Effects of granule-associated protein PhaP on glycerol-dependent growth and polymer production in poly(3-hydroxybutyrate)-producing Escherichia coli

Autores
De Almeida, A.; Nikel, P.I.; Giordano, A.M.; Pettinari, M.J.
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Polyhydroxyalkanoates (PHAs) are accumulated as intracellular granules by many bacteria under unfavorable conditions, enhancing their fitness and stress resistance. Poly(3-hydroxybutyrate) (PHB) is the most widespread and best-known PHA. Apart from the genes that catalyze polymer biosynthesis, natural PHA producers have several genes for proteins involved in granule formation and/or with regulatory functions, such as phasins, that have been shown to affect polymer synthesis. This study evaluates the effect of PhaP, a phasin, on bacterial growth and PHB accumulation from glycerol in bioreactor cultures of recombinant Escherichia coli carrying phaBAC from Azotobacter sp. strain FA8. Cells expressing phaP grew more, and accumulated more PHB, both using glucose and using glycerol as carbon sources. When cultures were grown in a bioreactor using glycerol, PhaP-bearing cells produced more polymer (2.6 times) and more biomass (1.9 times) than did those without the phasin. The effect of this protein on growth promotion and polymer accumulation is expected to be even greater in high-density cultures, such as those used in the industrial production of the polymer. The recombinant strain presented in this work has been successfully used for the production of PHB from glycerol in bioreactor studies, allowing the production of 7.9 g/liter of the polymer in a semisynthetic medium in 48-h batch cultures. The development of bacterial strains that can efficiently use this substrate can help to make the industrial production of PHAs economically feasible. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Fil:De Almeida, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
Appl. Environ. Microbiol. 2007;73(24):7912-7916
Materia
Bacteria
Biomass
Bioreactors
Biosynthesis
Escherichia coli
Glycerol
Intracellular granules
Polyhydroxyalkanoates (PHAs)
Semisynthetic medium
Stress resistance
Proteins
bacterial protein
carbon
glucose
glycerol
poly(3 hydroxybutyric acid)
polyhydroxyalkanoic acid
polymer
regulator protein
bacterium
fitness
gene
growth
polymer
protein
stress resistance
article
Azotobacter
bacterial growth
bacterial strain
bacterium culture
bioreactor
biosynthesis
catalysis
cell granule
controlled study
Escherichia coli
nonhuman
protein expression
protein function
stress
Azotobacter
Bacterial Proteins
Biomass
Bioreactors
DNA-Binding Proteins
Escherichia coli
Fermentation
Glucose
Glycerol
Hydroxybutyrates
Polyesters
Azotobacter
Bacteria (microorganisms)
Escherichia coli
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_00992240_v73_n24_p7912_DeAlmeida

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oai_identifier_str paperaa:paper_00992240_v73_n24_p7912_DeAlmeida
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Effects of granule-associated protein PhaP on glycerol-dependent growth and polymer production in poly(3-hydroxybutyrate)-producing Escherichia coliDe Almeida, A.Nikel, P.I.Giordano, A.M.Pettinari, M.J.BacteriaBiomassBioreactorsBiosynthesisEscherichia coliGlycerolIntracellular granulesPolyhydroxyalkanoates (PHAs)Semisynthetic mediumStress resistanceProteinsbacterial proteincarbonglucoseglycerolpoly(3 hydroxybutyric acid)polyhydroxyalkanoic acidpolymerregulator proteinbacteriumfitnessgenegrowthpolymerproteinstress resistancearticleAzotobacterbacterial growthbacterial strainbacterium culturebioreactorbiosynthesiscatalysiscell granulecontrolled studyEscherichia colinonhumanprotein expressionprotein functionstressAzotobacterBacterial ProteinsBiomassBioreactorsDNA-Binding ProteinsEscherichia coliFermentationGlucoseGlycerolHydroxybutyratesPolyestersAzotobacterBacteria (microorganisms)Escherichia coliPolyhydroxyalkanoates (PHAs) are accumulated as intracellular granules by many bacteria under unfavorable conditions, enhancing their fitness and stress resistance. Poly(3-hydroxybutyrate) (PHB) is the most widespread