Laccases production by A.blazei mushroom grown either in composted or non-composted substrates

Autores
Gonzalez Matute, Ramiro; Figlas, Norma Débora; Curvetto, Néstor
Año de publicación
2013
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Agaricus blazei is an edible and medicinal mushroom commonly cultivated on compost. However, non-composted substrates are being particularly studied for specialty mushrooms because their economic and labour advantages. Addition of salt minerals to the substrate or casing materials may stimulate both the synthesis and activity of enzymes involved in the mushroom substrate biotransformation and eventually lead to an increase not only in mushroom productivity but in the fruitbody mineral content too. Remaining substrates from mushroom cultivation are a potential source for the extraction of high valued ligninolytic enzymes like laccases. The main objective of this study was to determine the laccase activity level produced by A. blazei on different phases of its growth cycle, using composted and non-composted substrates. It was also studied the A. blazei laccase production in substrates, either in the presence or in the absence of Cu2+ or Zn2+ (100 or 200 ppm). Results showed that laccase activity depends on the substrate used and varies along the different phases of cultivation. It was also demonstrated, that laccase production and its subsequent accumulation in a substrate are not necessarily correlated with the mycelia growth rates and mushroom production yields. The incorporation of Cu2+ (100 and 200 ppm) solutions on top of the casing layer (composted substrate) or as part of the formula (noncomposted substrates), stimulated the laccase production in the studied substrates. In the case of Zn2+, only the addition of 100 ppm had a positive effect on laccases and mainly in composted substrates.
Materia
Bioquímica y Biología Molecular
Almond portobello
Oligoelements
Bioremediation
White rot fungi enzymes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/4008

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repository_id_str 9441
network_name_str CIC Digital (CICBA)
spelling Laccases production by A.blazei mushroom grown either in composted or non-composted substratesGonzalez Matute, RamiroFiglas, Norma DéboraCurvetto, NéstorBioquímica y Biología MolecularAlmond portobelloOligoelementsBioremediationWhite rot fungi enzymesAgaricus blazei is an edible and medicinal mushroom commonly cultivated on compost. However, non-composted substrates are being particularly studied for specialty mushrooms because their economic and labour advantages. Addition of salt minerals to the substrate or casing materials may stimulate both the synthesis and activity of enzymes involved in the mushroom substrate biotransformation and eventually lead to an increase not only in mushroom productivity but in the fruitbody mineral content too. Remaining substrates from mushroom cultivation are a potential source for the extraction of high valued ligninolytic enzymes like laccases. The main objective of this study was to determine the laccase activity level produced by A. blazei on different phases of its growth cycle, using composted and non-composted substrates. It was also studied the A. blazei laccase production in substrates, either in the presence or in the absence of Cu2+ or Zn2+ (100 or 200 ppm). Results showed that laccase activity depends on the substrate used and varies along the different phases of cultivation. It was also demonstrated, that laccase production and its subsequent accumulation in a substrate are not necessarily correlated with the mycelia growth rates and mushroom production yields. The incorporation of Cu2+ (100 and 200 ppm) solutions on top of the casing layer (composted substrate) or as part of the formula (noncomposted substrates), stimulated the laccase production in the studied substrates. In the case of Zn2+, only the addition of 100 ppm had a positive effect on laccases and mainly in composted substrates.Trade Science Inc2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/4008spainfo:eu-repo/semantics/altIdentifier/issn/0974-7435info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:40:24Zoai:digital.cic.gba.gob.ar:11746/4008Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:40:24.467CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Laccases production by A.blazei mushroom grown either in composted or non-composted substrates
title Laccases production by A.blazei mushroom grown either in composted or non-composted substrates
spellingShingle Laccases production by A.blazei mushroom grown either in composted or non-composted substrates
Gonzalez Matute, Ramiro
Bioquímica y Biología Molecular
Almond portobello
Oligoelements
Bioremediation
White rot fungi enzymes
title_short Laccases production by A.blazei mushroom grown either in composted or non-composted substrates
title_full Laccases production by A.blazei mushroom grown either in composted or non-composted substrates
