Arabidopsis HAP2/GCS1 is a gamete fusion protein homologous to somatic and viral fusogens

Autores
Valansi, Clari; Moi, David; Leikina, Evgenia; Matveev, Elena; Graña, Martín; Chernomordik, Leonid V.; Romero, Héctor; Aguilar, Pablo Sebastián; Podbilewicz, Benjamin
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cell-cell fusion is inherent to sexual reproduction. Loss of HAP LESS 2/GEN ERA TIVE CELL SPE CIF IC 1 (HAP2/GCS1) proteins results in gamete fusion failure in diverse organisms, but their exact role is unclear. In this study, we show that Arabidopsis thaliana HAP2/GCS1 is sufficient to promote mammalian cell-cell fusion. Hemifusion and complete fusion depend on HAP2/GCS1 presence in both fusing cells. Furthermore, expression of HAP2 on the surface of pseudotyped vesicular stomatitis virus results in homotypic virus-cell fusion. We demonstrate that the Caenorhabditis elegans Epithelial Fusion Failure 1 (EFF-1) somatic cell fusogen can replace HAP2/GCS1 in one of the fusing membranes, indicating that HAP2/GCS1 and EFF-1 share a similar fusion mechanism. Structural modeling of the HAP2/GCS1 protein family predicts that they are homologous to EFF-1 and viral class II fusion proteins (e.g., Zika virus). We name this superfamily Fusexins: fusion proteins essential for sexual reproduction and exoplasmic merger of plasma membranes. We suggest a common origin and evolution of sexual reproduction, enveloped virus entry into cells, and somatic cell fusion.
Fil: Valansi, Clari. Technion- Israel Institute of Technology; Israel
Fil: Moi, David. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Leikina, Evgenia. National Institutes of Health; Estados Unidos
Fil: Matveev, Elena. Technion- Israel Institute of Technology; Israel
Fil: Graña, Martín. Instituto Pasteur de Montevideo; Uruguay
Fil: Chernomordik, Leonid V.. National Institutes of Health; Estados Unidos
Fil: Romero, Héctor. Universidad de la República; Uruguay
Fil: Aguilar, Pablo Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Podbilewicz, Benjamin. Technion- Israel Institute of Technology; Israel
Materia
FUSION
FERTILIZATION
VIRAL
GAMETE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/53425
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/53425
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Arabidopsis HAP2/GCS1 is a gamete fusion protein homologous to somatic and viral fusogensValansi, ClariMoi, DavidLeikina, EvgeniaMatveev, ElenaGraña, MartínChernomordik, Leonid V.Romero, HéctorAguilar, Pablo SebastiánPodbilewicz, BenjaminFUSIONFERTILIZATIONVIRALGAMETEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cell-cell fusion is inherent to sexual reproduction. Loss of HAP LESS 2/GEN ERA TIVE CELL SPE CIF IC 1 (HAP2/GCS1) proteins results in gamete fusion failure in diverse organisms, but their exact role is unclear. In this study, we show that Arabidopsis thaliana HAP2/GCS1 is sufficient to promote mammalian cell-cell fusion. Hemifusion and complete fusion depend on HAP2/GCS1 presence in both fusing cells. Furthermore, expression of HAP2 on the surface of pseudotyped vesicular stomatitis virus results in homotypic virus-cell fusion. We demonstrate that the Caenorhabditis elegans Epithelial Fusion Failure 1 (EFF-1) somatic cell fusogen can replace HAP2/GCS1 in one of the fusing membranes, indicating that HAP2/GCS1 and EFF-1 share a similar fusion mechanism. Structural modeling of the HAP2/GCS1 protein family predicts that they are homologous to EFF-1 and viral class II fusion proteins (e.g., Zika virus). We name this superfamily Fusexins: fusion proteins essential for sexual reproduction and exoplasmic merger of plasma membranes. We suggest a common origin and evolution of sexual reproduction, enveloped virus entry into cells, and somatic cell fusion.Fil: Valansi, Clari. Technion- Israel Institute of Technology; IsraelFil: Moi, David. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Leikina, Evgenia. National Institutes of Health; Estados UnidosFil: Matveev, Elena. Technion- Israel Institute of Technology; IsraelFil: Graña, Martín. Instituto Pasteur de Montevideo; UruguayFil: Chernomordik, Leonid V.. National Institutes of Health; Estados UnidosFil: Romero, Héctor. Universidad de la República; UruguayFil: Aguilar, Pablo Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Podbilewicz, Benjamin. Technion- Israel Institute of Technology; IsraelRockefeller University Press2017-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53425Valansi, Clari; Moi, David; Leikina, Evgenia; Matveev, Elena; Graña, Martín; et al.; Arabidopsis HAP2/GCS1 is a gamete fusion protein homologous to somatic and viral fusogens; Rockefeller University Press; Journal of Cell Biology; 216; 3; 1-2017; 1-110021-9525CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1083/jcb.201610093info:eu-repo/semantics/altIdentifier/url/http://jcb.rupress.org/content/216/3/571info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:50:48Zoai:ri.conicet.gov.ar:11336/53425instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:50:48.317CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Arabidopsis HAP2/GCS1 is a gamete fusion protein homologous to somatic and viral fusogens
title Arabidopsis HAP2/GCS1 is a gamete fusion protein homologous to somatic and viral fusogens
spellingShingle Arabidopsis HAP2/GCS1 is a gamete fusion protein homologous to somatic and viral fusogens
Valansi, Clari
FUSION
FERTILIZATION
VIRAL
GAMETE
title_short Arabidopsis HAP2/GCS1 is a gamete fusion protein homologous to somatic and viral fusogens
title_full Arabidopsis HAP2/GCS1 is a gamete fusion protein homologous to somatic and viral fusogens
title_fullStr Arabidopsis HAP2/GCS1 is a gamete fusion protein homologous to somatic and viral fusogens
title_full_unstemmed Arabidopsis HAP2/GCS1 is a gamete fusion protein homologous to somatic and viral fusogens
title_sort Arabidopsis HAP2/GCS1 is a gamete fusion protein homologous to somatic and viral fusogens
dc.creator.none.fl_str_mv Valansi, Clari
Moi, David
Leikina, Evgenia
Matveev, Elena
Graña, Martín
Chernomordik, Leonid V.
