Assessment of biogenic silica potential for biomedical applications

Autores
Navarro, Francisco Ezequiel; Busso, Marina Belen; Agotegaray, Mariela Alejandra; Horst, María Fernanda; Damiani, Maria Cecilia; Sanchez, Miguel Dario; Popovich, Cecilia Angelines
Año de publicación
2024
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Diatoms are eukaryotic microalgae, which present a cell wall made of amorphous silica called frustule. Studies in micro/nano-technology show that frustules are potential materials for various applications, such as drug delivery, biosensors, and rapid bleeding control. The aim of this study was to evaluate the physicochemical properties and blood tissue biocompatibility of frustules extracted from Halamphora coffeaeformis, a diatom native from Bahía Blanca Estuary, to assess their potential application in biomedical fields. Frustules were obtained from two different culture methods: photobioreactor (F-PBR) and open raceway pond (F-RAC). Both cultures underwent an oxidation treatment with hydrogen peroxide at 80ºC for 12h. Afterwards, the frustules were subjected to three calcination treatments at 300ºC, 500ºC, and 800ºC, based on mass loss and stabilization identified through thermogravimetric analysis. Significant changes were observed in the composition and surface properties of the frustules after the treatments. In general, the percentages of Si and O tended to increase as temperatures rose, while C content decreased. This trend corresponded with a reduction in C=O and C-H groups at higher temperatures. BET surface area values were highest at 300ºC for both cultivation methods, with F-RAC (68.6 m²/g) significantly surpassing F-PBR (27 m²/g). Additionally, z-potential measurements indicated higher stability for F-PBR (-33 mV) compared to F-RAC (-20 mV), suggesting better dispersion stability in an aqueous medium and fewer aggregates. The preliminary hemocompatibility study, displayed by evaluation of hemolysis and osmotic fragility on red blood cells, showed biocompatibility of frustules with erythrocytes. In conclusion, H. coffeaeformis frustules, particularly those from photobioreactor cultivation and calcined at 300ºC, possess favorable physicochemical properties and biocompatibility with blood tissue. These characteristics highlight their potential as drug carriers.
Fil: Navarro, Francisco Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Busso, Marina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Agotegaray, Mariela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Horst, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Damiani, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Sanchez, Miguel Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Física del Sur. Universidad Nacional del Sur. Departamento de Física. Instituto de Física del Sur; Argentina
Fil: Popovich, Cecilia Angelines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
LVI Reunión Anual de la AAFE
Bahía Blanca
Argentina
Universidad Nacional del Sur
Materia
DIATOMEAS
NANOTECNOLOGÍA
BIOMEDICINA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/281604
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Assessment of biogenic silica potential for biomedical applicationsNavarro, Francisco EzequielBusso, Marina BelenAgotegaray, Mariela AlejandraHorst, María FernandaDamiani, Maria CeciliaSanchez, Miguel DarioPopovich, Cecilia AngelinesDIATOMEASNANOTECNOLOGÍABIOMEDICINAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Diatoms are eukaryotic microalgae, which present a cell wall made of amorphous silica called frustule. Studies in micro/nano-technology show that frustules are potential materials for various applications, such as drug delivery, biosensors, and rapid bleeding control. The aim of this study was to evaluate the physicochemical properties and blood tissue biocompatibility of frustules extracted from Halamphora coffeaeformis, a diatom native from Bahía Blanca Estuary, to assess their potential application in biomedical fields. Frustules were obtained from two different culture methods: photobioreactor (F-PBR) and open raceway pond (F-RAC). Both cultures underwent an oxidation treatment with hydrogen peroxide at 80ºC for 12h. Afterwards, the frustules were subjected to three calcination treatments at 300ºC, 500ºC, and 800ºC, based on mass loss and stabilization identified through thermogravimetric analysis. Significant changes were observed in the composition and surface properties of the frustules after the treatments. In general, the percentages of Si and O tended to increase as temperatures rose, while C content decreased. This trend corresponded with a reduction in C=O and C-H groups at higher temperatures. BET surface area values were highest at 300ºC for both cultivation methods, with F-RAC (68.6 m²/g) significantly surpassing F-PBR (27 m²/g). Additionally, z-potential measurements indicated higher stability for F-PBR (-33 mV) compared to F-RAC (-20 mV), suggesting better dispersion stability in an aqueous medium and fewer aggregates. The preliminary hemocompatibility study, displayed by evaluation of hemolysis and osmotic fragility on red blood cells, showed biocompatibility of frustules with