Validation of a Simple Molecular Method for Assessment of Clonal Heterogeneity in Kiwifruit (Actinidia deliciosa A. Chev.)

Autores
Briguglio, Mauro Andrés; Ispizúa, Verónica; Godoy, Carlos; Marcellán, Olga
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión enviada
Descripción
Aims:The industry of the green fleshed kiwifruit over the world is based on the cultivar Hayward due to outstanding characteristics of its fruits. Mutations and improper labeling of introduced plants could give rise to clonal heterogeneity within kiwifruit cultivars. Therefore, the objective is to validate a simple molecular approach to evaluate the clonal purity of cultivar Hayward in micropropagation laboratories and nurseries. Study Design:Molecular analysis of the genetic variability within cultivar. Place and Duration of Study:Biotechnology Laboratory at the Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata – Estación Experimental Agropecuaria Balcarce, Instituto Nacional de Tecnología Agropecuaria, Argentina, 2013. Methodology:Genomic DNA of four introductions and clone 8 of cultivar Hayward was amplified with randomly amplified polymorphic DNA markers. Cluster and principal coordinates analyses were performed. Results:The analyses revealed that all introductions were genetically different from each other. Conclusion:Small genetic differences within cultivars could be detected by this simple molecular approach when it is performed under well-controlled experimental conditions in each micropropagation laboratory or nursery.
Materia
Agronomía, reproducción y protección de plantas
Cultivar Hayward
genetic variability
RAPD markers
cluster and principal coordinates analyses
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/5409

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oai_identifier_str oai:digital.cic.gba.gob.ar:11746/5409
network_acronym_str CICBA
repository_id_str 9441
network_name_str CIC Digital (CICBA)
spelling Validation of a Simple Molecular Method for Assessment of Clonal Heterogeneity in Kiwifruit (Actinidia deliciosa A. Chev.)Briguglio, Mauro AndrésIspizúa, VerónicaGodoy, CarlosMarcellán, OlgaAgronomía, reproducción y protección de plantasCultivar Haywardgenetic variabilityRAPD markerscluster and principal coordinates analyses<strong>Aims:</strong>The industry of the green fleshed kiwifruit over the world is based on the cultivar Hayward due to outstanding characteristics of its fruits. Mutations and improper labeling of introduced plants could give rise to clonal heterogeneity within kiwifruit cultivars. Therefore, the objective is to validate a simple molecular approach to evaluate the clonal purity of cultivar Hayward in micropropagation laboratories and nurseries. <strong>Study Design:</strong>Molecular analysis of the genetic variability within cultivar. <strong>Place and Duration of Study:</strong>Biotechnology Laboratory at the Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata – Estación Experimental Agropecuaria Balcarce, Instituto Nacional de Tecnología Agropecuaria, Argentina, 2013. <strong>Methodology:</strong>Genomic DNA of four introductions and clone 8 of cultivar Hayward was amplified with randomly amplified polymorphic DNA markers. Cluster and principal coordinates analyses were performed. <strong>Results:</strong>The analyses revealed that all introductions were genetically different from each other. <strong>Conclusion:</strong>Small genetic differences within cultivars could be detected by this simple molecular approach when it is performed under well-controlled experimental conditions in each micropropagation laboratory or nursery.2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/5409enghttp://digital.cic.gba.gob.ar/handle/11746/5408info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:39:51Zoai:digital.cic.gba.gob.ar:11746/5409Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:39:52.103CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Validation of a Simple Molecular Method for Assessment of Clonal Heterogeneity in Kiwifruit (Actinidia deliciosa A. Chev.)
title Validation of a Simple Molecular Method for Assessment of Clonal Heterogeneity in Kiwifruit (Actinidia deliciosa A. Chev.)
spellingShingle Validation of a Simple Molecular Method for Assessment of Clonal Heterogeneity in Kiwifruit (Actinidia deliciosa A. Chev.)
