Interplay Between Electrophoretic Mobility and Intrinsic Viscosity of Polypeptide Chains

Autores
Deiber, Julio Alcides; Peirotti, Marta Beatriz; Piaggio, María Virginia
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The present work is motivated specifically by the need to find a simple interplay between experimental values of electrophoreticmobility and intrinsic viscosity (IV) of polypeptides. The connection between these two properties, as they are evaluated experimentally in a formulated dilute solution,may provide relevant information concerning the physicochemical characterization and separation of electrically charged chains such as polypeptides. Based on this aspect, a study on the relation between the effective electrophoretic mobility and the IV of the following globular proteins is carried out: bovine carbonic anhydrase, staphylococcal nuclease, human carbonic anhydrase, lysozyme, human serum albumin. The basic interpretation of the IV through polypeptide chain conformations involves two unknowns: one is the Flory characteristic ratio involving short-range intramolecular interactions and the other is the Mark–Houwink exponent associated with large-range intramolecular interactions. Here, it will be shown via basic and well-established electrokinetic theories and scaling concepts that the IV and global chain flexibility of polypeptides in dilute solutions may be estimated from capillary zone electrophoresis, in addition to classical transport properties. The polypeptide local chain flexibility may change due to electrostatic interactions among closer chain ionizing groups and the hindrance effect of their associated structural water.
Fil: Deiber, Julio Alcides. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Peirotti, Marta Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Piaggio, María Virginia. Universidad Nacional del Litoral; Argentina
Materia
Electrophoretic Mobility
Friction Power Coefficient
Flory Characteristic Ratio
Global Chain Flexibility
Intrinsic Viscosity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/18770
Acceder
id CONICETDig_056d3881e0c58e09c9dcb07a0823e030
oai_identifier_str oai:ri.conicet.gov.ar:11336/18770
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Interplay Between Electrophoretic Mobility and Intrinsic Viscosity of Polypeptide ChainsDeiber, Julio AlcidesPeirotti, Marta BeatrizPiaggio, María VirginiaElectrophoretic MobilityFriction Power CoefficientFlory Characteristic RatioGlobal Chain FlexibilityIntrinsic Viscosityhttps://purl.org/becyt/ford/2.5https://purl.org/becyt/ford/2The present work is motivated specifically by the need to find a simple interplay between experimental values of electrophoreticmobility and intrinsic viscosity (IV) of polypeptides. The connection between these two properties, as they are evaluated experimentally in a formulated dilute solution,may provide relevant information concerning the physicochemical characterization and separation of electrically charged chains such as polypeptides. Based on this aspect, a study on the relation between the effective electrophoretic mobility and the IV of the following globular proteins is carried out: bovine carbonic anhydrase, staphylococcal nuclease, human carbonic anhydrase, lysozyme, human serum albumin. The basic interpretation of the IV through polypeptide chain conformations involves two unknowns: one is the Flory characteristic ratio involving short-range intramolecular interactions and the other is the Mark–Houwink exponent associated with large-range intramolecular interactions. Here, it will be shown via basic and well-established electrokinetic theories and scaling concepts that the IV and global chain flexibility of polypeptides in dilute solutions may be estimated from capillary zone electrophoresis, in addition to classical transport properties. The polypeptide local chain flexibility may change due to electrostatic interactions among closer chain ionizing groups and the hindrance effect of their associated structural water.Fil: Deiber, Julio Alcides. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Peirotti, Marta Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Piaggio, María Virginia. Universidad Nacional del Litoral; ArgentinaWiley Vch Verlag2012-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/18770Deiber, Julio Alcides; Peirotti, Marta Beatriz; Piaggio, María Virginia; Interplay Between Electrophoretic Mobility and Intrinsic Viscosity of Polypeptide Chains; Wiley Vch Verlag; Electrophoresis; 33; 6; 4-2012; 990-9990173-0835CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/elps.201100637info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/elps.201100637/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:15:27Zoai:ri.conicet.gov.ar:11336/18770instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:15:27.669CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Interplay Between Electrophoretic Mobility and Intrinsic Viscosity of Polypeptide Chains
title Interplay Between Electrophoretic Mobility and Intrinsic Viscosity of Polypeptide Chains
spellingShingle Interplay Between Electrophoretic Mobility and Intrinsic Viscosity of Polypeptide Chains
Deiber, Julio Alcides
Electrophoretic Mobility
Friction Power Coefficient
Flory Characteristic Ratio
Global Chain Flexibility
Intrinsic Viscosity