erythrocytes. In conclusion, H. coffeaeformis frustules, particularly those from photobioreactor cultivation and calcined at 300ºC, possess favorable physicochemical properties and biocompatibility with blood tissue. These characteristics highlight their potential as drug carriers.Fil: Navarro, Francisco Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Busso, Marina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Agotegaray, Mariela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Horst, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Damiani, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Sanchez, Miguel Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Física del Sur. Universidad Nacional del Sur. Departamento de Física. Instituto de Física del Sur; ArgentinaFil: Popovich, Cecilia Angelines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaLVI Reunión Anual de la AAFEBahía BlancaArgentinaUniversidad Nacional del SurAsociación Argentina de Farmacología ExperimentalHernando, Guillermina Silvana2024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/281604Assessment of biogenic silica potential for biomedical applications; LVI Reunión Anual de la AAFE; Bahía Blanca; Argentina; 2024; 66-66978-631-90806-0-5CONICET DigitalCONICETenghttps://aafeargentina.org/congreso-aafe-2024-2/https://civetan.conicet.gov.ar/reunion-anual-aafe-2024/info:eu-repo/semantics/altIdentifier/url/https://aafeargentina.org/congreso-aafe-2024-2/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-03-31T15:14:18Zoai:ri.conicet.gov.ar:11336/281604instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-03-31 15:14:19.069CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Assessment of biogenic silica potential for biomedical applications
title Assessment of biogenic silica potential for biomedical applications
spellingShingle Assessment of biogenic silica potential for biomedical applications
Navarro, Francisco Ezequiel
DIATOMEAS
NANOTECNOLOGÍA
BIOMEDICINA
title_short Assessment of biogenic silica potential for biomedical applications
title_full Assessment of biogenic silica potential for biomedical applications
title_fullStr Assessment of biogenic silica potential for biomedical applications
title_full_unstemmed Assessment of biogenic silica potential for biomedical applications
title_sort Assessment of biogenic silica potential for biomedical applications
dc.creator.none.fl_str_mv Navarro, Francisco Ezequiel
Busso, Marina Belen
Agotegaray, Mariela Alejandra
Horst, María Fernanda
Damiani, Maria Cecilia
Sanchez, Miguel Dario
Popovich, Cecilia Angelines
author Navarro, Francisco Ezequiel
author_facet Navarro, Francisco Ezequiel
Busso, Marina Belen
Agotegaray, Mariela Alejandra
Horst, María Fernanda
Damiani, Maria Cecilia
Sanchez, Miguel Dario
Popovich, Cecilia Angelines
author_role author
author2 Busso, Marina Belen
Agotegaray, Mariela Alejandra
Horst, María Fernanda
Damiani, Maria Cecilia
Sanchez, Miguel Dario
Popovich, Cecilia Angelines
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Hernando, Guillermina Silvana
dc.subject.none.fl_str_mv DIATOMEAS
NANOTECNOLOGÍA
BIOMEDICINA
topic DIATOMEAS
NANOTECNOLOGÍA
BIOMEDICINA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Diatoms are eukaryotic microalgae, which present a cell wall made of amorphous silica called frustule. Studies in micro/nano-technology show that frustules are potential materials for various applications, such as drug delivery, biosensors, and rapid bleeding control. The aim of this study was to evaluate the physicochemical properties and blood tissue biocompatibility of frustules extracted from Halamphora coffeaeformis, a diatom native from Bahía Blanca Estuary, to assess their potential application in biomedical fields. Frustules were obtained from two different culture methods: photobioreactor (F-PBR) and open raceway pond (F-RAC). Both cultures underwent an oxidation treatment with hydrogen peroxide at 80ºC for 12h. Afterwards, the frustules were subjected to three calcination treatments at 300ºC, 500ºC, and 800ºC, based on mass loss and stabilization identified through thermogravimetric analysis. Significant changes were observed in the composition and surface properties of the frustules after the treatments. In general, the percentages of Si and O tended to increase as temperatures rose, while C content decreased. This trend corresponded with a reduction in C=O and C-H groups at higher temperatures. BET surface area values were highest at 300ºC for both cultivation methods, with F-RAC (68.6 m²/g) significantly surpassing F-PBR (27 m²/g). Additionally, z-potential measurements indicated higher stability for F-PBR (-33 mV) compared to F-RAC (-20 mV), suggesting better dispersion stability in an aqueous medium and fewer aggregates. The preliminary hemocompatibility study, displayed by evaluation of hemolysis and osmotic fragility on red blood cells, showed biocompatibility of frustules with erythrocytes. In conclusion, H. coffeaeformis frustules, particularly those from photobioreactor cultivation and calcined at 300ºC, possess favorable physicochemical properties and biocompatibility with blood tissue. These characteristics highlight their potential as drug carriers.
