Detection of Integrase Gene in E. coli Isolated from Pigs at Different Stages of Production System

Autores
Torre, Eulalia de la; Colello, Rocío; Padola, Nora Lía; Etcheverría, Analía Inés; Rodríguez, Edgardo; Amanto, Fabián; Tapia, María Ofelia; Soraci, Alejandro Luis
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Integrons are one of the genetic elements involved in the acquisition of antibiotic resistance. The aim of the present research is to investigate the presence of integrons in commensal Escherichia coli (E. coli) strains, isolated from pigs at different stages of production system and fromthe environment in an Argentinian farm. Five sows postpartumand five randomly chosen piglets from each litter were sampled by rectal swabs.They were sampled again at day 21 and at day 70. Environmental samples from the farm were also obtained. E. coli containing any integron class or combination of both integronswas detected by polymerase chain reaction in 100% of sows and in piglets at different stages of production: farrowing pen stage 68.1%;, weaning 60%, and growing/finishing 85.8%, showing an increase along the production system. From environmental samples 78.4% of E. coli containing any integron class was detected.We conclude that animals and farm environment can act as reservoirs for potential spread of resistant bacteria by means ofmobile genetic elements as integrons, which has amajor impact on production of food animals and that can reach man through the food chain, constituting a problem for public health.
Materia
Ciencias Veterinarias
Escherichia coli
Integrones
cerdos
medio ambiente
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/3.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/5744

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network_acronym_str CICBA
repository_id_str 9441
network_name_str CIC Digital (CICBA)
spelling Detection of Integrase Gene in E. coli Isolated from Pigs at Different Stages of Production SystemTorre, Eulalia de laColello, RocíoPadola, Nora LíaEtcheverría, Analía InésRodríguez, EdgardoAmanto, FabiánTapia, María OfeliaSoraci, Alejandro LuisCiencias VeterinariasEscherichia coliIntegronescerdosmedio ambienteIntegrons are one of the genetic elements involved in the acquisition of antibiotic resistance. The aim of the present research is to investigate the presence of integrons in commensal Escherichia coli (E. coli) strains, isolated from pigs at different stages of production system and fromthe environment in an Argentinian farm. Five sows postpartumand five randomly chosen piglets from each litter were sampled by rectal swabs.They were sampled again at day 21 and at day 70. Environmental samples from the farm were also obtained. E. coli containing any integron class or combination of both integronswas detected by polymerase chain reaction in 100% of sows and in piglets at different stages of production: farrowing pen stage 68.1%;, weaning 60%, and growing/finishing 85.8%, showing an increase along the production system. From environmental samples 78.4% of E. coli containing any integron class was detected.We conclude that animals and farm environment can act as reservoirs for potential spread of resistant bacteria by means ofmobile genetic elements as integrons, which has amajor impact on production of food animals and that can reach man through the food chain, constituting a problem for public health.Hindawi2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/5744enginfo:eu-repo/semantics/altIdentifier/doi/10.1155/2014/489569info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:40:02Zoai:digital.cic.gba.gob.ar:11746/5744Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:40:03.101CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Detection of Integrase Gene in E. coli Isolated from Pigs at Different Stages of Production System
title Detection of Integrase Gene in E. coli Isolated from Pigs at Different Stages of Production System
spellingShingle Detection of Integrase Gene in E. coli Isolated from Pigs at Different Stages of Production System
Torre, Eulalia de la
Ciencias Veterinarias
Escherichia coli
Integrones
cerdos
medio ambiente
title_short Detection of Integrase Gene in E. coli Isolated from Pigs at Different Stages of Production System
title_full Detection of Integrase Gene in E. coli Isolated from Pigs at Different Stages of Production System
title_fullStr Detection of Integrase Gene in E. coli Isolated from Pigs at Different Stages of Production System
title_full_unstemmed Detection of Integrase Gene in E. coli Isolated from Pigs at Different Stages of Production System
title_sort Detection of Integrase Gene in E. coli Isolated from Pigs at Different Stages of Production System
dc.creator.none.fl_str_mv Torre, Eulalia de la
Colello, Rocío
Padola, Nora Lía
Etcheverría, Analía Inés
Rodríguez, Edgardo
Amanto, Fabián
Tapia, María Ofelia
Soraci, Alejandro Luis
author Torre, Eulalia de la
author_facet Torre, Eulalia de la
Colello, Rocío
Padola, Nora Lía
Etcheverría, Analía Inés
Rodríguez, Edgardo
Amanto, Fabián
Tapia, María Ofelia
Soraci, Alejandro Luis
author_role author
author2 Colello, Rocío
Padola, Nora Lía
Etcheverría, Analía Inés
Rodríguez, Edgardo
Amanto, Fabián
Tapia, María Ofelia
Soraci, Alejandro Luis
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Escherichia coli
Integrones
cerdos
medio ambiente
topic Ciencias Veterinarias
Escherichia coli
Integrones
cerdos
medio ambiente
dc.description.none.fl_txt_mv Integrons are one of the genetic elements involved in the acquisition of antibiotic resistance. The aim of the present research is to investigate the presence of integrons in commensal Escherichia coli (E. coli) strains, isolated from pigs at different stages of production system and fromthe environment in an Argentinian farm. Five sows postpartumand five randomly chosen piglets from each litter were sampled by rectal swabs.They were sampled again at day 21 and at day 70. Environmental samples from the farm were also obtained. E. coli containing any integron class or combination of both integronswas detected by polymerase chain reaction in 100% of sows and in piglets at different stages of production: farrowing pen stage 68.1%;, weaning 60%, and growing/finishing 85.8%, showing an increase along the production system. From environmental samples 78.4% of E. coli containing any integron class was detected.We conclude that animals and farm environment can act as reservoirs for potential spread of resistant bacteria by means ofmobile genetic elements as integrons, which has amajor impact on production of food animals and that can reach man through the food chain, constituting a problem for public health.
description Integrons are one of the genetic elements involved in the acquisition of antibiotic resistance. The aim of the present research is to investigate the presence of integrons in commensal Escherichia coli (E. coli) strains, isolated from pigs at different stages of production system and fromthe environment in an Argentinian farm. Five sows postpartumand five randomly chosen piglets from each litter were sampled by rectal swabs.They were sampled again at day 21 and at day 70. Environmental samples from the farm were also obtained. E. coli containing any integron class or combination of both integronswas detected by polymerase chain reaction in 100% of sows and in piglets at different stages of production: farrowing pen stage 68.1%;, weaning 60%, and growing/finishing 85.8%, showing an increase along the production system. From environmental samples 78.4% of E. coli containing any integron class was detected.We conclude that animals and farm environment can act as reservoirs for potential spread of resistant bacteria by means ofmobile genetic elements as integrons, which has amajor impact on production of food animals and that can reach man through the food chain, constituting a problem for public health.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://digital.cic.gba.gob.ar/handle/11746/5744
url https://digital.cic.gba.gob.ar/handle/11746/5744
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1155/2014/489569
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hindawi
publisher.none.fl_str_mv Hindawi
dc.source.none.fl_str_mv reponame:CIC Digital (CICBA)
instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron:CICBA
reponame_str CIC Digital (CICBA)
collection CIC Digital (CICBA)
instname_str Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron_str CICBA
institution CICBA
repository.name.fl_str_mv CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
repository.mail.fl_str_mv marisa.degiusti@sedici.unlp.edu.ar
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