Bioconversion of egg cholesterol to pro-vitamin D sterols with Tetrahymena thermophila

Autores
Valcarce, G.; Nusblat, Alejandro David; Florin-Christensen, Jorge; Nudel, Berta Clara
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The potential use of the non-pathogenic ciliate Tetrahymena thermophila for converting cholesterol into pro-vitamin D sterols in egg suspensions was investigated. Conditions screened for efficient conversion included inoculum size and age, egg and egg yolk dilution, initial pH, and aeration. By optimizing these parameters, 55% ± 6 cholesterol reduction was obtained in 24 hours incubation. Tetrahymena converted cholesterol into three unsaturated sterols, D7, 22 bis-dehydrocholesterol, an ergosterol analog (27%), D7-dehydrocholesterol, also known as pro-vitamin D3 (4%), and A22- dehydrocholesterol (8%). Despite significant proteolysis, culturing with Tetrahymena did not result in net consumption of total amino acids content, which was in fact increased by 16% ± 2. These findings emphasize the potential use of Tetrahymena in the food industry.
Fil: Valcarce, G.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Nusblat, Alejandro David. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Florin-Christensen, Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Nudel, Berta Clara. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Materia
CHOLESTEROL BIOCONVERSION
EGG
EGG YOLK SUSPENSIONS
PRO-VITAMIN D FORMATION
TETRAHYMENA THERMOPHILA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/162290
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/162290
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bioconversion of egg cholesterol to pro-vitamin D sterols with Tetrahymena thermophilaValcarce, G.Nusblat, Alejandro DavidFlorin-Christensen, JorgeNudel, Berta ClaraCHOLESTEROL BIOCONVERSIONEGGEGG YOLK SUSPENSIONSPRO-VITAMIN D FORMATIONTETRAHYMENA THERMOPHILAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The potential use of the non-pathogenic ciliate Tetrahymena thermophila for converting cholesterol into pro-vitamin D sterols in egg suspensions was investigated. Conditions screened for efficient conversion included inoculum size and age, egg and egg yolk dilution, initial pH, and aeration. By optimizing these parameters, 55% ± 6 cholesterol reduction was obtained in 24 hours incubation. Tetrahymena converted cholesterol into three unsaturated sterols, D7, 22 bis-dehydrocholesterol, an ergosterol analog (27%), D7-dehydrocholesterol, also known as pro-vitamin D3 (4%), and A22- dehydrocholesterol (8%). Despite significant proteolysis, culturing with Tetrahymena did not result in net consumption of total amino acids content, which was in fact increased by 16% ± 2. These findings emphasize the potential use of Tetrahymena in the food industry.Fil: Valcarce, G.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Nusblat, Alejandro David. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Florin-Christensen, Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Nudel, Berta Clara. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaWiley Blackwell Publishing, Inc2002-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/162290Valcarce, G.; Nusblat, Alejandro David; Florin-Christensen, Jorge; Nudel, Berta Clara; Bioconversion of egg cholesterol to pro-vitamin D sterols with Tetrahymena thermophila; Wiley Blackwell Publishing, Inc; Journal of Food Science; 67; 6; 8-2002; 2405-24090022-1147CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://ift.onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2621.2002.tb09561.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2621.2002.tb09561.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:42:44Zoai:ri.conicet.gov.ar:11336/162290instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:42:44.868CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bioconversion of egg cholesterol to pro-vitamin D sterols with Tetrahymena thermophila
title Bioconversion of egg cholesterol to pro-vitamin D sterols with Tetrahymena thermophila
spellingShingle Bioconversion of egg cholesterol to pro-vitamin D sterols with Tetrahymena thermophila
Valcarce, G.
