Video processing analysis for the determination and evaluation of the chemotactic response in bacterial populations
- Autores
- Nisenbaum, Melina; Maldonado, Emilio; Martínez Arca, Jorge; González, Jorge F.; Passoni, Lucía I.; Murialdo, Silvia Elena
- Año de publicación
- 2016
- Idioma
- español castellano
- Tipo de recurso
- artículo
- Estado
- versión enviada
- Descripción
- The aim of the present work was to design a methodology based on video processing to obtain indicators of bacterial population motility that allow the quantitative and qualitative analysis and comparison of the chemotactic phenomenon with different attractants in the agarosein plug bridge method. Video image sequences were processed applying Shannon's entropy to the intensity time series of each pixel, which conducted to a final pseudo colored image resembling a map of the dynamic bacterial clusters. Processed images could discriminate perfectly between positive and negative attractant responses at different periods of time from the beginning of the assay. An index of spatial and temporal motility was proposed to quantify the bacterial response. With this index, this video processing method allowed obtaining quantitative information of the dynamic changes in space and time from a traditional qualitative assay. We conclude that this computational technique, applied to the traditional agarosein plug assay, has demonstrated good sensitivity for identifying chemotactic regions with a broad range of motility.
- Materia
-
Ciencias Químicas
bacteria
motility
video
chemotaxis - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-nd/4.0/
- Repositorio
- Institución
- Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
- OAI Identificador
- oai:digital.cic.gba.gob.ar:11746/4322
Ver los metadatos del registro completo
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CIC Digital (CICBA) |
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Video processing analysis for the determination and evaluation of the chemotactic response in bacterial populationsNisenbaum, MelinaMaldonado, EmilioMartínez Arca, JorgeGonzález, Jorge F.Passoni, Lucía I.Murialdo, Silvia ElenaCiencias QuímicasbacteriamotilityvideochemotaxisThe aim of the present work was to design a methodology based on video processing to obtain indicators of bacterial population motility that allow the quantitative and qualitative analysis and comparison of the chemotactic phenomenon with different attractants in the agarosein plug bridge method. Video image sequences were processed applying Shannon's entropy to the intensity time series of each pixel, which conducted to a final pseudo colored image resembling a map of the dynamic bacterial clusters. Processed images could discriminate perfectly between positive and negative attractant responses at different periods of time from the beginning of the assay. An index of spatial and temporal motility was proposed to quantify the bacterial response. With this index, this video processing method allowed obtaining quantitative information of the dynamic changes in space and time from a traditional qualitative assay. We conclude that this computational technique, applied to the traditional agarosein plug assay, has demonstrated good sensitivity for identifying chemotactic regions with a broad range of motility.2016-07-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/4322spainfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:40:24Zoai:digital.cic.gba.gob.ar:11746/4322Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:40:24.702CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse |
dc.title.none.fl_str_mv |
Video processing analysis for the determination and evaluation of the chemotactic response in bacterial populations |
title |
Video processing analysis for the determination and evaluation of the chemotactic response in bacterial populations |
spellingShingle |
Video processing analysis for the determination and evaluation of the chemotactic response in bacterial populations Nisenbaum, Melina Ciencias Químicas bacteria motility video chemotaxis |
title_short |
Video processing analysis for the determination and evaluation of the chemotactic response in bacterial populations |
title_full |
Video processing analysis for the determination and evaluation of the chemotactic response in bacterial populations |
title_fullStr |
Video processing analysis for the determination and evaluation of the chemotactic response in bacterial populations |
title_full_unstemmed |
Video processing analysis for the determination and evaluation of the chemotactic response in bacterial populations |
title_sort |
Video processing analysis for the determination and evaluation of the chemotactic response in bacterial populations |
dc.creator.none.fl_str_mv |
Nisenbaum, Melina Maldonado, Emilio Martínez Arca, Jorge González, Jorge F. Passoni, Lucía I. Murialdo, Silvia Elena |
author |
Nisenbaum, Melina |
author_facet |
Nisenbaum, Melina Maldonado, Emilio Martínez Arca, Jorge González, Jorge F. Passoni, Lucía I. Murialdo, Silvia Elena |
author_role |
author |
author2 |
Maldonado, Emilio Martínez Arca, Jorge González, Jorge F. Passoni, Lucía I. Murialdo, Silvia Elena |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Ciencias Químicas bacteria motility video chemotaxis |
topic |
Ciencias Químicas bacteria motility video chemotaxis |
dc.description.none.fl_txt_mv |
The aim of the present work was to design a methodology based on video processing to obtain indicators of bacterial population motility that allow the quantitative and qualitative analysis and comparison of the chemotactic phenomenon with different attractants in the agarosein plug bridge method. Video image sequences were processed applying Shannon's entropy to the intensity time series of each pixel, which conducted to a final pseudo colored image resembling a map of the dynamic bacterial clusters. Processed images could discriminate perfectly between positive and negative attractant responses at different periods of time from the beginning of the assay. An index of spatial and temporal motility was proposed to quantify the bacterial response. With this index, this video processing method allowed obtaining quantitative information of the dynamic changes in space and time from a traditional qualitative assay. We conclude that this computational technique, applied to the traditional agarosein plug assay, has demonstrated good sensitivity for identifying chemotactic regions with a broad range of motility. |
description |
The aim of the present work was to design a methodology based on video processing to obtain indicators of bacterial population motility that allow the quantitative and qualitative analysis and comparison of the chemotactic phenomenon with different attractants in the agarosein plug bridge method. Video image sequences were processed applying Shannon's entropy to the intensity time series of each pixel, which conducted to a final pseudo colored image resembling a map of the dynamic bacterial clusters. Processed images could discriminate perfectly between positive and negative attractant responses at different periods of time from the beginning of the assay. An index of spatial and temporal motility was proposed to quantify the bacterial response. With this index, this video processing method allowed obtaining quantitative information of the dynamic changes in space and time from a traditional qualitative assay. We conclude that this computational technique, applied to the traditional agarosein plug assay, has demonstrated good sensitivity for identifying chemotactic regions with a broad range of motility. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-07-20 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/submittedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
submittedVersion |
dc.identifier.none.fl_str_mv |
https://digital.cic.gba.gob.ar/handle/11746/4322 |
url |
https://digital.cic.gba.gob.ar/handle/11746/4322 |
dc.language.none.fl_str_mv |
spa |
language |
spa |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:CIC Digital (CICBA) instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires instacron:CICBA |
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CIC Digital (CICBA) |
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CIC Digital (CICBA) |
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Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
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CICBA |
institution |
CICBA |
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CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
repository.mail.fl_str_mv |
marisa.degiusti@sedici.unlp.edu.ar |
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13.070432 |