Wnt7b signalling through Frizzled-7 receptor promotes dendrite development by coactivating CaMKII and JNK

Autores
Ferrari, María Edith; Bernis, Maria Eugenia; McLeod, Faye; Podpolny, Marina; Coullery, Romina Paola; Casadei, Inelia Mailín Iara; Salinas, Patricia; Rosso, Silvana Beatriz
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The formation of complex dendritic arbors is crucial for the assembly of functional networks as abnormal dendrite formation underlies several neurodevelopmental and psychiatric disorders. Many extracellular factors have been postulated as regulators of dendritic growth. Wnt proteins play a critical role in neuronal development and circuit formation. We previously demonstrated that Wnt7b acts through the scaffold protein dishevelled 1 (Dvl1) to modulate dendrite arborisation by activating a non-canonical Wnt signalling pathway. Here,we identify the seven-transmembrane frizzled-7 (Fz7, also known as FZD7) as the receptor for Wnt7b-mediated dendrite growth and complexity. Importantly, Fz7 is developmentally regulated in the intact hippocampus, and is localised along neurites and at dendritic growth cones, suggesting a role in dendrite formation andmaturation. Fz7 lossof- function studies demonstrated that Wnt7b requires Fz7 to promote dendritic arborisation. Moreover, in vivo Fz7 loss of function results in dendritic defects in the intact mouse hippocampus. Furthermore, our findings reveal that Wnt7b and Fz7 induce the phosphorylation of Ca2 +/calmodulin-dependent protein kinase II (CaMKII) and JNK proteins, which are required for dendritic development. Here, we demonstrate that Wnt7b-Fz7 signals through two non-canonical Wnt pathways to modulate dendritic growth and complexity.
Fil: Ferrari, María Edith. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Toxicología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Bernis, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: McLeod, Faye. Colegio Universitario de Londres; Reino Unido
Fil: Podpolny, Marina. University College London; Estados Unidos
Fil: Coullery, Romina Paola. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Toxicología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Casadei, Inelia Mailín Iara. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Toxicología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Salinas, Patricia. Colegio Universitario de Londres; Reino Unido
Fil: Rosso, Silvana Beatriz. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Toxicología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Materia
CAMKII
DENDRITE DEVELOPMENT
FZ RECEPTOR
JNK
NEURON
WNT SIGNALLING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/178755
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/178755
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Wnt7b signalling through Frizzled-7 receptor promotes dendrite development by coactivating CaMKII and JNKFerrari, María EdithBernis, Maria EugeniaMcLeod, FayePodpolny, MarinaCoullery, Romina PaolaCasadei, Inelia Mailín IaraSalinas, PatriciaRosso, Silvana BeatrizCAMKIIDENDRITE DEVELOPMENTFZ RECEPTORJNKNEURONWNT SIGNALLINGhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The formation of complex dendritic arbors is crucial for the assembly of functional networks as abnormal dendrite formation underlies several neurodevelopmental and psychiatric disorders. Many extracellular factors have been postulated as regulators of dendritic growth. Wnt proteins play a critical role in neuronal development and circuit formation. We previously demonstrated that Wnt7b acts through the scaffold protein dishevelled 1 (Dvl1) to modulate dendrite arborisation by activating a non-canonical Wnt signalling pathway. Here,we identify the seven-transmembrane frizzled-7 (Fz7, also known as FZD7) as the receptor for Wnt7b-mediated dendrite growth and complexity. Importantly, Fz7 is developmentally regulated in the intact hippocampus, and is localised along neurites and at dendritic growth cones, suggesting a role in dendrite formation andmaturation. Fz7 lossof- function studies demonstrated that Wnt7b requires Fz7 to promote dendritic arborisation. Moreover, in vivo Fz7 loss of function results in dendritic defects in the intact mouse hippocampus. Furthermore, our findings reveal that Wnt7b and Fz7 induce the phosphorylation of Ca2 +/calmodulin-dependent protein kinase II (CaMKII) and JNK proteins, which are required for dendritic development. Here, we demonstrate that Wnt7b-Fz7 signals through two non-canonical Wnt pathways to modulate dendritic growth and complexity.Fil: Ferrari, María Edith. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Toxicología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Bernis, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: McLeod, Faye. Colegio Universitario de Londres; Reino UnidoFil: Podpolny, Marina. University College London; Estados UnidosFil: Coullery, Romina Paola. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Toxicología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Casadei, Inelia Mailín Iara. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Toxicología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Salinas, Patricia. Colegio Universitario de Londres; Reino UnidoFil: Rosso, Silvana Beatriz. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Toxicología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaCompany of Biologists2018-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/178755Ferrari, María Edith; Bernis, Maria Eugenia; McLeod, Faye; Podpolny, Marina; Coullery, Romina Paola; et al.; Wnt7b signalling through Frizzled-7 receptor promotes dendrite development by coactivating CaMKII and JNK; Company of Biologists; Journal of Cell Science; 131; 13; 6-2018; 1-410021-9533CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://jcs.biologists.orginfo:eu-repo/semantics/altIdentifier/doi/10.1242/jcs.216101info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:35:33Zoai:ri.conicet.gov.ar:11336/178755instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:35:33.403CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Wnt7b signalling through Frizzled-7 receptor promotes dendrite development by coactivating CaMKII and JNK
title Wnt7b signalling through Frizzled-7 receptor promotes dendrite development by coactivating CaMKII and JNK