CHOLESTEROL BIOCONVERSION
EGG
EGG YOLK SUSPENSIONS
PRO-VITAMIN D FORMATION
TETRAHYMENA THERMOPHILA
title_short Bioconversion of egg cholesterol to pro-vitamin D sterols with Tetrahymena thermophila
title_full Bioconversion of egg cholesterol to pro-vitamin D sterols with Tetrahymena thermophila
title_fullStr Bioconversion of egg cholesterol to pro-vitamin D sterols with Tetrahymena thermophila
title_full_unstemmed Bioconversion of egg cholesterol to pro-vitamin D sterols with Tetrahymena thermophila
title_sort Bioconversion of egg cholesterol to pro-vitamin D sterols with Tetrahymena thermophila
dc.creator.none.fl_str_mv Valcarce, G.
Nusblat, Alejandro David
Florin-Christensen, Jorge
Nudel, Berta Clara
author Valcarce, G.
author_facet Valcarce, G.
Nusblat, Alejandro David
Florin-Christensen, Jorge
Nudel, Berta Clara
author_role author
author2 Nusblat, Alejandro David
Florin-Christensen, Jorge
Nudel, Berta Clara
author2_role author
author
author
dc.subject.none.fl_str_mv CHOLESTEROL BIOCONVERSION
EGG
EGG YOLK SUSPENSIONS
PRO-VITAMIN D FORMATION
TETRAHYMENA THERMOPHILA
topic CHOLESTEROL BIOCONVERSION
EGG
EGG YOLK SUSPENSIONS
PRO-VITAMIN D FORMATION
TETRAHYMENA THERMOPHILA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The potential use of the non-pathogenic ciliate Tetrahymena thermophila for converting cholesterol into pro-vitamin D sterols in egg suspensions was investigated. Conditions screened for efficient conversion included inoculum size and age, egg and egg yolk dilution, initial pH, and aeration. By optimizing these parameters, 55% ± 6 cholesterol reduction was obtained in 24 hours incubation. Tetrahymena converted cholesterol into three unsaturated sterols, D7, 22 bis-dehydrocholesterol, an ergosterol analog (27%), D7-dehydrocholesterol, also known as pro-vitamin D3 (4%), and A22- dehydrocholesterol (8%). Despite significant proteolysis, culturing with Tetrahymena did not result in net consumption of total amino acids content, which was in fact increased by 16% ± 2. These findings emphasize the potential use of Tetrahymena in the food industry.
Fil: Valcarce, G.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Nusblat, Alejandro David. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Florin-Christensen, Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Nudel, Berta Clara. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
description The potential use of the non-pathogenic ciliate Tetrahymena thermophila for converting cholesterol into pro-vitamin D sterols in egg suspensions was investigated. Conditions screened for efficient conversion included inoculum size and age, egg and egg yolk dilution, initial pH, and aeration. By optimizing these parameters, 55% ± 6 cholesterol reduction was obtained in 24 hours incubation. Tetrahymena converted cholesterol into three unsaturated sterols, D7, 22 bis-dehydrocholesterol, an ergosterol analog (27%), D7-dehydrocholesterol, also known as pro-vitamin D3 (4%), and A22- dehydrocholesterol (8%). Despite significant proteolysis, culturing with Tetrahymena did not result in net consumption of total amino acids content, which was in fact increased by 16% ± 2. These findings emphasize the potential use of Tetrahymena in the food industry.
publishDate 2002
dc.date.none.fl_str_mv 2002-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/162290
Valcarce, G.; Nusblat, Alejandro David; Florin-Christensen, Jorge; Nudel, Berta Clara; Bioconversion of egg cholesterol to pro-vitamin D sterols with Tetrahymena thermophila; Wiley Blackwell Publishing, Inc; Journal of Food Science; 67; 6; 8-2002; 2405-2409
0022-1147
CONICET Digital
CONICET
url http://hdl.handle.net/11336/162290
identifier_str_mv Valcarce, G.; Nusblat, Alejandro David; Florin-Christensen, Jorge; Nudel, Berta Clara; Bioconversion of egg cholesterol to pro-vitamin D sterols with Tetrahymena thermophila; Wiley Blackwell Publishing, Inc; Journal of Food Science; 67; 6; 8-2002; 2405-2409
0022-1147
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ift.onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2621.2002.tb09561.x
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2621.2002.tb09561.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1847978127261696000
score 13.121305

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