Fumonisins in maize and gluten meal analysed in Argentinean wet milling industrial plants by ELISA compared with HPLC-FLD method

Autores
Coronel, María Bernarda; Vicente, Sebastián; Resnik, Silvia Liliana; Alzamora, Stella Maris; Pacin, Ana
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fumonisins are frequent contaminants of maize. Wet milling industrial plants in Argentina control not only maize, but the fractions of the process. The performance of two ELISA test kits (G and K) from different brands used in six plants to determine fumonisins in maize and gluten meal was evaluated by comparison with HPLC results. ELISA determinations in maize (n = 43) and gluten meal (n = 45) were carried out in the plants and HPLC analysis was done in our laboratory. No significant differences (P > 0.05) were found in most cases when fumonisins concentrations were within the ELISA quantification range. Thus, ELISA tests performed according to validated protocols are useful tools for screening purposes, but levels nearest the settled limits for rejection of lots should be confirmed by HPLC because of the high relative standard deviation of ELISA analyses. The contamination pattern of fumonisins in gluten meal was different from that of maize, with higher levels of FB2 than those of FB1.
Fil: Coronel, María Bernarda. Fundación de Investigaciones Científicas "Teresa Benedicta de la Cruz"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vicente, Sebastián. Fundación de Investigaciones Científicas "Teresa Benedicta de la Cruz"; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Resnik, Silvia Liliana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alzamora, Stella Maris. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pacin, Ana. Fundación de Investigaciones Científicas "Teresa Benedicta de la Cruz"; Argentina
Materia
Elisa
Fumonisins
Gluten Meal
Hplc
Maize
Wet Milling
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/59635
Acceder
id CONICETDig_07ccd94340d6149ce85143cf73187384
oai_identifier_str oai:ri.conicet.gov.ar:11336/59635
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Fumonisins in maize and gluten meal analysed in Argentinean wet milling industrial plants by ELISA compared with HPLC-FLD methodCoronel, María BernardaVicente, SebastiánResnik, Silvia LilianaAlzamora, Stella MarisPacin, AnaElisaFumonisinsGluten MealHplcMaizeWet Millinghttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Fumonisins are frequent contaminants of maize. Wet milling industrial plants in Argentina control not only maize, but the fractions of the process. The performance of two ELISA test kits (G and K) from different brands used in six plants to determine fumonisins in maize and gluten meal was evaluated by comparison with HPLC results. ELISA determinations in maize (n = 43) and gluten meal (n = 45) were carried out in the plants and HPLC analysis was done in our laboratory. No significant differences (P > 0.05) were found in most cases when fumonisins concentrations were within the ELISA quantification range. Thus, ELISA tests performed according to validated protocols are useful tools for screening purposes, but levels nearest the settled limits for rejection of lots should be confirmed by HPLC because of the high relative standard deviation of ELISA analyses. The contamination pattern of fumonisins in gluten meal was different from that of maize, with higher levels of FB2 than those of FB1.Fil: Coronel, María Bernarda. Fundación de Investigaciones Científicas "Teresa Benedicta de la Cruz"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vicente, Sebastián. Fundación de Investigaciones Científicas "Teresa Benedicta de la Cruz"; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Resnik, Silvia Liliana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alzamora, Stella Maris. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pacin, Ana. Fundación de Investigaciones Científicas "Teresa Benedicta de la Cruz"; ArgentinaElsevier2016-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/59635Coronel, María Bernarda; Vicente, Sebastián; Resnik, Silvia Liliana; Alzamora, Stella Maris; Pacin, Ana; Fumonisins in maize and gluten meal analysed in Argentinean wet milling industrial plants by ELISA compared with HPLC-FLD method; Elsevier; Food Control; 67; 9-2016; 285-2910956-7135CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodcont.2016.02.052info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0956713516300986info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:59Zoai:ri.conicet.gov.ar:11336/59635instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:59.349CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fumonisins in maize and gluten meal analysed in Argentinean wet milling industrial plants by ELISA compared with HPLC-FLD method
title Fumonisins in maize and gluten meal analysed in Argentinean wet milling industrial plants by ELISA compared with HPLC-FLD method
