Brucella abortus-infected platelets adctivate the endothelium and promote the migration of monocytes towards the site of infection

Autores
Trotta, Aldana; Milillo, María Ayelén; Rodríguez, Ana María; Balboa, Luciana; Delpino, María Victoria; Giambartolomei, Guillermo Hernan; Barrionuevo, Paula
Año de publicación
2018
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Brucellosis is an infectious disease elicited by bacteria of the genusBrucella. Platelets have recently got involved in the modulationof innate and adaptive immune responses. We have previously reportedthat platelets act as carriers of bacteria, promoting the invasionof monocytes. Thus, the aim of this study was to further investigatethe role of platelets in the immune response against Brucella.First, we wondered whether the presence of platelets modulates thetime course of B. abortus infection. For this, THP-1 cells (humanmonocytic cell line) were infected with B. abortus in presence orabsence of platelets. Then, extracellular bacteria were killed andcells were incubated for different times. Our results demonstratethat the presence of platelets significantly increased the percentageof B. abortus-infected THP-1 cells at early time-points (p<0.001).Nevertheless, the presence of platelets subsequently improvedthe contention of the infection. Taking into consideration that B.abortus localization within different tissues requires its extravasationacross the endothelium, our next aim was to study the role ofplatelets in the modulation of monocytes extravasation in the contextof B. abortus-mediated infection. We first studied the ability ofplatelets to recruit monocytes. Our results showed that supernatantscollected from infected platelets promote the transmigrationof monocytes (p<0.01). Moreover, the pre-treatment of monocyteswith this supernatant enhance the responsiveness of monocytestowards other chemoattractant stimuli (p<0.01). Finally, we studiedthe ability of platelets to activate the endothelium. For this, HMECcells (human endothelial cell line) were stimulated with supernatantscollected from B. abortus-infected platelets. This supernatant stimulatedthe expression of ICAM-1 (CD54) (p<0.01). At the same time,it enhanced the secretion of both IL-8 and MCP-1 (p<0.01). Theseresults showed that infected platelets are able to activate the endotheliumand promote the migration of monocytes towards the site ofthe infection.
Fil: Trotta, Aldana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Milillo, María Ayelén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Rodríguez, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Balboa, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Delpino, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Giambartolomei, Guillermo Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Barrionuevo, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
Sociedad Argentina de Virología
Asociación Argentina de Nanomedicinas
Materia
PLATELETS
BRUCELLA
MACROPHAGE
INFECTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/244658
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/244658
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Brucella abortus-infected platelets adctivate the endothelium and promote the migration of monocytes towards the site of infectionTrotta, AldanaMilillo, María AyelénRodríguez, Ana MaríaBalboa, LucianaDelpino, María VictoriaGiambartolomei, Guillermo HernanBarrionuevo, PaulaPLATELETSBRUCELLAMACROPHAGEINFECTIONhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Brucellosis is an infectious disease elicited by bacteria of the genusBrucella. Platelets have recently got involved in the modulationof innate and adaptive immune responses. We have previously reportedthat platelets act as carriers of bacteria, promoting the invasionof monocytes. Thus, the aim of this study was to further investigatethe role of platelets in the immune response against Brucella.First, we wondered whether the presence of platelets modulates thetime course of B. abortus infection. For this, THP-1 cells (humanmonocytic cell line) were infected with B. abortus in presence orabsence of platelets. Then, extracellular bacteria were killed andcells were incubated for different times. Our results demonstratethat the presence of platelets significantly increased the percentageof B. abortus-infected THP-1 cells at early time-points (p<0.001).Nevertheless, the presence of platelets subsequently improvedthe contention of the infection. Taking into consideration that B.abortus localization within different tissues requires its extravasationacross the endothelium, our next aim was to study the role ofplatelets in the modulation of monocytes extravasation in the contextof B. abortus-mediated infection. We first studied the ability ofplatelets to recruit monocytes. Our results showed that supernatantscollected from infected platelets promote the transmigrationof monocytes (p<0.01). Moreover, the pre-treatment of monocyteswith this supernatant enhance the responsiveness of monocytestowards other chemoattractant stimuli (p<0.01). Finally, we studiedthe ability of platelets to activate the endothelium. For this, HMECcells (human endothelial cell line) were stimulated with supernatantscollected from B. abortus-infected platelets. This supernatant stimulatedthe expression of ICAM-1 (CD54) (p<0.01). At the same time,it enhanced the secretion of both IL-8 and MCP-1 (p<0.01). Theseresults showed that infected platelets are able to activate the endotheliumand promote the migration of monocytes towards the site ofthe infection.Fil: Trotta, Aldana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Milillo, María Ayelén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Rodríguez, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Balboa, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Delpino, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Giambartolomei, Guillermo Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Barrionuevo, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaLXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de FisiologíaMar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaSociedad Argentina de FisiologíaSociedad Argentina de VirologíaAsociación Argentina de NanomedicinasFundación Revista Medicina2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/244658Brucella abortus-infected platelets adctivate the endothelium and promote the migration of monocytes towards the site of infection; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2018; 1-50025-7680CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2010-a-2019/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:14:40Zoai:ri.conicet.gov.ar:11336/244658instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:14:40.932CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Brucella abortus-infected platelets adctivate the endothelium and promote the migration of monocytes towards the site of infection
title Brucella abortus-infected platelets adctivate the endothelium and promote the migration of monocytes towards the site of infection
