Cell Autonomy of HIF Effects in Drosophila: Tracheal Cells Sense Hypoxia and Induce Terminal Branch Sprouting

Autores
Centanin, L.; Dekanty, A.; Romero, N.; Irisarri, M.; Gorr, T.A.; Wappner, P.
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Drosophila tracheal terminal branches are plastic and have the capacity to sprout out projections toward oxygen-starved areas, in a process analogous to mammalian angiogenesis. This response involves the upregulation of FGF/Branchless in hypoxic tissues, which binds its receptor Breathless on tracheal cells. Here, we show that extra sprouting depends on the Hypoxia-Inducible Factor (HIF)-α homolog Sima and on the HIF-prolyl hydroxylase Fatiga that operates as an oxygen sensor. In mild hypoxia, Sima accumulates in tracheal cells, where it induces breathless, and this induction is sufficient to provoke tracheal extra sprouting. In nontracheal cells, Sima contributes to branchless induction, whereas overexpression of Sima fails to attract terminal branch outgrowth, suggesting that HIF-independent components are also required for full induction of the ligand. We propose that the autonomous response to hypoxia that occurs in tracheal cells enhances tracheal sensitivity to increasing Branchless levels, and that this mechanism is a cardinal step in hypoxia-dependent tracheal sprouting. © 2008 Elsevier Inc. All rights reserved.
Fil:Centanin, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Dekanty, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Romero, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Irisarri, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Wappner, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
Dev. Cell 2008;14(4):547-558
Materia
DEVBIO
cell protein
fibroblast growth factor receptor
hypoxia inducible factor alpha
ligand
procollagen proline 2 oxoglutarate 4 dioxygenase
protein Fatiga
transcription factor
transcription factor Branchless
transcription factor Breathless
transcription factor Sima
unclassified drug
angiogenesis
animal cell
article
cell hypoxia
controlled study
Drosophila
embryo
gene overexpression
nonhuman
organogenesis
oxygen sensing
phenotype
priority journal
protein expression
protein localization
tissue oxygenation
trachea
wild type
Animals
Anoxia
Body Patterning
DNA-Binding Proteins
Drosophila melanogaster
Drosophila Proteins
Fibroblast Growth Factors
Gene Expression Regulation, Developmental
Hypoxia-Inducible Factor 1, alpha Subunit
Larva
Oxygen
Phenotype
Procollagen-Proline Dioxygenase
Protein-Tyrosine Kinases
Receptors, Fibroblast Growth Factor
Mammalia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_15345807_v14_n4_p547_Centanin
Acceder
id BDUBAFCEN_e1126f4f7422ac9c155292bd93f9c088
oai_identifier_str paperaa:paper_15345807_v14_n4_p547_Centanin
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Cell Autonomy of HIF Effects in Drosophila: Tracheal Cells Sense Hypoxia and Induce Terminal Branch SproutingCentanin, L.Dekanty, A.Romero, N.Irisarri, M.Gorr, T.A.Wappner, P.DEVBIOcell proteinfibroblast growth factor receptorhypoxia inducible factor alphaligandprocollagen proline 2 oxoglutarate 4 dioxygenaseprotein Fatigatranscription factortranscription factor Branchlesstranscription factor Breathlesstranscription factor Simaunclassified drugangiogenesisanimal cellarticlecell hypoxiacontrolled studyDrosophilaembryogene overexpressionnonhumanorganogenesisoxygen sensingphenotypepriority journalprotein expressionprotein localizationtissue oxygenationtracheawild typeAnimalsAnoxiaBody PatterningDNA-Binding ProteinsDrosophila melanogasterDrosophila ProteinsFibroblast Growth FactorsGene Expression Regulation, DevelopmentalHypoxia-Inducible Factor 1, alpha SubunitLarvaOxygenPhenotypeProcollagen-Proline DioxygenaseProtein-Tyrosine KinasesReceptors, Fibroblast