Effects of isolated or enriched housing at adolescence upon ethanol intake and anxiety responses, in rats exposed to prenatal ethanol

Autores
Di Doi, Paula Luciana; Fernandez, Macarena Soledad; Haeger Soto, Paola; Pautassi, Ricardo Marcos
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Prenatal alcohol exposure (PAE) is highly prevalent worldwide, and can affect alcohol intake and anxiety responses in the offspring. It is still relatively unknown how PAE interacts with stress exposure at adolescence, and there is a scarcity of treatments to reduce the impact of PAE. The present study assessed alcohol (ethanol) intake and anxiety responses after PAE and tested the modulation of these responses by different housing conditions during early adolescence. Pregnant dams were exposed for 22 hours/day, from gestational day 0, throughout pregnancy, and until postnatal day 7 (PD 7), to a single bottle of 10% ethanol that was mixed in tap water and sucralose (EtOH Group), or to a single bottle of tap water and sucralose (CTRL Group). During PDs 21-42 the offspring was exposed to standard pair housing, isolated housing or enriched housing. Immediately after, they were pair-housed and tested for ethanol intake in 24-hour access, intermittent 2-bottle choice sessions conducted over 4 weeks, and for anxiety responses in the light-dark box test. The EtOH offspring exhibited heightened anxiety (i.e., greater avoidance of the white area of the light-dark box) at PD21, when compared to control (CTRL) rats. Isolated housing induced greater avoidance of the white area, when compared to standard or enriched housing, on PDs 42 and 70. There were no significant EtOH versus CTRL differences in relative (i.e., percent preference vs. water) ethanol intake, yet overall fluid intake and absolute (g/kg) ethanol intake were significantly lower in EtOH versus CTRL rats. Enriched housing during adolescence had a suppressing effect upon absolute ethanol intake during the first week of testing, an effect more noticeable in CTRL than in PAE rats. The present study indicates that PAE or isolated housing is associated with an anxiety-like phenotype. The protracted PAE protocol here employed caused a generalized reduction of intake behaviours. Environmental enrichment was associated with a subtle, yet significant, reduction in absolute ethanol intake.
Fil: Di Doi, Paula Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Fernandez, Macarena Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; Argentina
Fil: Haeger Soto, Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Pautassi, Ricardo Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Materia
Ethanol
Adolescence
Prenatal ethanol
Intake
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/128586
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of isolated or enriched housing at adolescence upon ethanol intake and anxiety responses, in rats exposed to prenatal ethanolDi Doi, Paula LucianaFernandez, Macarena SoledadHaeger Soto, PaolaPautassi, Ricardo MarcosEthanolAdolescencePrenatal ethanolIntakehttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Prenatal alcohol exposure (PAE) is highly prevalent worldwide, and can affect alcohol intake and anxiety responses in the offspring. It is still relatively unknown how PAE interacts with stress exposure at adolescence, and there is a scarcity of treatments to reduce the impact of PAE. The present study assessed alcohol (ethanol) intake and anxiety responses after PAE and tested the modulation of these responses by different housing conditions during early adolescence. Pregnant dams were exposed for 22 hours/day, from gestational day 0, throughout pregnancy, and until postnatal day 7 (PD 7), to a single bottle of 10% ethanol that was mixed in tap water and sucralose (EtOH Group), or to a single bottle of tap water and sucralose (CTRL Group). During PDs 21-42 the offspring was exposed to standard pair housing, isolated housing or enriched housing. Immediately after, they were pair-housed and tested for ethanol intake in 24-hour access, intermittent 2-bottle choice sessions conducted over 4 weeks, and for anxiety responses in the light-dark box test. The EtOH offspring exhibited heightened anxiety (i.e., greater avoidance of the white area of the light-dark box) at PD21, when compared to control (CTRL) rats. Isolated housing induced greater avoidance of the white area, when compared to standard or enriched housing, on PDs 42 and 70. There were no significant EtOH versus CTRL differences in relative (i.e., percent preference vs. water) ethanol intake, yet overall fluid intake and absolute (g/kg) ethanol intake were significantly lower in EtOH versus CTRL rats. Enriched housing during adolescence had a suppressing effect upon absolute ethanol intake during the first week of testing, an effect more noticeable in CTRL than in PAE rats. The present study indicates that PAE or isolated housing is associated with an anxiety-like phenotype. The protracted PAE protocol here employed caused a generalized reduction of