Molecular epidemiology of <i>Paenibacillus larvae larvae</i> and incidence of American foulbrood in Argentinean honeys from Buenos Aires province

Autores
Alippi, Adriana Mónica; Reynaldi, Francisco J.; López, Ana C.; De Giusti, Marisa Raquel; Aguilar, Orlando
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Paenibacillus larvae larvae, the causative agent of American foulbrood disease of honey bee larvae occurs throughout the world and is found in all beekeeping areas of Argentina. Microbiological analysis of 394 honey samples obtained from bee hives from Buenos Aires province (Argentina) from three years of sampling (1999–2001) yielded 219 positive cases (55.6%). The incidence of P. l. larvae infected honey samples for 1999 was 68.1% (n = 160), for 2000 47.1% (n = 102), and 46.2% for 2001 (n = 132). The mean values of spore contamination for the three-year study showed a continuous reduction, probably due to good practices of disease management by beekeepers by breeding bees for hygienic behaviour and reduction of antibiotic treatments for control of AFB. P. l. larvae populations isolated from honey were characterized on the basis of DNA fingerprints using the repetitive-sequence-based polymerase chain reaction technique (rep-PCR) with BOX- and REP- sequencespecific primers. Four distinctive patterns, named A, B, C, and D, were distinguishable among the isolates. Genotype D was not observed in previous studies; this finding could be correlated with a new introduction of the disease in Argentina since 1997 when only three genotypes (A, B, and C) were confirmed. The rep-PCR fingerprint patterns obtained were compared with the patterns generated by a world-wide collection of P. l. larvae strains. The same 4 genotypes patterns were found within a collection of strains from 18 different countries of the world. It is important to point out that pattern C was only found in Argentina and in one sample from Uruguay located in the border line, suggesting that genotype C could have been derived from genotype A and disseminated to Uruguay from Argentina. These findings support the hypothesis that American foulbrood disease is exposed to a limited selective pressure from climatic and environmental sources.
Materia
Bioquímica y Biología Molecular
AFB
PCR
DNA fingerprinting
Paenibacillus
Epidemiología Molecular
Apis mellifica
Abejas
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/3857

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network_name_str CIC Digital (CICBA)
spelling Molecular epidemiology of <i>Paenibacillus larvae larvae</i> and incidence of American foulbrood in Argentinean honeys from Buenos Aires provinceAlippi, Adriana MónicaReynaldi, Francisco J.López, Ana C.De Giusti, Marisa RaquelAguilar, OrlandoBioquímica y Biología MolecularAFBPCRDNA fingerprintingPaenibacillusEpidemiología MolecularApis mellificaAbejasPaenibacillus larvae larvae, the causative agent of American foulbrood disease of honey bee larvae occurs throughout the world and is found in all beekeeping areas of Argentina. Microbiological analysis of 394 honey samples obtained from bee hives from Buenos Aires province (Argentina) from three years of sampling (1999–2001) yielded 219 positive cases (55.6%). The incidence of P. l. larvae infected honey samples for 1999 was 68.1% (n = 160), for 2000 47.1% (n = 102), and 46.2% for 2001 (n = 132). The mean values of spore contamination for the three-year study showed a continuous reduction, probably due to good practices of disease management by beekeepers by breeding bees for hygienic behaviour and reduction of antibiotic treatments for control of AFB. P. l. larvae populations isolated from honey were characterized on the basis of DNA fingerprints using the repetitive-sequence-based polymerase chain reaction technique (rep-PCR) with BOX- and REP- sequencespecific primers. Four distinctive patterns, named A, B, C, and D, were distinguishable among the isolates. Genotype D was not observed in previous studies; this finding could be correlated with a new introduction of the disease in Argentina since 1997 when only three genotypes (A, B, and C) were confirmed. The rep-PCR fingerprint patterns obtained were compared with the patterns generated by a world-wide collection of P. l. larvae strains. The same 4 genotypes patterns were found within a collection of strains from 18 different countries of the world. It is important to point out that pattern C was only found in Argentina and in one sample from Uruguay located in the border line, suggesting that genotype C could have been derived from genotype A and disseminated to Uruguay from Argentina. These findings support the hypothesis that American foulbrood disease is exposed to a limited selective pressure from climatic and environmental sources.2004info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/3857enginfo:eu-repo/semantics/altIdentifier/issn/0021-8839Buenos Aires (Argentina)info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:40:08Zoai:digital.cic.gba.gob.ar:11746/3857Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:40:08.417CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Molecular epidemiology of <i>Paenibacillus larvae larvae</i> and incidence of American foulbrood in Argentinean honeys from Buenos Aires province
title Molecular epidemiology of <i>Paenibacillus larvae larvae</i> and incidence of American foulbrood in Argentinean honeys from Buenos Aires province
spellingShingle Molecular epidemiology of <i>Paenibacillus larvae larvae</i> and incidence of American foulbrood in Argentinean honeys from Buenos Aires province
Alippi, Adriana Mónica
Bioquímica y Biología Molecular
AFB
PCR
DNA fingerprinting
Paenibacillus
Epidemiología Molecular
Apis mellifica
Abejas
title_short Molecular epidemiology of <i>Paenibacillus larvae larvae</i> and incidence of American foulbrood in Argentinean honeys from Buenos Aires province
title_full Molecular epidemiology of <i>Paenibacillus larvae larvae</i> and incidence of American foulbrood in Argentinean honeys from Buenos Aires province
title_fullStr Molecular epidemiology of <i>Paenibacillus larvae larvae</i> and incidence of American foulbrood in Argentinean honeys from Buenos Aires province
title_full_unstemmed Molecular epidemiology of <i>Paenibacillus larvae larvae</i> and incidence of American foulbrood in Argentinean honeys from Buenos Aires province
title_sort Molecular epidemiology of <i>Paenibacillus larvae larvae</i> and incidence of American foulbrood in Argentinean honeys from Buenos Aires province
dc.creator.none.fl_str_mv Alippi, Adriana Mónica
Reynaldi, Francisco J.
