Validation of a Simple Molecular Method for Assessment of Clonal Heterogeneity in Kiwifruit (Actinidia deliciosa A. Chev.)
- Autores
- Briguglio, Mauro Andrés; Ispizúa, Verónica; Godoy, Carlos; Marcellán, Olga
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión enviada
- Descripción
- Aims:The industry of the green fleshed kiwifruit over the world is based on the cultivar Hayward due to outstanding characteristics of its fruits. Mutations and improper labeling of introduced plants could give rise to clonal heterogeneity within kiwifruit cultivars. Therefore, the objective is to validate a simple molecular approach to evaluate the clonal purity of cultivar Hayward in micropropagation laboratories and nurseries. Study Design:Molecular analysis of the genetic variability within cultivar. Place and Duration of Study:Biotechnology Laboratory at the Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata – Estación Experimental Agropecuaria Balcarce, Instituto Nacional de Tecnología Agropecuaria, Argentina, 2013. Methodology:Genomic DNA of four introductions and clone 8 of cultivar Hayward was amplified with randomly amplified polymorphic DNA markers. Cluster and principal coordinates analyses were performed. Results:The analyses revealed that all introductions were genetically different from each other. Conclusion:Small genetic differences within cultivars could be detected by this simple molecular approach when it is performed under well-controlled experimental conditions in each micropropagation laboratory or nursery.
- Materia
-
Agronomía, reproducción y protección de plantas
Cultivar Hayward
genetic variability
RAPD markers
cluster and principal coordinates analyses - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
- OAI Identificador
- oai:digital.cic.gba.gob.ar:11746/5409
Ver los metadatos del registro completo
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Validation of a Simple Molecular Method for Assessment of Clonal Heterogeneity in Kiwifruit (Actinidia deliciosa A. Chev.)Briguglio, Mauro AndrésIspizúa, VerónicaGodoy, CarlosMarcellán, OlgaAgronomía, reproducción y protección de plantasCultivar Haywardgenetic variabilityRAPD markerscluster and principal coordinates analyses<strong>Aims:</strong>The industry of the green fleshed kiwifruit over the world is based on the cultivar Hayward due to outstanding characteristics of its fruits. Mutations and improper labeling of introduced plants could give rise to clonal heterogeneity within kiwifruit cultivars. Therefore, the objective is to validate a simple molecular approach to evaluate the clonal purity of cultivar Hayward in micropropagation laboratories and nurseries. <strong>Study Design:</strong>Molecular analysis of the genetic variability within cultivar. <strong>Place and Duration of Study:</strong>Biotechnology Laboratory at the Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata – Estación Experimental Agropecuaria Balcarce, Instituto Nacional de Tecnología Agropecuaria, Argentina, 2013. <strong>Methodology:</strong>Genomic DNA of four introductions and clone 8 of cultivar Hayward was amplified with randomly amplified polymorphic DNA markers. Cluster and principal coordinates analyses were performed. <strong>Results:</strong>The analyses revealed that all introductions were genetically different from each other. <strong>Conclusion:</strong>Small genetic differences within cultivars could be detected by this simple molecular approach when it is performed under well-controlled experimental conditions in each micropropagation laboratory or nursery.2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/5409enghttp://digital.cic.gba.gob.ar/handle/11746/5408info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:39:51Zoai:digital.cic.gba.gob.ar:11746/5409Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:39:52.103CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse |
dc.title.none.fl_str_mv |
Validation of a Simple Molecular Method for Assessment of Clonal Heterogeneity in Kiwifruit (Actinidia deliciosa A. Chev.) |
title |
Validation of a Simple Molecular Method for Assessment of Clonal Heterogeneity in Kiwifruit (Actinidia deliciosa A. Chev.) |
spellingShingle |
Validation of a Simple Molecular Method for Assessment of Clonal Heterogeneity in Kiwifruit (Actinidia deliciosa A. Chev.) Briguglio, Mauro Andrés Agronomía, reproducción y protección de plantas Cultivar Hayward genetic variability RAPD markers cluster and principal coordinates analyses |
title_short |
Validation of a Simple Molecular Method for Assessment of Clonal Heterogeneity in Kiwifruit (Actinidia deliciosa A. Chev.) |
title_full |
Validation of a Simple Molecular Method for Assessment of Clonal Heterogeneity in Kiwifruit (Actinidia deliciosa A. Chev.) |