intake behaviours. Environmental enrichment was associated with a subtle, yet significant, reduction in absolute ethanol intake.Fil: Di Doi, Paula Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Fernandez, Macarena Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; ArgentinaFil: Haeger Soto, Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Pautassi, Ricardo Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaDougmar Press2019-01-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/128586Di Doi, Paula Luciana; Fernandez, Macarena Soledad; Haeger Soto, Paola; Pautassi, Ricardo Marcos; Effects of isolated or enriched housing at adolescence upon ethanol intake and anxiety responses, in rats exposed to prenatal ethanol; Dougmar Press; Journal of Fetal Alcohol Spectrum Risk and Prevention; 2019; 22-1-2019; 1-122561-9187CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://jfasrp.com/index.php/JFASRP/article/view/3info:eu-repo/semantics/altIdentifier/doi/10.22374/jfasrp.v2i1.3info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:15:55Zoai:ri.conicet.gov.ar:11336/128586instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:15:55.867CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of isolated or enriched housing at adolescence upon ethanol intake and anxiety responses, in rats exposed to prenatal ethanol
title Effects of isolated or enriched housing at adolescence upon ethanol intake and anxiety responses, in rats exposed to prenatal ethanol
spellingShingle Effects of isolated or enriched housing at adolescence upon ethanol intake and anxiety responses, in rats exposed to prenatal ethanol
Di Doi, Paula Luciana
Ethanol
Adolescence
Prenatal ethanol
Intake
title_short Effects of isolated or enriched housing at adolescence upon ethanol intake and anxiety responses, in rats exposed to prenatal ethanol
title_full Effects of isolated or enriched housing at adolescence upon ethanol intake and anxiety responses, in rats exposed to prenatal ethanol
title_fullStr Effects of isolated or enriched housing at adolescence upon ethanol intake and anxiety responses, in rats exposed to prenatal ethanol
title_full_unstemmed Effects of isolated or enriched housing at adolescence upon ethanol intake and anxiety responses, in rats exposed to prenatal ethanol
title_sort Effects of isolated or enriched housing at adolescence upon ethanol intake and anxiety responses, in rats exposed to prenatal ethanol
dc.creator.none.fl_str_mv Di Doi, Paula Luciana
Fernandez, Macarena Soledad
Haeger Soto, Paola
Pautassi, Ricardo Marcos
author Di Doi, Paula Luciana
author_facet Di Doi, Paula Luciana
Fernandez, Macarena Soledad
Haeger Soto, Paola
Pautassi, Ricardo Marcos
author_role author
author2 Fernandez, Macarena Soledad
Haeger Soto, Paola
Pautassi, Ricardo Marcos
author2_role author
author
author
dc.subject.none.fl_str_mv Ethanol
Adolescence
Prenatal ethanol
Intake
topic Ethanol
Adolescence
Prenatal ethanol
Intake
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Prenatal alcohol exposure (PAE) is highly prevalent worldwide, and can affect alcohol intake and anxiety responses in the offspring. It is still relatively unknown how PAE interacts with stress exposure at adolescence, and there is a scarcity of treatments to reduce the impact of PAE. The present study assessed alcohol (ethanol) intake and anxiety responses after PAE and tested the modulation of these responses by different housing conditions during early adolescence. Pregnant dams were exposed for 22 hours/day, from gestational day 0, throughout pregnancy, and until postnatal day 7 (PD 7), to a single bottle of 10% ethanol that was mixed in tap water and sucralose (EtOH Group), or to a single bottle of tap water and sucralose (CTRL Group). During PDs 21-42 the offspring was exposed to standard pair housing, isolated housing or enriched housing. Immediately after, they were pair-housed and tested for ethanol intake in 24-hour access, intermittent 2-bottle choice sessions conducted over 4 weeks, and for anxiety responses in the light-dark box test. The EtOH offspring exhibited heightened anxiety (i.e., greater avoidance of the white area of the light-dark box) at PD21, when compared to control (CTRL) rats. Isolated housing induced greater avoidance of the white area, when compared to standard or enriched housing, on PDs 42 and 70. There were no significant EtOH versus CTRL differences in relative (i.e., percent preference vs. water) ethanol intake, yet overall fluid intake and absolute (g/kg) ethanol intake were significantly lower in EtOH versus CTRL rats. Enriched housing during adolescence had a suppressing effect upon absolute ethanol intake during the first week of testing, an effect more noticeable in CTRL than in PAE rats. The present study indicates that PAE or isolated housing is associated with an anxiety-like phenotype. The protracted PAE protocol here employed caused a generalized reduction of intake behaviours. Environmental enrichment was associated with a subtle, yet significant, reduction in absolute ethanol intake.
