Harmful Effects of the Dermal Intake of Commercial Formulations Containing Chlorpyrifos, 2,4-D, and Glyphosate on the Common Toad Rhinella arenarum (Anura: Bufonidae)

Autores: Lajmanovich, Rafael Carlos; Attademo, Andres Maximiliano; Simoniello, Maria Fernanda; Poletta, Gisela Laura; Junges, Celina Maria; Peltzer, Paola; Grenón, Paula; Cabagna Zenklusen, Mariana Cristina
Año de publicación: 2015
Idioma: español castellano
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Amphibians have complex life cycles with aquatic and terrestrial life and uncovered skins; therefore, they are exposed to chemical contamination, where dermal exposure is a significant route for pesticide uptake in both habitats. In this study, measurements in blood samples such as levels of butyrylcholinesterase (BChE), carboxylesterase (CbE), glutathione Stransferases (GST), thiobarbituric acid reactive substances (TBARS), modified alkaline comet assay (ACA) for detection of oxidized bases (FPG and Endo III sites), as well as the ratio of heterophils and lymphocytes (H/L), were evaluated as non-destructive biomarkers to monitor dermal pesticide exposure in male toads of Rhinella arenarum. Toads were exposed to a solution containing a nominal concentration of commercial formulations of the insecticide chlorpyrifos (CPF, 10 mg/L), and herbicides 2,4-D and glyphosate (GLY) (20 mg/L, respectively). After 48 h of exposure, the levels of plasma B-sterases (BChE and CbE) were inhibited (55 and 43 %, respectively) in toads exposed to CPF. Also, the activity of GST was inducted for dermal exposure to 2,4-D, as well as the levels of TBARS due to CPF exposure. Besides this, CPF and 2,4-D exposure induced oxidative DNA damage, and the H/L ratio decreased for the both herbicide exposures. Our results showed that exposure via dermal uptake to CPF, 2,4-D, and GLY in the common toad R. arenarum induced neurotoxicity, oxidative stress, and immunological depression. Thus, some blood biomarkers employed in our study (B-esterases, GST, levels of TBARS, ACA, and H/L ratio) might be used as predictors in health and ecological risk assessment of amphibian populations exposed to OP insecticides and herbicides.
Fil: Lajmanovich, Rafael Carlos. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Attademo, Andres Maximiliano. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Simoniello, Maria Fernanda. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Poletta, Gisela Laura. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Junges, Celina Maria. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Peltzer, Paola. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Grenón, Paula. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicologia; Argentina
Fil: Cabagna Zenklusen, Mariana Cristina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Materia: Dermal Exposure
Amphibians
Chlorpyrifos
2,4-D
Glyphosate
Blood Non-Destructive Parameters
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/17631

Acceder

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spelling	Harmful Effects of the Dermal Intake of Commercial Formulations Containing Chlorpyrifos, 2,4-D, and Glyphosate on the Common Toad Rhinella arenarum (Anura: Bufonidae)Lajmanovich, Rafael CarlosAttademo, Andres MaximilianoSimoniello, Maria FernandaPoletta, Gisela LauraJunges, Celina MariaPeltzer, PaolaGrenón, PaulaCabagna Zenklusen, Mariana CristinaDermal ExposureAmphibiansChlorpyrifos2,4-DGlyphosateBlood Non-Destructive Parametershttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Amphibians have complex life cycles with aquatic and terrestrial life and uncovered skins; therefore, they are exposed to chemical contamination, where dermal exposure is a significant route for pesticide uptake in both habitats. In this study, measurements in blood samples such as levels of butyrylcholinesterase (BChE), carboxylesterase (CbE), glutathione Stransferases (GST), thiobarbituric acid reactive substances (TBARS), modified alkaline comet assay (ACA) for detection of oxidized bases (FPG and Endo III sites), as well as the ratio of heterophils and lymphocytes (H/L), were evaluated as non-destructive biomarkers to monitor dermal pesticide exposure in male toads of Rhinella arenarum. Toads were exposed to a solution containing a nominal concentration of commercial formulations of the insecticide chlorpyrifos (CPF, 10 mg/L), and herbicides 2,4-D and glyphosate (GLY) (20 mg/L, respectively). After 48 h of exposure, the levels of plasma B-sterases (BChE and CbE) were inhibited (55 and 43 %, respectively) in toads exposed to CPF. Also, the activity of GST was inducted for dermal exposure to 2,4-D, as well as the levels of TBARS due to CPF exposure. Besides this, CPF and 2,4-D exposure induced oxidative DNA damage, and the H/L ratio decreased for the both herbicide exposures. Our results showed that exposure via dermal uptake to CPF, 2,4-D, and GLY in the common toad R. arenarum induced neurotoxicity, oxidative stress, and immunological depression. Thus, some blood biomarkers employed in our study (B-esterases, GST, levels of TBARS, ACA, and H/L ratio) might be used as predictors in health and ecological risk assessment of amphibian populations exposed to OP insecticides and herbicides.Fil: Lajmanovich, Rafael Carlos. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Attademo, Andres Maximiliano. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Simoniello, Maria Fernanda. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Poletta, Gisela Laura. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Junges, Celina Maria. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Peltzer, Paola. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Grenón, Paula. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicologia; ArgentinaFil: Cabagna Zenklusen, Mariana Cristina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaSpringer2015-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/17631Lajmanovich, Rafael Carlos; Attademo, Andres Maximiliano; Simoniello, Maria Fernanda; Poletta, Gisela Laura; Junges, Celina Maria; et al.; Harmful Effects of the Dermal Intake of Commercial Formulations Containing Chlorpyrifos, 2,4-D, and Glyphosate on the Common Toad Rhinella arenarum (Anura: Bufonidae); Springer; Water, Air and Soil Pollution; 226; 11-2015; 1-12;4270049-69791573-2932spainfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11270-015-2695-9info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11270-015-2695-9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:04Zoai:ri.conicet.gov.ar:11336/17631instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:04.543CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Harmful Effects of the Dermal Intake of Commercial Formulations Containing Chlorpyrifos, 2,4-D, and Glyphosate on the Common Toad Rhinella arenarum (Anura: Bufonidae)
title	Harmful Effects of the Dermal Intake of Commercial Formulations Containing Chlorpyrifos, 2,4-D, and Glyphosate on the Common Toad Rhinella arenarum (Anura: Bufonidae)
spellingShingle	Harmful Effects of the Dermal Intake of Commercial Formulations Containing Chlorpyrifos, 2,4-D, and Glyphosate on the Common Toad Rhinella arenarum (Anura: Bufonidae) Lajmanovich, Rafael Carlos Dermal Exposure Amphibians Chlorpyrifos 2,4-D Glyphosate Blood Non-Destructive Parameters
title_short	Harmful Effects of the Dermal Intake of Commercial Formulations Containing Chlorpyrifos, 2,4-D, and Glyphosate on the Common Toad Rhinella arenarum (Anura: Bufonidae)
title_full	Harmful Effects of the Dermal Intake of Commercial Formulations Containing Chlorpyrifos, 2,4-D, and Glyphosate on the Common Toad Rhinella arenarum (Anura: Bufonidae)
title_fullStr	Harmful Effects of the Dermal Intake of Commercial Formulations Containing Chlorpyrifos, 2,4-D, and Glyphosate on the Common Toad Rhinella arenarum (Anura: Bufonidae)
title_full_unstemmed	Harmful Effects of the Dermal Intake of Commercial Formulations Containing Chlorpyrifos, 2,4-D, and Glyphosate on the Common Toad Rhinella arenarum (Anura: Bufonidae)
title_sort	Harmful Effects of the Dermal Intake of Commercial Formulations Containing Chlorpyrifos, 2,4-D, and Glyphosate on the Common Toad Rhinella arenarum (Anura: Bufonidae)
dc.creator.none.fl_str_mv	Lajmanovich, Rafael Carlos Attademo, Andres Maximiliano Simoniello, Maria Fernanda Poletta, Gisela Laura Junges, Celina Maria Peltzer, Paola Grenón, Paula Cabagna Zenklusen, Mariana Cristina
author	Lajmanovich, Rafael Carlos
author_facet	Lajmanovich, Rafael Carlos Attademo, Andres Maximiliano Simoniello, Maria Fernanda Poletta, Gisela Laura Junges, Celina Maria Peltzer, Paola Grenón, Paula Cabagna Zenklusen, Mariana Cristina
author_role	author
author2	Attademo, Andres Maximiliano Simoniello, Maria Fernanda Poletta, Gisela Laura Junges, Celina Maria Peltzer, Paola Grenón, Paula Cabagna Zenklusen, Mariana Cristina
author2_role	author author author author author author author
dc.subject.none.fl_str_mv	Dermal Exposure Amphibians Chlorpyrifos 2,4-D Glyphosate Blood Non-Destructive Parameters
topic	Dermal Exposure Amphibians Chlorpyrifos 2,4-D Glyphosate Blood Non-Destructive Parameters