title_fullStr Laccases production by A.blazei mushroom grown either in composted or non-composted substrates
title_full_unstemmed Laccases production by A.blazei mushroom grown either in composted or non-composted substrates
title_sort Laccases production by A.blazei mushroom grown either in composted or non-composted substrates
dc.creator.none.fl_str_mv Gonzalez Matute, Ramiro
Figlas, Norma Débora
Curvetto, Néstor
author Gonzalez Matute, Ramiro
author_facet Gonzalez Matute, Ramiro
Figlas, Norma Débora
Curvetto, Néstor
author_role author
author2 Figlas, Norma Débora
Curvetto, Néstor
author2_role author
author
dc.subject.none.fl_str_mv Bioquímica y Biología Molecular
Almond portobello
Oligoelements
Bioremediation
White rot fungi enzymes
topic Bioquímica y Biología Molecular
Almond portobello
Oligoelements
Bioremediation
White rot fungi enzymes
dc.description.none.fl_txt_mv Agaricus blazei is an edible and medicinal mushroom commonly cultivated on compost. However, non-composted substrates are being particularly studied for specialty mushrooms because their economic and labour advantages. Addition of salt minerals to the substrate or casing materials may stimulate both the synthesis and activity of enzymes involved in the mushroom substrate biotransformation and eventually lead to an increase not only in mushroom productivity but in the fruitbody mineral content too. Remaining substrates from mushroom cultivation are a potential source for the extraction of high valued ligninolytic enzymes like laccases. The main objective of this study was to determine the laccase activity level produced by A. blazei on different phases of its growth cycle, using composted and non-composted substrates. It was also studied the A. blazei laccase production in substrates, either in the presence or in the absence of Cu2+ or Zn2+ (100 or 200 ppm). Results showed that laccase activity depends on the substrate used and varies along the different phases of cultivation. It was also demonstrated, that laccase production and its subsequent accumulation in a substrate are not necessarily correlated with the mycelia growth rates and mushroom production yields. The incorporation of Cu2+ (100 and 200 ppm) solutions on top of the casing layer (composted substrate) or as part of the formula (noncomposted substrates), stimulated the laccase production in the studied substrates. In the case of Zn2+, only the addition of 100 ppm had a positive effect on laccases and mainly in composted substrates.
description Agaricus blazei is an edible and medicinal mushroom commonly cultivated on compost. However, non-composted substrates are being particularly studied for specialty mushrooms because their economic and labour advantages. Addition of salt minerals to the substrate or casing materials may stimulate both the synthesis and activity of enzymes involved in the mushroom substrate biotransformation and eventually lead to an increase not only in mushroom productivity but in the fruitbody mineral content too. Remaining substrates from mushroom cultivation are a potential source for the extraction of high valued ligninolytic enzymes like laccases. The main objective of this study was to determine the laccase activity level produced by A. blazei on different phases of its growth cycle, using composted and non-composted substrates. It was also studied the A. blazei laccase production in substrates, either in the presence or in the absence of Cu2+ or Zn2+ (100 or 200 ppm). Results showed that laccase activity depends on the substrate used and varies along the different phases of cultivation. It was also demonstrated, that laccase production and its subsequent accumulation in a substrate are not necessarily correlated with the mycelia growth rates and mushroom production yields. The incorporation of Cu2+ (100 and 200 ppm) solutions on top of the casing layer (composted substrate) or as part of the formula (noncomposted substrates), stimulated the laccase production in the studied substrates. In the case of Zn2+, only the addition of 100 ppm had a positive effect on laccases and mainly in composted substrates.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://digital.cic.gba.gob.ar/handle/11746/4008
url https://digital.cic.gba.gob.ar/handle/11746/4008
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0974-7435
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Trade Science Inc
publisher.none.fl_str_mv Trade Science Inc
dc.source.none.fl_str_mv reponame:CIC Digital (CICBA)
instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron:CICBA
reponame_str CIC Digital (CICBA)
collection CIC Digital (CICBA)
instname_str Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron_str CICBA
institution CICBA
repository.name.fl_str_mv CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
repository.mail.fl_str_mv marisa.degiusti@sedici.unlp.edu.ar
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