Sustainable Bioremediation of Lipid-Rich Wastewater Using a Lipase from Penicillium rubens LBM 081

Autores
Ortellado, Laura Ester; Ayala Schimpf, Alan Rolando; Benitez, Silvana Florencia; Villalba, Laura Lidia; Zapata, Pedro Dario; Fonseca, Maria Isabel
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The improper discharge of wastewater has increased the presence of pollutants,among which lipids are particularly problematic. These compounds form oily layers thathinder oxygen transfer and sunlight penetration, negatively impacting aquatic ecosystems.Conventional methods for treating such effluents are often costly and environmentallyunfriendly. In this context, bioremediation using lipases, such as those produced byPenicillium rubens LBM 081, represents an effective and sustainable alternative. This studyevaluated the biotechnological potential of the lipase from P. rubens LBM 081 for the hydrolysisof lipid-rich wastewater. Lipase activity was influenced by the carbon and nitrogensources in the culture medium, reaching maximum activity (2780 U mL⁻1) under optimalconditions of 2% meat peptone, 4% olive oil, a spore concentration of 1 × 106, incubationat 30 °C, and agitation at 140 rpm. The optimized enzymatic supernatant significantlyreduced COD, oils, and total fats in the effluents. Furthermore, GC-MS analysisrevealed a significant increase in free fatty acids, confirming triglyceride hydrolysis. Theseresults highlight the potential of P. rubens LBM 081 lipase as an effective and environmentallysustainable biotechnological alternative for the treatment of lipid-rich wastewater.
Fil: Ortellado, Laura Ester. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Ayala Schimpf, Alan Rolando. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Benitez, Silvana Florencia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Villalba, Laura Lidia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Zapata, Pedro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Fonseca, Maria Isabel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Materia
FUNGAL LIPASE
ENZYMATIC BIOREMEDIATION
LIPID-RICH WASTEWATER TREATMENT
FAT AND OIL REMOVAL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/272334
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/272334
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sustainable Bioremediation of Lipid-Rich Wastewater Using a Lipase from Penicillium rubens LBM 081Ortellado, Laura EsterAyala Schimpf, Alan RolandoBenitez, Silvana FlorenciaVillalba, Laura LidiaZapata, Pedro DarioFonseca, Maria IsabelFUNGAL LIPASEENZYMATIC BIOREMEDIATIONLIPID-RICH WASTEWATER TREATMENTFAT AND OIL REMOVALhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2The improper discharge of wastewater has increased the presence of pollutants,among which lipids are particularly problematic. These compounds form oily layers thathinder oxygen transfer and sunlight penetration, negatively impacting aquatic ecosystems.Conventional methods for treating such effluents are often costly and environmentallyunfriendly. In this context, bioremediation using lipases, such as those produced byPenicillium rubens LBM 081, represents an effective and sustainable alternative. This studyevaluated the biotechnological potential of the lipase from P. rubens LBM 081 for the hydrolysisof lipid-rich wastewater. Lipase activity was influenced by the carbon and nitrogensources in the culture medium, reaching maximum activity (2780 U mL⁻1) under optimalconditions of 2% meat peptone, 4% olive oil, a spore concentration of 1 × 106, incubationat 30 °C, and agitation at 140 rpm. The optimized enzymatic supernatant significantlyreduced COD, oils, and total fats in the effluents. Furthermore, GC-MS analysisrevealed a significant increase in free fatty acids, confirming triglyceride hydrolysis. Theseresults highlight the potential of P. rubens LBM 081 lipase as an effective and environmentallysustainable biotechnological alternative for the treatment of lipid-rich wastewater.Fil: Ortellado, Laura Ester. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Ayala Schimpf, Alan Rolando. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Benitez, Silvana Florencia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Villalba, Laura Lidia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Zapata, Pedro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Fonseca, Maria Isabel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaMDPI2025-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/272334Ortellado, Laura Ester; Ayala Schimpf, Alan Rolando; Benitez, Silvana Florencia; Villalba, Laura Lidia; Zapata, Pedro Dario; et al.; Sustainable Bioremediation of Lipid-Rich Wastewater Using a Lipase from Penicillium rubens LBM 081; MDPI; Water; 17; 10; 5-2025; 1-272073-4441CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4441/17/10/1509info:eu-repo/semantics/altIdentifier/doi/10.3390/w17101509info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:47:33Zoai:ri.conicet.gov.ar:11336/272334instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:47:34.075CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sustainable Bioremediation of Lipid-Rich Wastewater Using a Lipase from Penicillium rubens LBM 081
title Sustainable Bioremediation of Lipid-Rich Wastewater Using a Lipase from Penicillium rubens LBM 081
spellingShingle Sustainable Bioremediation of Lipid-Rich Wastewater Using a Lipase from Penicillium rubens LBM 081
Ortellado, Laura Ester
