Degradation of chlorophenol mixtures in a fed-­batch system by two soil bacteria

Autores
Durruty, Ignacio; Okada, Elena; González, Jorge Froilán; Murialdo, Silvia Elena
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This work was undertaken to investígate the effect of variations of the feed rate on a fed-batch set-up used to degrade xenobiotics. The mixture of substrates was composed of PCP, 2,4,6 TCP and 2,3,5,6 TeCP (pentachlorophenol, 2,4,6 trichlo- rophenol and 2,3,5,6 tetrachlorophenol respectively). Two acclimated bacteria isolated from soil were used: Pseudomonas aeruginosa and Achromobacter sp. nov. The different flow rates tested were: I: 0.5 ml*min-1, II: 1.67 ml*min$latex ^{} $-1 and III: 2.00 mf-min'1. Our results show that during fed-batch operation the 2,4,6 TCP exhibits an earlier degradation than the other com- pounds, for all of the flow rates tested. This indicates that in this case the degradation of the most recalcitrant compounds (PCP and 2,3,5,6 TeCP) is benefited by the increase in biomass ofbacteria, due to the metabolisation of a less recalcitrant compound (2,4,6 TCP). The defined parameter, specific degradation rate (SDR), was demonstrated to be very useful for comparing the degradation abilities at different flow rates of a fed-batch system. The degradation efficiencies were shown to be higher than 90% for all of the cases and to decrease as the feed rate increases. However, the SDR, a parameter that involves the rate of degradation and the biomass, increases as the flow rate increases. At a feed flow rate of 2 mf-min'1 SDR reaches a maximum of 12.476 x 10'10 mgCP-M-CFU'1. Finally, among the feed flows tested, taking into account both the degradation efficiency and the SDR, 2 ml-min-1 is the most convenient flow rate for chlorophenol degradation in fed-batch Systems. An even higher degradation efficiency (97%) can be achieved by using the feed rate of 2 mf-min'1 followed by an additional batch post-treatment of2 h, with a SDR of 13.136 x 10-10 mg CP-h1-CFU1.
Materia
Bioquímica y Biología Molecular
Clorofenoles
Biodegradación Ambiental
Biocatálisis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/4078

id CICBA_c019024853fb1956287d5debc13cae31
oai_identifier_str oai:digital.cic.gba.gob.ar:11746/4078
network_acronym_str CICBA
repository_id_str 9441
network_name_str CIC Digital (CICBA)
spelling Degradation of chlorophenol mixtures in a fed-­batch system by two soil bacteriaDurruty, IgnacioOkada, ElenaGonzález, Jorge FroilánMurialdo, Silvia ElenaBioquímica y Biología MolecularClorofenolesBiodegradación AmbientalBiocatálisisThis work was undertaken to investígate the effect of variations of the feed rate on a fed-batch set-up used to degrade xenobiotics. The mixture of substrates was composed of PCP, 2,4,6 TCP and 2,3,5,6 TeCP (pentachlorophenol, 2,4,6 trichlo- rophenol and 2,3,5,6 tetrachlorophenol respectively). Two acclimated bacteria isolated from soil were used: Pseudomonas aeruginosa and Achromobacter sp. nov. The different flow rates tested were: I: 0.5 ml*min-1, II: 1.67 ml*min$latex ^{} $-1 and III: 2.00 mf-min'1. Our results show that during fed-batch operation the 2,4,6 TCP exhibits an earlier degradation than the other com- pounds, for all of the flow rates tested. This indicates that in this case the degradation of the most recalcitrant compounds (PCP and 2,3,5,6 TeCP) is benefited by the increase in biomass ofbacteria, due to the metabolisation of a less recalcitrant compound (2,4,6 TCP). The defined parameter, specific degradation rate (SDR), was demonstrated to be very useful for comparing the degradation abilities at different flow rates of a fed-batch system. The degradation efficiencies were shown to be higher than 90% for all of the cases and to decrease as the feed rate increases. However, the SDR, a parameter that involves the rate of degradation and the biomass, increases as the flow rate increases. At a feed flow rate of 2 mf-min'1 SDR reaches a maximum of 12.476 x 10'10 mgCP-M-CFU'1. Finally, among the feed flows tested, taking into account both the degradation efficiency and the SDR, 2 ml-min-1 is the most convenient flow rate for chlorophenol degradation in fed-batch Systems. An even higher degradation efficiency (97%) can be achieved by using the feed rate of 2 mf-min'1 followed by an additional batch post-treatment of2 h, with a SDR of 13.136 x 10-10 mg CP-h1-CFU1.Water Research Commission2011-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/4078enginfo:eu-repo/semantics/altIdentifier/doi/10.4314/wsa.v37i4.13info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:40:02Zoai:digital.cic.gba.gob.ar:11746/4078Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:40:03.181CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Degradation of chlorophenol mixtures in a fed-­batch system by two soil bacteria
title Degradation of chlorophenol mixtures in a fed-­batch system by two soil bacteria
spellingShingle Degradation of chlorophenol mixtures in a fed-­batch system by two soil bacteria
Durruty, Ignacio
Bioquímica y Biología Molecular
Clorofenoles
Biodegradación Ambiental
Biocatálisis
title_short Degradation of chlorophenol mixtures in a fed-­batch system by two soil bacteria
title_full Degradation of chlorophenol mixtures in a fed-­batch system by two soil bacteria
