Dna organization along pachytene chromosome axes and its relationship with crossover frequencies

Autores
del Priore, Lucía; Pigozzi, Maria Ines
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
During meiosis, the number of crossovers vary in correlation to the length of prophase chromosome axes at the synaptonemal complex stage. It has been proposed that the regular spacing of the DNA loops, along with the close relationship of the recombination complexes and the meiotic axes are at the basis of this covariation. Here, we use a cytogenomic approach to investigate the relationship between the synaptonemal complex length and the DNA content in chicken oocytes during the pachytene stage of the first meiotic prophase. The synaptonemal complex to DNA ratios of specific chromosomes and chromosome segments were compared against the recombination rates obtained by MLH1 focus mapping. The present results show variations in the DNA packing ratios of macro-and microbivalents and also between regions within the same bivalent. Chromosome or chromosome regions with higher crossover rates form comparatively longer synaptonemal complexes than expected based on their DNA content. These observations are compatible with the formation of higher number of shorter DNA loops along meiotic axes in regions with higher recombination levels.
Fil: del Priore, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Pigozzi, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Materia
BIRD CHROMOSOMES
CROSSING OVER
FLUORESCENT IN SITU HYBRIDIZATION
IMMUNOSTAINING
MEIOSIS
MLH1 FOCUS MAP
MOLECULAR CYTOGENETICS
RECOMBINATION FREQUENCIES
SYNAPTONEMAL COMPLEX
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/170261
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/170261
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Dna organization along pachytene chromosome axes and its relationship with crossover frequenciesdel Priore, LucíaPigozzi, Maria InesBIRD CHROMOSOMESCROSSING OVERFLUORESCENT IN SITU HYBRIDIZATIONIMMUNOSTAININGMEIOSISMLH1 FOCUS MAPMOLECULAR CYTOGENETICSRECOMBINATION FREQUENCIESSYNAPTONEMAL COMPLEXhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1During meiosis, the number of crossovers vary in correlation to the length of prophase chromosome axes at the synaptonemal complex stage. It has been proposed that the regular spacing of the DNA loops, along with the close relationship of the recombination complexes and the meiotic axes are at the basis of this covariation. Here, we use a cytogenomic approach to investigate the relationship between the synaptonemal complex length and the DNA content in chicken oocytes during the pachytene stage of the first meiotic prophase. The synaptonemal complex to DNA ratios of specific chromosomes and chromosome segments were compared against the recombination rates obtained by MLH1 focus mapping. The present results show variations in the DNA packing ratios of macro-and microbivalents and also between regions within the same bivalent. Chromosome or chromosome regions with higher crossover rates form comparatively longer synaptonemal complexes than expected based on their DNA content. These observations are compatible with the formation of higher number of shorter DNA loops along meiotic axes in regions with higher recombination levels.Fil: del Priore, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaFil: Pigozzi, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaMDPI AG2021-02-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/170261del Priore, Lucía; Pigozzi, Maria Ines; Dna organization along pachytene chromosome axes and its relationship with crossover frequencies; MDPI AG; International Journal of Molecular Sciences; 22; 5; 27-2-2021; 1-121422-0067CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/22/5/2414info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms22052414info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:18:11Zoai:ri.conicet.gov.ar:11336/170261instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:18:12.106CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dna organization along pachytene chromosome axes and its relationship with crossover frequencies
title Dna organization along pachytene chromosome axes and its relationship with crossover frequencies
spellingShingle Dna organization along pachytene chromosome axes and its relationship with crossover frequencies
del Priore, Lucía
BIRD CHROMOSOMES
CROSSING OVER
FLUORESCENT IN SITU HYBRIDIZATION
IMMUNOSTAINING
MEIOSIS
MLH1 FOCUS MAP
MOLECULAR CYTOGENETICS
