Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice

Autores
Refojo, Damian; Kovalovsky, Damian; Young, Juan I.; Rubinstein, Marcelo; Holsboer, Florian; Reul, Johannes M. H. M.; Low, Malcolm J.; Arzt, Eduardo Simon
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We used β-endorphin-deficient mice as a novel approach to confirm the physiological role that opioid peptides play in the development or regulation of the immune system. We found that mice lacking β-endorphin possessed an enhanced immune response, measured in terms of splenocyte proliferation and interleukin (IL)-2 mRNA levels, in vitro production of the splenic macrophage inflammatory cytokines IL-6 and Tumor Necrosis Factor (TNF)-α and plasma IL-6 following lipopolysaccharide (LPS) administration. β-Endorphin-deficient mice had attenuated increases of plasma ACTH and corticosterone levels in response to LPS. These results are consistent with a postulated inhibitory role of endogenous β-endorphin on the immune system at multiple levels.
Fil: Refojo, Damian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Fisiología y Biología Molecular; Argentina
Fil: Kovalovsky, Damian. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Fisiología y Biología Molecular; Argentina
Fil: Young, Juan I.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Rubinstein, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Holsboer, Florian. Max Planck Institute of Psychiatry; Alemania
Fil: Reul, Johannes M. H. M.. Max Planck Institute of Psychiatry; Alemania
Fil: Low, Malcolm J.. Oregon Health and Science University; Estados Unidos
Fil: Arzt, Eduardo Simon. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Fisiología y Biología Molecular; Argentina
Materia
Β-Endorphin
Cytokines
Hpa Axis
Neuroimmunology
Splenocyte
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79750
Acceder
id CONICETDig_07a36521ff1bd3eded6e9c9e2d99fc97
oai_identifier_str oai:ri.conicet.gov.ar:11336/79750
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient miceRefojo, DamianKovalovsky, DamianYoung, Juan I.Rubinstein, MarceloHolsboer, FlorianReul, Johannes M. H. M.Low, Malcolm J.Arzt, Eduardo SimonΒ-EndorphinCytokinesHpa AxisNeuroimmunologySplenocytehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3We used β-endorphin-deficient mice as a novel approach to confirm the physiological role that opioid peptides play in the development or regulation of the immune system. We found that mice lacking β-endorphin possessed an enhanced immune response, measured in terms of splenocyte proliferation and interleukin (IL)-2 mRNA levels, in vitro production of the splenic macrophage inflammatory cytokines IL-6 and Tumor Necrosis Factor (TNF)-α and plasma IL-6 following lipopolysaccharide (LPS) administration. β-Endorphin-deficient mice had attenuated increases of plasma ACTH and corticosterone levels in response to LPS. These results are consistent with a postulated inhibitory role of endogenous β-endorphin on the immune system at multiple levels.Fil: Refojo, Damian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Fisiología y Biología Molecular; ArgentinaFil: Kovalovsky, Damian. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Fisiología y Biología Molecular; ArgentinaFil: Young, Juan I.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Rubinstein, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Holsboer, Florian. Max Planck Institute of Psychiatry; AlemaniaFil: Reul, Johannes M. H. M.. Max Planck Institute of Psychiatry; AlemaniaFil: Low, Malcolm J.. Oregon Health and Science University; Estados UnidosFil: Arzt, Eduardo Simon. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Fisiología y Biología Molecular; ArgentinaElsevier Science2002-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79750Refojo, Damian; Kovalovsky, Damian; Young, Juan I.; Rubinstein, Marcelo; Holsboer, Florian; et al.; Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice; Elsevier Science; Journal of Neuroimmunology; 131; 1-2; 10-2002; 126-1340165-5728CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/12458044info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0165572802002680info:eu-repo/semantics/altIdentifier/doi/10.1016/S0165-5728(02)00268-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T12:07:22Zoai:ri.conicet.gov.ar:11336/79750instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 12:07:23.121CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice
title Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice
spellingShingle Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice
Refojo, Damian
Β-Endorphin
Cytokines
Hpa Axis
Neuroimmunology
Splenocyte
title_short Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice
title_full Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice
title_fullStr Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice
title_full_unstemmed Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice
title_sort Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice
dc.creator.none.fl_str_mv Refojo, Damian
Kovalovsky, Damian
Young, Juan I.
