Hydrocarbon biodegradation and dynamic laser speckle for detecting chemotactic responses at low bacterial concentration

Autores
Nisenbaum, Melina; Sendra, Gonzalo Hernán; Gilbert, Gastón Alfredo Cerdá; Scagliola, Marcelo; González, Jorge Froilán; Murialdo, Silvia Elena
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We report on the biodegradation of pure hydrocarbons and chemotaxis towards these compounds by an isolated chlorophenol degrader, Pseudomonas strain H. The biochemical and phylogenetic analysis of the 16S rDNA sequence identified Pseudomonas strain H as having 99.56% similarity with P. aeruginosa PA01. This strain was able to degrade n-hexadecane, 1-undecene, 1-nonene, 1-decene, 1-dodecene and kerosene. It grew in the presence of 1-octene, while this hydrocarbons is toxic to other hydrocarbons degraders. Pseudomonas strain H was also chemotactic towards n-hexadecane, kerosene, 1-undecene and 1-dodecene. These results show that this Pseudomonas strain H is an attractive candidate for hydrocarbon-containing wastewater bioremediation in controlled environments. Since the classical standard techniques for detecting chemotaxis are not efficient at low bacterial concentrations, we demonstrate the use of the dynamic speckle laser method, which is simple and inexpensive, to confirm bacterial chemotaxis at low cell concentrations (less than 105 colony-forming unit per millilitre (CFU/mL)) when hydrocarbons are the attractants.
Fil: Nisenbaum, Melina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Sendra, Gonzalo Hernán. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Gilbert, Gastón Alfredo Cerdá. Obras Sanitarias Mar del Plata; Argentina
Fil: Scagliola, Marcelo. Obras Sanitarias Mar del Plata; Argentina
Fil: González, Jorge Froilán. Universidad Nacional de Mar del Plata; Argentina
Fil: Murialdo, Silvia Elena. Universidad Nacional de Mar del Plata; Argentina
Materia
BIODEGRADATION
CHEMOTAXIS
DYNAMIC LASER SPECKLE
HYDROCARBONS
MICROORGANISMS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195919
Acceder
id CONICETDig_3c35b543f5089039d5a3710c02eac35c
oai_identifier_str oai:ri.conicet.gov.ar:11336/195919
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Hydrocarbon biodegradation and dynamic laser speckle for detecting chemotactic responses at low bacterial concentrationNisenbaum, MelinaSendra, Gonzalo HernánGilbert, Gastón Alfredo CerdáScagliola, MarceloGonzález, Jorge FroilánMurialdo, Silvia ElenaBIODEGRADATIONCHEMOTAXISDYNAMIC LASER SPECKLEHYDROCARBONSMICROORGANISMShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1https://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2We report on the biodegradation of pure hydrocarbons and chemotaxis towards these compounds by an isolated chlorophenol degrader, Pseudomonas strain H. The biochemical and phylogenetic analysis of the 16S rDNA sequence identified Pseudomonas strain H as having 99.56% similarity with P. aeruginosa PA01. This strain was able to degrade n-hexadecane, 1-undecene, 1-nonene, 1-decene, 1-dodecene and kerosene. It grew in the presence of 1-octene, while this hydrocarbons is toxic to other hydrocarbons degraders. Pseudomonas strain H was also chemotactic towards n-hexadecane, kerosene, 1-undecene and 1-dodecene. These results show that this Pseudomonas strain H is an attractive candidate for hydrocarbon-containing wastewater bioremediation in controlled environments. Since the classical standard techniques for detecting chemotaxis are not efficient at low bacterial concentrations, we demonstrate the use of the dynamic speckle laser method, which is simple and inexpensive, to confirm bacterial chemotaxis at low cell concentrations (less than 105 colony-forming unit per millilitre (CFU/mL)) when hydrocarbons are the attractants.Fil: Nisenbaum, Melina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Sendra, Gonzalo Hernán. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Gilbert, Gastón Alfredo Cerdá. Obras Sanitarias Mar del Plata; ArgentinaFil: Scagliola, Marcelo. Obras Sanitarias Mar del Plata; ArgentinaFil: González, Jorge Froilán. Universidad Nacional de Mar del Plata; ArgentinaFil: Murialdo, Silvia Elena. Universidad Nacional de Mar del Plata; ArgentinaScience Press2012-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195919Nisenbaum, Melina; Sendra, Gonzalo Hernán; Gilbert, Gastón Alfredo Cerdá; Scagliola, Marcelo; González, Jorge Froilán; et al.; Hydrocarbon biodegradation and dynamic laser speckle for detecting chemotactic responses at low bacterial concentration; Science Press; Journal of Environmental Sciences; 25; 3; 3-2012; 613-6250022-0906CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/S1001-0742(12)60020-5info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1001074212600205info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:41:43Zoai:ri.conicet.gov.ar:11336/195919instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:41:43.893CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Hydrocarbon biodegradation and dynamic laser speckle for detecting chemotactic responses at low bacterial concentration
title Hydrocarbon biodegradation and dynamic laser speckle for detecting chemotactic responses at low bacterial concentration
spellingShingle Hydrocarbon biodegradation and dynamic laser speckle for detecting chemotactic responses at low bacterial concentration
Nisenbaum, Melina
