Partial molecular characterization of different proviral strains of bovine leukemia virus

Autores
Juliarena, Marcela A.; Lendez, Pamela A.; Gutierrez, Silvina E.; Forletti, Agustina; Rensetti, Daniel Ernesto; Ceriani, Maria Carolina
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión enviada
Descripción
Bovine leukemia virus (BLV)-infected cattle were classified by their proviral load into low and high proviral load profiles (LPL and HPL, respectively). Blood from these animals was used to infect sheep to obtain multiple identical copies of integrated provirus. An env fragment of BLV was amplified from all infected sheep and sequenced. The sequences that were obtained were compared to already published BLV genome sequence, resulting in three clusters. Mutations could not be attributed to the passage of provirus from cattle to sheep and subsequent amplification and sequencing. The description of two different proviral load profiles, the association of the BoLA-DRB3.2 0902 allele with the LPL profile, the availability of complete BLV sequences, and the comparison of a variable region of the env gene from carefully characterized cattle are still not enough to explain the presence of animals in every herd that are resistant to BLV dissemination.
Materia
Ciencias Veterinarias
Bovine leukemia virus (BLV)
BoLA-DRB3.2*0902 allele
env gene
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/5331

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oai_identifier_str oai:digital.cic.gba.gob.ar:11746/5331
network_acronym_str CICBA
repository_id_str 9441
network_name_str CIC Digital (CICBA)
spelling Partial molecular characterization of different proviral strains of bovine leukemia virusJuliarena, Marcela A.Lendez, Pamela A.Gutierrez, Silvina E.Forletti, AgustinaRensetti, Daniel ErnestoCeriani, Maria CarolinaCiencias VeterinariasBovine leukemia virus (BLV)BoLA-DRB3.2*0902 alleleenv geneBovine leukemia virus (BLV)-infected cattle were classified by their proviral load into low and high proviral load profiles (LPL and HPL, respectively). Blood from these animals was used to infect sheep to obtain multiple identical copies of integrated provirus. An env fragment of BLV was amplified from all infected sheep and sequenced. The sequences that were obtained were compared to already published BLV genome sequence, resulting in three clusters. Mutations could not be attributed to the passage of provirus from cattle to sheep and subsequent amplification and sequencing. The description of two different proviral load profiles, the association of the BoLA-DRB3.2 0902 allele with the LPL profile, the availability of complete BLV sequences, and the comparison of a variable region of the env gene from carefully characterized cattle are still not enough to explain the presence of animals in every herd that are resistant to BLV dissemination.2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/5331enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:40:18Zoai:digital.cic.gba.gob.ar:11746/5331Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:40:18.98CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Partial molecular characterization of different proviral strains of bovine leukemia virus
title Partial molecular characterization of different proviral strains of bovine leukemia virus
spellingShingle Partial molecular characterization of different proviral strains of bovine leukemia virus
Juliarena, Marcela A.
Ciencias Veterinarias
Bovine leukemia virus (BLV)
BoLA-DRB3.2*0902 allele
env gene
title_short Partial molecular characterization of different proviral strains of bovine leukemia virus
title_full Partial molecular characterization of different proviral strains of bovine leukemia virus
title_fullStr Partial molecular characterization of different proviral strains of bovine leukemia virus
title_full_unstemmed Partial molecular characterization of different proviral strains of bovine leukemia virus
title_sort Partial molecular characterization of different proviral strains of bovine leukemia virus
dc.creator.none.fl_str_mv Juliarena, Marcela A.
Lendez, Pamela A.
Gutierrez, Silvina E.
Forletti, Agustina
Rensetti, Daniel Ernesto
Ceriani, Maria Carolina
author Juliarena, Marcela A.
author_facet Juliarena, Marcela A.
Lendez, Pamela A.
Gutierrez, Silvina E.
Forletti, Agustina
Rensetti, Daniel Ernesto
Ceriani, Maria Carolina
author_role author
author2 Lendez, Pamela A.
Gutierrez, Silvina E.
Forletti, Agustina
Rensetti, Daniel Ernesto
Ceriani, Maria Carolina
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Bovine leukemia virus (BLV)
BoLA-DRB3.2*0902 allele
env gene
topic Ciencias Veterinarias
Bovine leukemia virus (BLV)
BoLA-DRB3.2*0902 allele
env gene
dc.description.none.fl_txt_mv Bovine leukemia virus (BLV)-infected cattle were classified by their proviral load into low and high proviral load profiles (LPL and HPL, respectively). Blood from these animals was used to infect sheep to obtain multiple identical copies of integrated provirus. An env fragment of BLV was amplified from all infected sheep and sequenced. The sequences that were obtained were compared to already published BLV genome sequence, resulting in three clusters. Mutations could not be attributed to the passage of provirus from cattle to sheep and subsequent amplification and sequencing. The description of two different proviral load profiles, the association of the BoLA-DRB3.2 0902 allele with the LPL profile, the availability of complete BLV sequences, and the comparison of a variable region of the env gene from carefully characterized cattle are still not enough to explain the presence of animals in every herd that are resistant to BLV dissemination.
description Bovine leukemia virus (BLV)-infected cattle were classified by their proviral load into low and high proviral load profiles (LPL and HPL, respectively). Blood from these animals was used to infect sheep to obtain multiple identical copies of integrated provirus. An env fragment of BLV was amplified from all infected sheep and sequenced. The sequences that were obtained were compared to already published BLV genome sequence, resulting in three clusters. Mutations could not be attributed to the passage of provirus from cattle to sheep and subsequent amplification and sequencing. The description of two different proviral load profiles, the association of the BoLA-DRB3.2 0902 allele with the LPL profile, the availability of complete BLV sequences, and the comparison of a variable region of the env gene from carefully characterized cattle are still not enough to explain the presence of animals in every herd that are resistant to BLV dissemination.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv https://digital.cic.gba.gob.ar/handle/11746/5331
url https://digital.cic.gba.gob.ar/handle/11746/5331
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:CIC Digital (CICBA)
instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron:CICBA
reponame_str CIC Digital (CICBA)
collection CIC Digital (CICBA)
instname_str Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron_str CICBA
institution CICBA
repository.name.fl_str_mv CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
repository.mail.fl_str_mv marisa.degiusti@sedici.unlp.edu.ar
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score 13.070432