Effects of aeration on the synthesis of poly(3-hydroxybutyrate) from glycerol and glucose in recombinant Escherichia coli

Autores
De Almeida, A.; Giordano, A.M.; Nikel, P.I.; Pettinari, M.J.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bioreactor cultures of Escherichia coli recombinants carrying phaBAC and phaP of Azotobacter sp. FA8 grown on glycerol under low-agitation conditions accumulated more poly(3-hydroxybutyrate) (PHB) and ethanol than at high agitation, while in glucose cultures, low agitation led to a decrease in PHB formation. Cells produced smaller amounts of acids from glycerol than from glucose. Glycerol batch cultures stirred at 125 rpm accumulated, in 24 h, 30.1% (wt/wt) PHB with a relative molecular mass of 1.9 MDa, close to that of PHB obtained using glucose. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
Fil:De Almeida, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
Appl. Environ. Microbiol. 2010;76(6):2036-2040
Materia
Agitation conditions
Batch culture
Bioreactor cultures
Poly(3-hydroxybutyrate)
Recombinant Escherichia coli
Relative molecular mass
Batch cell culture
Cell culture
Escherichia coli
Ethanol
Glycerol
Glucose
alcohol
carboxylic acid
glucose
glycerol
hydroxybutyric acid
poly(3 hydroxybutyric acid)
poly-beta-hydroxybutyrate
polyester
recombinant protein
aeration
alcohol
bioreactor
coliform bacterium
ester
glucose
recombination
aerobic metabolism
article
Azotobacter
bioreactor
chemistry
Escherichia coli
fermentation
genetics
metabolism
molecular weight
Aerobiosis
Azotobacter
Bioreactors
Carboxylic Acids
Escherichia coli
Ethanol
Fermentation
Glucose
Glycerol
Hydroxybutyrates
Molecular Weight
Polyesters
Recombinant Proteins
Azotobacter
Bacteria (microorganisms)
Escherichia coli
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_00992240_v76_n6_p2036_DeAlmeida
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oai_identifier_str paperaa:paper_00992240_v76_n6_p2036_DeAlmeida
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Effects of aeration on the synthesis of poly(3-hydroxybutyrate) from glycerol and glucose in recombinant Escherichia coliDe Almeida, A.Giordano, A.M.Nikel, P.I.Pettinari, M.J.Agitation conditionsBatch cultureBioreactor culturesPoly(3-hydroxybutyrate)Recombinant Escherichia coliRelative molecular massBatch cell cultureCell cultureEscherichia coliEthanolGlycerolGlucosealcoholcarboxylic acidglucoseglycerolhydroxybutyric acidpoly(3 hydroxybutyric acid)poly-beta-hydroxybutyratepolyesterrecombinant proteinaerationalcoholbioreactorcoliform bacteriumesterglucoserecombinationaerobic metabolismarticleAzotobacterbioreactorchemistryEscherichia colifermentationgeneticsmetabolismmolecular weightAerobiosisAzotobacterBioreactorsCarboxylic AcidsEscherichia coliEthanolFermentationGlucoseGlycerolHydroxybutyratesMolecular WeightPolyestersRecombinant ProteinsAzotobacterBacteria (microorganisms)Escherichia coliBioreactor cultures of Escherichia coli recombinants carrying phaBAC and phaP of Azotobacter sp. FA8 grown on glycerol under low-agitation conditions accumulated more poly(3-hydroxybutyrate) (PHB) and ethanol than at high agitation, while in glucose cultures, low agitation led to a decrease in PHB formation. Cells produced smaller amounts of acids from glycerol than from glucose. Glycerol batch cultures stirred at 125 rpm accumulated, in 24 h, 30.1% (wt/wt) PHB with a relative molecular mass of 1.9 MDa, close to that of PHB obtained using glucose. Copyright © 2010, American Society for Microbiology. All Rights Reserved.Fil:De Almeida, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2010info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00992240_v76_n6_p2036_DeAlmeidaAppl. Environ. Microbiol. 2010;76(6):2036-2040reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-04T09:48:44Zpaperaa:paper_00992240_v76_n6_p2036_DeAlmeidaInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-04 09:48:46.248Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Effects of aeration on the synthesis of poly(3-hydroxybutyrate) from glycerol and glucose in recombinant Escherichia coli
title Effects of aeration on the synthesis of poly(3-hydroxybutyrate) from glycerol and glucose in recombinant Escherichia coli
spellingShingle Effects of aeration on the synthesis of poly(3-hydroxybutyrate) from glycerol and glucose in recombinant Escherichia coli
De Almeida, A.