spellingShingle Fumonisins in maize and gluten meal analysed in Argentinean wet milling industrial plants by ELISA compared with HPLC-FLD method
Coronel, María Bernarda
Elisa
Fumonisins
Gluten Meal
Hplc
Maize
Wet Milling
title_short Fumonisins in maize and gluten meal analysed in Argentinean wet milling industrial plants by ELISA compared with HPLC-FLD method
title_full Fumonisins in maize and gluten meal analysed in Argentinean wet milling industrial plants by ELISA compared with HPLC-FLD method
title_fullStr Fumonisins in maize and gluten meal analysed in Argentinean wet milling industrial plants by ELISA compared with HPLC-FLD method
title_full_unstemmed Fumonisins in maize and gluten meal analysed in Argentinean wet milling industrial plants by ELISA compared with HPLC-FLD method
title_sort Fumonisins in maize and gluten meal analysed in Argentinean wet milling industrial plants by ELISA compared with HPLC-FLD method
dc.creator.none.fl_str_mv Coronel, María Bernarda
Vicente, Sebastián
Resnik, Silvia Liliana
Alzamora, Stella Maris
Pacin, Ana
author Coronel, María Bernarda
author_facet Coronel, María Bernarda
Vicente, Sebastián
Resnik, Silvia Liliana
Alzamora, Stella Maris
Pacin, Ana
author_role author
author2 Vicente, Sebastián
Resnik, Silvia Liliana
Alzamora, Stella Maris
Pacin, Ana
author2_role author
author
author
author
dc.subject.none.fl_str_mv Elisa
Fumonisins
Gluten Meal
Hplc
Maize
Wet Milling
topic Elisa
Fumonisins
Gluten Meal
Hplc
Maize
Wet Milling
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Fumonisins are frequent contaminants of maize. Wet milling industrial plants in Argentina control not only maize, but the fractions of the process. The performance of two ELISA test kits (G and K) from different brands used in six plants to determine fumonisins in maize and gluten meal was evaluated by comparison with HPLC results. ELISA determinations in maize (n = 43) and gluten meal (n = 45) were carried out in the plants and HPLC analysis was done in our laboratory. No significant differences (P > 0.05) were found in most cases when fumonisins concentrations were within the ELISA quantification range. Thus, ELISA tests performed according to validated protocols are useful tools for screening purposes, but levels nearest the settled limits for rejection of lots should be confirmed by HPLC because of the high relative standard deviation of ELISA analyses. The contamination pattern of fumonisins in gluten meal was different from that of maize, with higher levels of FB2 than those of FB1.
Fil: Coronel, María Bernarda. Fundación de Investigaciones Científicas "Teresa Benedicta de la Cruz"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vicente, Sebastián. Fundación de Investigaciones Científicas "Teresa Benedicta de la Cruz"; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Resnik, Silvia Liliana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alzamora, Stella Maris. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pacin, Ana. Fundación de Investigaciones Científicas "Teresa Benedicta de la Cruz"; Argentina
description Fumonisins are frequent contaminants of maize. Wet milling industrial plants in Argentina control not only maize, but the fractions of the process. The performance of two ELISA test kits (G and K) from different brands used in six plants to determine fumonisins in maize and gluten meal was evaluated by comparison with HPLC results. ELISA determinations in maize (n = 43) and gluten meal (n = 45) were carried out in the plants and HPLC analysis was done in our laboratory. No significant differences (P > 0.05) were found in most cases when fumonisins concentrations were within the ELISA quantification range. Thus, ELISA tests performed according to validated protocols are useful tools for screening purposes, but levels nearest the settled limits for rejection of lots should be confirmed by HPLC because of the high relative standard deviation of ELISA analyses. The contamination pattern of fumonisins in gluten meal was different from that of maize, with higher levels of FB2 than those of FB1.
publishDate 2016
dc.date.none.fl_str_mv 2016-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/59635
Coronel, María Bernarda; Vicente, Sebastián; Resnik, Silvia Liliana; Alzamora, Stella Maris; Pacin, Ana; Fumonisins in maize and gluten meal analysed in Argentinean wet milling industrial plants by ELISA compared with HPLC-FLD method; Elsevier; Food Control; 67; 9-2016; 285-291
0956-7135
CONICET Digital
CONICET
url http://hdl.handle.net/11336/59635
identifier_str_mv Coronel, María Bernarda; Vicente, Sebastián; Resnik, Silvia Liliana; Alzamora, Stella Maris; Pacin, Ana; Fumonisins in maize and gluten meal analysed in Argentinean wet milling industrial plants by ELISA compared with HPLC-FLD method; Elsevier; Food Control; 67; 9-2016; 285-291
0956-7135
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodcont.2016.02.052
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0956713516300986
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613086140432384
score 13.070432

Publicaciones similares