Multiple-Herbicide Resistance in a 2,4-D - Resistant Waterhemp (Amaranthus tuberculatus) Population from Nebraska
- Autores
- Crespo, Roberto Javier; Wingeyer, Ana Beatriz; Kruger, Greg R.; Riggins, Chance W.; Tranel, Patrick J.; Bernards, Mark L.
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- A 2,4-D-resistant tall waterhemp population (FS) from Nebraska was evaluated for resistance to other TIR1 auxin receptor herbicides and to herbicides having alternative mechanisms of action using greenhouse bioassays and genetic markers. Atrazine, imazethapyr, lactofen, mesotrione, glufosinate, and glyphosate were applied in a single-dose bioassay, and tissue was collected from marked plants for genetic analysis. The FS population was not injured by atrazine or by imazethapyr. Approximately 50% of the plants survived lactofen and were actively growing 28 d after treatment. The population was susceptible to mesotrione, glufosinate, and glyphosate. Ametryn, chlorimuron-ethyl, 2,4-D, aminocyclopyraclor, aminopyralid, and picloram were applied in dose - response studies. The FS population was sensitive to ametryn, and the Ser-264-Gly substitution in the D1 protein was not detected, suggesting the lack of response to atrazine is not due to a target-site mutation. The FS population exhibited less than 50% injury to chlorimuron-ethyl at application rates 20 times the labeled use rate. The Ser-653-Asn acetolactate synthase (ALS) substitution, which confers resistance to imidazolinone herbicides, was present in the FS population. However, this does not explain the lack of response to the sulfonylurea herbicide, chlorimuron-ethyl. Sequencing of a portion of the PPX2L gene did not show the ΔG210 mutation that confers resistance to protoporphyrinogen oxidase - inhibiting herbicides, suggesting that other factors were responsible for waterhemp survival after lactofen application. The FS population was confirmed to be at least 30-fold resistant to 2,4-D relative to the susceptible populations. In addition, it was at least 3-fold less sensitive to aminopyralid and picloram, two other TIR1 auxin receptor herbicides, than the 2,4-D-susceptible populations were. These data indicated that the FS population contains both target and non - target site mechanisms conferring resistance to herbicides spanning at least three mechanisms of action: TIR1 auxin receptors, ALS inhibitors, and photosystem II inhibitors.
Fil: Crespo, Roberto Javier. University of Nebraska; Estados Unidos
Fil: Wingeyer, Ana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Paraná; Argentina
Fil: Kruger, Greg R.. University of Nebraska; Estados Unidos
Fil: Riggins, Chance W.. University of Illinois at Urbana; Estados Unidos
Fil: Tranel, Patrick J.. University of Illinois at Urbana; Estados Unidos
Fil: Bernards, Mark L.. University of Nebraska; Estados Unidos - Materia
-
CROSS-RESISTANCE
DOSE - RESPONSE
HERBICIDE RESISTANCE
INJURY - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/75217
Ver los metadatos del registro completo
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Multiple-Herbicide Resistance in a 2,4-D - Resistant Waterhemp (Amaranthus tuberculatus) Population from NebraskaCrespo, Roberto JavierWingeyer, Ana BeatrizKruger, Greg R.Riggins, Chance W.Tranel, Patrick J.Bernards, Mark L.CROSS-RESISTANCEDOSE - RESPONSEHERBICIDE RESISTANCEINJURYhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4A 2,4-D-resistant tall waterhemp population (FS) from Nebraska was evaluated for resistance to other TIR1 auxin receptor herbicides and to herbicides having alternative mechanisms of action using greenhouse bioassays and genetic markers. Atrazine, imazethapyr, lactofen, mesotrione, glufosinate, and glyphosate were applied in a single-dose bioassay, and tissue was collected from marked plants for genetic analysis. The FS population was not injured by atrazine or by imazethapyr. Approximately 50% of the plants survived lactofen and were actively growing 28 d after treatment. The population was susceptible to mesotrione, glufosinate, and glyphosate. Ametryn, chlorimuron-ethyl, 2,4-D, aminocyclopyraclor, aminopyralid, and picloram were applied in dose - response studies. The FS population was sensitive to ametryn, and the Ser-264-Gly substitution in the D1 protein was not detected, suggesting the lack of response to atrazine is not due to a target-site mutation. The FS population exhibited less than 50% injury to chlorimuron-ethyl at application rates 20 times the labeled use rate. The Ser-653-Asn acetolactate synthase (ALS) substitution, which confers resistance to imidazolinone herbicides, was present in the FS population. However, this does not explain the lack of response to the sulfonylurea herbicide, chlorimuron-ethyl. Sequencing of a portion of the PPX2L gene did not show the ΔG210 mutation that confers resistance to protoporphyrinogen oxidase - inhibiting herbicides, suggesting that other factors were responsible for waterhemp survival after lactofen application. The FS population was confirmed to be at least 30-fold resistant to 2,4-D relative to the susceptible populations. In addition, it was at least 3-fold less sensitive to aminopyralid and picloram, two other TIR1 auxin receptor herbicides, than the 2,4-D-susceptible populations were. These data indicated that the FS population contains both target and non - target site mechanisms conferring resistance to herbicides spanning at least three mechanisms of action: TIR1 auxin receptors, ALS inhibitors, and photosystem II inhibitors.Fil: Crespo, Roberto Javier. University of Nebraska; Estados UnidosFil: Wingeyer, Ana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Paraná; ArgentinaFil: Kruger, Greg R.. University of Nebraska; Estados UnidosFil: Riggins, Chance W.. University of Illinois at Urbana; Estados UnidosFil: Tranel, Patrick J.. University of Illinois at Urbana; Estados UnidosFil: Bernards, Mark L.. University of Nebraska; Estados UnidosWeed Science Society of America2017-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/75217Crespo, Roberto Javier; Wingeyer, Ana Beatriz; Kruger, Greg R.; Riggins, Chance W.; Tranel, Patrick J.; et al.; Multiple-Herbicide Resistance in a 2,4-D - Resistant Waterhemp (Amaranthus tuberculatus) Population from Nebraska; Weed Science Society of America; Weed Science; 65; 6; 11-2017; 743-7540043-1745CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/weed-science/article/multipleherbicide-resistance-in-a-24dresistant-waterhemp-amaranthus-tuberculatus-population-from-nebraska/9309AB3614CF11C833C658CE65CA1114info:eu-repo/semantics/altIdentifier/doi/10.1017/wsc.2017.39info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:26:23Zoai:ri.conicet.gov.ar:11336/75217instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:26:24.176CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Multiple-Herbicide Resistance in a 2,4-D - Resistant Waterhemp (Amaranthus tuberculatus) Population from Nebraska |
title |
Multiple-Herbicide Resistance in a 2,4-D - Resistant Waterhemp (Amaranthus tuberculatus) Population from Nebraska |
spellingShingle |
Multiple-Herbicide Resistance in a 2,4-D - Resistant Waterhemp (Amaranthus tuberculatus) Population from Nebraska Crespo, Roberto Javier CROSS-RESISTANCE DOSE - RESPONSE HERBICIDE RESISTANCE INJURY |
title_short |
Multiple-Herbicide Resistance in a 2,4-D - Resistant Waterhemp (Amaranthus tuberculatus) Population from Nebraska |
title_full |
Multiple-Herbicide Resistance in a 2,4-D - Resistant Waterhemp (Amaranthus tuberculatus) Population from Nebraska |
title_fullStr |
Multiple-Herbicide Resistance in a 2,4-D - Resistant Waterhemp (Amaranthus tuberculatus) Population from Nebraska |
title_full_unstemmed |
Multiple-Herbicide Resistance in a 2,4-D - Resistant Waterhemp (Amaranthus tuberculatus) Population from Nebraska |
title_sort |
Multiple-Herbicide Resistance in a 2,4-D - Resistant Waterhemp (Amaranthus tuberculatus) Population from Nebraska |
dc.creator.none.fl_str_mv |
Crespo, Roberto Javier Wingeyer, Ana Beatriz Kruger, Greg R. Riggins, Chance W. Tranel, Patrick J. Bernards, Mark L. |
author |
Crespo, Roberto Javier |
author_facet |
Crespo, Roberto Javier Wingeyer, Ana Beatriz Kruger, Greg R. Riggins, Chance W. Tranel, Patrick J. Bernards, Mark L. |
author_role |
author |
author2 |
Wingeyer, Ana Beatriz Kruger, Greg R. Riggins, Chance W. Tranel, Patrick J. Bernards, Mark L. |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
CROSS-RESISTANCE DOSE - RESPONSE HERBICIDE RESISTANCE INJURY |
topic |
CROSS-RESISTANCE DOSE - RESPONSE HERBICIDE RESISTANCE INJURY |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.4 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
A 2,4-D-resistant tall waterhemp population (FS) from Nebraska was evaluated for resistance to other TIR1 auxin receptor herbicides and to herbicides having alternative mechanisms of action using greenhouse bioassays and genetic markers. Atrazine, imazethapyr, lactofen, mesotrione, glufosinate, and glyphosate were applied in a single-dose bioassay, and tissue was collected from marked plants for genetic analysis. The FS population was not injured by atrazine or by imazethapyr. Approximately 50% of the plants survived lactofen and were actively growing 28 d after treatment. The population was susceptible to mesotrione, glufosinate, and glyphosate. Ametryn, chlorimuron-ethyl, 2,4-D, aminocyclopyraclor, aminopyralid, and picloram were applied in dose - response studies. The FS population was sensitive to ametryn, and the Ser-264-Gly substitution in the D1 protein was not detected, suggesting the lack of response to atrazine is not due to a target-site mutation. The FS population exhibited less than 50% injury to chlorimuron-ethyl at application rates 20 times