Screening of decolourizing yeasts with dye assimilation ability from Las Yungas rainforest

Autores
Martorell, María Martha; Pajot, Hipolito Fernando; Fariña, Julia Ines; Castellanos, Lucia Ines
Año de publicación
2010
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Environmental pollution by textile-dye containing wastewaters is a matter of worldwide concern. Yeasts bioremediation, a poorly explored and less exploited methodology, stands out as an interesting approach supported on their metabolic diversity and high growth rates. In this work, a screening protocol based on dye tolerance and dye assimilation ability on solid media was performed with a 2000 mg/L dye mixture. Thirty-nine yeast isolates from “Laurel del Monte” underlying soils from “Las Yungas” (Tucumán, Argentina) were obtained. Based on decolourization haloes and colony dyeing, 15 isolates showed the highest decolourization ability on agar plates containing Vilmafix® Blue RR-BB, Vilmafix® Red 7B-HE, Vilmafix® Black B-V and Vilmafix® Yellow 4R-HE, either alone or as a mixture. Screening on agar plates with synthetic media supplemented with each dye as sole C or N source, led to the selection of 10 isolates. Microsatellite RAPD analysis enabled the discrimination of these isolates into six groups. Following tests consisted on the analysis of growth and decolourization kinetic profiles, along with the production of Manganese peroxidase, laccase and tyrosinase activities in dye-supplemented (200 mg/L) liquid media. Time-dependent supernatant spectra, growth curves and enzymatic activities led to the selection of the two most promising isolates for future experiments
Fil: Martorell, María Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pajot, Hipolito Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Castellanos, Lucia Ines. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XLVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Puerto Madryn
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
Yeasts
Dyes
Yungas
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/193062
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/193062
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Screening of decolourizing yeasts with dye assimilation ability from Las Yungas rainforestMartorell, María MarthaPajot, Hipolito FernandoFariña, Julia InesCastellanos, Lucia InesYeastsDyesYungashttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Environmental pollution by textile-dye containing wastewaters is a matter of worldwide concern. Yeasts bioremediation, a poorly explored and less exploited methodology, stands out as an interesting approach supported on their metabolic diversity and high growth rates. In this work, a screening protocol based on dye tolerance and dye assimilation ability on solid media was performed with a 2000 mg/L dye mixture. Thirty-nine yeast isolates from “Laurel del Monte” underlying soils from “Las Yungas” (Tucumán, Argentina) were obtained. Based on decolourization haloes and colony dyeing, 15 isolates showed the highest decolourization ability on agar plates containing Vilmafix® Blue RR-BB, Vilmafix® Red 7B-HE, Vilmafix® Black B-V and Vilmafix® Yellow 4R-HE, either alone or as a mixture. Screening on agar plates with synthetic media supplemented with each dye as sole C or N source, led to the selection of 10 isolates. Microsatellite RAPD analysis enabled the discrimination of these isolates into six groups. Following tests consisted on the analysis of growth and decolourization kinetic profiles, along with the production of Manganese peroxidase, laccase and tyrosinase activities in dye-supplemented (200 mg/L) liquid media. Time-dependent supernatant spectra, growth curves and enzymatic activities led to the selection of the two most promising isolates for future experimentsFil: Martorell, María Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Pajot, Hipolito Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Castellanos, Lucia Ines. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaXLVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología MolecularPuerto MadrynArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularSociedad Argentina de Investigación en Bioquímica y Biología Molecular2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/193062Screening of decolourizing yeasts with dye assimilation ability from Las Yungas rainforest; XLVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Puerto Madryn; Argentina; 2010; 1-21667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-34.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:52Zoai:ri.conicet.gov.ar:11336/193062instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:53.25CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Screening of decolourizing yeasts with dye assimilation ability from Las Yungas rainforest
title Screening of decolourizing yeasts with dye assimilation ability from Las Yungas rainforest