title_fullStr Degradation of chlorophenol mixtures in a fed-­batch system by two soil bacteria
title_full_unstemmed Degradation of chlorophenol mixtures in a fed-­batch system by two soil bacteria
title_sort Degradation of chlorophenol mixtures in a fed-­batch system by two soil bacteria
dc.creator.none.fl_str_mv Durruty, Ignacio
Okada, Elena
González, Jorge Froilán
Murialdo, Silvia Elena
author Durruty, Ignacio
author_facet Durruty, Ignacio
Okada, Elena
González, Jorge Froilán
Murialdo, Silvia Elena
author_role author
author2 Okada, Elena
González, Jorge Froilán
Murialdo, Silvia Elena
author2_role author
author
author
dc.subject.none.fl_str_mv Bioquímica y Biología Molecular
Clorofenoles
Biodegradación Ambiental
Biocatálisis
topic Bioquímica y Biología Molecular
Clorofenoles
Biodegradación Ambiental
Biocatálisis
dc.description.none.fl_txt_mv This work was undertaken to investígate the effect of variations of the feed rate on a fed-batch set-up used to degrade xenobiotics. The mixture of substrates was composed of PCP, 2,4,6 TCP and 2,3,5,6 TeCP (pentachlorophenol, 2,4,6 trichlo- rophenol and 2,3,5,6 tetrachlorophenol respectively). Two acclimated bacteria isolated from soil were used: Pseudomonas aeruginosa and Achromobacter sp. nov. The different flow rates tested were: I: 0.5 ml*min-1, II: 1.67 ml*min$latex ^{} $-1 and III: 2.00 mf-min'1. Our results show that during fed-batch operation the 2,4,6 TCP exhibits an earlier degradation than the other com- pounds, for all of the flow rates tested. This indicates that in this case the degradation of the most recalcitrant compounds (PCP and 2,3,5,6 TeCP) is benefited by the increase in biomass ofbacteria, due to the metabolisation of a less recalcitrant compound (2,4,6 TCP). The defined parameter, specific degradation rate (SDR), was demonstrated to be very useful for comparing the degradation abilities at different flow rates of a fed-batch system. The degradation efficiencies were shown to be higher than 90% for all of the cases and to decrease as the feed rate increases. However, the SDR, a parameter that involves the rate of degradation and the biomass, increases as the flow rate increases. At a feed flow rate of 2 mf-min'1 SDR reaches a maximum of 12.476 x 10'10 mgCP-M-CFU'1. Finally, among the feed flows tested, taking into account both the degradation efficiency and the SDR, 2 ml-min-1 is the most convenient flow rate for chlorophenol degradation in fed-batch Systems. An even higher degradation efficiency (97%) can be achieved by using the feed rate of 2 mf-min'1 followed by an additional batch post-treatment of2 h, with a SDR of 13.136 x 10-10 mg CP-h1-CFU1.
description This work was undertaken to investígate the effect of variations of the feed rate on a fed-batch set-up used to degrade xenobiotics. The mixture of substrates was composed of PCP, 2,4,6 TCP and 2,3,5,6 TeCP (pentachlorophenol, 2,4,6 trichlo- rophenol and 2,3,5,6 tetrachlorophenol respectively). Two acclimated bacteria isolated from soil were used: Pseudomonas aeruginosa and Achromobacter sp. nov. The different flow rates tested were: I: 0.5 ml*min-1, II: 1.67 ml*min$latex ^{} $-1 and III: 2.00 mf-min'1. Our results show that during fed-batch operation the 2,4,6 TCP exhibits an earlier degradation than the other com- pounds, for all of the flow rates tested. This indicates that in this case the degradation of the most recalcitrant compounds (PCP and 2,3,5,6 TeCP) is benefited by the increase in biomass ofbacteria, due to the metabolisation of a less recalcitrant compound (2,4,6 TCP). The defined parameter, specific degradation rate (SDR), was demonstrated to be very useful for comparing the degradation abilities at different flow rates of a fed-batch system. The degradation efficiencies were shown to be higher than 90% for all of the cases and to decrease as the feed rate increases. However, the SDR, a parameter that involves the rate of degradation and the biomass, increases as the flow rate increases. At a feed flow rate of 2 mf-min'1 SDR reaches a maximum of 12.476 x 10'10 mgCP-M-CFU'1. Finally, among the feed flows tested, taking into account both the degradation efficiency and the SDR, 2 ml-min-1 is the most convenient flow rate for chlorophenol degradation in fed-batch Systems. An even higher degradation efficiency (97%) can be achieved by using the feed rate of 2 mf-min'1 followed by an additional batch post-treatment of2 h, with a SDR of 13.136 x 10-10 mg CP-h1-CFU1.
publishDate 2011
dc.date.none.fl_str_mv 2011-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://digital.cic.gba.gob.ar/handle/11746/4078
url https://digital.cic.gba.gob.ar/handle/11746/4078
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4314/wsa.v37i4.13
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Water Research Commission
publisher.none.fl_str_mv Water Research Commission
dc.source.none.fl_str_mv reponame:CIC Digital (CICBA)
instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron:CICBA
reponame_str CIC Digital (CICBA)
collection CIC Digital (CICBA)
instname_str Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron_str CICBA
institution CICBA
repository.name.fl_str_mv CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
repository.mail.fl_str_mv marisa.degiusti@sedici.unlp.edu.ar
_version_ 1844618596088545280
score 13.070432