AT1 receptors in striatum DA-uptake: Crucial role and relevance in an Amphetamine-sensitization model of Schizophrenia

Autores
Basmadjian, Osvaldo Martin; Montemerlo, Antonella Evelin; Rivas, Gustavo Adolfo; Baiardi, Gustavo Carlos; Rubianes, María Dolores; Bregonzio, Claudia
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Limbic dopamine (DA) hyperactivity, a hallmark of amphetamine (AMPH) exposure, it is considered as a neurochemical feature involved in the expression of schizophrenic positive symptoms. DA neurotransmission dynamics is regulated by the uptake of extracellular transmitter at presynaptic neurons through specific transporter. Angiotensin II, through AT1 receptors (AT1-R), modulates DA neurotransmission at limbic areas. Herein, we studied AT1-R involvement after AMPH exposure on: a) development and expression of behavioral sensitization, b) in vitro striatum DA uptake. To these purposes male Wistar rats (250-300 g) were daily administered with d-AMPH (2.5 mg/kg i.p.) for 5 days. After 3 weeks of withdrawal, the behavioral sensitization was evaluated measuring locomotor activity. The AT1-R blocker, Candesartan (CV 3 mg/kg p.o.), was administered daily for 10 days, starting 5 days prior to the first AMPH injection in the prevention sensitization protocol. In the reversion protocol, either, CV or aripiprazole (antipsychotic drug widely use partial agonist of D2 receptors), were administered for 5 days starting 2 weeks after the last AMPH injection. DA uptake was measured in homogenized striatum using an electrochemical sensor, based on glassy carbon electrode modified with carbon nanotubes and polyethylenimine by amperometry. The results were analyzed by 2-way ANOVA followed by Bonferroni test or t-test. We found that behavioral sensitization was prevented and reversed by AT1-R blockade more efficiently than aripiprazole. Moreover, 5 days of CV administration increased DA uptake fact that could account for the behavioral results. We conclude that the lesser DA signaling, as a result of the increasing of its uptake, would explain the beneficial effects of AT1-R blockade in the behavioral neuroadaptations induced by AMPH.
Fil: Basmadjian, Osvaldo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Montemerlo, Antonella Evelin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Baiardi, Gustavo Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Rubianes, María Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Bregonzio, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Reunión Anual de Sociedades de Biociencias
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
Materia
AT1 receptors
Dopamine
Amphetamine
Schizophrenia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/154017
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/154017
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling AT1 receptors in striatum DA-uptake: Crucial role and relevance in an Amphetamine-sensitization model of SchizophreniaBasmadjian, Osvaldo MartinMontemerlo, Antonella EvelinRivas, Gustavo AdolfoBaiardi, Gustavo CarlosRubianes, María DoloresBregonzio, ClaudiaAT1 receptorsDopamineAmphetamineSchizophreniahttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Limbic dopamine (DA) hyperactivity, a hallmark of amphetamine (AMPH) exposure, it is considered as a neurochemical feature involved in the expression of schizophrenic positive symptoms. DA neurotransmission dynamics is regulated by the uptake of extracellular transmitter at presynaptic neurons through specific transporter. Angiotensin II, through AT1 receptors (AT1-R), modulates DA neurotransmission at limbic areas. Herein, we studied AT1-R involvement after AMPH exposure on: a) development and expression of behavioral sensitization, b) in vitro striatum DA uptake. To these purposes male Wistar rats (250-300 g) were daily administered with d-AMPH (2.5 mg/kg i.p.) for 5 days. After 3 weeks of withdrawal, the behavioral sensitization was evaluated measuring locomotor activity. The AT1-R blocker, Candesartan (CV 3 mg/kg p.o.), was administered daily for 10 days, starting 5 days prior to the first AMPH injection in the prevention sensitization protocol. In the reversion protocol, either, CV or aripiprazole (antipsychotic drug widely use partial agonist of D2 receptors), were administered for 5 days starting 2 weeks after the last AMPH injection. DA