Fil: Navarro, Francisco Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Busso, Marina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Agotegaray, Mariela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Horst, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Damiani, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Sanchez, Miguel Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Física del Sur. Universidad Nacional del Sur. Departamento de Física. Instituto de Física del Sur; Argentina
Fil: Popovich, Cecilia Angelines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
LVI Reunión Anual de la AAFE
Bahía Blanca
Argentina
Universidad Nacional del Sur
description Diatoms are eukaryotic microalgae, which present a cell wall made of amorphous silica called frustule. Studies in micro/nano-technology show that frustules are potential materials for various applications, such as drug delivery, biosensors, and rapid bleeding control. The aim of this study was to evaluate the physicochemical properties and blood tissue biocompatibility of frustules extracted from Halamphora coffeaeformis, a diatom native from Bahía Blanca Estuary, to assess their potential application in biomedical fields. Frustules were obtained from two different culture methods: photobioreactor (F-PBR) and open raceway pond (F-RAC). Both cultures underwent an oxidation treatment with hydrogen peroxide at 80ºC for 12h. Afterwards, the frustules were subjected to three calcination treatments at 300ºC, 500ºC, and 800ºC, based on mass loss and stabilization identified through thermogravimetric analysis. Significant changes were observed in the composition and surface properties of the frustules after the treatments. In general, the percentages of Si and O tended to increase as temperatures rose, while C content decreased. This trend corresponded with a reduction in C=O and C-H groups at higher temperatures. BET surface area values were highest at 300ºC for both cultivation methods, with F-RAC (68.6 m²/g) significantly surpassing F-PBR (27 m²/g). Additionally, z-potential measurements indicated higher stability for F-PBR (-33 mV) compared to F-RAC (-20 mV), suggesting better dispersion stability in an aqueous medium and fewer aggregates. The preliminary hemocompatibility study, displayed by evaluation of hemolysis and osmotic fragility on red blood cells, showed biocompatibility of frustules with erythrocytes. In conclusion, H. coffeaeformis frustules, particularly those from photobioreactor cultivation and calcined at 300ºC, possess favorable physicochemical properties and biocompatibility with blood tissue. These characteristics highlight their potential as drug carriers.
publishDate 2024
dc.date.none.fl_str_mv 2024
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info:eu-repo/semantics/conferenceObject
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Book
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info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/281604
Assessment of biogenic silica potential for biomedical applications; LVI Reunión Anual de la AAFE; Bahía Blanca; Argentina; 2024; 66-66
978-631-90806-0-5
CONICET Digital
CONICET
url http://hdl.handle.net/11336/281604
identifier_str_mv Assessment of biogenic silica potential for biomedical applications; LVI Reunión Anual de la AAFE; Bahía Blanca; Argentina; 2024; 66-66
978-631-90806-0-5
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Asociación Argentina de Farmacología Experimental
publisher.none.fl_str_mv Asociación Argentina de Farmacología Experimental
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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