spellingShingle Screening of decolourizing yeasts with dye assimilation ability from Las Yungas rainforest
Martorell, María Martha
Yeasts
Dyes
Yungas
title_short Screening of decolourizing yeasts with dye assimilation ability from Las Yungas rainforest
title_full Screening of decolourizing yeasts with dye assimilation ability from Las Yungas rainforest
title_fullStr Screening of decolourizing yeasts with dye assimilation ability from Las Yungas rainforest
title_full_unstemmed Screening of decolourizing yeasts with dye assimilation ability from Las Yungas rainforest
title_sort Screening of decolourizing yeasts with dye assimilation ability from Las Yungas rainforest
dc.creator.none.fl_str_mv Martorell, María Martha
Pajot, Hipolito Fernando
Fariña, Julia Ines
Castellanos, Lucia Ines
author Martorell, María Martha
author_facet Martorell, María Martha
Pajot, Hipolito Fernando
Fariña, Julia Ines
Castellanos, Lucia Ines
author_role author
author2 Pajot, Hipolito Fernando
Fariña, Julia Ines
Castellanos, Lucia Ines
author2_role author
author
author
dc.subject.none.fl_str_mv Yeasts
Dyes
Yungas
topic Yeasts
Dyes
Yungas
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Environmental pollution by textile-dye containing wastewaters is a matter of worldwide concern. Yeasts bioremediation, a poorly explored and less exploited methodology, stands out as an interesting approach supported on their metabolic diversity and high growth rates. In this work, a screening protocol based on dye tolerance and dye assimilation ability on solid media was performed with a 2000 mg/L dye mixture. Thirty-nine yeast isolates from “Laurel del Monte” underlying soils from “Las Yungas” (Tucumán, Argentina) were obtained. Based on decolourization haloes and colony dyeing, 15 isolates showed the highest decolourization ability on agar plates containing Vilmafix® Blue RR-BB, Vilmafix® Red 7B-HE, Vilmafix® Black B-V and Vilmafix® Yellow 4R-HE, either alone or as a mixture. Screening on agar plates with synthetic media supplemented with each dye as sole C or N source, led to the selection of 10 isolates. Microsatellite RAPD analysis enabled the discrimination of these isolates into six groups. Following tests consisted on the analysis of growth and decolourization kinetic profiles, along with the production of Manganese peroxidase, laccase and tyrosinase activities in dye-supplemented (200 mg/L) liquid media. Time-dependent supernatant spectra, growth curves and enzymatic activities led to the selection of the two most promising isolates for future experiments
Fil: Martorell, María Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pajot, Hipolito Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Castellanos, Lucia Ines. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XLVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Puerto Madryn
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description Environmental pollution by textile-dye containing wastewaters is a matter of worldwide concern. Yeasts bioremediation, a poorly explored and less exploited methodology, stands out as an interesting approach supported on their metabolic diversity and high growth rates. In this work, a screening protocol based on dye tolerance and dye assimilation ability on solid media was performed with a 2000 mg/L dye mixture. Thirty-nine yeast isolates from “Laurel del Monte” underlying soils from “Las Yungas” (Tucumán, Argentina) were obtained. Based on decolourization haloes and colony dyeing, 15 isolates showed the highest decolourization ability on agar plates containing Vilmafix® Blue RR-BB, Vilmafix® Red 7B-HE, Vilmafix® Black B-V and Vilmafix® Yellow 4R-HE, either alone or as a mixture. Screening on agar plates with synthetic media supplemented with each dye as sole C or N source, led to the selection of 10 isolates. Microsatellite RAPD analysis enabled the discrimination of these isolates into six groups. Following tests consisted on the analysis of growth and decolourization kinetic profiles, along with the production of Manganese peroxidase, laccase and tyrosinase activities in dye-supplemented (200 mg/L) liquid media. Time-dependent supernatant spectra, growth curves and enzymatic activities led to the selection of the two most promising isolates for future experiments
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/193062
Screening of decolourizing yeasts with dye assimilation ability from Las Yungas rainforest; XLVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Puerto Madryn; Argentina; 2010; 1-2
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/193062
identifier_str_mv Screening of decolourizing yeasts with dye assimilation ability from Las Yungas rainforest; XLVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Puerto Madryn; Argentina; 2010; 1-2
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-34.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
publisher.none.fl_str_mv Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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