In Vivo Crosslinking Methods for Analyzing the Assembly and Architecture of Chemoreceptor Arrays

• Autores: Studdert, Claudia Alicia; Parkinson, John S.
• Año de publicación: 2007
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: CHEMORECEPTORS; IN VIVOCROSSLINKING
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

The chemoreceptor molecules that mediate chemotactic responses in bacteria and archaea are physically clustered and operate as highly cooperative arrays. Few experimental approaches are able to investigate the structure-function organization of these chemoreceptor networks in living cells. This chapter describes chemical crosslinking methods that can be applied under normal physiological conditions to explore physical interactions between chemoreceptors and their underlying genetic and structural basis. Most of these crosslinking approaches are based on available atomic structures for chemoreceptor homodimers, the fundamental building block for higher-order networks. However, the general logic of our in vivo crosslinking approaches is readily applicable to other protein-protein interactions and other organisms, even when high-resolution structural information is not available.
Fil: Studdert, Claudia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Parkinson, John S.. No especifíca;