title_short Interplay Between Electrophoretic Mobility and Intrinsic Viscosity of Polypeptide Chains
title_full Interplay Between Electrophoretic Mobility and Intrinsic Viscosity of Polypeptide Chains
title_fullStr Interplay Between Electrophoretic Mobility and Intrinsic Viscosity of Polypeptide Chains
title_full_unstemmed Interplay Between Electrophoretic Mobility and Intrinsic Viscosity of Polypeptide Chains
title_sort Interplay Between Electrophoretic Mobility and Intrinsic Viscosity of Polypeptide Chains
dc.creator.none.fl_str_mv Deiber, Julio Alcides
Peirotti, Marta Beatriz
Piaggio, María Virginia
author Deiber, Julio Alcides
author_facet Deiber, Julio Alcides
Peirotti, Marta Beatriz
Piaggio, María Virginia
author_role author
author2 Peirotti, Marta Beatriz
Piaggio, María Virginia
author2_role author
author
dc.subject.none.fl_str_mv Electrophoretic Mobility
Friction Power Coefficient
Flory Characteristic Ratio
Global Chain Flexibility
Intrinsic Viscosity
topic Electrophoretic Mobility
Friction Power Coefficient
Flory Characteristic Ratio
Global Chain Flexibility
Intrinsic Viscosity
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.5
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The present work is motivated specifically by the need to find a simple interplay between experimental values of electrophoreticmobility and intrinsic viscosity (IV) of polypeptides. The connection between these two properties, as they are evaluated experimentally in a formulated dilute solution,may provide relevant information concerning the physicochemical characterization and separation of electrically charged chains such as polypeptides. Based on this aspect, a study on the relation between the effective electrophoretic mobility and the IV of the following globular proteins is carried out: bovine carbonic anhydrase, staphylococcal nuclease, human carbonic anhydrase, lysozyme, human serum albumin. The basic interpretation of the IV through polypeptide chain conformations involves two unknowns: one is the Flory characteristic ratio involving short-range intramolecular interactions and the other is the Mark–Houwink exponent associated with large-range intramolecular interactions. Here, it will be shown via basic and well-established electrokinetic theories and scaling concepts that the IV and global chain flexibility of polypeptides in dilute solutions may be estimated from capillary zone electrophoresis, in addition to classical transport properties. The polypeptide local chain flexibility may change due to electrostatic interactions among closer chain ionizing groups and the hindrance effect of their associated structural water.
Fil: Deiber, Julio Alcides. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Peirotti, Marta Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Piaggio, María Virginia. Universidad Nacional del Litoral; Argentina
description The present work is motivated specifically by the need to find a simple interplay between experimental values of electrophoreticmobility and intrinsic viscosity (IV) of polypeptides. The connection between these two properties, as they are evaluated experimentally in a formulated dilute solution,may provide relevant information concerning the physicochemical characterization and separation of electrically charged chains such as polypeptides. Based on this aspect, a study on the relation between the effective electrophoretic mobility and the IV of the following globular proteins is carried out: bovine carbonic anhydrase, staphylococcal nuclease, human carbonic anhydrase, lysozyme, human serum albumin. The basic interpretation of the IV through polypeptide chain conformations involves two unknowns: one is the Flory characteristic ratio involving short-range intramolecular interactions and the other is the Mark–Houwink exponent associated with large-range intramolecular interactions. Here, it will be shown via basic and well-established electrokinetic theories and scaling concepts that the IV and global chain flexibility of polypeptides in dilute solutions may be estimated from capillary zone electrophoresis, in addition to classical transport properties. The polypeptide local chain flexibility may change due to electrostatic interactions among closer chain ionizing groups and the hindrance effect of their associated structural water.
publishDate 2012
dc.date.none.fl_str_mv 2012-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/18770
Deiber, Julio Alcides; Peirotti, Marta Beatriz; Piaggio, María Virginia; Interplay Between Electrophoretic Mobility and Intrinsic Viscosity of Polypeptide Chains; Wiley Vch Verlag; Electrophoresis; 33; 6; 4-2012; 990-999
0173-0835
CONICET Digital
CONICET
url http://hdl.handle.net/11336/18770
identifier_str_mv Deiber, Julio Alcides; Peirotti, Marta Beatriz; Piaggio, María Virginia; Interplay Between Electrophoretic Mobility and Intrinsic Viscosity of Polypeptide Chains; Wiley Vch Verlag; Electrophoresis; 33; 6; 4-2012; 990-999
0173-0835
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/elps.201100637
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/elps.201100637/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Vch Verlag
publisher.none.fl_str_mv Wiley Vch Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083302456295424
score 13.22299

Publicaciones similares