Role of VAMP7-Dependent Secretion of Reticulon 3 in Neurite Growth

Autores
Wojnacki, José; Nola, Sébastien; Bun, Philippe; Cholley, Béatrice; Filippini, Francesca; Pressé, Mary T.; Lipecka, Joanna; Man Lam, Sin; N'guyen, Julie; Simon, Axelle; Ouslimani, Amine; Shui, Guanghou; Fader Kaiser, Claudio Marcelo; Colombo, Maria Isabel; Guerrera, Ida Chiara; Galli, Thierry
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
VAMP7 is involved in autophagy and in exocytosis-mediated neurite growth, two yet unconnected cellular pathways. Here, we find that nutrient restriction and activation of autophagy stimulate axonal growth, while autophagy inhibition leads to loss of neuronal polarity. VAMP7 knockout (KO) neuronal cells show impaired neurite growth, whereas this process is increased in autophagy-null ATG5 KO cells. We find that endoplasmic reticulum (ER)-phagy-related LC3-interacting-region-containing proteins Atlastin 3 and Reticulon 3 (RTN3) are more abundant in autophagy-related protein ATG5 KO and less abundant in VAMP7 KO secretomes. Treatment of neuronal cells with ATG5 or VAMP7 KO conditioned medium does not recapitulate the effect of these KOs on neurite growth. A nanobody directed against VAMP7 inhibits axonal overgrowth induced by nutrient restriction. Furthermore, expression of the inhibitory Longin domain of VAMP7 impairs the subcellular localization of RTN3 in neurons. We propose that VAMP7-dependent secretion of RTN3 regulates neurite growth.
Fil: Wojnacki, José. Universite de Paris; Francia
Fil: Nola, Sébastien. Universite de Paris; Francia
Fil: Bun, Philippe. Universite de Paris; Francia
Fil: Cholley, Béatrice. Universite de Paris; Francia
Fil: Filippini, Francesca. Universite de Paris; Francia
Fil: Pressé, Mary T.. Universite de Paris; Francia
Fil: Lipecka, Joanna. Universite de Paris; Francia
Fil: Man Lam, Sin. Chinese Academy of Sciences; República de China
Fil: N'guyen, Julie. Universite de Paris; Francia
Fil: Simon, Axelle. Universite de Paris; Francia
Fil: Ouslimani, Amine. Universite de Paris; Francia
Fil: Shui, Guanghou. Chinese Academy of Sciences; República de China
Fil: Fader Kaiser, Claudio Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Colombo, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Guerrera, Ida Chiara. Universite de Paris; Francia
Fil: Galli, Thierry. Universite de Paris; Francia
Materia
AUTOPHAGY
ER-PHAGY
SECRETION
NEURON
DEVELOPMENT
SNARE
VAMP7
ATG5
RTN3
ATL3
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/141348
Acceder
id CONICETDig_0832d774a5311da6b4ef617eea24b4b8
oai_identifier_str oai:ri.conicet.gov.ar:11336/141348
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Role of VAMP7-Dependent Secretion of Reticulon 3 in Neurite GrowthWojnacki, JoséNola, SébastienBun, PhilippeCholley, BéatriceFilippini, FrancescaPressé, Mary T.Lipecka, JoannaMan Lam, SinN'guyen, JulieSimon, AxelleOuslimani, AmineShui, GuanghouFader Kaiser, Claudio MarceloColombo, Maria IsabelGuerrera, Ida ChiaraGalli, ThierryAUTOPHAGYER-PHAGYSECRETIONNEURONDEVELOPMENTSNAREVAMP7ATG5RTN3ATL3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1VAMP7 is involved in autophagy and in exocytosis-mediated neurite growth, two yet unconnected cellular pathways. Here, we find that nutrient restriction and activation of autophagy stimulate axonal growth, while autophagy inhibition leads to loss of neuronal polarity. VAMP7 knockout (KO) neuronal cells show impaired neurite growth, whereas this process is increased in autophagy-null ATG5 KO cells. We find that