Staining tumor cells with biotinylated ACL-I, a lectin isolated from the marine sponge, Axinella corrugata

Autores
Dresch, R. R.; Zanetti, G. D.; Irazoqui, Fernando Jose; Sendra, Victor German; Zlocowski, Natacha; Bernardi, A.; Rosa, R. M.; Battastini, A. M. O.; Henriques, Amalia Teresinha; Vozári Hampe, M. M.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Axinella corrugata lectin 1 (ACL-1) was purifi ed from aqueous extracts of the marine sponge, Axinella corrugata . ACL-1 strongly agglutinates native rabbit erythrocytes. The hemagglutination is inhibited by N -acetyl derivatives, particularly N , N ’ , N ” -triacetylchitotriose, N -acetyl-Dglucosamine, N -acetyl-D-mannosamine and N -acetyl-D-galactosamine. We investigated the capacity of biotinylated ACL-1 to stain several transformed cell lines including breast (T-47D, MCF7), colon (HT-29), lung (H460), ovary (OVCAR-3) and bladder (T24). ACL-I may bind to both monosaccharides and oligosaccharides of tumor cells, N -acetyl-D-galactosamine, and N -acetyl-Dglucosamine glycan types. The lectins are useful, not only as markers and diagnostic parameters, but also for tissue mapping in suspicious neoplasms. In addition, they provide a better understanding of neoplasms at the cytological and molecular levels. Furthermore, the use of potential metastatic markers such as lectins is crucial for developing successful tools for therapy against cancer. We observed that biotinylated ACL-I stains tumor cells and may hold potential as a probe for identifying transformed cells and for studying glycan structures synthesized by such cells.
Fil: Dresch, R. R.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Zanetti, G. D.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Irazoqui, Fernando Jose. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Quimica Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sendra, Victor German. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Quimica Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zlocowski, Natacha. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Quimica Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bernardi, A.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Rosa, R. M.. Universidade Luterana do Brasil; Brasil
Fil: Battastini, A. M. O.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Henriques, Amalia Teresinha. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Vozári Hampe, M. M.. Universidade Federal do Rio Grande do Sul; Brasil
Materia
Axinella Corrugata
Cancer
Carbohydrate
Lectin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24985
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/24985
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Staining tumor cells with biotinylated ACL-I, a lectin isolated from the marine sponge, Axinella corrugataDresch, R. R.Zanetti, G. D.Irazoqui, Fernando JoseSendra, Victor GermanZlocowski, NatachaBernardi, A.Rosa, R. M.Battastini, A. M. O.Henriques, Amalia TeresinhaVozári Hampe, M. M.Axinella CorrugataCancerCarbohydrateLectinhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Axinella corrugata lectin 1 (ACL-1) was purifi ed from aqueous extracts of the marine sponge, Axinella corrugata . ACL-1 strongly agglutinates native rabbit erythrocytes. The hemagglutination is inhibited by N -acetyl derivatives, particularly N , N ’ , N ” -triacetylchitotriose, N -acetyl-Dglucosamine, N -acetyl-D-mannosamine and N -acetyl-D-galactosamine. We investigated the capacity of biotinylated ACL-1 to stain several transformed cell lines including breast (T-47D, MCF7), colon (HT-29), lung (H460), ovary (OVCAR-3) and bladder (T24). ACL-I may bind to both monosaccharides and oligosaccharides of tumor cells, N -acetyl-D-galactosamine, and N -acetyl-Dglucosamine glycan types. The lectins are useful, not only as markers and diagnostic parameters, but also for tissue mapping in suspicious neoplasms. In addition, they provide a better understanding of neoplasms at the cytological and molecular levels. Furthermore, the use of potential metastatic markers such as lectins is crucial for developing successful tools for therapy against cancer. We observed that biotinylated ACL-I stains tumor cells and may hold potential as a probe for identifying transformed cells and for studying glycan structures synthesized by such cells.Fil: Dresch, R. R.. Universidade Federal do Rio Grande do Sul; BrasilFil: Zanetti, G. D.. Universidade Federal do Rio Grande do Sul; BrasilFil: Irazoqui, Fernando Jose. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Quimica Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sendra, Victor German. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Quimica Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zlocowski, Natacha. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Quimica Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bernardi, A.. Universidade Federal do Rio Grande do Sul; BrasilFil: Rosa, R. M.. Universidade Luterana do Brasil; BrasilFil: Battastini, A. M. O.. Universidade Federal do Rio Grande do Sul; BrasilFil: Henriques, Amalia Teresinha. Universidade Federal do Rio Grande do Sul; BrasilFil: Vozári Hampe, M. M.. Universidade Federal do Rio Grande do Sul; BrasilTaylor & Francis2012-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24985Dresch, R. R.; Zanetti, G. D.; Irazoqui, Fernando Jose; Sendra, Victor German; Zlocowski, Natacha; et al.; Staining tumor cells with biotinylated ACL-I, a lectin isolated from the marine sponge, Axinella corrugata; Taylor & Francis; Biotechnic And Histochemistry; 88; 1; 9-2012; 1-91052-0295CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3109/10520295.2012.717304info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.3109/10520295.2012.717304info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:19Zoai:ri.conicet.gov.ar:11336/24985instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:19.958CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Staining tumor cells with biotinylated ACL-I, a lectin isolated from the marine sponge, Axinella corrugata
title Staining tumor cells with biotinylated ACL-I, a lectin isolated from the marine sponge, Axinella corrugata
spellingShingle Staining tumor cells with biotinylated ACL-I, a lectin isolated from the marine sponge, Axinella corrugata
Dresch, R. R.
