Tetracycline-resistance encoding plasmids from <i>Paenibacillus larvae</i>, the causal agent of American foulbrood disease, isolated from commercial honeys

Autores
Alippi, Adriana Mónica; León, Ignacio Esteban; López, Ana Claudia
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Paenibacillus larvae, the causal agent of American foulbrood disease in honeybees, acquires tetracycline-resistance via native plasmids carrying known tetracycline-resistance determinants. From three P. larvae tetracycline-resistant strains isolated from honeys, 5-kb-circular plasmids with almost identical sequences, designated pPL373 in strain PL373, pPL374 in strain PL374, and pPL395 in strain PL395, were isolated. These plasmids were highly similar (99%) to small tetracycline- encoding plasmids (pMA67, pBHS24, pBSDMV46A, pDMV2, pSU1, pAST4, and pLS55) that replicate by the rolling circle mechanism. Nucleotide sequences comparisons showed that pPL373, pPL374, and pPL395 mainly differed from the previously reported P. larvae plasmid pMA67 in the oriT region and mob genes. These differences suggest alternative mobilization and/or conjugation capacities. Plasmids pPL373, pPL374, and pPL395 were individually transferred by electroporation and stably maintained in tetracycline-susceptible P. larvae NRRL B-14154, in which they autonomously replicated. The presence of nearly identical plasmids in five different genera of gram-positive bacteria, i.e., Bhargavaea, Bacillus, Lactobacillus, Paenibacillus, and Sporosarcina, inhabiting diverse ecological niches provides further evidence of the genetic transfer of tetracycline resistance among environmental bacteria from soils, food, and marine habitats and from pathogenic bacteria such as P. larvae.
Facultad de Ciencias Agrarias y Forestales
Materia
Ciencias Agrarias
Abejas
fitopatología
loque americana
Plásmidos
Tetraciclina
Paenibacillus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/45190

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network_name_str SEDICI (UNLP)
spelling Tetracycline-resistance encoding plasmids from <i>Paenibacillus larvae</i>, the causal agent of American foulbrood disease, isolated from commercial honeysAlippi, Adriana MónicaLeón, Ignacio EstebanLópez, Ana ClaudiaCiencias AgrariasAbejasfitopatologíaloque americanaPlásmidosTetraciclinaPaenibacillusPaenibacillus larvae, the causal agent of American foulbrood disease in honeybees, acquires tetracycline-resistance via native plasmids carrying known tetracycline-resistance determinants. From three P. larvae tetracycline-resistant strains isolated from honeys, 5-kb-circular plasmids with almost identical sequences, designated pPL373 in strain PL373, pPL374 in strain PL374, and pPL395 in strain PL395, were isolated. These plasmids were highly similar (99%) to small tetracycline- encoding plasmids (pMA67, pBHS24, pBSDMV46A, pDMV2, pSU1, pAST4, and pLS55) that replicate by the rolling circle mechanism. Nucleotide sequences comparisons showed that pPL373, pPL374, and pPL395 mainly differed from the previously reported P. larvae plasmid pMA67 in the oriT region and mob genes. These differences suggest alternative mobilization and/or conjugation capacities. Plasmids pPL373, pPL374, and pPL395 were individually transferred by electroporation and stably maintained in tetracycline-susceptible P. larvae NRRL B-14154, in which they autonomously replicated. The presence of nearly identical plasmids in five different genera of gram-positive bacteria, i.e., Bhargavaea, Bacillus, Lactobacillus, Paenibacillus, and Sporosarcina, inhabiting diverse ecological niches provides further evidence of the genetic transfer of tetracycline resistance among environmental bacteria from soils, food, and marine habitats and from pathogenic bacteria such as P. larvae.Facultad de Ciencias Agrarias y Forestales2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf49-61http://sedici.unlp.edu.ar/handle/10915/45190enginfo:eu-repo/semantics/altIdentifier/url/http://revistes.iec.cat/index.php/IM/article/viewFile/85964/85666info:eu-repo/semantics/altIdentifier/issn/1618-1095info:eu-repo/semantics/altIdentifier/doi/10.2436/20.1501.01.207info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Creative Commons Attribution-NonCommercial-NoDerivs 2.5 Argentina (CC BY-NC-ND 2.5)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:34:59Zoai:sedici.unlp.edu.ar:10915/45190Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:35:00.115SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Tetracycline-resistance encoding plasmids from <i>Paenibacillus larvae</i>, the causal agent of American foulbrood disease, isolated from commercial honeys
title Tetracycline-resistance encoding plasmids from <i>Paenibacillus larvae</i>, the causal agent of American foulbrood disease, isolated from commercial honeys
spellingShingle Tetracycline-resistance encoding plasmids from <i>Paenibacillus larvae</i>, the causal agent of American foulbrood disease, isolated from commercial honeys
Alippi, Adriana Mónica
Ciencias Agrarias
Abejas
fitopatología