RECOMBINATION FREQUENCIES
SYNAPTONEMAL COMPLEX
title_short Dna organization along pachytene chromosome axes and its relationship with crossover frequencies
title_full Dna organization along pachytene chromosome axes and its relationship with crossover frequencies
title_fullStr Dna organization along pachytene chromosome axes and its relationship with crossover frequencies
title_full_unstemmed Dna organization along pachytene chromosome axes and its relationship with crossover frequencies
title_sort Dna organization along pachytene chromosome axes and its relationship with crossover frequencies
dc.creator.none.fl_str_mv del Priore, Lucía
Pigozzi, Maria Ines
author del Priore, Lucía
author_facet del Priore, Lucía
Pigozzi, Maria Ines
author_role author
author2 Pigozzi, Maria Ines
author2_role author
dc.subject.none.fl_str_mv BIRD CHROMOSOMES
CROSSING OVER
FLUORESCENT IN SITU HYBRIDIZATION
IMMUNOSTAINING
MEIOSIS
MLH1 FOCUS MAP
MOLECULAR CYTOGENETICS
RECOMBINATION FREQUENCIES
SYNAPTONEMAL COMPLEX
topic BIRD CHROMOSOMES
CROSSING OVER
FLUORESCENT IN SITU HYBRIDIZATION
IMMUNOSTAINING
MEIOSIS
MLH1 FOCUS MAP
MOLECULAR CYTOGENETICS
RECOMBINATION FREQUENCIES
SYNAPTONEMAL COMPLEX
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv During meiosis, the number of crossovers vary in correlation to the length of prophase chromosome axes at the synaptonemal complex stage. It has been proposed that the regular spacing of the DNA loops, along with the close relationship of the recombination complexes and the meiotic axes are at the basis of this covariation. Here, we use a cytogenomic approach to investigate the relationship between the synaptonemal complex length and the DNA content in chicken oocytes during the pachytene stage of the first meiotic prophase. The synaptonemal complex to DNA ratios of specific chromosomes and chromosome segments were compared against the recombination rates obtained by MLH1 focus mapping. The present results show variations in the DNA packing ratios of macro-and microbivalents and also between regions within the same bivalent. Chromosome or chromosome regions with higher crossover rates form comparatively longer synaptonemal complexes than expected based on their DNA content. These observations are compatible with the formation of higher number of shorter DNA loops along meiotic axes in regions with higher recombination levels.
Fil: del Priore, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Pigozzi, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
description During meiosis, the number of crossovers vary in correlation to the length of prophase chromosome axes at the synaptonemal complex stage. It has been proposed that the regular spacing of the DNA loops, along with the close relationship of the recombination complexes and the meiotic axes are at the basis of this covariation. Here, we use a cytogenomic approach to investigate the relationship between the synaptonemal complex length and the DNA content in chicken oocytes during the pachytene stage of the first meiotic prophase. The synaptonemal complex to DNA ratios of specific chromosomes and chromosome segments were compared against the recombination rates obtained by MLH1 focus mapping. The present results show variations in the DNA packing ratios of macro-and microbivalents and also between regions within the same bivalent. Chromosome or chromosome regions with higher crossover rates form comparatively longer synaptonemal complexes than expected based on their DNA content. These observations are compatible with the formation of higher number of shorter DNA loops along meiotic axes in regions with higher recombination levels.
publishDate 2021
dc.date.none.fl_str_mv 2021-02-27
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/170261
del Priore, Lucía; Pigozzi, Maria Ines; Dna organization along pachytene chromosome axes and its relationship with crossover frequencies; MDPI AG; International Journal of Molecular Sciences; 22; 5; 27-2-2021; 1-12
1422-0067
CONICET Digital
CONICET
url http://hdl.handle.net/11336/170261
identifier_str_mv del Priore, Lucía; Pigozzi, Maria Ines; Dna organization along pachytene chromosome axes and its relationship with crossover frequencies; MDPI AG; International Journal of Molecular Sciences; 22; 5; 27-2-2021; 1-12
1422-0067
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/22/5/2414
info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms22052414
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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