FUNGAL LIPASE
ENZYMATIC BIOREMEDIATION
LIPID-RICH WASTEWATER TREATMENT
FAT AND OIL REMOVAL
title_short Sustainable Bioremediation of Lipid-Rich Wastewater Using a Lipase from Penicillium rubens LBM 081
title_full Sustainable Bioremediation of Lipid-Rich Wastewater Using a Lipase from Penicillium rubens LBM 081
title_fullStr Sustainable Bioremediation of Lipid-Rich Wastewater Using a Lipase from Penicillium rubens LBM 081
title_full_unstemmed Sustainable Bioremediation of Lipid-Rich Wastewater Using a Lipase from Penicillium rubens LBM 081
title_sort Sustainable Bioremediation of Lipid-Rich Wastewater Using a Lipase from Penicillium rubens LBM 081
dc.creator.none.fl_str_mv Ortellado, Laura Ester
Ayala Schimpf, Alan Rolando
Benitez, Silvana Florencia
Villalba, Laura Lidia
Zapata, Pedro Dario
Fonseca, Maria Isabel
author Ortellado, Laura Ester
author_facet Ortellado, Laura Ester
Ayala Schimpf, Alan Rolando
Benitez, Silvana Florencia
Villalba, Laura Lidia
Zapata, Pedro Dario
Fonseca, Maria Isabel
author_role author
author2 Ayala Schimpf, Alan Rolando
Benitez, Silvana Florencia
Villalba, Laura Lidia
Zapata, Pedro Dario
Fonseca, Maria Isabel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv FUNGAL LIPASE
ENZYMATIC BIOREMEDIATION
LIPID-RICH WASTEWATER TREATMENT
FAT AND OIL REMOVAL
topic FUNGAL LIPASE
ENZYMATIC BIOREMEDIATION
LIPID-RICH WASTEWATER TREATMENT
FAT AND OIL REMOVAL
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The improper discharge of wastewater has increased the presence of pollutants,among which lipids are particularly problematic. These compounds form oily layers thathinder oxygen transfer and sunlight penetration, negatively impacting aquatic ecosystems.Conventional methods for treating such effluents are often costly and environmentallyunfriendly. In this context, bioremediation using lipases, such as those produced byPenicillium rubens LBM 081, represents an effective and sustainable alternative. This studyevaluated the biotechnological potential of the lipase from P. rubens LBM 081 for the hydrolysisof lipid-rich wastewater. Lipase activity was influenced by the carbon and nitrogensources in the culture medium, reaching maximum activity (2780 U mL⁻1) under optimalconditions of 2% meat peptone, 4% olive oil, a spore concentration of 1 × 106, incubationat 30 °C, and agitation at 140 rpm. The optimized enzymatic supernatant significantlyreduced COD, oils, and total fats in the effluents. Furthermore, GC-MS analysisrevealed a significant increase in free fatty acids, confirming triglyceride hydrolysis. Theseresults highlight the potential of P. rubens LBM 081 lipase as an effective and environmentallysustainable biotechnological alternative for the treatment of lipid-rich wastewater.
Fil: Ortellado, Laura Ester. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Ayala Schimpf, Alan Rolando. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Benitez, Silvana Florencia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Villalba, Laura Lidia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Zapata, Pedro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Fonseca, Maria Isabel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
description The improper discharge of wastewater has increased the presence of pollutants,among which lipids are particularly problematic. These compounds form oily layers thathinder oxygen transfer and sunlight penetration, negatively impacting aquatic ecosystems.Conventional methods for treating such effluents are often costly and environmentallyunfriendly. In this context, bioremediation using lipases, such as those produced byPenicillium rubens LBM 081, represents an effective and sustainable alternative. This studyevaluated the biotechnological potential of the lipase from P. rubens LBM 081 for the hydrolysisof lipid-rich wastewater. Lipase activity was influenced by the carbon and nitrogensources in the culture medium, reaching maximum activity (2780 U mL⁻1) under optimalconditions of 2% meat peptone, 4% olive oil, a spore concentration of 1 × 106, incubationat 30 °C, and agitation at 140 rpm. The optimized enzymatic supernatant significantlyreduced COD, oils, and total fats in the effluents. Furthermore, GC-MS analysisrevealed a significant increase in free fatty acids, confirming triglyceride hydrolysis. Theseresults highlight the potential of P. rubens LBM 081 lipase as an effective and environmentallysustainable biotechnological alternative for the treatment of lipid-rich wastewater.
publishDate 2025
dc.date.none.fl_str_mv 2025-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/272334
Ortellado, Laura Ester; Ayala Schimpf, Alan Rolando; Benitez, Silvana Florencia; Villalba, Laura Lidia; Zapata, Pedro Dario; et al.; Sustainable Bioremediation of Lipid-Rich Wastewater Using a Lipase from Penicillium rubens LBM 081; MDPI; Water; 17; 10; 5-2025; 1-27
2073-4441
CONICET Digital
CONICET
url http://hdl.handle.net/11336/272334
identifier_str_mv Ortellado, Laura Ester; Ayala Schimpf, Alan Rolando; Benitez, Silvana Florencia; Villalba, Laura Lidia; Zapata, Pedro Dario; et al.; Sustainable Bioremediation of Lipid-Rich Wastewater Using a Lipase from Penicillium rubens LBM 081; MDPI; Water; 17; 10; 5-2025; 1-27
2073-4441
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4441/17/10/1509
info:eu-repo/semantics/altIdentifier/doi/10.3390/w17101509
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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