Rubinstein, Marcelo
Holsboer, Florian
Reul, Johannes M. H. M.
Low, Malcolm J.
Arzt, Eduardo Simon
author Refojo, Damian
author_facet Refojo, Damian
Kovalovsky, Damian
Young, Juan I.
Rubinstein, Marcelo
Holsboer, Florian
Reul, Johannes M. H. M.
Low, Malcolm J.
Arzt, Eduardo Simon
author_role author
author2 Kovalovsky, Damian
Young, Juan I.
Rubinstein, Marcelo
Holsboer, Florian
Reul, Johannes M. H. M.
Low, Malcolm J.
Arzt, Eduardo Simon
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Β-Endorphin
Cytokines
Hpa Axis
Neuroimmunology
Splenocyte
topic Β-Endorphin
Cytokines
Hpa Axis
Neuroimmunology
Splenocyte
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv We used β-endorphin-deficient mice as a novel approach to confirm the physiological role that opioid peptides play in the development or regulation of the immune system. We found that mice lacking β-endorphin possessed an enhanced immune response, measured in terms of splenocyte proliferation and interleukin (IL)-2 mRNA levels, in vitro production of the splenic macrophage inflammatory cytokines IL-6 and Tumor Necrosis Factor (TNF)-α and plasma IL-6 following lipopolysaccharide (LPS) administration. β-Endorphin-deficient mice had attenuated increases of plasma ACTH and corticosterone levels in response to LPS. These results are consistent with a postulated inhibitory role of endogenous β-endorphin on the immune system at multiple levels.
Fil: Refojo, Damian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Fisiología y Biología Molecular; Argentina
Fil: Kovalovsky, Damian. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Fisiología y Biología Molecular; Argentina
Fil: Young, Juan I.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Rubinstein, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Holsboer, Florian. Max Planck Institute of Psychiatry; Alemania
Fil: Reul, Johannes M. H. M.. Max Planck Institute of Psychiatry; Alemania
Fil: Low, Malcolm J.. Oregon Health and Science University; Estados Unidos
Fil: Arzt, Eduardo Simon. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Fisiología y Biología Molecular; Argentina
description We used β-endorphin-deficient mice as a novel approach to confirm the physiological role that opioid peptides play in the development or regulation of the immune system. We found that mice lacking β-endorphin possessed an enhanced immune response, measured in terms of splenocyte proliferation and interleukin (IL)-2 mRNA levels, in vitro production of the splenic macrophage inflammatory cytokines IL-6 and Tumor Necrosis Factor (TNF)-α and plasma IL-6 following lipopolysaccharide (LPS) administration. β-Endorphin-deficient mice had attenuated increases of plasma ACTH and corticosterone levels in response to LPS. These results are consistent with a postulated inhibitory role of endogenous β-endorphin on the immune system at multiple levels.
publishDate 2002
dc.date.none.fl_str_mv 2002-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79750
Refojo, Damian; Kovalovsky, Damian; Young, Juan I.; Rubinstein, Marcelo; Holsboer, Florian; et al.; Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice; Elsevier Science; Journal of Neuroimmunology; 131; 1-2; 10-2002; 126-134
0165-5728
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79750
identifier_str_mv Refojo, Damian; Kovalovsky, Damian; Young, Juan I.; Rubinstein, Marcelo; Holsboer, Florian; et al.; Increased splenocyte proliferative response and cytokine production in β-endorphin-deficient mice; Elsevier Science; Journal of Neuroimmunology; 131; 1-2; 10-2002; 126-134
0165-5728
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/12458044
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0165572802002680
info:eu-repo/semantics/altIdentifier/doi/10.1016/S0165-5728(02)00268-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1847426876002271232
score 13.10058

Publicaciones similares