Effect of erythropoietin on staurosporine-induced apoptosis and differentiation of SH-SY5Y neuroblastoma cells

Autores
Pregi, N.; Vittori, D.; Pérez, G.; Leirós, C.P.; Nesse, A.
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Since apoptosis appeared to be related to neurodegenerative processes, neuroprotection has been involved in investigation of therapeutic approaches focused upon pharmacological agents to prevent neuronal programmed cell death. In this regard, erythropoietin (Epo) seems to play a critical role. The present work was focused on the study of the Epo protective effect upon human neuroblastoma SH-SY5Y cells subjected to differentiation by staurosporine. Under this condition, profuse neurite outgrowth was accompanied by programmed cell death (35% of apoptotic cells by Hoechst assay, showing characteristic DNA ladder pattern). A previous treatment with recombinant human Epo (rHuEpo) increased the expression of the specific receptor for Epo while prevented apoptosis. Simultaneously, morphological changes in neurite elongation and interconnection induced by staurosporine were blocked by Epo. These Epo effects proved to be associated to the induction of Bcl-xL at the mRNA and protein levels (RT-PCR and Western blot after immunoprecipitation) and were mediated by activation of pathways inhibited by wortmannin. In conclusion, the fact that both events induced by staurosporine, cell apoptosis and differentiation, were prevented in SH-SY5Y cells previously exposed to rHuEpo suggests interrelated signaling pathways triggered by the Epo/EpoR interaction. © 2005 Elsevier B.V. All rights reserved.
Fil:Pregi, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Vittori, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Pérez, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Leirós, C.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Nesse, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
Biochim. Biophys. Acta Mol. Cell Res. 2006;1763(2):238-246
Materia
Apoptosis
Bcl-X
Erythropoietin
Neuroprotection
SH-SY5Y cell
Staurosporine
erythropoietin
protein bcl xl
staurosporine
apoptosis
article
cell death
cell differentiation
cell growth
cell structure
controlled study
human
human cell
immunoprecipitation
neurite
neuroblastoma cell
priority journal
reverse transcription polymerase chain reaction
Western blotting
Apoptosis
bcl-X Protein
Blotting, Western
Cell Differentiation
Dose-Response Relationship, Drug
Electrophoresis
Enzyme Inhibitors
Erythropoietin
Erythropoietin, Recombinant
Humans
Kinetics
Microscopy, Fluorescence
Neuroblastoma
Precipitin Tests
Receptors, Erythropoietin
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger
Staurosporine
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_01674889_v1763_n2_p238_Pregi
Acceder
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oai_identifier_str paperaa:paper_01674889_v1763_n2_p238_Pregi
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Effect of erythropoietin on staurosporine-induced apoptosis and differentiation of SH-SY5Y neuroblastoma cellsPregi, N.Vittori, D.Pérez, G.Leirós, C.P.Nesse, A.ApoptosisBcl-XErythropoietinNeuroprotectionSH-SY5Y cellStaurosporineerythropoietinprotein bcl xlstaurosporineapoptosisarticlecell deathcell differentiationcell growthcell structurecontrolled studyhumanhuman cellimmunoprecipitationneuriteneuroblastoma cellpriority journalreverse transcription polymerase chain reactionWestern blottingApoptosisbcl-X ProteinBlotting, WesternCell DifferentiationDose-Response Relationship, DrugElectrophoresisEnzyme InhibitorsErythropoietinErythropoietin, RecombinantHumansKineticsMicroscopy, FluorescenceNeuroblastomaPrecipitin TestsReceptors, ErythropoietinReverse Transcriptase Polymerase Chain ReactionRNA, MessengerStaurosporineSince apoptosis appeared to be related to neurodegenerative processes, neuroprotection has been involved in