the labeled use rate. The Ser-653-Asn acetolactate synthase (ALS) substitution, which confers resistance to imidazolinone herbicides, was present in the FS population. However, this does not explain the lack of response to the sulfonylurea herbicide, chlorimuron-ethyl. Sequencing of a portion of the PPX2L gene did not show the ΔG210 mutation that confers resistance to protoporphyrinogen oxidase - inhibiting herbicides, suggesting that other factors were responsible for waterhemp survival after lactofen application. The FS population was confirmed to be at least 30-fold resistant to 2,4-D relative to the susceptible populations. In addition, it was at least 3-fold less sensitive to aminopyralid and picloram, two other TIR1 auxin receptor herbicides, than the 2,4-D-susceptible populations were. These data indicated that the FS population contains both target and non - target site mechanisms conferring resistance to herbicides spanning at least three mechanisms of action: TIR1 auxin receptors, ALS inhibitors, and photosystem II inhibitors. Fil: Crespo, Roberto Javier. University of Nebraska; Estados Unidos Fil: Wingeyer, Ana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Paraná; Argentina Fil: Kruger, Greg R.. University of Nebraska; Estados Unidos Fil: Riggins, Chance W.. University of Illinois at Urbana; Estados Unidos Fil: Tranel, Patrick J.. University of Illinois at Urbana; Estados Unidos Fil: Bernards, Mark L.. University of Nebraska; Estados Unidos |
description |
A 2,4-D-resistant tall waterhemp population (FS) from Nebraska was evaluated for resistance to other TIR1 auxin receptor herbicides and to herbicides having alternative mechanisms of action using greenhouse bioassays and genetic markers. Atrazine, imazethapyr, lactofen, mesotrione, glufosinate, and glyphosate were applied in a single-dose bioassay, and tissue was collected from marked plants for genetic analysis. The FS population was not injured by atrazine or by imazethapyr. Approximately 50% of the plants survived lactofen and were actively growing 28 d after treatment. The population was susceptible to mesotrione, glufosinate, and glyphosate. Ametryn, chlorimuron-ethyl, 2,4-D, aminocyclopyraclor, aminopyralid, and picloram were applied in dose - response studies. The FS population was sensitive to ametryn, and the Ser-264-Gly substitution in the D1 protein was not detected, suggesting the lack of response to atrazine is not due to a target-site mutation. The FS population exhibited less than 50% injury to chlorimuron-ethyl at application rates 20 times the labeled use rate. The Ser-653-Asn acetolactate synthase (ALS) substitution, which confers resistance to imidazolinone herbicides, was present in the FS population. However, this does not explain the lack of response to the sulfonylurea herbicide, chlorimuron-ethyl. Sequencing of a portion of the PPX2L gene did not show the ΔG210 mutation that confers resistance to protoporphyrinogen oxidase - inhibiting herbicides, suggesting that other factors were responsible for waterhemp survival after lactofen application. The FS population was confirmed to be at least 30-fold resistant to 2,4-D relative to the susceptible populations. In addition, it was at least 3-fold less sensitive to aminopyralid and picloram, two other TIR1 auxin receptor herbicides, than the 2,4-D-susceptible populations were. These data indicated that the FS population contains both target and non - target site mechanisms conferring resistance to herbicides spanning at least three mechanisms of action: TIR1 auxin receptors, ALS inhibitors, and photosystem II inhibitors. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/75217 Crespo, Roberto Javier; Wingeyer, Ana Beatriz; Kruger, Greg R.; Riggins, Chance W.; Tranel, Patrick J.; et al.; Multiple-Herbicide Resistance in a 2,4-D - Resistant Waterhemp (Amaranthus tuberculatus) Population from Nebraska; Weed Science Society of America; Weed Science; 65; 6; 11-2017; 743-754 0043-1745 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/75217 |
identifier_str_mv |
Crespo, Roberto Javier; Wingeyer, Ana Beatriz; Kruger, Greg R.; Riggins, Chance W.; Tranel, Patrick J.; et al.; Multiple-Herbicide Resistance in a 2,4-D - Resistant Waterhemp (Amaranthus tuberculatus) Population from Nebraska; Weed Science Society of America; Weed Science; 65; 6; 11-2017; 743-754 0043-1745 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/weed-science/article/multipleherbicide-resistance-in-a-24dresistant-waterhemp-amaranthus-tuberculatus-population-from-nebraska/9309AB3614CF11C833C658CE65CA1114 info:eu-repo/semantics/altIdentifier/doi/10.1017/wsc.2017.39 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Weed Science Society of America |
publisher.none.fl_str_mv |
Weed Science Society of America |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614265479102464 |
score |
13.070432 |