Romero, Héctor
Aguilar, Pablo Sebastián
Podbilewicz, Benjamin
author Valansi, Clari
author_facet Valansi, Clari
Moi, David
Leikina, Evgenia
Matveev, Elena
Graña, Martín
Chernomordik, Leonid V.
Romero, Héctor
Aguilar, Pablo Sebastián
Podbilewicz, Benjamin
author_role author
author2 Moi, David
Leikina, Evgenia
Matveev, Elena
Graña, Martín
Chernomordik, Leonid V.
Romero, Héctor
Aguilar, Pablo Sebastián
Podbilewicz, Benjamin
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FUSION
FERTILIZATION
VIRAL
GAMETE
topic FUSION
FERTILIZATION
VIRAL
GAMETE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cell-cell fusion is inherent to sexual reproduction. Loss of HAP LESS 2/GEN ERA TIVE CELL SPE CIF IC 1 (HAP2/GCS1) proteins results in gamete fusion failure in diverse organisms, but their exact role is unclear. In this study, we show that Arabidopsis thaliana HAP2/GCS1 is sufficient to promote mammalian cell-cell fusion. Hemifusion and complete fusion depend on HAP2/GCS1 presence in both fusing cells. Furthermore, expression of HAP2 on the surface of pseudotyped vesicular stomatitis virus results in homotypic virus-cell fusion. We demonstrate that the Caenorhabditis elegans Epithelial Fusion Failure 1 (EFF-1) somatic cell fusogen can replace HAP2/GCS1 in one of the fusing membranes, indicating that HAP2/GCS1 and EFF-1 share a similar fusion mechanism. Structural modeling of the HAP2/GCS1 protein family predicts that they are homologous to EFF-1 and viral class II fusion proteins (e.g., Zika virus). We name this superfamily Fusexins: fusion proteins essential for sexual reproduction and exoplasmic merger of plasma membranes. We suggest a common origin and evolution of sexual reproduction, enveloped virus entry into cells, and somatic cell fusion.
Fil: Valansi, Clari. Technion- Israel Institute of Technology; Israel
Fil: Moi, David. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Leikina, Evgenia. National Institutes of Health; Estados Unidos
Fil: Matveev, Elena. Technion- Israel Institute of Technology; Israel
Fil: Graña, Martín. Instituto Pasteur de Montevideo; Uruguay
Fil: Chernomordik, Leonid V.. National Institutes of Health; Estados Unidos
Fil: Romero, Héctor. Universidad de la República; Uruguay
Fil: Aguilar, Pablo Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Podbilewicz, Benjamin. Technion- Israel Institute of Technology; Israel
description Cell-cell fusion is inherent to sexual reproduction. Loss of HAP LESS 2/GEN ERA TIVE CELL SPE CIF IC 1 (HAP2/GCS1) proteins results in gamete fusion failure in diverse organisms, but their exact role is unclear. In this study, we show that Arabidopsis thaliana HAP2/GCS1 is sufficient to promote mammalian cell-cell fusion. Hemifusion and complete fusion depend on HAP2/GCS1 presence in both fusing cells. Furthermore, expression of HAP2 on the surface of pseudotyped vesicular stomatitis virus results in homotypic virus-cell fusion. We demonstrate that the Caenorhabditis elegans Epithelial Fusion Failure 1 (EFF-1) somatic cell fusogen can replace HAP2/GCS1 in one of the fusing membranes, indicating that HAP2/GCS1 and EFF-1 share a similar fusion mechanism. Structural modeling of the HAP2/GCS1 protein family predicts that they are homologous to EFF-1 and viral class II fusion proteins (e.g., Zika virus). We name this superfamily Fusexins: fusion proteins essential for sexual reproduction and exoplasmic merger of plasma membranes. We suggest a common origin and evolution of sexual reproduction, enveloped virus entry into cells, and somatic cell fusion.
publishDate 2017
dc.date.none.fl_str_mv 2017-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/53425
Valansi, Clari; Moi, David; Leikina, Evgenia; Matveev, Elena; Graña, Martín; et al.; Arabidopsis HAP2/GCS1 is a gamete fusion protein homologous to somatic and viral fusogens; Rockefeller University Press; Journal of Cell Biology; 216; 3; 1-2017; 1-11
0021-9525
CONICET Digital
CONICET
url http://hdl.handle.net/11336/53425
identifier_str_mv Valansi, Clari; Moi, David; Leikina, Evgenia; Matveev, Elena; Graña, Martín; et al.; Arabidopsis HAP2/GCS1 is a gamete fusion protein homologous to somatic and viral fusogens; Rockefeller University Press; Journal of Cell Biology; 216; 3; 1-2017; 1-11
0021-9525
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1083/jcb.201610093
info:eu-repo/semantics/altIdentifier/url/http://jcb.rupress.org/content/216/3/571
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Rockefeller University Press
publisher.none.fl_str_mv Rockefeller University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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