endoplasmic reticulum (ER)-phagy-related LC3-interacting-region-containing proteins Atlastin 3 and Reticulon 3 (RTN3) are more abundant in autophagy-related protein ATG5 KO and less abundant in VAMP7 KO secretomes. Treatment of neuronal cells with ATG5 or VAMP7 KO conditioned medium does not recapitulate the effect of these KOs on neurite growth. A nanobody directed against VAMP7 inhibits axonal overgrowth induced by nutrient restriction. Furthermore, expression of the inhibitory Longin domain of VAMP7 impairs the subcellular localization of RTN3 in neurons. We propose that VAMP7-dependent secretion of RTN3 regulates neurite growth.Fil: Wojnacki, José. Universite de Paris; FranciaFil: Nola, Sébastien. Universite de Paris; FranciaFil: Bun, Philippe. Universite de Paris; FranciaFil: Cholley, Béatrice. Universite de Paris; FranciaFil: Filippini, Francesca. Universite de Paris; FranciaFil: Pressé, Mary T.. Universite de Paris; FranciaFil: Lipecka, Joanna. Universite de Paris; FranciaFil: Man Lam, Sin. Chinese Academy of Sciences; República de ChinaFil: N'guyen, Julie. Universite de Paris; FranciaFil: Simon, Axelle. Universite de Paris; FranciaFil: Ouslimani, Amine. Universite de Paris; FranciaFil: Shui, Guanghou. Chinese Academy of Sciences; República de ChinaFil: Fader Kaiser, Claudio Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Colombo, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Guerrera, Ida Chiara. Universite de Paris; FranciaFil: Galli, Thierry. Universite de Paris; FranciaElsevier2020-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/141348Wojnacki, José; Nola, Sébastien; Bun, Philippe; Cholley, Béatrice; Filippini, Francesca; et al.; Role of VAMP7-Dependent Secretion of Reticulon 3 in Neurite Growth; Elsevier; Cell Reports; 33; 12; 12-2020; 1-242211-1247CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.celrep.2020.108536info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:40:06Zoai:ri.conicet.gov.ar:11336/141348instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:40:07.109CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role of VAMP7-Dependent Secretion of Reticulon 3 in Neurite Growth
title Role of VAMP7-Dependent Secretion of Reticulon 3 in Neurite Growth
spellingShingle Role of VAMP7-Dependent Secretion of Reticulon 3 in Neurite Growth
Wojnacki, José
AUTOPHAGY
ER-PHAGY
SECRETION
NEURON
DEVELOPMENT
SNARE
VAMP7
ATG5
RTN3
ATL3
title_short Role of VAMP7-Dependent Secretion of Reticulon 3 in Neurite Growth
title_full Role of VAMP7-Dependent Secretion of Reticulon 3 in Neurite Growth
title_fullStr Role of VAMP7-Dependent Secretion of Reticulon 3 in Neurite Growth
title_full_unstemmed Role of VAMP7-Dependent Secretion of Reticulon 3 in Neurite Growth
title_sort Role of VAMP7-Dependent Secretion of Reticulon 3 in Neurite Growth
dc.creator.none.fl_str_mv Wojnacki, José
Nola, Sébastien
Bun, Philippe
Cholley, Béatrice
Filippini, Francesca
Pressé, Mary T.
Lipecka, Joanna
Man Lam, Sin
N'guyen, Julie
Simon, Axelle
Ouslimani, Amine
Shui, Guanghou
Fader Kaiser, Claudio Marcelo
Colombo, Maria Isabel
Guerrera, Ida Chiara
Galli, Thierry
author Wojnacki, José
author_facet Wojnacki, José
Nola, Sébastien
Bun, Philippe
Cholley, Béatrice
Filippini, Francesca
Pressé, Mary T.
Lipecka, Joanna
Man Lam, Sin
N'guyen, Julie
Simon, Axelle
Ouslimani, Amine
Shui, Guanghou
Fader Kaiser, Claudio Marcelo
Colombo, Maria Isabel
Guerrera, Ida Chiara
Galli, Thierry
author_role author
author2 Nola, Sébastien
Bun, Philippe
Cholley, Béatrice
Filippini, Francesca
Pressé, Mary T.