spellingShingle Brucella abortus-infected platelets adctivate the endothelium and promote the migration of monocytes towards the site of infection
Trotta, Aldana
PLATELETS
BRUCELLA
MACROPHAGE
INFECTION
title_short Brucella abortus-infected platelets adctivate the endothelium and promote the migration of monocytes towards the site of infection
title_full Brucella abortus-infected platelets adctivate the endothelium and promote the migration of monocytes towards the site of infection
title_fullStr Brucella abortus-infected platelets adctivate the endothelium and promote the migration of monocytes towards the site of infection
title_full_unstemmed Brucella abortus-infected platelets adctivate the endothelium and promote the migration of monocytes towards the site of infection
title_sort Brucella abortus-infected platelets adctivate the endothelium and promote the migration of monocytes towards the site of infection
dc.creator.none.fl_str_mv Trotta, Aldana
Milillo, María Ayelén
Rodríguez, Ana María
Balboa, Luciana
Delpino, María Victoria
Giambartolomei, Guillermo Hernan
Barrionuevo, Paula
author Trotta, Aldana
author_facet Trotta, Aldana
Milillo, María Ayelén
Rodríguez, Ana María
Balboa, Luciana
Delpino, María Victoria
Giambartolomei, Guillermo Hernan
Barrionuevo, Paula
author_role author
author2 Milillo, María Ayelén
Rodríguez, Ana María
Balboa, Luciana
Delpino, María Victoria
Giambartolomei, Guillermo Hernan
Barrionuevo, Paula
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv PLATELETS
BRUCELLA
MACROPHAGE
INFECTION
topic PLATELETS
BRUCELLA
MACROPHAGE
INFECTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Brucellosis is an infectious disease elicited by bacteria of the genusBrucella. Platelets have recently got involved in the modulationof innate and adaptive immune responses. We have previously reportedthat platelets act as carriers of bacteria, promoting the invasionof monocytes. Thus, the aim of this study was to further investigatethe role of platelets in the immune response against Brucella.First, we wondered whether the presence of platelets modulates thetime course of B. abortus infection. For this, THP-1 cells (humanmonocytic cell line) were infected with B. abortus in presence orabsence of platelets. Then, extracellular bacteria were killed andcells were incubated for different times. Our results demonstratethat the presence of platelets significantly increased the percentageof B. abortus-infected THP-1 cells at early time-points (p<0.001).Nevertheless, the presence of platelets subsequently improvedthe contention of the infection. Taking into consideration that B.abortus localization within different tissues requires its extravasationacross the endothelium, our next aim was to study the role ofplatelets in the modulation of monocytes extravasation in the contextof B. abortus-mediated infection. We first studied the ability ofplatelets to recruit monocytes. Our results showed that supernatantscollected from infected platelets promote the transmigrationof monocytes (p<0.01). Moreover, the pre-treatment of monocyteswith this supernatant enhance the responsiveness of monocytestowards other chemoattractant stimuli (p<0.01). Finally, we studiedthe ability of platelets to activate the endothelium. For this, HMECcells (human endothelial cell line) were stimulated with supernatantscollected from B. abortus-infected platelets. This supernatant stimulatedthe expression of ICAM-1 (CD54) (p<0.01). At the same time,it enhanced the secretion of both IL-8 and MCP-1 (p<0.01). Theseresults showed that infected platelets are able to activate the endotheliumand promote the migration of monocytes towards the site ofthe infection.
Fil: Trotta, Aldana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Milillo, María Ayelén. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Rodríguez, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Balboa, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Delpino, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Giambartolomei, Guillermo Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Barrionuevo, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
Sociedad Argentina de Virología
Asociación Argentina de Nanomedicinas
description Brucellosis is an infectious disease elicited by bacteria of the genusBrucella. Platelets have recently got involved in the modulationof innate and adaptive immune responses. We have previously reportedthat platelets act as carriers of bacteria, promoting the invasionof monocytes. Thus, the aim of this study was to further investigatethe role of platelets in the immune response against Brucella.First, we wondered whether the presence of platelets modulates thetime course of B. abortus infection. For this, THP-1 cells (humanmonocytic cell line) were infected with B. abortus in presence orabsence of platelets. Then, extracellular bacteria were killed andcells were incubated for different times. Our results demonstratethat the presence of platelets significantly increased the percentageof B. abortus-infected THP-1 cells at early time-points (p<0.001).Nevertheless, the presence of platelets subsequently improvedthe contention of the infection. Taking into consideration that B.abortus localization within different tissues requires its extravasationacross the endothelium, our next aim was to study the role ofplatelets in the modulation of monocytes extravasation in the contextof B. abortus-mediated infection. We first studied the ability ofplatelets to recruit monocytes. Our results showed that supernatantscollected from infected platelets promote the transmigrationof monocytes (p<0.01). Moreover, the pre-treatment of monocyteswith this supernatant enhance the responsiveness of monocytestowards other chemoattractant stimuli (p<0.01). Finally, we studiedthe ability of platelets to activate the endothelium. For this, HMECcells (human endothelial cell line) were stimulated with supernatantscollected from B. abortus-infected platelets. This supernatant stimulatedthe expression of ICAM-1 (CD54) (p<0.01). At the same time,it enhanced the secretion of both IL-8 and MCP-1 (p<0.01). Theseresults showed that infected platelets are able to activate the endotheliumand promote the migration of monocytes towards the site ofthe infection.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/244658
Brucella abortus-infected platelets adctivate the endothelium and promote the migration of monocytes towards the site of infection; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2018; 1-5
0025-7680
CONICET Digital
CONICET
url http://hdl.handle.net/11336/244658
identifier_str_mv Brucella abortus-infected platelets adctivate the endothelium and promote the migration of monocytes towards the site of infection; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVI Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2018; 1-5
0025-7680
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2010-a-2019/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/msword
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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