Multiple-Herbicide Resistance in a 2,4-D - Resistant Waterhemp (Amaranthus tuberculatus) Population from Nebraska

Autores
Crespo, Roberto Javier; Wingeyer, Ana Beatriz; Kruger, Greg R.; Riggins, Chance W.; Tranel, Patrick J.; Bernards, Mark L.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A 2,4-D-resistant tall waterhemp population (FS) from Nebraska was evaluated for resistance to other TIR1 auxin receptor herbicides and to herbicides having alternative mechanisms of action using greenhouse bioassays and genetic markers. Atrazine, imazethapyr, lactofen, mesotrione, glufosinate, and glyphosate were applied in a single-dose bioassay, and tissue was collected from marked plants for genetic analysis. The FS population was not injured by atrazine or by imazethapyr. Approximately 50% of the plants survived lactofen and were actively growing 28 d after treatment. The population was susceptible to mesotrione, glufosinate, and glyphosate. Ametryn, chlorimuron-ethyl, 2,4-D, aminocyclopyraclor, aminopyralid, and picloram were applied in dose - response studies. The FS population was sensitive to ametryn, and the Ser-264-Gly substitution in the D1 protein was not detected, suggesting the lack of response to atrazine is not due to a target-site mutation. The FS population exhibited less than 50% injury to chlorimuron-ethyl at application rates 20 times the labeled use rate. The Ser-653-Asn acetolactate synthase (ALS) substitution, which confers resistance to imidazolinone herbicides, was present in the FS population. However, this does not explain the lack of response to the sulfonylurea herbicide, chlorimuron-ethyl. Sequencing of a portion of the PPX2L gene did not show the ΔG210 mutation that confers resistance to protoporphyrinogen oxidase - inhibiting herbicides, suggesting that other factors were responsible for waterhemp survival after lactofen application. The FS population was confirmed to be at least 30-fold resistant to 2,4-D relative to the susceptible populations. In addition, it was at least 3-fold less sensitive to aminopyralid and picloram, two other TIR1 auxin receptor herbicides, than the 2,4-D-susceptible populations were. These data indicated that the FS population contains both target and non - target site mechanisms conferring resistance to herbicides spanning at least three mechanisms of action: TIR1 auxin receptors, ALS inhibitors, and photosystem II inhibitors.
Fil: Crespo, Roberto Javier. University of Nebraska; Estados Unidos
Fil: Wingeyer, Ana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Paraná; Argentina
Fil: Kruger, Greg R.. University of Nebraska; Estados Unidos
Fil: Riggins, Chance W.. University of Illinois at Urbana; Estados Unidos
Fil: Tranel, Patrick J.. University of Illinois at Urbana; Estados Unidos
Fil: Bernards, Mark L.. University of Nebraska; Estados Unidos
Materia
CROSS-RESISTANCE
DOSE - RESPONSE
HERBICIDE RESISTANCE
INJURY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/75217

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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Multiple-Herbicide Resistance in a 2,4-D - Resistant Waterhemp (Amaranthus tuberculatus) Population from NebraskaCrespo, Roberto JavierWingeyer, Ana BeatrizKruger, Greg R.Riggins, Chance W.Tranel, Patrick J.Bernards, Mark L.CROSS-RESISTANCEDOSE - RESPONSEHERBICIDE RESISTANCEINJURYhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4A 2,4-D-resistant tall waterhemp population (FS) from Nebraska was evaluated for resistance to other TIR1 auxin receptor herbicides and to herbicides having alternative mechanisms of action using greenhouse bioassays and genetic markers. Atrazine, imazethapyr, lactofen, mesotrione, glufosinate, and glyphosate were applied in a single-dose bioassay, and tissue was collected from marked plants for genetic analysis. The FS population was not injured by atrazine or by imazethapyr. Approximately 50% of the plants survived lactofen and were actively growing 28 d after treatment. The population was susceptible to mesotrione, glufosinate, and glyphosate. Ametryn, chlorimuron-ethyl, 2,4-D, aminocyclopyraclor, aminopyralid, and picloram were applied in dose - response studies. The FS population was sensitive to ametryn, and the Ser-264-Gly substitution in the D1 protein was not detected, suggesting the lack of response to atrazine is not due to a target-site mutation. The FS population exhibited less than 50% injury to chlorimuron-ethyl at application rates 20 times the labeled use rate. The Ser-653-Asn acetolactate synthase (ALS) substitution, which confers resistance to imidazolinone herbicides, was present in the FS population. However, this does not explain the lack of response to the sulfonylurea herbicide, chlorimuron-ethyl. Sequencing of a portion of the PPX2L gene did not show the ΔG210 mutation that confers resistance to protoporphyrinogen oxidase - inhibiting herbicides, suggesting that other factors were responsible for waterhemp survival after lactofen application. The FS population was confirmed to be at least 30-fold resistant to 2,4-D relative to the susceptible populations. In addition, it was at least 3-fold less sensitive to aminopyralid and picloram, two other TIR1 auxin receptor herbicides, than the 2,4-D-susceptible populations were. These data indicated that the FS population contains both target and