Agitation conditions
Batch culture
Bioreactor cultures
Poly(3-hydroxybutyrate)
Recombinant Escherichia coli
Relative molecular mass
Batch cell culture
Cell culture
Escherichia coli
Ethanol
Glycerol
Glucose
alcohol
carboxylic acid
glucose
glycerol
hydroxybutyric acid
poly(3 hydroxybutyric acid)
poly-beta-hydroxybutyrate
polyester
recombinant protein
aeration
alcohol
bioreactor
coliform bacterium
ester
glucose
recombination
aerobic metabolism
article
Azotobacter
bioreactor
chemistry
Escherichia coli
fermentation
genetics
metabolism
molecular weight
Aerobiosis
Azotobacter
Bioreactors
Carboxylic Acids
Escherichia coli
Ethanol
Fermentation
Glucose
Glycerol
Hydroxybutyrates
Molecular Weight
Polyesters
Recombinant Proteins
Azotobacter
Bacteria (microorganisms)
Escherichia coli
title_short Effects of aeration on the synthesis of poly(3-hydroxybutyrate) from glycerol and glucose in recombinant Escherichia coli
title_full Effects of aeration on the synthesis of poly(3-hydroxybutyrate) from glycerol and glucose in recombinant Escherichia coli
title_fullStr Effects of aeration on the synthesis of poly(3-hydroxybutyrate) from glycerol and glucose in recombinant Escherichia coli
title_full_unstemmed Effects of aeration on the synthesis of poly(3-hydroxybutyrate) from glycerol and glucose in recombinant Escherichia coli
title_sort Effects of aeration on the synthesis of poly(3-hydroxybutyrate) from glycerol and glucose in recombinant Escherichia coli
dc.creator.none.fl_str_mv De Almeida, A.
Giordano, A.M.
Nikel, P.I.
Pettinari, M.J.
author De Almeida, A.
author_facet De Almeida, A.
Giordano, A.M.
Nikel, P.I.
Pettinari, M.J.
author_role author
author2 Giordano, A.M.
Nikel, P.I.
Pettinari, M.J.
author2_role author
author
author
dc.subject.none.fl_str_mv Agitation conditions
Batch culture
Bioreactor cultures
Poly(3-hydroxybutyrate)
Recombinant Escherichia coli
Relative molecular mass
Batch cell culture
Cell culture
Escherichia coli
Ethanol
Glycerol
Glucose
alcohol
carboxylic acid
glucose
glycerol
hydroxybutyric acid
poly(3 hydroxybutyric acid)
poly-beta-hydroxybutyrate
polyester
recombinant protein
aeration
alcohol
bioreactor
coliform bacterium
ester
glucose
recombination
aerobic metabolism
article
Azotobacter
bioreactor
chemistry
Escherichia coli
fermentation
genetics
metabolism
molecular weight
Aerobiosis
Azotobacter
Bioreactors
Carboxylic Acids
Escherichia coli
Ethanol
Fermentation
Glucose
Glycerol
Hydroxybutyrates
Molecular Weight
Polyesters
Recombinant Proteins
Azotobacter
Bacteria (microorganisms)
Escherichia coli
topic Agitation conditions
Batch culture
Bioreactor cultures
Poly(3-hydroxybutyrate)
Recombinant Escherichia coli
Relative molecular mass
Batch cell culture
Cell culture
Escherichia coli
Ethanol
Glycerol
Glucose
alcohol
carboxylic acid
glucose
glycerol
hydroxybutyric acid
poly(3 hydroxybutyric acid)
poly-beta-hydroxybutyrate
polyester
recombinant protein
aeration
alcohol
bioreactor
coliform bacterium
ester
glucose
recombination
aerobic metabolism
article
Azotobacter
bioreactor
chemistry
Escherichia coli
fermentation
genetics
metabolism
molecular weight
Aerobiosis
Azotobacter
Bioreactors
Carboxylic Acids
Escherichia coli
Ethanol
Fermentation
Glucose
Glycerol
Hydroxybutyrates
Molecular Weight
Polyesters
Recombinant Proteins
Azotobacter
Bacteria (microorganisms)
Escherichia coli
dc.description.none.fl_txt_mv Bioreactor cultures of Escherichia coli recombinants carrying phaBAC and phaP of Azotobacter sp. FA8 grown on glycerol under low-agitation conditions accumulated more poly(3-hydroxybutyrate) (PHB) and ethanol than at high agitation, while in glucose cultures, low agitation led to a decrease in PHB formation. Cells produced smaller amounts of acids from glycerol than from glucose. Glycerol batch cultures stirred at 125 rpm accumulated, in 24 h, 30.1% (wt/wt) PHB with a relative molecular mass of 1.9 MDa, close to that of PHB obtained using glucose. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
Fil:De Almeida, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description Bioreactor cultures of Escherichia coli recombinants carrying phaBAC and phaP of Azotobacter sp. FA8 grown on glycerol under low-agitation conditions accumulated more poly(3-hydroxybutyrate) (PHB) and ethanol than at high agitation, while in glucose cultures, low agitation led to a decrease in PHB formation. Cells produced smaller amounts of acids from glycerol than from glucose. Glycerol batch cultures stirred at 125 rpm accumulated, in 24 h, 30.1% (wt/wt) PHB with a relative molecular mass of 1.9 MDa, close to that of PHB obtained using glucose. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_00992240_v76_n6_p2036_DeAlmeida
url http://hdl.handle.net/20.500.12110/paper_00992240_v76_n6_p2036_DeAlmeida
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Appl. Environ. Microbiol. 2010;76(6):2036-2040
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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score 12.623145

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