Sex-specific recombination maps for individual macrochromosomes in the Japanese quail (Coturnix japonica)

Autores
del Priore, Lucía; Pigozzi, Maria Ines
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Meiotic recombination in the Japanese quail was directly studied by immunolocalization of mutL homolog 1 (MLH1), a mismatch repair protein of mature recombination nodules. In total, 15,862 crossovers were scored along the autosomal synaptonemal complexes in 308 meiotic nuclei from males and females. Crossover frequencies calculated from MLH1 foci show wide similitude between males and females with slightly higher number of foci in females. From this analysis, we predict that the sex-averaged map length of the Japanese quail is 2580 cM, with a genome-wide recombination rate of 1.9 cM/Mb. MLH1 focus mapping along the six largest bivalents showed few intersex differences in the distribution of crossovers along with variant patterns in metacentric and acrocentric macrobivalents. These results provide valuable information to complement linkage map analysis in the species while providing insight into our understanding of the mechanisms of crossover distribution along chromosome arms.
Fil: del Priore, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Pigozzi, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Materia
Birds
Meiosis
Crossing Over
Mlh1 Foci
Poultry Genome
Japanase Quail
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/15276
Acceder
id CONICETDig_536baa6109b4edf29248755ba14b0a5f
oai_identifier_str oai:ri.conicet.gov.ar:11336/15276
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sex-specific recombination maps for individual macrochromosomes in the Japanese quail (Coturnix japonica)del Priore, LucíaPigozzi, Maria InesBirdsMeiosisCrossing OverMlh1 FociPoultry GenomeJapanase Quailhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Meiotic recombination in the Japanese quail was directly studied by immunolocalization of mutL homolog 1 (MLH1), a mismatch repair protein of mature recombination nodules. In total, 15,862 crossovers were scored along the autosomal synaptonemal complexes in 308 meiotic nuclei from males and females. Crossover frequencies calculated from MLH1 foci show wide similitude between males and females with slightly higher number of foci in females. From this analysis, we predict that the sex-averaged map length of the Japanese quail is 2580 cM, with a genome-wide recombination rate of 1.9 cM/Mb. MLH1 focus mapping along the six largest bivalents showed few intersex differences in the distribution of crossovers along with variant patterns in metacentric and acrocentric macrobivalents. These results provide valuable information to complement linkage map analysis in the species while providing insight into our understanding of the mechanisms of crossover distribution along chromosome arms.Fil: del Priore, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Pigozzi, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaSpringer2015-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/15276del Priore, Lucía; Pigozzi, Maria Ines; Sex-specific recombination maps for individual macrochromosomes in the Japanese quail (Coturnix japonica); Springer; Chromosome Research; 23; 2; 6-2015; 199-2100967-38491573-6849enginfo:eu-repo/semantics/altIdentifier/url/http://www.springer.com/life+sciences/cell+biology/journal/10577info:eu-repo/semantics/altIdentifier/doi/10.1007/s10577-014-9448-2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:14Zoai:ri.conicet.gov.ar:11336/15276instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:14.591CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sex-specific recombination maps for individual macrochromosomes in the Japanese quail (Coturnix japonica)
title Sex-specific recombination maps for individual macrochromosomes in the Japanese quail (Coturnix japonica)
spellingShingle Sex-specific recombination maps for individual macrochromosomes in the Japanese quail (Coturnix japonica)
del Priore, Lucía
Birds
Meiosis
Crossing Over
Mlh1 Foci
Poultry Genome
Japanase Quail
title_short Sex-specific recombination maps for individual macrochromosomes in the Japanese quail (Coturnix japonica)
title_full Sex-specific recombination maps for individual macrochromosomes in the Japanese quail (Coturnix japonica)
title_fullStr Sex-specific recombination maps for individual macrochromosomes in the Japanese quail (Coturnix japonica)
title_full_unstemmed Sex-specific recombination maps for individual macrochromosomes in the Japanese quail (Coturnix japonica)
title_sort Sex-specific recombination maps for individual macrochromosomes in the Japanese quail (Coturnix japonica)
dc.creator.none.fl_str_mv del Priore, Lucía
Pigozzi, Maria Ines
author del Priore, Lucía
author_facet del Priore, Lucía
Pigozzi, Maria Ines
author_role author
author2 Pigozzi, Maria Ines
author2_role author
dc.subject.none.fl_str_mv Birds
Meiosis
Crossing Over
Mlh1 Foci
Poultry Genome
Japanase Quail
topic Birds
Meiosis
Crossing Over
Mlh1 Foci
Poultry Genome
Japanase Quail
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Meiotic recombination in the Japanese quail was directly studied by immunolocalization of mutL homolog 1 (MLH1), a mismatch repair protein of mature recombination nodules. In total, 15,862 crossovers were scored along the autosomal synaptonemal complexes in 308 meiotic nuclei from males and females. Crossover frequencies calculated from MLH1 foci show wide similitude between males and females with slightly higher number of foci in females. From this analysis, we predict that the sex-averaged map length of the Japanese quail is 2580 cM, with a genome-wide recombination rate of 1.9 cM/Mb. MLH1 focus mapping along the six largest bivalents showed few intersex differences in the distribution of crossovers along with variant patterns in metacentric and acrocentric macrobivalents. These results provide valuable information to complement linkage map analysis in the species while providing insight into our understanding of the mechanisms of crossover distribution along chromosome arms.
Fil: del Priore, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Pigozzi, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
description Meiotic recombination in the Japanese quail was directly studied by immunolocalization of mutL homolog 1 (MLH1), a mismatch repair protein of mature recombination nodules. In total, 15,862 crossovers were scored along the autosomal synaptonemal complexes in 308 meiotic nuclei from males and females. Crossover frequencies calculated from MLH1 foci show wide similitude between males and females with slightly higher number of foci in females. From this analysis, we predict that the sex-averaged map length of the Japanese quail is 2580 cM, with a genome-wide recombination rate of 1.9 cM/Mb. MLH1 focus mapping along the six largest bivalents showed few intersex differences in the distribution of crossovers along with variant patterns in metacentric and acrocentric macrobivalents. These results provide valuable information to complement linkage map analysis in the species while providing insight into our understanding of the mechanisms of crossover distribution along chromosome arms.
publishDate 2015
dc.date.none.fl_str_mv 2015-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/15276
del Priore, Lucía; Pigozzi, Maria Ines; Sex-specific recombination maps for individual macrochromosomes in the Japanese quail (Coturnix japonica); Springer; Chromosome Research; 23; 2; 6-2015; 199-210
0967-3849
1573-6849
url http://hdl.handle.net/11336/15276
identifier_str_mv del Priore, Lucía; Pigozzi, Maria Ines; Sex-specific recombination maps for individual macrochromosomes in the Japanese quail (Coturnix japonica); Springer; Chromosome Research; 23; 2; 6-2015; 199-210
0967-3849
1573-6849
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.springer.com/life+sciences/cell+biology/journal/10577
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10577-014-9448-2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613444735598592
score 13.070432

Publicaciones similares