Introducing the concept of biocatalysis in the classroom: The conversion of cholesterol to provitamin D 3

Autores
De Luca, Belén M.; Nudel, Berta Clara; Gonzalez, Rodrigo Horacio; Nusblat, Alejandro David
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Biocatalysis is a fundamental concept in biotechnology. The topic integrates knowledge of several disciplines; therefore, it was included in the course “design and optimization of biological systems” which is offered in the biochemistry curricula. We selected the ciliate tetrahymena as an example of a eukaryotic system with potential for the biotransformation of sterol metabolites of industrial interest; in particular, we focused on the conversion of cholesterol to provitamin D3. The students work with wild type and recombinant strains and learn how sterol pathways could be modified to obtain diverse sterol moieties. During the course the students identify and measure the concentration of sterols. They also search for related genes by bioinformatic analysis. Additionally, the students compare biotransformation rates, growing the ciliate in plate and in a bioreactor. Finally, they use fluorescence microscopy to localize an enzyme involved in biotransformation. The last day each team makes an oral presentation, explaining the results obtained and responds to a series of key questions posed by the teachers, which determine the final mark. In our experience, this course enables undergraduate students to become acquainted with the principles of biocatalysis as well as with standard and modern techniques, through a simple and robust laboratory exercise, using a biological system for the conversion of valuable pharmaceutical moieties.
Fil: De Luca, Belén M.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Nudel, Berta Clara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Gonzalez, Rodrigo Horacio. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Nusblat, Alejandro David. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Materia
Tetrahymena
classroom
biotransformation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47468
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Introducing the concept of biocatalysis in the classroom: The conversion of cholesterol to provitamin D 3De Luca, Belén M.Nudel, Berta ClaraGonzalez, Rodrigo HoracioNusblat, Alejandro DavidTetrahymenaclassroombiotransformationhttps://purl.org/becyt/ford/5.3https://purl.org/becyt/ford/5Biocatalysis is a fundamental concept in biotechnology. The topic integrates knowledge of several disciplines; therefore, it was included in the course “design and optimization of biological systems” which is offered in the biochemistry curricula. We selected the ciliate tetrahymena as an example of a eukaryotic system with potential for the biotransformation of sterol metabolites of industrial interest; in particular, we focused on the conversion of cholesterol to provitamin D3. The students work with wild type and recombinant strains and learn how sterol pathways could be modified to obtain diverse sterol moieties. During the course the students identify and measure the concentration of sterols. They also search for related genes by bioinformatic analysis. Additionally, the students compare biotransformation rates, growing the ciliate in plate and in a bioreactor. Finally, they use fluorescence microscopy to localize an enzyme involved in biotransformation. The last day each team makes an oral presentation, explaining the results obtained and responds to a series of key questions posed by the teachers, which determine the final mark. In our experience, this course enables undergraduate students to become acquainted with the principles of biocatalysis as well as with standard and modern techniques, through a simple and robust laboratory exercise, using a biological system for the conversion of valuable pharmaceutical moieties.Fil: De Luca, Belén M.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Nudel, Berta Clara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Gonzalez, Rodrigo Horacio. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Nusblat, Alejandro David. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaJohn Wiley & Sons Inc2017-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47468De Luca, Belén M.; Nudel, Berta Clara; Gonzalez, Rodrigo Horacio; Nusblat, Alejandro David; Introducing the concept of biocatalysis in the classroom: The conversion of cholesterol to provitamin D 3; John Wiley & Sons Inc; Biochemistry and Molecular Biology Education; 45; 2; 3-2017; 105-1141470-8175CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/bmb.20995info:eu-repo/semantics/altIdentifier/url/https://iubmb.onlinelibrary.wiley.com/doi/abs/10.1002/bmb.20995info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:17:10Zoai:ri.conicet.gov.ar:11336/47468instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:17:10.789CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Introducing the concept of biocatalysis in the classroom: The conversion of cholesterol to provitamin D 3
title Introducing the concept of biocatalysis in the classroom: The conversion of cholesterol to provitamin D 3
spellingShingle Introducing the concept of biocatalysis in the classroom: The conversion of cholesterol to provitamin D 3
De Luca, Belén M.