purl_subject.fl_str_mv	https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv	Amphibians have complex life cycles with aquatic and terrestrial life and uncovered skins; therefore, they are exposed to chemical contamination, where dermal exposure is a significant route for pesticide uptake in both habitats. In this study, measurements in blood samples such as levels of butyrylcholinesterase (BChE), carboxylesterase (CbE), glutathione Stransferases (GST), thiobarbituric acid reactive substances (TBARS), modified alkaline comet assay (ACA) for detection of oxidized bases (FPG and Endo III sites), as well as the ratio of heterophils and lymphocytes (H/L), were evaluated as non-destructive biomarkers to monitor dermal pesticide exposure in male toads of Rhinella arenarum. Toads were exposed to a solution containing a nominal concentration of commercial formulations of the insecticide chlorpyrifos (CPF, 10 mg/L), and herbicides 2,4-D and glyphosate (GLY) (20 mg/L, respectively). After 48 h of exposure, the levels of plasma B-sterases (BChE and CbE) were inhibited (55 and 43 %, respectively) in toads exposed to CPF. Also, the activity of GST was inducted for dermal exposure to 2,4-D, as well as the levels of TBARS due to CPF exposure. Besides this, CPF and 2,4-D exposure induced oxidative DNA damage, and the H/L ratio decreased for the both herbicide exposures. Our results showed that exposure via dermal uptake to CPF, 2,4-D, and GLY in the common toad R. arenarum induced neurotoxicity, oxidative stress, and immunological depression. Thus, some blood biomarkers employed in our study (B-esterases, GST, levels of TBARS, ACA, and H/L ratio) might be used as predictors in health and ecological risk assessment of amphibian populations exposed to OP insecticides and herbicides. Fil: Lajmanovich, Rafael Carlos. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina Fil: Attademo, Andres Maximiliano. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina Fil: Simoniello, Maria Fernanda. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Poletta, Gisela Laura. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina Fil: Junges, Celina Maria. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina Fil: Peltzer, Paola. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina Fil: Grenón, Paula. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicologia; Argentina Fil: Cabagna Zenklusen, Mariana Cristina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Saneamiento Ambiental. Cátedra de Ecotoxicologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
description	Amphibians have complex life cycles with aquatic and terrestrial life and uncovered skins; therefore, they are exposed to chemical contamination, where dermal exposure is a significant route for pesticide uptake in both habitats. In this study, measurements in blood samples such as levels of butyrylcholinesterase (BChE), carboxylesterase (CbE), glutathione Stransferases (GST), thiobarbituric acid reactive substances (TBARS), modified alkaline comet assay (ACA) for detection of oxidized bases (FPG and Endo III sites), as well as the ratio of heterophils and lymphocytes (H/L), were evaluated as non-destructive biomarkers to monitor dermal pesticide exposure in male toads of Rhinella arenarum. Toads were exposed to a solution containing a nominal concentration of commercial formulations of the insecticide chlorpyrifos (CPF, 10 mg/L), and herbicides 2,4-D and glyphosate (GLY) (20 mg/L, respectively). After 48 h of exposure, the levels of plasma B-sterases (BChE and CbE) were inhibited (55 and 43 %, respectively) in toads exposed to CPF. Also, the activity of GST was inducted for dermal exposure to 2,4-D, as well as the levels of TBARS due to CPF exposure. Besides this, CPF and 2,4-D exposure induced oxidative DNA damage, and the H/L ratio decreased for the both herbicide exposures. Our results showed that exposure via dermal uptake to CPF, 2,4-D, and GLY in the common toad R. arenarum induced neurotoxicity, oxidative stress, and immunological depression. Thus, some blood biomarkers employed in our study (B-esterases, GST, levels of TBARS, ACA, and H/L ratio) might be used as predictors in health and ecological risk assessment of amphibian populations exposed to OP insecticides and herbicides.
publishDate	2015
dc.date.none.fl_str_mv	2015-11
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/17631 Lajmanovich, Rafael Carlos; Attademo, Andres Maximiliano; Simoniello, Maria Fernanda; Poletta, Gisela Laura; Junges, Celina Maria; et al.; Harmful Effects of the Dermal Intake of Commercial Formulations Containing Chlorpyrifos, 2,4-D, and Glyphosate on the Common Toad Rhinella arenarum (Anura: Bufonidae); Springer; Water, Air and Soil Pollution; 226; 11-2015; 1-12;427 0049-6979 1573-2932
url	http://hdl.handle.net/11336/17631
identifier_str_mv	Lajmanovich, Rafael Carlos; Attademo, Andres Maximiliano; Simoniello, Maria Fernanda; Poletta, Gisela Laura; Junges, Celina Maria; et al.; Harmful Effects of the Dermal Intake of Commercial Formulations Containing Chlorpyrifos, 2,4-D, and Glyphosate on the Common Toad Rhinella arenarum (Anura: Bufonidae); Springer; Water, Air and Soil Pollution; 226; 11-2015; 1-12;427 0049-6979 1573-2932
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language	spa
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repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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Harmful Effects of the Dermal Intake of Commercial Formulations Containing Chlorpyrifos, 2,4-D, and Glyphosate on the Common Toad Rhinella arenarum (Anura: Bufonidae)

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