Growth FactorMammaliaDrosophila tracheal terminal branches are plastic and have the capacity to sprout out projections toward oxygen-starved areas, in a process analogous to mammalian angiogenesis. This response involves the upregulation of FGF/Branchless in hypoxic tissues, which binds its receptor Breathless on tracheal cells. Here, we show that extra sprouting depends on the Hypoxia-Inducible Factor (HIF)-α homolog Sima and on the HIF-prolyl hydroxylase Fatiga that operates as an oxygen sensor. In mild hypoxia, Sima accumulates in tracheal cells, where it induces breathless, and this induction is sufficient to provoke tracheal extra sprouting. In nontracheal cells, Sima contributes to branchless induction, whereas overexpression of Sima fails to attract terminal branch outgrowth, suggesting that HIF-independent components are also required for full induction of the ligand. We propose that the autonomous response to hypoxia that occurs in tracheal cells enhances tracheal sensitivity to increasing Branchless levels, and that this mechanism is a cardinal step in hypoxia-dependent tracheal sprouting. © 2008 Elsevier Inc. All rights reserved.Fil:Centanin, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Dekanty, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Romero, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Irisarri, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Wappner, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2008info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_15345807_v14_n4_p547_CentaninDev. Cell 2008;14(4):547-558reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-10-23T11:18:31Zpaperaa:paper_15345807_v14_n4_p547_CentaninInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-10-23 11:18:33.141Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Cell Autonomy of HIF Effects in Drosophila: Tracheal Cells Sense Hypoxia and Induce Terminal Branch Sprouting
title Cell Autonomy of HIF Effects in Drosophila: Tracheal Cells Sense Hypoxia and Induce Terminal Branch Sprouting
spellingShingle Cell Autonomy of HIF Effects in Drosophila: Tracheal Cells Sense Hypoxia and Induce Terminal Branch Sprouting
Centanin, L.
DEVBIO
cell protein
fibroblast growth factor receptor
hypoxia inducible factor alpha
ligand
procollagen proline 2 oxoglutarate 4 dioxygenase
protein Fatiga
transcription factor
transcription factor Branchless
transcription factor Breathless
transcription factor Sima
unclassified drug
angiogenesis
animal cell
article
cell hypoxia
controlled study
Drosophila
embryo
gene overexpression
nonhuman
organogenesis
oxygen sensing
phenotype
priority journal
protein expression
protein localization
tissue oxygenation
trachea
wild type
Animals
Anoxia
Body Patterning
DNA-Binding Proteins
Drosophila melanogaster
Drosophila Proteins
Fibroblast Growth Factors
Gene Expression Regulation, Developmental
Hypoxia-Inducible Factor 1, alpha Subunit
Larva
Oxygen
Phenotype
Procollagen-Proline Dioxygenase
Protein-Tyrosine Kinases
Receptors, Fibroblast Growth Factor
Mammalia
title_short Cell Autonomy of HIF Effects in Drosophila: Tracheal Cells Sense Hypoxia and Induce Terminal Branch Sprouting
title_full Cell Autonomy of HIF Effects in Drosophila: Tracheal Cells Sense Hypoxia and Induce Terminal Branch Sprouting
title_fullStr Cell Autonomy of HIF Effects in Drosophila: Tracheal Cells Sense Hypoxia and Induce Terminal Branch Sprouting
title_full_unstemmed Cell Autonomy of HIF Effects in Drosophila: Tracheal Cells Sense Hypoxia and Induce Terminal Branch Sprouting
title_sort Cell Autonomy of HIF Effects in Drosophila: Tracheal Cells Sense Hypoxia and Induce Terminal Branch Sprouting
dc.creator.none.fl_str_mv Centanin, L.
Dekanty, A.
Romero, N.
Irisarri, M.
Gorr, T.A.
Wappner, P.
author Centanin, L.
author_facet Centanin, L.