investigation of therapeutic approaches focused upon pharmacological agents to prevent neuronal programmed cell death. In this regard, erythropoietin (Epo) seems to play a critical role. The present work was focused on the study of the Epo protective effect upon human neuroblastoma SH-SY5Y cells subjected to differentiation by staurosporine. Under this condition, profuse neurite outgrowth was accompanied by programmed cell death (35% of apoptotic cells by Hoechst assay, showing characteristic DNA ladder pattern). A previous treatment with recombinant human Epo (rHuEpo) increased the expression of the specific receptor for Epo while prevented apoptosis. Simultaneously, morphological changes in neurite elongation and interconnection induced by staurosporine were blocked by Epo. These Epo effects proved to be associated to the induction of Bcl-xL at the mRNA and protein levels (RT-PCR and Western blot after immunoprecipitation) and were mediated by activation of pathways inhibited by wortmannin. In conclusion, the fact that both events induced by staurosporine, cell apoptosis and differentiation, were prevented in SH-SY5Y cells previously exposed to rHuEpo suggests interrelated signaling pathways triggered by the Epo/EpoR interaction. © 2005 Elsevier B.V. All rights reserved.Fil:Pregi, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Vittori, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Pérez, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Leirós, C.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Nesse, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2006info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_01674889_v1763_n2_p238_PregiBiochim. Biophys. Acta Mol. Cell Res. 2006;1763(2):238-246reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-04T09:48:44Zpaperaa:paper_01674889_v1763_n2_p238_PregiInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-04 09:48:46.08Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Effect of erythropoietin on staurosporine-induced apoptosis and differentiation of SH-SY5Y neuroblastoma cells
title Effect of erythropoietin on staurosporine-induced apoptosis and differentiation of SH-SY5Y neuroblastoma cells
spellingShingle Effect of erythropoietin on staurosporine-induced apoptosis and differentiation of SH-SY5Y neuroblastoma cells
Pregi, N.
Apoptosis
Bcl-X
Erythropoietin
Neuroprotection
SH-SY5Y cell
Staurosporine
erythropoietin
protein bcl xl
staurosporine
apoptosis
article
cell death
cell differentiation
cell growth
cell structure
controlled study
human
human cell
immunoprecipitation
neurite
neuroblastoma cell
priority journal
reverse transcription polymerase chain reaction
Western blotting
Apoptosis
bcl-X Protein
Blotting, Western
Cell Differentiation
Dose-Response Relationship, Drug
Electrophoresis
Enzyme Inhibitors
Erythropoietin
Erythropoietin, Recombinant
Humans
Kinetics
Microscopy, Fluorescence
Neuroblastoma
Precipitin Tests
Receptors, Erythropoietin
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger
Staurosporine
title_short Effect of erythropoietin on staurosporine-induced apoptosis and differentiation of SH-SY5Y neuroblastoma cells
title_full Effect of erythropoietin on staurosporine-induced apoptosis and differentiation of SH-SY5Y neuroblastoma cells
title_fullStr Effect of erythropoietin on staurosporine-induced apoptosis and differentiation of SH-SY5Y neuroblastoma cells
title_full_unstemmed Effect of erythropoietin on staurosporine-induced apoptosis and differentiation of SH-SY5Y neuroblastoma cells
title_sort Effect of erythropoietin on staurosporine-induced apoptosis and differentiation of SH-SY5Y neuroblastoma cells
dc.creator.none.fl_str_mv Pregi, N.
Vittori, D.
Pérez, G.
Leirós, C.P.
Nesse, A.
author Pregi, N.
author_facet Pregi, N.
Vittori, D.
Pérez, G.
Leirós, C.P.
Nesse, A.
author_role author
author2 Vittori, D.
Pérez, G.
Leirós, C.P.