uptake was measured in homogenized striatum using an electrochemical sensor, based on glassy carbon electrode modified with carbon nanotubes and polyethylenimine by amperometry. The results were analyzed by 2-way ANOVA followed by Bonferroni test or t-test. We found that behavioral sensitization was prevented and reversed by AT1-R blockade more efficiently than aripiprazole. Moreover, 5 days of CV administration increased DA uptake fact that could account for the behavioral results. We conclude that the lesser DA signaling, as a result of the increasing of its uptake, would explain the beneficial effects of AT1-R blockade in the behavioral neuroadaptations induced by AMPH.Fil: Basmadjian, Osvaldo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; ArgentinaFil: Montemerlo, Antonella Evelin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Baiardi, Gustavo Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Rubianes, María Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Bregonzio, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaReunión Anual de Sociedades de BiocienciasMar del PlataArgentinaSociedad Argentina de Investigación ClínicaAsociación Argentina de Farmacología ExperimentalSociedad Argentina de BiologíaSociedad Argentina de ProtozoologíaAsociación Argentina de NanomedicinasFundación Revista MedicinaCostas, Monica AlejandraMarino, Gabriela InésAzurmendi, Pablo Javier2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/154017AT1 receptors in striatum DA-uptake: Crucial role and relevance in an Amphetamine-sensitization model of Schizophrenia; Reunión Anual de Sociedades de Biociencias; Mar del Plata; Argentina; 2019; 287-2870025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2010-a-2019/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:19:01Zoai:ri.conicet.gov.ar:11336/154017instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:19:01.724CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv AT1 receptors in striatum DA-uptake: Crucial role and relevance in an Amphetamine-sensitization model of Schizophrenia
title AT1 receptors in striatum DA-uptake: Crucial role and relevance in an Amphetamine-sensitization model of Schizophrenia
spellingShingle AT1 receptors in striatum DA-uptake: Crucial role and relevance in an Amphetamine-sensitization model of Schizophrenia
Basmadjian, Osvaldo Martin
AT1 receptors
Dopamine
Amphetamine
Schizophrenia
title_short AT1 receptors in striatum DA-uptake: Crucial role and relevance in an Amphetamine-sensitization model of Schizophrenia
title_full AT1 receptors in striatum DA-uptake: Crucial role and relevance in an Amphetamine-sensitization model of Schizophrenia
title_fullStr AT1 receptors in striatum DA-uptake: Crucial role and relevance in an Amphetamine-sensitization model of Schizophrenia
title_full_unstemmed AT1 receptors in striatum DA-uptake: Crucial role and relevance in an Amphetamine-sensitization model of Schizophrenia
title_sort AT1 receptors in striatum DA-uptake: Crucial role and relevance in an Amphetamine-sensitization model of Schizophrenia
dc.creator.none.fl_str_mv Basmadjian, Osvaldo Martin
Montemerlo, Antonella Evelin
Rivas, Gustavo Adolfo
Baiardi, Gustavo Carlos
Rubianes, María Dolores
Bregonzio, Claudia
author Basmadjian, Osvaldo Martin
author_facet Basmadjian, Osvaldo Martin
Montemerlo, Antonella Evelin
Rivas, Gustavo Adolfo
Baiardi, Gustavo Carlos
Rubianes, María Dolores
Bregonzio, Claudia
author_role author
author2 Montemerlo, Antonella Evelin
Rivas, Gustavo Adolfo
Baiardi, Gustavo Carlos
Rubianes, María Dolores
Bregonzio, Claudia
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Costas, Monica Alejandra
Marino, Gabriela Inés
Azurmendi, Pablo Javier
dc.subject.none.fl_str_mv AT1 receptors
Dopamine
Amphetamine
Schizophrenia
topic AT1 receptors
Dopamine
Amphetamine
Schizophrenia