spellingShingle Wnt7b signalling through Frizzled-7 receptor promotes dendrite development by coactivating CaMKII and JNK
Ferrari, María Edith
CAMKII
DENDRITE DEVELOPMENT
FZ RECEPTOR
JNK
NEURON
WNT SIGNALLING
title_short Wnt7b signalling through Frizzled-7 receptor promotes dendrite development by coactivating CaMKII and JNK
title_full Wnt7b signalling through Frizzled-7 receptor promotes dendrite development by coactivating CaMKII and JNK
title_fullStr Wnt7b signalling through Frizzled-7 receptor promotes dendrite development by coactivating CaMKII and JNK
title_full_unstemmed Wnt7b signalling through Frizzled-7 receptor promotes dendrite development by coactivating CaMKII and JNK
title_sort Wnt7b signalling through Frizzled-7 receptor promotes dendrite development by coactivating CaMKII and JNK
dc.creator.none.fl_str_mv Ferrari, María Edith
Bernis, Maria Eugenia
McLeod, Faye
Podpolny, Marina
Coullery, Romina Paola
Casadei, Inelia Mailín Iara
Salinas, Patricia
Rosso, Silvana Beatriz
author Ferrari, María Edith
author_facet Ferrari, María Edith
Bernis, Maria Eugenia
McLeod, Faye
Podpolny, Marina
Coullery, Romina Paola
Casadei, Inelia Mailín Iara
Salinas, Patricia
Rosso, Silvana Beatriz
author_role author
author2 Bernis, Maria Eugenia
McLeod, Faye
Podpolny, Marina
Coullery, Romina Paola
Casadei, Inelia Mailín Iara
Salinas, Patricia
Rosso, Silvana Beatriz
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CAMKII
DENDRITE DEVELOPMENT
FZ RECEPTOR
JNK
NEURON
WNT SIGNALLING
topic CAMKII
DENDRITE DEVELOPMENT
FZ RECEPTOR
JNK
NEURON
WNT SIGNALLING
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The formation of complex dendritic arbors is crucial for the assembly of functional networks as abnormal dendrite formation underlies several neurodevelopmental and psychiatric disorders. Many extracellular factors have been postulated as regulators of dendritic growth. Wnt proteins play a critical role in neuronal development and circuit formation. We previously demonstrated that Wnt7b acts through the scaffold protein dishevelled 1 (Dvl1) to modulate dendrite arborisation by activating a non-canonical Wnt signalling pathway. Here,we identify the seven-transmembrane frizzled-7 (Fz7, also known as FZD7) as the receptor for Wnt7b-mediated dendrite growth and complexity. Importantly, Fz7 is developmentally regulated in the intact hippocampus, and is localised along neurites and at dendritic growth cones, suggesting a role in dendrite formation andmaturation. Fz7 lossof- function studies demonstrated that Wnt7b requires Fz7 to promote dendritic arborisation. Moreover, in vivo Fz7 loss of function results in dendritic defects in the intact mouse hippocampus. Furthermore, our findings reveal that Wnt7b and Fz7 induce the phosphorylation of Ca2 +/calmodulin-dependent protein kinase II (CaMKII) and JNK proteins, which are required for dendritic development. Here, we demonstrate that Wnt7b-Fz7 signals through two non-canonical Wnt pathways to modulate dendritic growth and complexity.
Fil: Ferrari, María Edith. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Toxicología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Bernis, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: McLeod, Faye. Colegio Universitario de Londres; Reino Unido
Fil: Podpolny, Marina. University College London; Estados Unidos
Fil: Coullery, Romina Paola. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Toxicología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Casadei, Inelia Mailín Iara. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Toxicología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Salinas, Patricia. Colegio Universitario de Londres; Reino Unido
Fil: Rosso, Silvana Beatriz. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Toxicología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
description The formation of complex dendritic arbors is crucial for the assembly of functional networks as abnormal dendrite formation underlies several neurodevelopmental and psychiatric disorders. Many extracellular factors have been postulated as regulators of dendritic growth. Wnt proteins play a critical role in neuronal development and circuit formation. We previously demonstrated that Wnt7b acts through the scaffold protein dishevelled 1 (Dvl1) to modulate dendrite arborisation by activating a non-canonical Wnt signalling pathway. Here,we identify the seven-transmembrane frizzled-7 (Fz7, also known as FZD7) as the receptor for Wnt7b-mediated dendrite growth and complexity. Importantly, Fz7 is developmentally regulated in the intact hippocampus, and is localised along neurites and at dendritic growth cones, suggesting a role in dendrite formation andmaturation. Fz7 lossof- function studies demonstrated that Wnt7b requires Fz7 to promote dendritic arborisation. Moreover, in vivo Fz7 loss of function results in dendritic defects in the intact mouse hippocampus. Furthermore, our findings reveal that Wnt7b and Fz7 induce the phosphorylation of Ca2 +/calmodulin-dependent protein kinase II (CaMKII) and JNK proteins, which are required for dendritic development. Here, we demonstrate that Wnt7b-Fz7 signals through two non-canonical Wnt pathways to modulate dendritic growth and complexity.
publishDate 2018
dc.date.none.fl_str_mv 2018-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/178755
Ferrari, María Edith; Bernis, Maria Eugenia; McLeod, Faye; Podpolny, Marina; Coullery, Romina Paola; et al.; Wnt7b signalling through Frizzled-7 receptor promotes dendrite development by coactivating CaMKII and JNK; Company of Biologists; Journal of Cell Science; 131; 13; 6-2018; 1-41
0021-9533
CONICET Digital
CONICET
url http://hdl.handle.net/11336/178755
identifier_str_mv Ferrari, María Edith; Bernis, Maria Eugenia; McLeod, Faye; Podpolny, Marina; Coullery, Romina Paola; et al.; Wnt7b signalling through Frizzled-7 receptor promotes dendrite development by coactivating CaMKII and JNK; Company of Biologists; Journal of Cell Science; 131; 13; 6-2018; 1-41
0021-9533
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://jcs.biologists.org
info:eu-repo/semantics/altIdentifier/doi/10.1242/jcs.216101
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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