non - target site mechanisms conferring resistance to herbicides spanning at least three mechanisms of action: TIR1 auxin receptors, ALS inhibitors, and photosystem II inhibitors.Fil: Crespo, Roberto Javier. University of Nebraska; Estados UnidosFil: Wingeyer, Ana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Paraná; ArgentinaFil: Kruger, Greg R.. University of Nebraska; Estados UnidosFil: Riggins, Chance W.. University of Illinois at Urbana; Estados UnidosFil: Tranel, Patrick J.. University of Illinois at Urbana; Estados UnidosFil: Bernards, Mark L.. University of Nebraska; Estados UnidosWeed Science Society of America2017-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/75217Crespo, Roberto Javier; Wingeyer, Ana Beatriz; Kruger, Greg R.; Riggins, Chance W.; Tranel, Patrick J.; et al.; Multiple-Herbicide Resistance in a 2,4-D - Resistant Waterhemp (Amaranthus tuberculatus) Population from Nebraska; Weed Science Society of America; Weed Science; 65; 6; 11-2017; 743-7540043-1745CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/weed-science/article/multipleherbicide-resistance-in-a-24dresistant-waterhemp-amaranthus-tuberculatus-population-from-nebraska/9309AB3614CF11C833C658CE65CA1114info:eu-repo/semantics/altIdentifier/doi/10.1017/wsc.2017.39info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:26:23Zoai:ri.conicet.gov.ar:11336/75217instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:26:24.176CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Multiple-Herbicide Resistance in a 2,4-D - Resistant Waterhemp (Amaranthus tuberculatus) Population from Nebraska
title Multiple-Herbicide Resistance in a 2,4-D - Resistant Waterhemp (Amaranthus tuberculatus) Population from Nebraska
spellingShingle Multiple-Herbicide Resistance in a 2,4-D - Resistant Waterhemp (Amaranthus tuberculatus) Population from Nebraska
Crespo, Roberto Javier
CROSS-RESISTANCE
DOSE - RESPONSE
HERBICIDE RESISTANCE
INJURY
title_short Multiple-Herbicide Resistance in a 2,4-D - Resistant Waterhemp (Amaranthus tuberculatus) Population from Nebraska
title_full Multiple-Herbicide Resistance in a 2,4-D - Resistant Waterhemp (Amaranthus tuberculatus) Population from Nebraska
title_fullStr Multiple-Herbicide Resistance in a 2,4-D - Resistant Waterhemp (Amaranthus tuberculatus) Population from Nebraska
title_full_unstemmed Multiple-Herbicide Resistance in a 2,4-D - Resistant Waterhemp (Amaranthus tuberculatus) Population from Nebraska
title_sort Multiple-Herbicide Resistance in a 2,4-D - Resistant Waterhemp (Amaranthus tuberculatus) Population from Nebraska
dc.creator.none.fl_str_mv Crespo, Roberto Javier
Wingeyer, Ana Beatriz
Kruger, Greg R.
Riggins, Chance W.
Tranel, Patrick J.
Bernards, Mark L.
author Crespo, Roberto Javier
author_facet Crespo, Roberto Javier
Wingeyer, Ana Beatriz
Kruger, Greg R.
Riggins, Chance W.
Tranel, Patrick J.
Bernards, Mark L.
author_role author
author2 Wingeyer, Ana Beatriz
Kruger, Greg R.
Riggins, Chance W.
Tranel, Patrick J.
Bernards, Mark L.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CROSS-RESISTANCE
DOSE - RESPONSE
HERBICIDE RESISTANCE
INJURY
topic CROSS-RESISTANCE
DOSE - RESPONSE
HERBICIDE RESISTANCE
INJURY
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv A 2,4-D-resistant tall waterhemp population (FS) from Nebraska was evaluated for resistance to other TIR1 auxin receptor herbicides and to herbicides having alternative mechanisms of action using greenhouse bioassays and genetic markers. Atrazine, imazethapyr, lactofen, mesotrione, glufosinate, and glyphosate were applied in a single-dose bioassay, and tissue was collected from marked plants for genetic analysis. The FS population was not injured by atrazine or by imazethapyr. Approximately 50% of the plants survived lactofen and were actively growing 28 d after treatment. The population was susceptible to mesotrione, glufosinate, and glyphosate. Ametryn, chlorimuron-ethyl, 2,4-D, aminocyclopyraclor, aminopyralid, and picloram were applied in dose - response studies. The FS population was sensitive to ametryn, and the Ser-264-Gly substitution in the D1 protein was not detected, suggesting the lack of response to atrazine is not due to a target-site mutation. The FS population exhibited less than 50% injury to chlorimuron-ethyl at application rates 20 times the labeled use rate. The Ser-653-Asn acetolactate synthase (ALS) substitution, which confers resistance to imidazolinone herbicides, was present in the FS population. However, this does not explain the lack of response to the sulfonylurea herbicide, chlorimuron-ethyl. Sequencing of a portion of the PPX2L gene did not show the ΔG210 mutation that confers resistance to protoporphyrinogen oxidase - inhibiting herbicides, suggesting that other factors were responsible for waterhemp survival after lactofen application. The FS population was confirmed to be at least 30-fold resistant to 2,4-D relative to the susceptible populations. In addition, it was at least 3-fold less sensitive to aminopyralid and picloram, two other TIR1 auxin receptor herbicides, than the 2,4-D-susceptible populations were. These data indicated that the FS population contains both target and non - target site mechanisms conferring resistance to herbicides spanning at least three mechanisms of action: TIR1 auxin receptors, ALS inhibitors, and photosystem II inhibitors.