Lipecka, Joanna
Man Lam, Sin
N'guyen, Julie
Simon, Axelle
Ouslimani, Amine
Shui, Guanghou
Fader Kaiser, Claudio Marcelo
Colombo, Maria Isabel
Guerrera, Ida Chiara
Galli, Thierry
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AUTOPHAGY
ER-PHAGY
SECRETION
NEURON
DEVELOPMENT
SNARE
VAMP7
ATG5
RTN3
ATL3
topic AUTOPHAGY
ER-PHAGY
SECRETION
NEURON
DEVELOPMENT
SNARE
VAMP7
ATG5
RTN3
ATL3
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv VAMP7 is involved in autophagy and in exocytosis-mediated neurite growth, two yet unconnected cellular pathways. Here, we find that nutrient restriction and activation of autophagy stimulate axonal growth, while autophagy inhibition leads to loss of neuronal polarity. VAMP7 knockout (KO) neuronal cells show impaired neurite growth, whereas this process is increased in autophagy-null ATG5 KO cells. We find that endoplasmic reticulum (ER)-phagy-related LC3-interacting-region-containing proteins Atlastin 3 and Reticulon 3 (RTN3) are more abundant in autophagy-related protein ATG5 KO and less abundant in VAMP7 KO secretomes. Treatment of neuronal cells with ATG5 or VAMP7 KO conditioned medium does not recapitulate the effect of these KOs on neurite growth. A nanobody directed against VAMP7 inhibits axonal overgrowth induced by nutrient restriction. Furthermore, expression of the inhibitory Longin domain of VAMP7 impairs the subcellular localization of RTN3 in neurons. We propose that VAMP7-dependent secretion of RTN3 regulates neurite growth.
Fil: Wojnacki, José. Universite de Paris; Francia
Fil: Nola, Sébastien. Universite de Paris; Francia
Fil: Bun, Philippe. Universite de Paris; Francia
Fil: Cholley, Béatrice. Universite de Paris; Francia
Fil: Filippini, Francesca. Universite de Paris; Francia
Fil: Pressé, Mary T.. Universite de Paris; Francia
Fil: Lipecka, Joanna. Universite de Paris; Francia
Fil: Man Lam, Sin. Chinese Academy of Sciences; República de China
Fil: N'guyen, Julie. Universite de Paris; Francia
Fil: Simon, Axelle. Universite de Paris; Francia
Fil: Ouslimani, Amine. Universite de Paris; Francia
Fil: Shui, Guanghou. Chinese Academy of Sciences; República de China
Fil: Fader Kaiser, Claudio Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Colombo, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Guerrera, Ida Chiara. Universite de Paris; Francia
Fil: Galli, Thierry. Universite de Paris; Francia
description VAMP7 is involved in autophagy and in exocytosis-mediated neurite growth, two yet unconnected cellular pathways. Here, we find that nutrient restriction and activation of autophagy stimulate axonal growth, while autophagy inhibition leads to loss of neuronal polarity. VAMP7 knockout (KO) neuronal cells show impaired neurite growth, whereas this process is increased in autophagy-null ATG5 KO cells. We find that endoplasmic reticulum (ER)-phagy-related LC3-interacting-region-containing proteins Atlastin 3 and Reticulon 3 (RTN3) are more abundant in autophagy-related protein ATG5 KO and less abundant in VAMP7 KO secretomes. Treatment of neuronal cells with ATG5 or VAMP7 KO conditioned medium does not recapitulate the effect of these KOs on neurite growth. A nanobody directed against VAMP7 inhibits axonal overgrowth induced by nutrient restriction. Furthermore, expression of the inhibitory Longin domain of VAMP7 impairs the subcellular localization of RTN3 in neurons. We propose that VAMP7-dependent secretion of RTN3 regulates neurite growth.
publishDate 2020
dc.date.none.fl_str_mv 2020-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/141348
Wojnacki, José; Nola, Sébastien; Bun, Philippe; Cholley, Béatrice; Filippini, Francesca; et al.; Role of VAMP7-Dependent Secretion of Reticulon 3 in Neurite Growth; Elsevier; Cell Reports; 33; 12; 12-2020; 1-24
2211-1247
CONICET Digital
CONICET
url http://hdl.handle.net/11336/141348
identifier_str_mv Wojnacki, José; Nola, Sébastien; Bun, Philippe; Cholley, Béatrice; Filippini, Francesca; et al.; Role of VAMP7-Dependent Secretion of Reticulon 3 in Neurite Growth; Elsevier; Cell Reports; 33; 12; 12-2020; 1-24
2211-1247
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.celrep.2020.108536
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613268846411776
score 13.070432

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