and best-known PHA. Apart from the genes that catalyze polymer biosynthesis, natural PHA producers have several genes for proteins involved in granule formation and/or with regulatory functions, such as phasins, that have been shown to affect polymer synthesis. This study evaluates the effect of PhaP, a phasin, on bacterial growth and PHB accumulation from glycerol in bioreactor cultures of recombinant Escherichia coli carrying phaBAC from Azotobacter sp. strain FA8. Cells expressing phaP grew more, and accumulated more PHB, both using glucose and using glycerol as carbon sources. When cultures were grown in a bioreactor using glycerol, PhaP-bearing cells produced more polymer (2.6 times) and more biomass (1.9 times) than did those without the phasin. The effect of this protein on growth promotion and polymer accumulation is expected to be even greater in high-density cultures, such as those used in the industrial production of the polymer. The recombinant strain presented in this work has been successfully used for the production of PHB from glycerol in bioreactor studies, allowing the production of 7.9 g/liter of the polymer in a semisynthetic medium in 48-h batch cultures. The development of bacterial strains that can efficiently use this substrate can help to make the industrial production of PHAs economically feasible. Copyright © 2007, American Society for Microbiology. All Rights Reserved.Fil:De Almeida, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2007info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00992240_v73_n24_p7912_DeAlmeidaAppl. Environ. Microbiol. 2007;73(24):7912-7916reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-04T09:48:42Zpaperaa:paper_00992240_v73_n24_p7912_DeAlmeidaInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-04 09:48:43.327Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Effects of granule-associated protein PhaP on glycerol-dependent growth and polymer production in poly(3-hydroxybutyrate)-producing Escherichia coli
title Effects of granule-associated protein PhaP on glycerol-dependent growth and polymer production in poly(3-hydroxybutyrate)-producing Escherichia coli
spellingShingle Effects of granule-associated protein PhaP on glycerol-dependent growth and polymer production in poly(3-hydroxybutyrate)-producing Escherichia coli
De Almeida, A.
Bacteria
Biomass
Bioreactors
Biosynthesis
Escherichia coli
Glycerol
Intracellular granules
Polyhydroxyalkanoates (PHAs)
Semisynthetic medium
Stress resistance
Proteins
bacterial protein
carbon
glucose
glycerol
poly(3 hydroxybutyric acid)
polyhydroxyalkanoic acid
polymer
regulator protein
bacterium
fitness
gene
growth
polymer
protein
stress resistance
article
Azotobacter
bacterial growth
bacterial strain
bacterium culture
bioreactor
biosynthesis
catalysis
cell granule
controlled study
Escherichia coli
nonhuman
protein expression
protein function
stress
Azotobacter
Bacterial Proteins
Biomass
Bioreactors
DNA-Binding Proteins
Escherichia coli
Fermentation
Glucose
Glycerol
Hydroxybutyrates
Polyesters
Azotobacter
Bacteria (microorganisms)
Escherichia coli
title_short Effects of granule-associated protein PhaP on glycerol-dependent growth and polymer production in poly(3-hydroxybutyrate)-producing Escherichia coli
title_full Effects of granule-associated protein PhaP on glycerol-dependent growth and polymer production in poly(3-hydroxybutyrate)-producing Escherichia coli
title_fullStr Effects of granule-associated protein PhaP on glycerol-dependent growth and polymer production in poly(3-hydroxybutyrate)-producing Escherichia coli
title_full_unstemmed Effects of granule-associated protein PhaP on glycerol-dependent growth and polymer production in poly(3-hydroxybutyrate)-producing Escherichia coli
title_sort Effects of granule-associated protein PhaP on glycerol-dependent growth and polymer production in poly(3-hydroxybutyrate)-producing Escherichia coli
dc.creator.none.fl_str_mv De Almeida, A.
Nikel, P.I.
Giordano, A.M.
Pettinari, M.J.
author De Almeida, A.
author_facet De Almeida, A.
Nikel, P.I.
Giordano, A.M.
Pettinari, M.J.
author_role author
author2 Nikel, P.I.
Giordano, A.M.
