv 2014-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2775
Zelaya, María Hortensia del Rosario; Villena, Julio Cesar; Gramajo Lopez, Andres Daniel; Alvarez, Gladis Susana; Agüero, Graciela; Modulation of the inflammation-coagulation interaction during pneumococcal pneumonia by immunobiotic Lactobacillus rhamnosus CRL1505: role of Toll-like receptor 2; Wiley Blackwell Publishing, Inc; Microbiology And Immunology; 58; 7; 7-2014; 416-426
0385-5600
url http://hdl.handle.net/11336/2775
identifier_str_mv Zelaya, María Hortensia del Rosario; Villena, Julio Cesar; Gramajo Lopez, Andres Daniel; Alvarez, Gladis Susana; Agüero, Graciela; Modulation of the inflammation-coagulation interaction during pneumococcal pneumonia by immunobiotic Lactobacillus rhamnosus CRL1505: role of Toll-like receptor 2; Wiley Blackwell Publishing, Inc; Microbiology And Immunology; 58; 7; 7-2014; 416-426
0385-5600
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1348-0421
info:eu-repo/semantics/altIdentifier/doi/10.1111/1348-0421.12163
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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