title_fullStr |
Validation of a Simple Molecular Method for Assessment of Clonal Heterogeneity in Kiwifruit (Actinidia deliciosa A. Chev.) |
title_full_unstemmed |
Validation of a Simple Molecular Method for Assessment of Clonal Heterogeneity in Kiwifruit (Actinidia deliciosa A. Chev.) |
title_sort |
Validation of a Simple Molecular Method for Assessment of Clonal Heterogeneity in Kiwifruit (Actinidia deliciosa A. Chev.) |
dc.creator.none.fl_str_mv |
Briguglio, Mauro Andrés Ispizúa, Verónica Godoy, Carlos Marcellán, Olga |
author |
Briguglio, Mauro Andrés |
author_facet |
Briguglio, Mauro Andrés Ispizúa, Verónica Godoy, Carlos Marcellán, Olga |
author_role |
author |
author2 |
Ispizúa, Verónica Godoy, Carlos Marcellán, Olga |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Agronomía, reproducción y protección de plantas Cultivar Hayward genetic variability RAPD markers cluster and principal coordinates analyses |
topic |
Agronomía, reproducción y protección de plantas Cultivar Hayward genetic variability RAPD markers cluster and principal coordinates analyses |
dc.description.none.fl_txt_mv |
<strong>Aims:</strong>The industry of the green fleshed kiwifruit over the world is based on the cultivar Hayward due to outstanding characteristics of its fruits. Mutations and improper labeling of introduced plants could give rise to clonal heterogeneity within kiwifruit cultivars. Therefore, the objective is to validate a simple molecular approach to evaluate the clonal purity of cultivar Hayward in micropropagation laboratories and nurseries. <strong>Study Design:</strong>Molecular analysis of the genetic variability within cultivar. <strong>Place and Duration of Study:</strong>Biotechnology Laboratory at the Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata – Estación Experimental Agropecuaria Balcarce, Instituto Nacional de Tecnología Agropecuaria, Argentina, 2013. <strong>Methodology:</strong>Genomic DNA of four introductions and clone 8 of cultivar Hayward was amplified with randomly amplified polymorphic DNA markers. Cluster and principal coordinates analyses were performed. <strong>Results:</strong>The analyses revealed that all introductions were genetically different from each other. <strong>Conclusion:</strong>Small genetic differences within cultivars could be detected by this simple molecular approach when it is performed under well-controlled experimental conditions in each micropropagation laboratory or nursery. |
description |
<strong>Aims:</strong>The industry of the green fleshed kiwifruit over the world is based on the cultivar Hayward due to outstanding characteristics of its fruits. Mutations and improper labeling of introduced plants could give rise to clonal heterogeneity within kiwifruit cultivars. Therefore, the objective is to validate a simple molecular approach to evaluate the clonal purity of cultivar Hayward in micropropagation laboratories and nurseries. <strong>Study Design:</strong>Molecular analysis of the genetic variability within cultivar. <strong>Place and Duration of Study:</strong>Biotechnology Laboratory at the Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata – Estación Experimental Agropecuaria Balcarce, Instituto Nacional de Tecnología Agropecuaria, Argentina, 2013. <strong>Methodology:</strong>Genomic DNA of four introductions and clone 8 of cultivar Hayward was amplified with randomly amplified polymorphic DNA markers. Cluster and principal coordinates analyses were performed. <strong>Results:</strong>The analyses revealed that all introductions were genetically different from each other. <strong>Conclusion:</strong>Small genetic differences within cultivars could be detected by this simple molecular approach when it is performed under well-controlled experimental conditions in each micropropagation laboratory or nursery. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/submittedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
submittedVersion |
dc.identifier.none.fl_str_mv |
https://digital.cic.gba.gob.ar/handle/11746/5409 |
url |
https://digital.cic.gba.gob.ar/handle/11746/5409 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://digital.cic.gba.gob.ar/handle/11746/5408 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:CIC Digital (CICBA) instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires instacron:CICBA |
reponame_str |
CIC Digital (CICBA) |
collection |
CIC Digital (CICBA) |
instname_str |
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
instacron_str |
CICBA |
institution |
CICBA |
repository.name.fl_str_mv |
CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
repository.mail.fl_str_mv |
marisa.degiusti@sedici.unlp.edu.ar |
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13.070432 |