Axinella Corrugata
Cancer
Carbohydrate
Lectin
title_short Staining tumor cells with biotinylated ACL-I, a lectin isolated from the marine sponge, Axinella corrugata
title_full Staining tumor cells with biotinylated ACL-I, a lectin isolated from the marine sponge, Axinella corrugata
title_fullStr Staining tumor cells with biotinylated ACL-I, a lectin isolated from the marine sponge, Axinella corrugata
title_full_unstemmed Staining tumor cells with biotinylated ACL-I, a lectin isolated from the marine sponge, Axinella corrugata
title_sort Staining tumor cells with biotinylated ACL-I, a lectin isolated from the marine sponge, Axinella corrugata
dc.creator.none.fl_str_mv Dresch, R. R.
Zanetti, G. D.
Irazoqui, Fernando Jose
Sendra, Victor German
Zlocowski, Natacha
Bernardi, A.
Rosa, R. M.
Battastini, A. M. O.
Henriques, Amalia Teresinha
Vozári Hampe, M. M.
author Dresch, R. R.
author_facet Dresch, R. R.
Zanetti, G. D.
Irazoqui, Fernando Jose
Sendra, Victor German
Zlocowski, Natacha
Bernardi, A.
Rosa, R. M.
Battastini, A. M. O.
Henriques, Amalia Teresinha
Vozári Hampe, M. M.
author_role author
author2 Zanetti, G. D.
Irazoqui, Fernando Jose
Sendra, Victor German
Zlocowski, Natacha
Bernardi, A.
Rosa, R. M.
Battastini, A. M. O.
Henriques, Amalia Teresinha
Vozári Hampe, M. M.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Axinella Corrugata
Cancer
Carbohydrate
Lectin
topic Axinella Corrugata
Cancer
Carbohydrate
Lectin
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Axinella corrugata lectin 1 (ACL-1) was purifi ed from aqueous extracts of the marine sponge, Axinella corrugata . ACL-1 strongly agglutinates native rabbit erythrocytes. The hemagglutination is inhibited by N -acetyl derivatives, particularly N , N ’ , N ” -triacetylchitotriose, N -acetyl-Dglucosamine, N -acetyl-D-mannosamine and N -acetyl-D-galactosamine. We investigated the capacity of biotinylated ACL-1 to stain several transformed cell lines including breast (T-47D, MCF7), colon (HT-29), lung (H460), ovary (OVCAR-3) and bladder (T24). ACL-I may bind to both monosaccharides and oligosaccharides of tumor cells, N -acetyl-D-galactosamine, and N -acetyl-Dglucosamine glycan types. The lectins are useful, not only as markers and diagnostic parameters, but also for tissue mapping in suspicious neoplasms. In addition, they provide a better understanding of neoplasms at the cytological and molecular levels. Furthermore, the use of potential metastatic markers such as lectins is crucial for developing successful tools for therapy against cancer. We observed that biotinylated ACL-I stains tumor cells and may hold potential as a probe for identifying transformed cells and for studying glycan structures synthesized by such cells.
Fil: Dresch, R. R.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Zanetti, G. D.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Irazoqui, Fernando Jose. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Quimica Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sendra, Victor German. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Quimica Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zlocowski, Natacha. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Quimica Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bernardi, A.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Rosa, R. M.. Universidade Luterana do Brasil; Brasil
Fil: Battastini, A. M. O.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Henriques, Amalia Teresinha. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Vozári Hampe, M. M.. Universidade Federal do Rio Grande do Sul; Brasil
description Axinella corrugata lectin 1 (ACL-1) was purifi ed from aqueous extracts of the marine sponge, Axinella corrugata . ACL-1 strongly agglutinates native rabbit erythrocytes. The hemagglutination is inhibited by N -acetyl derivatives, particularly N , N ’ , N ” -triacetylchitotriose, N -acetyl-Dglucosamine, N -acetyl-D-mannosamine and N -acetyl-D-galactosamine. We investigated the capacity of biotinylated ACL-1 to stain several transformed cell lines including breast (T-47D, MCF7), colon (HT-29), lung (H460), ovary (OVCAR-3) and bladder (T24). ACL-I may bind to both monosaccharides and oligosaccharides of tumor cells, N -acetyl-D-galactosamine, and N -acetyl-Dglucosamine glycan types. The lectins are useful, not only as markers and diagnostic parameters, but also for tissue mapping in suspicious neoplasms. In addition, they provide a better understanding of neoplasms at the cytological and molecular levels. Furthermore, the use of potential metastatic markers such as lectins is crucial for developing successful tools for therapy against cancer. We observed that biotinylated ACL-I stains tumor cells and may hold potential as a probe for identifying transformed cells and for studying glycan structures synthesized by such cells.
publishDate 2012
dc.date.none.fl_str_mv 2012-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24985
Dresch, R. R.; Zanetti, G. D.; Irazoqui, Fernando Jose; Sendra, Victor German; Zlocowski, Natacha; et al.; Staining tumor cells with biotinylated ACL-I, a lectin isolated from the marine sponge, Axinella corrugata; Taylor & Francis; Biotechnic And Histochemistry; 88; 1; 9-2012; 1-9
1052-0295
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24985
identifier_str_mv Dresch, R. R.; Zanetti, G. D.; Irazoqui, Fernando Jose; Sendra, Victor German; Zlocowski, Natacha; et al.; Staining tumor cells with biotinylated ACL-I, a lectin isolated from the marine sponge, Axinella corrugata; Taylor & Francis; Biotechnic And Histochemistry; 88; 1; 9-2012; 1-9
1052-0295
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3109/10520295.2012.717304
info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.3109/10520295.2012.717304
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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