Fil: Di Doi, Paula Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Fernandez, Macarena Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; Argentina
Fil: Haeger Soto, Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Pautassi, Ricardo Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
description Prenatal alcohol exposure (PAE) is highly prevalent worldwide, and can affect alcohol intake and anxiety responses in the offspring. It is still relatively unknown how PAE interacts with stress exposure at adolescence, and there is a scarcity of treatments to reduce the impact of PAE. The present study assessed alcohol (ethanol) intake and anxiety responses after PAE and tested the modulation of these responses by different housing conditions during early adolescence. Pregnant dams were exposed for 22 hours/day, from gestational day 0, throughout pregnancy, and until postnatal day 7 (PD 7), to a single bottle of 10% ethanol that was mixed in tap water and sucralose (EtOH Group), or to a single bottle of tap water and sucralose (CTRL Group). During PDs 21-42 the offspring was exposed to standard pair housing, isolated housing or enriched housing. Immediately after, they were pair-housed and tested for ethanol intake in 24-hour access, intermittent 2-bottle choice sessions conducted over 4 weeks, and for anxiety responses in the light-dark box test. The EtOH offspring exhibited heightened anxiety (i.e., greater avoidance of the white area of the light-dark box) at PD21, when compared to control (CTRL) rats. Isolated housing induced greater avoidance of the white area, when compared to standard or enriched housing, on PDs 42 and 70. There were no significant EtOH versus CTRL differences in relative (i.e., percent preference vs. water) ethanol intake, yet overall fluid intake and absolute (g/kg) ethanol intake were significantly lower in EtOH versus CTRL rats. Enriched housing during adolescence had a suppressing effect upon absolute ethanol intake during the first week of testing, an effect more noticeable in CTRL than in PAE rats. The present study indicates that PAE or isolated housing is associated with an anxiety-like phenotype. The protracted PAE protocol here employed caused a generalized reduction of intake behaviours. Environmental enrichment was associated with a subtle, yet significant, reduction in absolute ethanol intake.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-22
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/128586
Di Doi, Paula Luciana; Fernandez, Macarena Soledad; Haeger Soto, Paola; Pautassi, Ricardo Marcos; Effects of isolated or enriched housing at adolescence upon ethanol intake and anxiety responses, in rats exposed to prenatal ethanol; Dougmar Press; Journal of Fetal Alcohol Spectrum Risk and Prevention; 2019; 22-1-2019; 1-12
2561-9187
CONICET Digital
CONICET
url http://hdl.handle.net/11336/128586
identifier_str_mv Di Doi, Paula Luciana; Fernandez, Macarena Soledad; Haeger Soto, Paola; Pautassi, Ricardo Marcos; Effects of isolated or enriched housing at adolescence upon ethanol intake and anxiety responses, in rats exposed to prenatal ethanol; Dougmar Press; Journal of Fetal Alcohol Spectrum Risk and Prevention; 2019; 22-1-2019; 1-12
2561-9187
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://jfasrp.com/index.php/JFASRP/article/view/3
info:eu-repo/semantics/altIdentifier/doi/10.22374/jfasrp.v2i1.3
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Dougmar Press
publisher.none.fl_str_mv Dougmar Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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