López, Ana C.
De Giusti, Marisa Raquel
Aguilar, Orlando
author Alippi, Adriana Mónica
author_facet Alippi, Adriana Mónica
Reynaldi, Francisco J.
López, Ana C.
De Giusti, Marisa Raquel
Aguilar, Orlando
author_role author
author2 Reynaldi, Francisco J.
López, Ana C.
De Giusti, Marisa Raquel
Aguilar, Orlando
author2_role author
author
author
author
dc.subject.none.fl_str_mv Bioquímica y Biología Molecular
AFB
PCR
DNA fingerprinting
Paenibacillus
Epidemiología Molecular
Apis mellifica
Abejas
topic Bioquímica y Biología Molecular
AFB
PCR
DNA fingerprinting
Paenibacillus
Epidemiología Molecular
Apis mellifica
Abejas
dc.description.none.fl_txt_mv Paenibacillus larvae larvae, the causative agent of American foulbrood disease of honey bee larvae occurs throughout the world and is found in all beekeeping areas of Argentina. Microbiological analysis of 394 honey samples obtained from bee hives from Buenos Aires province (Argentina) from three years of sampling (1999–2001) yielded 219 positive cases (55.6%). The incidence of P. l. larvae infected honey samples for 1999 was 68.1% (n = 160), for 2000 47.1% (n = 102), and 46.2% for 2001 (n = 132). The mean values of spore contamination for the three-year study showed a continuous reduction, probably due to good practices of disease management by beekeepers by breeding bees for hygienic behaviour and reduction of antibiotic treatments for control of AFB. P. l. larvae populations isolated from honey were characterized on the basis of DNA fingerprints using the repetitive-sequence-based polymerase chain reaction technique (rep-PCR) with BOX- and REP- sequencespecific primers. Four distinctive patterns, named A, B, C, and D, were distinguishable among the isolates. Genotype D was not observed in previous studies; this finding could be correlated with a new introduction of the disease in Argentina since 1997 when only three genotypes (A, B, and C) were confirmed. The rep-PCR fingerprint patterns obtained were compared with the patterns generated by a world-wide collection of P. l. larvae strains. The same 4 genotypes patterns were found within a collection of strains from 18 different countries of the world. It is important to point out that pattern C was only found in Argentina and in one sample from Uruguay located in the border line, suggesting that genotype C could have been derived from genotype A and disseminated to Uruguay from Argentina. These findings support the hypothesis that American foulbrood disease is exposed to a limited selective pressure from climatic and environmental sources.
description Paenibacillus larvae larvae, the causative agent of American foulbrood disease of honey bee larvae occurs throughout the world and is found in all beekeeping areas of Argentina. Microbiological analysis of 394 honey samples obtained from bee hives from Buenos Aires province (Argentina) from three years of sampling (1999–2001) yielded 219 positive cases (55.6%). The incidence of P. l. larvae infected honey samples for 1999 was 68.1% (n = 160), for 2000 47.1% (n = 102), and 46.2% for 2001 (n = 132). The mean values of spore contamination for the three-year study showed a continuous reduction, probably due to good practices of disease management by beekeepers by breeding bees for hygienic behaviour and reduction of antibiotic treatments for control of AFB. P. l. larvae populations isolated from honey were characterized on the basis of DNA fingerprints using the repetitive-sequence-based polymerase chain reaction technique (rep-PCR) with BOX- and REP- sequencespecific primers. Four distinctive patterns, named A, B, C, and D, were distinguishable among the isolates. Genotype D was not observed in previous studies; this finding could be correlated with a new introduction of the disease in Argentina since 1997 when only three genotypes (A, B, and C) were confirmed. The rep-PCR fingerprint patterns obtained were compared with the patterns generated by a world-wide collection of P. l. larvae strains. The same 4 genotypes patterns were found within a collection of strains from 18 different countries of the world. It is important to point out that pattern C was only found in Argentina and in one sample from Uruguay located in the border line, suggesting that genotype C could have been derived from genotype A and disseminated to Uruguay from Argentina. These findings support the hypothesis that American foulbrood disease is exposed to a limited selective pressure from climatic and environmental sources.
publishDate 2004
dc.date.none.fl_str_mv 2004
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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dc.identifier.none.fl_str_mv https://digital.cic.gba.gob.ar/handle/11746/3857
url https://digital.cic.gba.gob.ar/handle/11746/3857
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dc.coverage.none.fl_str_mv Buenos Aires (Argentina)
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instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
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reponame_str CIC Digital (CICBA)
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repository.mail.fl_str_mv marisa.degiusti@sedici.unlp.edu.ar
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