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Limbic dopamine (DA) hyperactivity, a hallmark of amphetamine (AMPH) exposure, it is considered as a neurochemical feature involved in the expression of schizophrenic positive symptoms. DA neurotransmission dynamics is regulated by the uptake of extracellular transmitter at presynaptic neurons through specific transporter. Angiotensin II, through AT1 receptors (AT1-R), modulates DA neurotransmission at limbic areas. Herein, we studied AT1-R involvement after AMPH exposure on: a) development and expression of behavioral sensitization, b) in vitro striatum DA uptake. To these purposes male Wistar rats (250-300 g) were daily administered with d-AMPH (2.5 mg/kg i.p.) for 5 days. After 3 weeks of withdrawal, the behavioral sensitization was evaluated measuring locomotor activity. The AT1-R blocker, Candesartan (CV 3 mg/kg p.o.), was administered daily for 10 days, starting 5 days prior to the first AMPH injection in the prevention sensitization protocol. In the reversion protocol, either, CV or aripiprazole (antipsychotic drug widely use partial agonist of D2 receptors), were administered for 5 days starting 2 weeks after the last AMPH injection. DA uptake was measured in homogenized striatum using an electrochemical sensor, based on glassy carbon electrode modified with carbon nanotubes and polyethylenimine by amperometry. The results were analyzed by 2-way ANOVA followed by Bonferroni test or t-test. We found that behavioral sensitization was prevented and reversed by AT1-R blockade more efficiently than aripiprazole. Moreover, 5 days of CV administration increased DA uptake fact that could account for the behavioral results. We conclude that the lesser DA signaling, as a result of the increasing of its uptake, would explain the beneficial effects of AT1-R blockade in the behavioral neuroadaptations induced by AMPH.
Fil: Basmadjian, Osvaldo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Montemerlo, Antonella Evelin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Baiardi, Gustavo Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Rubianes, María Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Bregonzio, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Reunión Anual de Sociedades de Biociencias
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
description Limbic dopamine (DA) hyperactivity, a hallmark of amphetamine (AMPH) exposure, it is considered as a neurochemical feature involved in the expression of schizophrenic positive symptoms. DA neurotransmission dynamics is regulated by the uptake of extracellular transmitter at presynaptic neurons through specific transporter. Angiotensin II, through AT1 receptors (AT1-R), modulates DA neurotransmission at limbic areas. Herein, we studied AT1-R involvement after AMPH exposure on: a) development and expression of behavioral sensitization, b) in vitro striatum DA uptake. To these purposes male Wistar rats (250-300 g) were daily administered with d-AMPH (2.5 mg/kg i.p.) for 5 days. After 3 weeks of withdrawal, the behavioral sensitization was evaluated measuring locomotor activity. The AT1-R blocker, Candesartan (CV 3 mg/kg p.o.), was administered daily for 10 days, starting 5 days prior to the first AMPH injection in the prevention sensitization protocol. In the reversion protocol, either, CV or aripiprazole (antipsychotic drug widely use partial agonist of D2 receptors), were administered for 5 days starting 2 weeks after the last AMPH injection. DA uptake was measured in homogenized striatum using an electrochemical sensor, based on glassy carbon electrode modified with carbon nanotubes and polyethylenimine by amperometry. The results were analyzed by 2-way ANOVA followed by Bonferroni test or t-test. We found that behavioral sensitization was prevented and reversed by AT1-R blockade more efficiently than aripiprazole. Moreover, 5 days of CV administration increased DA uptake fact that could account for the behavioral results. We conclude that the lesser DA signaling, as a result of the increasing of its uptake, would explain the beneficial effects of AT1-R blockade in the behavioral neuroadaptations induced by AMPH.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/154017
AT1 receptors in striatum DA-uptake: Crucial role and relevance in an Amphetamine-sensitization model of Schizophrenia; Reunión Anual de Sociedades de Biociencias; Mar del Plata; Argentina; 2019; 287-287
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/154017
identifier_str_mv AT1 receptors in striatum DA-uptake: Crucial role and relevance in an Amphetamine-sensitization model of Schizophrenia; Reunión Anual de Sociedades de Biociencias; Mar del Plata; Argentina; 2019; 287-287
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2010-a-2019/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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