Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cells
- Autores
- Mercer, Natalia; Ahmed, Hafiz; Etcheverry, Susana B.; Vasta, Gerardo R.; Cortizo, Ana María
- Año de publicación
- 2007
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión enviada
- Descripción
- Advanced glycation end products (AGEs) have been proposed as the pathological mechanisms underlying diabetic chronic complications. They may also play a role in the pathogenesis of diabetic osteopenia, although their mechanisms of action remain unclear. We investigated the protein (immunofluorescence) and gene expression (realtime RT-PCR) of two receptors for AGEs, RAGE and galectin-3, as well as their regulation by AGEs, and the apoptotic effect on osteoblast-like cells (UMR106 and MC3T3E1) in culture. AGEs up-regulated the expression of RAGE and galectin-3 in both cells lines. These effects were accompanied by an increase in the corresponding mRNA in the non-tumoral MC3T3E1 but not in the osteosarcoma UMR106 cells. Finally, we demonstrated that a 24 h exposure to AGEs induced apoptosis in both cell lines. Thus, AGEs-receptors may play important roles in the bone alterations described in aging and diabetic patients.
- Materia
-
Ciencias Químicas
Advanced glycation end products
RAGE
Galectin-3
Osteoblasts
Apoptosis
AGE-receptors
Regulation - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
- OAI Identificador
- oai:digital.cic.gba.gob.ar:11746/4960
Ver los metadatos del registro completo
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Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cellsMercer, NataliaAhmed, HafizEtcheverry, Susana B.Vasta, Gerardo R.Cortizo, Ana MaríaCiencias QuímicasAdvanced glycation end productsRAGEGalectin-3OsteoblastsApoptosisAGE-receptorsRegulationAdvanced glycation end products (AGEs) have been proposed as the pathological mechanisms underlying diabetic chronic complications. They may also play a role in the pathogenesis of diabetic osteopenia, although their mechanisms of action remain unclear. We investigated the protein (immunofluorescence) and gene expression (realtime RT-PCR) of two receptors for AGEs, RAGE and galectin-3, as well as their regulation by AGEs, and the apoptotic effect on osteoblast-like cells (UMR106 and MC3T3E1) in culture. AGEs up-regulated the expression of RAGE and galectin-3 in both cells lines. These effects were accompanied by an increase in the corresponding mRNA in the non-tumoral MC3T3E1 but not in the osteosarcoma UMR106 cells. Finally, we demonstrated that a 24 h exposure to AGEs induced apoptosis in both cell lines. Thus, AGEs-receptors may play important roles in the bone alterations described in aging and diabetic patients.2007info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/4960enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:39:51Zoai:digital.cic.gba.gob.ar:11746/4960Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:39:51.341CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse |
dc.title.none.fl_str_mv |
Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cells |
title |
Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cells |
spellingShingle |
Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cells Mercer, Natalia Ciencias Químicas Advanced glycation end products RAGE Galectin-3 Osteoblasts Apoptosis AGE-receptors Regulation |
title_short |
Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cells |
title_full |
Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cells |
title_fullStr |
Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cells |
title_full_unstemmed |
Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cells |
title_sort |
Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cells |
dc.creator.none.fl_str_mv |
Mercer, Natalia Ahmed, Hafiz Etcheverry, Susana B. Vasta, Gerardo R. Cortizo, Ana María |
author |
Mercer, Natalia |
author_facet |
Mercer, Natalia Ahmed, Hafiz Etcheverry, Susana B. Vasta, Gerardo R. Cortizo, Ana María |
author_role |
author |
author2 |
Ahmed, Hafiz Etcheverry, Susana B. Vasta, Gerardo R. Cortizo, Ana María |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Ciencias Químicas Advanced glycation end products RAGE Galectin-3 Osteoblasts Apoptosis AGE-receptors Regulation |
topic |
Ciencias Químicas Advanced glycation end products RAGE Galectin-3 Osteoblasts Apoptosis AGE-receptors Regulation |
dc.description.none.fl_txt_mv |
Advanced glycation end products (AGEs) have been proposed as the pathological mechanisms underlying diabetic chronic complications. They may also play a role in the pathogenesis of diabetic osteopenia, although their mechanisms of action remain unclear. We investigated the protein (immunofluorescence) and gene expression (realtime RT-PCR) of two receptors for AGEs, RAGE and galectin-3, as well as their regulation by AGEs, and the apoptotic effect on osteoblast-like cells (UMR106 and MC3T3E1) in culture. AGEs up-regulated the expression of RAGE and galectin-3 in both cells lines. These effects were accompanied by an increase in the corresponding mRNA in the non-tumoral MC3T3E1 but not in the osteosarcoma UMR106 cells. Finally, we demonstrated that a 24 h exposure to AGEs induced apoptosis in both cell lines. Thus, AGEs-receptors may play important roles in the bone alterations described in aging and diabetic patients. |
description |
Advanced glycation end products (AGEs) have been proposed as the pathological mechanisms underlying diabetic chronic complications. They may also play a role in the pathogenesis of diabetic osteopenia, although their mechanisms of action remain unclear. We investigated the protein (immunofluorescence) and gene expression (realtime RT-PCR) of two receptors for AGEs, RAGE and galectin-3, as well as their regulation by AGEs, and the apoptotic effect on osteoblast-like cells (UMR106 and MC3T3E1) in culture. AGEs up-regulated the expression of RAGE and galectin-3 in both cells lines. These effects were accompanied by an increase in the corresponding mRNA in the non-tumoral MC3T3E1 but not in the osteosarcoma UMR106 cells. Finally, we demonstrated that a 24 h exposure to AGEs induced apoptosis in both cell lines. Thus, AGEs-receptors may play important roles in the bone alterations described in aging and diabetic patients. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/conferenceObject info:eu-repo/semantics/submittedVersion http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
format |
conferenceObject |
status_str |
submittedVersion |
dc.identifier.none.fl_str_mv |
https://digital.cic.gba.gob.ar/handle/11746/4960 |
url |
https://digital.cic.gba.gob.ar/handle/11746/4960 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:CIC Digital (CICBA) instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires instacron:CICBA |
reponame_str |
CIC Digital (CICBA) |
collection |
CIC Digital (CICBA) |
instname_str |
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
instacron_str |
CICBA |
institution |
CICBA |
repository.name.fl_str_mv |
CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
repository.mail.fl_str_mv |
marisa.degiusti@sedici.unlp.edu.ar |
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1844618580816035840 |
score |
13.070432 |