Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cells

Autores
Mercer, Natalia; Ahmed, Hafiz; Etcheverry, Susana B.; Vasta, Gerardo R.; Cortizo, Ana María
Año de publicación
2007
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión enviada
Descripción
Advanced glycation end products (AGEs) have been proposed as the pathological mechanisms underlying diabetic chronic complications. They may also play a role in the pathogenesis of diabetic osteopenia, although their mechanisms of action remain unclear. We investigated the protein (immunofluorescence) and gene expression (realtime RT-PCR) of two receptors for AGEs, RAGE and galectin-3, as well as their regulation by AGEs, and the apoptotic effect on osteoblast-like cells (UMR106 and MC3T3E1) in culture. AGEs up-regulated the expression of RAGE and galectin-3 in both cells lines. These effects were accompanied by an increase in the corresponding mRNA in the non-tumoral MC3T3E1 but not in the osteosarcoma UMR106 cells. Finally, we demonstrated that a 24 h exposure to AGEs induced apoptosis in both cell lines. Thus, AGEs-receptors may play important roles in the bone alterations described in aging and diabetic patients.
Materia
Ciencias Químicas
Advanced glycation end products
RAGE
Galectin-3
Osteoblasts
Apoptosis
AGE-receptors
Regulation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/4960

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network_name_str CIC Digital (CICBA)
spelling Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cellsMercer, NataliaAhmed, HafizEtcheverry, Susana B.Vasta, Gerardo R.Cortizo, Ana MaríaCiencias QuímicasAdvanced glycation end productsRAGEGalectin-3OsteoblastsApoptosisAGE-receptorsRegulationAdvanced glycation end products (AGEs) have been proposed as the pathological mechanisms underlying diabetic chronic complications. They may also play a role in the pathogenesis of diabetic osteopenia, although their mechanisms of action remain unclear. We investigated the protein (immunofluorescence) and gene expression (realtime RT-PCR) of two receptors for AGEs, RAGE and galectin-3, as well as their regulation by AGEs, and the apoptotic effect on osteoblast-like cells (UMR106 and MC3T3E1) in culture. AGEs up-regulated the expression of RAGE and galectin-3 in both cells lines. These effects were accompanied by an increase in the corresponding mRNA in the non-tumoral MC3T3E1 but not in the osteosarcoma UMR106 cells. Finally, we demonstrated that a 24 h exposure to AGEs induced apoptosis in both cell lines. Thus, AGEs-receptors may play important roles in the bone alterations described in aging and diabetic patients.2007info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/4960enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:39:51Zoai:digital.cic.gba.gob.ar:11746/4960Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:39:51.341CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cells
title Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cells
spellingShingle Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cells
Mercer, Natalia
Ciencias Químicas
Advanced glycation end products
RAGE
Galectin-3
Osteoblasts
Apoptosis
AGE-receptors
Regulation
title_short Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cells
title_full Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cells
title_fullStr Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cells
title_full_unstemmed Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cells
title_sort Regulation of advanced glycation end product (AGE) receptors and apoptosis by AGEs in osteoblast-like cells
dc.creator.none.fl_str_mv Mercer, Natalia
Ahmed, Hafiz
Etcheverry, Susana B.
Vasta, Gerardo R.
Cortizo, Ana María
author Mercer, Natalia
author_facet Mercer, Natalia
Ahmed, Hafiz
Etcheverry, Susana B.
Vasta, Gerardo R.
Cortizo, Ana María
author_role author
author2 Ahmed, Hafiz
Etcheverry, Susana B.
Vasta, Gerardo R.
Cortizo, Ana María
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Químicas
Advanced glycation end products
RAGE
Galectin-3
Osteoblasts
Apoptosis
AGE-receptors
Regulation
topic Ciencias Químicas
Advanced glycation end products
RAGE
Galectin-3
Osteoblasts
Apoptosis
AGE-receptors
Regulation
dc.description.none.fl_txt_mv Advanced glycation end products (AGEs) have been proposed as the pathological mechanisms underlying diabetic chronic complications. They may also play a role in the pathogenesis of diabetic osteopenia, although their mechanisms of action remain unclear. We investigated the protein (immunofluorescence) and gene expression (realtime RT-PCR) of two receptors for AGEs, RAGE and galectin-3, as well as their regulation by AGEs, and the apoptotic effect on osteoblast-like cells (UMR106 and MC3T3E1) in culture. AGEs up-regulated the expression of RAGE and galectin-3 in both cells lines. These effects were accompanied by an increase in the corresponding mRNA in the non-tumoral MC3T3E1 but not in the osteosarcoma UMR106 cells. Finally, we demonstrated that a 24 h exposure to AGEs induced apoptosis in both cell lines. Thus, AGEs-receptors may play important roles in the bone alterations described in aging and diabetic patients.
description Advanced glycation end products (AGEs) have been proposed as the pathological mechanisms underlying diabetic chronic complications. They may also play a role in the pathogenesis of diabetic osteopenia, although their mechanisms of action remain unclear. We investigated the protein (immunofluorescence) and gene expression (realtime RT-PCR) of two receptors for AGEs, RAGE and galectin-3, as well as their regulation by AGEs, and the apoptotic effect on osteoblast-like cells (UMR106 and MC3T3E1) in culture. AGEs up-regulated the expression of RAGE and galectin-3 in both cells lines. These effects were accompanied by an increase in the corresponding mRNA in the non-tumoral MC3T3E1 but not in the osteosarcoma UMR106 cells. Finally, we demonstrated that a 24 h exposure to AGEs induced apoptosis in both cell lines. Thus, AGEs-receptors may play important roles in the bone alterations described in aging and diabetic patients.
publishDate 2007
dc.date.none.fl_str_mv 2007
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/submittedVersion
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
format conferenceObject
status_str submittedVersion
dc.identifier.none.fl_str_mv https://digital.cic.gba.gob.ar/handle/11746/4960
url https://digital.cic.gba.gob.ar/handle/11746/4960
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:CIC Digital (CICBA)
instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron:CICBA
reponame_str CIC Digital (CICBA)
collection CIC Digital (CICBA)
instname_str Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron_str CICBA
institution CICBA
repository.name.fl_str_mv CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
repository.mail.fl_str_mv marisa.degiusti@sedici.unlp.edu.ar
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