Pettinari, M.J.
author2_role author
author
author
dc.subject.none.fl_str_mv Bacteria
Biomass
Bioreactors
Biosynthesis
Escherichia coli
Glycerol
Intracellular granules
Polyhydroxyalkanoates (PHAs)
Semisynthetic medium
Stress resistance
Proteins
bacterial protein
carbon
glucose
glycerol
poly(3 hydroxybutyric acid)
polyhydroxyalkanoic acid
polymer
regulator protein
bacterium
fitness
gene
growth
polymer
protein
stress resistance
article
Azotobacter
bacterial growth
bacterial strain
bacterium culture
bioreactor
biosynthesis
catalysis
cell granule
controlled study
Escherichia coli
nonhuman
protein expression
protein function
stress
Azotobacter
Bacterial Proteins
Biomass
Bioreactors
DNA-Binding Proteins
Escherichia coli
Fermentation
Glucose
Glycerol
Hydroxybutyrates
Polyesters
Azotobacter
Bacteria (microorganisms)
Escherichia coli
topic Bacteria
Biomass
Bioreactors
Biosynthesis
Escherichia coli
Glycerol
Intracellular granules
Polyhydroxyalkanoates (PHAs)
Semisynthetic medium
Stress resistance
Proteins
bacterial protein
carbon
glucose
glycerol
poly(3 hydroxybutyric acid)
polyhydroxyalkanoic acid
polymer
regulator protein
bacterium
fitness
gene
growth
polymer
protein
stress resistance
article
Azotobacter
bacterial growth
bacterial strain
bacterium culture
bioreactor
biosynthesis
catalysis
cell granule
controlled study
Escherichia coli
nonhuman
protein expression
protein function
stress
Azotobacter
Bacterial Proteins
Biomass
Bioreactors
DNA-Binding Proteins
Escherichia coli
Fermentation
Glucose
Glycerol
Hydroxybutyrates
Polyesters
Azotobacter
Bacteria (microorganisms)
Escherichia coli
dc.description.none.fl_txt_mv Polyhydroxyalkanoates (PHAs) are accumulated as intracellular granules by many bacteria under unfavorable conditions, enhancing their fitness and stress resistance. Poly(3-hydroxybutyrate) (PHB) is the most widespread and best-known PHA. Apart from the genes that catalyze polymer biosynthesis, natural PHA producers have several genes for proteins involved in granule formation and/or with regulatory functions, such as phasins, that have been shown to affect polymer synthesis. This study evaluates the effect of PhaP, a phasin, on bacterial growth and PHB accumulation from glycerol in bioreactor cultures of recombinant Escherichia coli carrying phaBAC from Azotobacter sp. strain FA8. Cells expressing phaP grew more, and accumulated more PHB, both using glucose and using glycerol as carbon sources. When cultures were grown in a bioreactor using glycerol, PhaP-bearing cells produced more polymer (2.6 times) and more biomass (1.9 times) than did those without the phasin. The effect of this protein on growth promotion and polymer accumulation is expected to be even greater in high-density cultures, such as those used in the industrial production of the polymer. The recombinant strain presented in this work has been successfully used for the production of PHB from glycerol in bioreactor studies, allowing the production of 7.9 g/liter of the polymer in a semisynthetic medium in 48-h batch cultures. The development of bacterial strains that can efficiently use this substrate can help to make the industrial production of PHAs economically feasible. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Fil:De Almeida, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description Polyhydroxyalkanoates (PHAs) are accumulated as intracellular granules by many bacteria under unfavorable conditions, enhancing their fitness and stress resistance. Poly(3-hydroxybutyrate) (PHB) is the most widespread and best-known PHA. Apart from the genes that catalyze polymer biosynthesis, natural PHA producers have several genes for proteins involved in granule formation and/or with regulatory functions, such as phasins, that have been shown to affect polymer synthesis. This study evaluates the effect of PhaP, a phasin, on bacterial growth and PHB accumulation from glycerol in bioreactor cultures of recombinant Escherichia coli carrying phaBAC from Azotobacter sp. strain FA8. Cells expressing phaP grew more, and accumulated more PHB, both using glucose and using glycerol as carbon sources. When cultures were grown in a bioreactor using glycerol, PhaP-bearing cells produced more polymer (2.6 times) and more biomass (1.9 times) than did those without the phasin. The effect of this protein on growth promotion and polymer accumulation is expected to be even greater in high-density cultures, such as those used in the industrial production of the polymer. The recombinant strain presented in this work has been successfully used for the production of PHB from glycerol in bioreactor studies, allowing the production of 7.9 g/liter of the polymer in a semisynthetic medium in 48-h batch cultures. The development of bacterial strains that can efficiently use this substrate can help to make the industrial production of PHAs economically feasible. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
publishDate 2007
dc.date.none.fl_str_mv 2007
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_00992240_v73_n24_p7912_DeAlmeida
url http://hdl.handle.net/20.500.12110/paper_00992240_v73_n24_p7912_DeAlmeida
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Appl. Environ. Microbiol. 2007;73(24):7912-7916
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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