Nesse, A.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Apoptosis
Bcl-X
Erythropoietin
Neuroprotection
SH-SY5Y cell
Staurosporine
erythropoietin
protein bcl xl
staurosporine
apoptosis
article
cell death
cell differentiation
cell growth
cell structure
controlled study
human
human cell
immunoprecipitation
neurite
neuroblastoma cell
priority journal
reverse transcription polymerase chain reaction
Western blotting
Apoptosis
bcl-X Protein
Blotting, Western
Cell Differentiation
Dose-Response Relationship, Drug
Electrophoresis
Enzyme Inhibitors
Erythropoietin
Erythropoietin, Recombinant
Humans
Kinetics
Microscopy, Fluorescence
Neuroblastoma
Precipitin Tests
Receptors, Erythropoietin
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger
Staurosporine
topic Apoptosis
Bcl-X
Erythropoietin
Neuroprotection
SH-SY5Y cell
Staurosporine
erythropoietin
protein bcl xl
staurosporine
apoptosis
article
cell death
cell differentiation
cell growth
cell structure
controlled study
human
human cell
immunoprecipitation
neurite
neuroblastoma cell
priority journal
reverse transcription polymerase chain reaction
Western blotting
Apoptosis
bcl-X Protein
Blotting, Western
Cell Differentiation
Dose-Response Relationship, Drug
Electrophoresis
Enzyme Inhibitors
Erythropoietin
Erythropoietin, Recombinant
Humans
Kinetics
Microscopy, Fluorescence
Neuroblastoma
Precipitin Tests
Receptors, Erythropoietin
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger
Staurosporine
dc.description.none.fl_txt_mv Since apoptosis appeared to be related to neurodegenerative processes, neuroprotection has been involved in investigation of therapeutic approaches focused upon pharmacological agents to prevent neuronal programmed cell death. In this regard, erythropoietin (Epo) seems to play a critical role. The present work was focused on the study of the Epo protective effect upon human neuroblastoma SH-SY5Y cells subjected to differentiation by staurosporine. Under this condition, profuse neurite outgrowth was accompanied by programmed cell death (35% of apoptotic cells by Hoechst assay, showing characteristic DNA ladder pattern). A previous treatment with recombinant human Epo (rHuEpo) increased the expression of the specific receptor for Epo while prevented apoptosis. Simultaneously, morphological changes in neurite elongation and interconnection induced by staurosporine were blocked by Epo. These Epo effects proved to be associated to the induction of Bcl-xL at the mRNA and protein levels (RT-PCR and Western blot after immunoprecipitation) and were mediated by activation of pathways inhibited by wortmannin. In conclusion, the fact that both events induced by staurosporine, cell apoptosis and differentiation, were prevented in SH-SY5Y cells previously exposed to rHuEpo suggests interrelated signaling pathways triggered by the Epo/EpoR interaction. © 2005 Elsevier B.V. All rights reserved.
Fil:Pregi, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Vittori, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Pérez, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Leirós, C.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Nesse, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description Since apoptosis appeared to be related to neurodegenerative processes, neuroprotection has been involved in investigation of therapeutic approaches focused upon pharmacological agents to prevent neuronal programmed cell death. In this regard, erythropoietin (Epo) seems to play a critical role. The present work was focused on the study of the Epo protective effect upon human neuroblastoma SH-SY5Y cells subjected to differentiation by staurosporine. Under this condition, profuse neurite outgrowth was accompanied by programmed cell death (35% of apoptotic cells by Hoechst assay, showing characteristic DNA ladder pattern). A previous treatment with recombinant human Epo (rHuEpo) increased the expression of the specific receptor for Epo while prevented apoptosis. Simultaneously, morphological changes in neurite elongation and interconnection induced by staurosporine were blocked by Epo. These Epo effects proved to be associated to the induction of Bcl-xL at the mRNA and protein levels (RT-PCR and Western blot after immunoprecipitation) and were mediated by activation of pathways inhibited by wortmannin. In conclusion, the fact that both events induced by staurosporine, cell apoptosis and differentiation, were prevented in SH-SY5Y cells previously exposed to rHuEpo suggests interrelated signaling pathways triggered by the Epo/EpoR interaction. © 2005 Elsevier B.V. All rights reserved.
publishDate 2006
dc.date.none.fl_str_mv 2006
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_01674889_v1763_n2_p238_Pregi
url http://hdl.handle.net/20.500.12110/paper_01674889_v1763_n2_p238_Pregi
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Biochim. Biophys. Acta Mol. Cell Res. 2006;1763(2):238-246
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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score 12.623145

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