BIODEGRADATION
CHEMOTAXIS
DYNAMIC LASER SPECKLE
HYDROCARBONS
MICROORGANISMS
title_short Hydrocarbon biodegradation and dynamic laser speckle for detecting chemotactic responses at low bacterial concentration
title_full Hydrocarbon biodegradation and dynamic laser speckle for detecting chemotactic responses at low bacterial concentration
title_fullStr Hydrocarbon biodegradation and dynamic laser speckle for detecting chemotactic responses at low bacterial concentration
title_full_unstemmed Hydrocarbon biodegradation and dynamic laser speckle for detecting chemotactic responses at low bacterial concentration
title_sort Hydrocarbon biodegradation and dynamic laser speckle for detecting chemotactic responses at low bacterial concentration
dc.creator.none.fl_str_mv Nisenbaum, Melina
Sendra, Gonzalo Hernán
Gilbert, Gastón Alfredo Cerdá
Scagliola, Marcelo
González, Jorge Froilán
Murialdo, Silvia Elena
author Nisenbaum, Melina
author_facet Nisenbaum, Melina
Sendra, Gonzalo Hernán
Gilbert, Gastón Alfredo Cerdá
Scagliola, Marcelo
González, Jorge Froilán
Murialdo, Silvia Elena
author_role author
author2 Sendra, Gonzalo Hernán
Gilbert, Gastón Alfredo Cerdá
Scagliola, Marcelo
González, Jorge Froilán
Murialdo, Silvia Elena
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BIODEGRADATION
CHEMOTAXIS
DYNAMIC LASER SPECKLE
HYDROCARBONS
MICROORGANISMS
topic BIODEGRADATION
CHEMOTAXIS
DYNAMIC LASER SPECKLE
HYDROCARBONS
MICROORGANISMS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv We report on the biodegradation of pure hydrocarbons and chemotaxis towards these compounds by an isolated chlorophenol degrader, Pseudomonas strain H. The biochemical and phylogenetic analysis of the 16S rDNA sequence identified Pseudomonas strain H as having 99.56% similarity with P. aeruginosa PA01. This strain was able to degrade n-hexadecane, 1-undecene, 1-nonene, 1-decene, 1-dodecene and kerosene. It grew in the presence of 1-octene, while this hydrocarbons is toxic to other hydrocarbons degraders. Pseudomonas strain H was also chemotactic towards n-hexadecane, kerosene, 1-undecene and 1-dodecene. These results show that this Pseudomonas strain H is an attractive candidate for hydrocarbon-containing wastewater bioremediation in controlled environments. Since the classical standard techniques for detecting chemotaxis are not efficient at low bacterial concentrations, we demonstrate the use of the dynamic speckle laser method, which is simple and inexpensive, to confirm bacterial chemotaxis at low cell concentrations (less than 105 colony-forming unit per millilitre (CFU/mL)) when hydrocarbons are the attractants.
Fil: Nisenbaum, Melina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Sendra, Gonzalo Hernán. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Gilbert, Gastón Alfredo Cerdá. Obras Sanitarias Mar del Plata; Argentina
Fil: Scagliola, Marcelo. Obras Sanitarias Mar del Plata; Argentina
Fil: González, Jorge Froilán. Universidad Nacional de Mar del Plata; Argentina
Fil: Murialdo, Silvia Elena. Universidad Nacional de Mar del Plata; Argentina
description We report on the biodegradation of pure hydrocarbons and chemotaxis towards these compounds by an isolated chlorophenol degrader, Pseudomonas strain H. The biochemical and phylogenetic analysis of the 16S rDNA sequence identified Pseudomonas strain H as having 99.56% similarity with P. aeruginosa PA01. This strain was able to degrade n-hexadecane, 1-undecene, 1-nonene, 1-decene, 1-dodecene and kerosene. It grew in the presence of 1-octene, while this hydrocarbons is toxic to other hydrocarbons degraders. Pseudomonas strain H was also chemotactic towards n-hexadecane, kerosene, 1-undecene and 1-dodecene. These results show that this Pseudomonas strain H is an attractive candidate for hydrocarbon-containing wastewater bioremediation in controlled environments. Since the classical standard techniques for detecting chemotaxis are not efficient at low bacterial concentrations, we demonstrate the use of the dynamic speckle laser method, which is simple and inexpensive, to confirm bacterial chemotaxis at low cell concentrations (less than 105 colony-forming unit per millilitre (CFU/mL)) when hydrocarbons are the attractants.
publishDate 2012
dc.date.none.fl_str_mv 2012-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195919
Nisenbaum, Melina; Sendra, Gonzalo Hernán; Gilbert, Gastón Alfredo Cerdá; Scagliola, Marcelo; González, Jorge Froilán; et al.; Hydrocarbon biodegradation and dynamic laser speckle for detecting chemotactic responses at low bacterial concentration; Science Press; Journal of Environmental Sciences; 25; 3; 3-2012; 613-625
0022-0906
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195919
identifier_str_mv Nisenbaum, Melina; Sendra, Gonzalo Hernán; Gilbert, Gastón Alfredo Cerdá; Scagliola, Marcelo; González, Jorge Froilán; et al.; Hydrocarbon biodegradation and dynamic laser speckle for detecting chemotactic responses at low bacterial concentration; Science Press; Journal of Environmental Sciences; 25; 3; 3-2012; 613-625
0022-0906
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/S1001-0742(12)60020-5
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1001074212600205
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Science Press
publisher.none.fl_str_mv Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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