Fil: Crespo, Roberto Javier. University of Nebraska; Estados Unidos
Fil: Wingeyer, Ana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Paraná; Argentina
Fil: Kruger, Greg R.. University of Nebraska; Estados Unidos
Fil: Riggins, Chance W.. University of Illinois at Urbana; Estados Unidos
Fil: Tranel, Patrick J.. University of Illinois at Urbana; Estados Unidos
Fil: Bernards, Mark L.. University of Nebraska; Estados Unidos
description A 2,4-D-resistant tall waterhemp population (FS) from Nebraska was evaluated for resistance to other TIR1 auxin receptor herbicides and to herbicides having alternative mechanisms of action using greenhouse bioassays and genetic markers. Atrazine, imazethapyr, lactofen, mesotrione, glufosinate, and glyphosate were applied in a single-dose bioassay, and tissue was collected from marked plants for genetic analysis. The FS population was not injured by atrazine or by imazethapyr. Approximately 50% of the plants survived lactofen and were actively growing 28 d after treatment. The population was susceptible to mesotrione, glufosinate, and glyphosate. Ametryn, chlorimuron-ethyl, 2,4-D, aminocyclopyraclor, aminopyralid, and picloram were applied in dose - response studies. The FS population was sensitive to ametryn, and the Ser-264-Gly substitution in the D1 protein was not detected, suggesting the lack of response to atrazine is not due to a target-site mutation. The FS population exhibited less than 50% injury to chlorimuron-ethyl at application rates 20 times the labeled use rate. The Ser-653-Asn acetolactate synthase (ALS) substitution, which confers resistance to imidazolinone herbicides, was present in the FS population. However, this does not explain the lack of response to the sulfonylurea herbicide, chlorimuron-ethyl. Sequencing of a portion of the PPX2L gene did not show the ΔG210 mutation that confers resistance to protoporphyrinogen oxidase - inhibiting herbicides, suggesting that other factors were responsible for waterhemp survival after lactofen application. The FS population was confirmed to be at least 30-fold resistant to 2,4-D relative to the susceptible populations. In addition, it was at least 3-fold less sensitive to aminopyralid and picloram, two other TIR1 auxin receptor herbicides, than the 2,4-D-susceptible populations were. These data indicated that the FS population contains both target and non - target site mechanisms conferring resistance to herbicides spanning at least three mechanisms of action: TIR1 auxin receptors, ALS inhibitors, and photosystem II inhibitors.
publishDate 2017
dc.date.none.fl_str_mv 2017-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/75217
Crespo, Roberto Javier; Wingeyer, Ana Beatriz; Kruger, Greg R.; Riggins, Chance W.; Tranel, Patrick J.; et al.; Multiple-Herbicide Resistance in a 2,4-D - Resistant Waterhemp (Amaranthus tuberculatus) Population from Nebraska; Weed Science Society of America; Weed Science; 65; 6; 11-2017; 743-754
0043-1745
CONICET Digital
CONICET
url http://hdl.handle.net/11336/75217
identifier_str_mv Crespo, Roberto Javier; Wingeyer, Ana Beatriz; Kruger, Greg R.; Riggins, Chance W.; Tranel, Patrick J.; et al.; Multiple-Herbicide Resistance in a 2,4-D - Resistant Waterhemp (Amaranthus tuberculatus) Population from Nebraska; Weed Science Society of America; Weed Science; 65; 6; 11-2017; 743-754
0043-1745
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/weed-science/article/multipleherbicide-resistance-in-a-24dresistant-waterhemp-amaranthus-tuberculatus-population-from-nebraska/9309AB3614CF11C833C658CE65CA1114
info:eu-repo/semantics/altIdentifier/doi/10.1017/wsc.2017.39
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Weed Science Society of America
publisher.none.fl_str_mv Weed Science Society of America
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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