Briguglio, Mauro Andrés
Agronomía, reproducción y protección de plantas
Cultivar Hayward
genetic variability
RAPD markers
cluster and principal coordinates analyses
title_short Validation of a Simple Molecular Method for Assessment of Clonal Heterogeneity in Kiwifruit (Actinidia deliciosa A. Chev.)
title_full Validation of a Simple Molecular Method for Assessment of Clonal Heterogeneity in Kiwifruit (Actinidia deliciosa A. Chev.)
title_fullStr Validation of a Simple Molecular Method for Assessment of Clonal Heterogeneity in Kiwifruit (Actinidia deliciosa A. Chev.)
title_full_unstemmed Validation of a Simple Molecular Method for Assessment of Clonal Heterogeneity in Kiwifruit (Actinidia deliciosa A. Chev.)
title_sort Validation of a Simple Molecular Method for Assessment of Clonal Heterogeneity in Kiwifruit (Actinidia deliciosa A. Chev.)
dc.creator.none.fl_str_mv Briguglio, Mauro Andrés
Ispizúa, Verónica
Godoy, Carlos
Marcellán, Olga
author Briguglio, Mauro Andrés
author_facet Briguglio, Mauro Andrés
Ispizúa, Verónica
Godoy, Carlos
Marcellán, Olga
author_role author
author2 Ispizúa, Verónica
Godoy, Carlos
Marcellán, Olga
author2_role author
author
author
dc.subject.none.fl_str_mv Agronomía, reproducción y protección de plantas
Cultivar Hayward
genetic variability
RAPD markers
cluster and principal coordinates analyses
topic Agronomía, reproducción y protección de plantas
Cultivar Hayward
genetic variability
RAPD markers
cluster and principal coordinates analyses
dc.description.none.fl_txt_mv <strong>Aims:</strong>The industry of the green fleshed kiwifruit over the world is based on the cultivar Hayward due to outstanding characteristics of its fruits. Mutations and improper labeling of introduced plants could give rise to clonal heterogeneity within kiwifruit cultivars. Therefore, the objective is to validate a simple molecular approach to evaluate the clonal purity of cultivar Hayward in micropropagation laboratories and nurseries. <strong>Study Design:</strong>Molecular analysis of the genetic variability within cultivar. <strong>Place and Duration of Study:</strong>Biotechnology Laboratory at the Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata – Estación Experimental Agropecuaria Balcarce, Instituto Nacional de Tecnología Agropecuaria, Argentina, 2013. <strong>Methodology:</strong>Genomic DNA of four introductions and clone 8 of cultivar Hayward was amplified with randomly amplified polymorphic DNA markers. Cluster and principal coordinates analyses were performed. <strong>Results:</strong>The analyses revealed that all introductions were genetically different from each other. <strong>Conclusion:</strong>Small genetic differences within cultivars could be detected by this simple molecular approach when it is performed under well-controlled experimental conditions in each micropropagation laboratory or nursery.
description <strong>Aims:</strong>The industry of the green fleshed kiwifruit over the world is based on the cultivar Hayward due to outstanding characteristics of its fruits. Mutations and improper labeling of introduced plants could give rise to clonal heterogeneity within kiwifruit cultivars. Therefore, the objective is to validate a simple molecular approach to evaluate the clonal purity of cultivar Hayward in micropropagation laboratories and nurseries. <strong>Study Design:</strong>Molecular analysis of the genetic variability within cultivar. <strong>Place and Duration of Study:</strong>Biotechnology Laboratory at the Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata – Estación Experimental Agropecuaria Balcarce, Instituto Nacional de Tecnología Agropecuaria, Argentina, 2013. <strong>Methodology:</strong>Genomic DNA of four introductions and clone 8 of cultivar Hayward was amplified with randomly amplified polymorphic DNA markers. Cluster and principal coordinates analyses were performed. <strong>Results:</strong>The analyses revealed that all introductions were genetically different from each other. <strong>Conclusion:</strong>Small genetic differences within cultivars could be detected by this simple molecular approach when it is performed under well-controlled experimental conditions in each micropropagation laboratory or nursery.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv https://digital.cic.gba.gob.ar/handle/11746/5409
url https://digital.cic.gba.gob.ar/handle/11746/5409
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://digital.cic.gba.gob.ar/handle/11746/5408
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:CIC Digital (CICBA)
instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron:CICBA
reponame_str CIC Digital (CICBA)
collection CIC Digital (CICBA)
instname_str Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron_str CICBA
institution CICBA
repository.name.fl_str_mv CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
repository.mail.fl_str_mv marisa.degiusti@sedici.unlp.edu.ar
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