loque americana
Plásmidos
Tetraciclina
Paenibacillus
title_short Tetracycline-resistance encoding plasmids from <i>Paenibacillus larvae</i>, the causal agent of American foulbrood disease, isolated from commercial honeys
title_full Tetracycline-resistance encoding plasmids from <i>Paenibacillus larvae</i>, the causal agent of American foulbrood disease, isolated from commercial honeys
title_fullStr Tetracycline-resistance encoding plasmids from <i>Paenibacillus larvae</i>, the causal agent of American foulbrood disease, isolated from commercial honeys
title_full_unstemmed Tetracycline-resistance encoding plasmids from <i>Paenibacillus larvae</i>, the causal agent of American foulbrood disease, isolated from commercial honeys
title_sort Tetracycline-resistance encoding plasmids from <i>Paenibacillus larvae</i>, the causal agent of American foulbrood disease, isolated from commercial honeys
dc.creator.none.fl_str_mv Alippi, Adriana Mónica
León, Ignacio Esteban
López, Ana Claudia
author Alippi, Adriana Mónica
author_facet Alippi, Adriana Mónica
León, Ignacio Esteban
López, Ana Claudia
author_role author
author2 León, Ignacio Esteban
López, Ana Claudia
author2_role author
author
dc.subject.none.fl_str_mv Ciencias Agrarias
Abejas
fitopatología
loque americana
Plásmidos
Tetraciclina
Paenibacillus
topic Ciencias Agrarias
Abejas
fitopatología
loque americana
Plásmidos
Tetraciclina
Paenibacillus
dc.description.none.fl_txt_mv Paenibacillus larvae, the causal agent of American foulbrood disease in honeybees, acquires tetracycline-resistance via native plasmids carrying known tetracycline-resistance determinants. From three P. larvae tetracycline-resistant strains isolated from honeys, 5-kb-circular plasmids with almost identical sequences, designated pPL373 in strain PL373, pPL374 in strain PL374, and pPL395 in strain PL395, were isolated. These plasmids were highly similar (99%) to small tetracycline- encoding plasmids (pMA67, pBHS24, pBSDMV46A, pDMV2, pSU1, pAST4, and pLS55) that replicate by the rolling circle mechanism. Nucleotide sequences comparisons showed that pPL373, pPL374, and pPL395 mainly differed from the previously reported P. larvae plasmid pMA67 in the oriT region and mob genes. These differences suggest alternative mobilization and/or conjugation capacities. Plasmids pPL373, pPL374, and pPL395 were individually transferred by electroporation and stably maintained in tetracycline-susceptible P. larvae NRRL B-14154, in which they autonomously replicated. The presence of nearly identical plasmids in five different genera of gram-positive bacteria, i.e., Bhargavaea, Bacillus, Lactobacillus, Paenibacillus, and Sporosarcina, inhabiting diverse ecological niches provides further evidence of the genetic transfer of tetracycline resistance among environmental bacteria from soils, food, and marine habitats and from pathogenic bacteria such as P. larvae.
Facultad de Ciencias Agrarias y Forestales
description Paenibacillus larvae, the causal agent of American foulbrood disease in honeybees, acquires tetracycline-resistance via native plasmids carrying known tetracycline-resistance determinants. From three P. larvae tetracycline-resistant strains isolated from honeys, 5-kb-circular plasmids with almost identical sequences, designated pPL373 in strain PL373, pPL374 in strain PL374, and pPL395 in strain PL395, were isolated. These plasmids were highly similar (99%) to small tetracycline- encoding plasmids (pMA67, pBHS24, pBSDMV46A, pDMV2, pSU1, pAST4, and pLS55) that replicate by the rolling circle mechanism. Nucleotide sequences comparisons showed that pPL373, pPL374, and pPL395 mainly differed from the previously reported P. larvae plasmid pMA67 in the oriT region and mob genes. These differences suggest alternative mobilization and/or conjugation capacities. Plasmids pPL373, pPL374, and pPL395 were individually transferred by electroporation and stably maintained in tetracycline-susceptible P. larvae NRRL B-14154, in which they autonomously replicated. The presence of nearly identical plasmids in five different genera of gram-positive bacteria, i.e., Bhargavaea, Bacillus, Lactobacillus, Paenibacillus, and Sporosarcina, inhabiting diverse ecological niches provides further evidence of the genetic transfer of tetracycline resistance among environmental bacteria from soils, food, and marine habitats and from pathogenic bacteria such as P. larvae.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/45190
url http://sedici.unlp.edu.ar/handle/10915/45190
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://revistes.iec.cat/index.php/IM/article/viewFile/85964/85666
info:eu-repo/semantics/altIdentifier/issn/1618-1095
info:eu-repo/semantics/altIdentifier/doi/10.2436/20.1501.01.207
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Creative Commons Attribution-NonCommercial-NoDerivs 2.5 Argentina (CC BY-NC-ND 2.5)
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Creative Commons Attribution-NonCommercial-NoDerivs 2.5 Argentina (CC BY-NC-ND 2.5)
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