Tetrahymena
classroom
biotransformation
title_short Introducing the concept of biocatalysis in the classroom: The conversion of cholesterol to provitamin D 3
title_full Introducing the concept of biocatalysis in the classroom: The conversion of cholesterol to provitamin D 3
title_fullStr Introducing the concept of biocatalysis in the classroom: The conversion of cholesterol to provitamin D 3
title_full_unstemmed Introducing the concept of biocatalysis in the classroom: The conversion of cholesterol to provitamin D 3
title_sort Introducing the concept of biocatalysis in the classroom: The conversion of cholesterol to provitamin D 3
dc.creator.none.fl_str_mv De Luca, Belén M.
Nudel, Berta Clara
Gonzalez, Rodrigo Horacio
Nusblat, Alejandro David
author De Luca, Belén M.
author_facet De Luca, Belén M.
Nudel, Berta Clara
Gonzalez, Rodrigo Horacio
Nusblat, Alejandro David
author_role author
author2 Nudel, Berta Clara
Gonzalez, Rodrigo Horacio
Nusblat, Alejandro David
author2_role author
author
author
dc.subject.none.fl_str_mv Tetrahymena
classroom
biotransformation
topic Tetrahymena
classroom
biotransformation
purl_subject.fl_str_mv https://purl.org/becyt/ford/5.3
https://purl.org/becyt/ford/5
dc.description.none.fl_txt_mv Biocatalysis is a fundamental concept in biotechnology. The topic integrates knowledge of several disciplines; therefore, it was included in the course “design and optimization of biological systems” which is offered in the biochemistry curricula. We selected the ciliate tetrahymena as an example of a eukaryotic system with potential for the biotransformation of sterol metabolites of industrial interest; in particular, we focused on the conversion of cholesterol to provitamin D3. The students work with wild type and recombinant strains and learn how sterol pathways could be modified to obtain diverse sterol moieties. During the course the students identify and measure the concentration of sterols. They also search for related genes by bioinformatic analysis. Additionally, the students compare biotransformation rates, growing the ciliate in plate and in a bioreactor. Finally, they use fluorescence microscopy to localize an enzyme involved in biotransformation. The last day each team makes an oral presentation, explaining the results obtained and responds to a series of key questions posed by the teachers, which determine the final mark. In our experience, this course enables undergraduate students to become acquainted with the principles of biocatalysis as well as with standard and modern techniques, through a simple and robust laboratory exercise, using a biological system for the conversion of valuable pharmaceutical moieties.
Fil: De Luca, Belén M.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Nudel, Berta Clara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Gonzalez, Rodrigo Horacio. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Nusblat, Alejandro David. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
description Biocatalysis is a fundamental concept in biotechnology. The topic integrates knowledge of several disciplines; therefore, it was included in the course “design and optimization of biological systems” which is offered in the biochemistry curricula. We selected the ciliate tetrahymena as an example of a eukaryotic system with potential for the biotransformation of sterol metabolites of industrial interest; in particular, we focused on the conversion of cholesterol to provitamin D3. The students work with wild type and recombinant strains and learn how sterol pathways could be modified to obtain diverse sterol moieties. During the course the students identify and measure the concentration of sterols. They also search for related genes by bioinformatic analysis. Additionally, the students compare biotransformation rates, growing the ciliate in plate and in a bioreactor. Finally, they use fluorescence microscopy to localize an enzyme involved in biotransformation. The last day each team makes an oral presentation, explaining the results obtained and responds to a series of key questions posed by the teachers, which determine the final mark. In our experience, this course enables undergraduate students to become acquainted with the principles of biocatalysis as well as with standard and modern techniques, through a simple and robust laboratory exercise, using a biological system for the conversion of valuable pharmaceutical moieties.
publishDate 2017
dc.date.none.fl_str_mv 2017-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47468
De Luca, Belén M.; Nudel, Berta Clara; Gonzalez, Rodrigo Horacio; Nusblat, Alejandro David; Introducing the concept of biocatalysis in the classroom: The conversion of cholesterol to provitamin D 3; John Wiley & Sons Inc; Biochemistry and Molecular Biology Education; 45; 2; 3-2017; 105-114
1470-8175
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47468
identifier_str_mv De Luca, Belén M.; Nudel, Berta Clara; Gonzalez, Rodrigo Horacio; Nusblat, Alejandro David; Introducing the concept of biocatalysis in the classroom: The conversion of cholesterol to provitamin D 3; John Wiley & Sons Inc; Biochemistry and Molecular Biology Education; 45; 2; 3-2017; 105-114
1470-8175
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/bmb.20995
info:eu-repo/semantics/altIdentifier/url/https://iubmb.onlinelibrary.wiley.com/doi/abs/10.1002/bmb.20995
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Inc
publisher.none.fl_str_mv John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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