Dekanty, A.
Romero, N.
Irisarri, M.
Gorr, T.A.
Wappner, P.
author_role author
author2 Dekanty, A.
Romero, N.
Irisarri, M.
Gorr, T.A.
Wappner, P.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv DEVBIO
cell protein
fibroblast growth factor receptor
hypoxia inducible factor alpha
ligand
procollagen proline 2 oxoglutarate 4 dioxygenase
protein Fatiga
transcription factor
transcription factor Branchless
transcription factor Breathless
transcription factor Sima
unclassified drug
angiogenesis
animal cell
article
cell hypoxia
controlled study
Drosophila
embryo
gene overexpression
nonhuman
organogenesis
oxygen sensing
phenotype
priority journal
protein expression
protein localization
tissue oxygenation
trachea
wild type
Animals
Anoxia
Body Patterning
DNA-Binding Proteins
Drosophila melanogaster
Drosophila Proteins
Fibroblast Growth Factors
Gene Expression Regulation, Developmental
Hypoxia-Inducible Factor 1, alpha Subunit
Larva
Oxygen
Phenotype
Procollagen-Proline Dioxygenase
Protein-Tyrosine Kinases
Receptors, Fibroblast Growth Factor
Mammalia
topic DEVBIO
cell protein
fibroblast growth factor receptor
hypoxia inducible factor alpha
ligand
procollagen proline 2 oxoglutarate 4 dioxygenase
protein Fatiga
transcription factor
transcription factor Branchless
transcription factor Breathless
transcription factor Sima
unclassified drug
angiogenesis
animal cell
article
cell hypoxia
controlled study
Drosophila
embryo
gene overexpression
nonhuman
organogenesis
oxygen sensing
phenotype
priority journal
protein expression
protein localization
tissue oxygenation
trachea
wild type
Animals
Anoxia
Body Patterning
DNA-Binding Proteins
Drosophila melanogaster
Drosophila Proteins
Fibroblast Growth Factors
Gene Expression Regulation, Developmental
Hypoxia-Inducible Factor 1, alpha Subunit
Larva
Oxygen
Phenotype
Procollagen-Proline Dioxygenase
Protein-Tyrosine Kinases
Receptors, Fibroblast Growth Factor
Mammalia
dc.description.none.fl_txt_mv Drosophila tracheal terminal branches are plastic and have the capacity to sprout out projections toward oxygen-starved areas, in a process analogous to mammalian angiogenesis. This response involves the upregulation of FGF/Branchless in hypoxic tissues, which binds its receptor Breathless on tracheal cells. Here, we show that extra sprouting depends on the Hypoxia-Inducible Factor (HIF)-α homolog Sima and on the HIF-prolyl hydroxylase Fatiga that operates as an oxygen sensor. In mild hypoxia, Sima accumulates in tracheal cells, where it induces breathless, and this induction is sufficient to provoke tracheal extra sprouting. In nontracheal cells, Sima contributes to branchless induction, whereas overexpression of Sima fails to attract terminal branch outgrowth, suggesting that HIF-independent components are also required for full induction of the ligand. We propose that the autonomous response to hypoxia that occurs in tracheal cells enhances tracheal sensitivity to increasing Branchless levels, and that this mechanism is a cardinal step in hypoxia-dependent tracheal sprouting. © 2008 Elsevier Inc. All rights reserved.
Fil:Centanin, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Dekanty, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Romero, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Irisarri, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Wappner, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description Drosophila tracheal terminal branches are plastic and have the capacity to sprout out projections toward oxygen-starved areas, in a process analogous to mammalian angiogenesis. This response involves the upregulation of FGF/Branchless in hypoxic tissues, which binds its receptor Breathless on tracheal cells. Here, we show that extra sprouting depends on the Hypoxia-Inducible Factor (HIF)-α homolog Sima and on the HIF-prolyl hydroxylase Fatiga that operates as an oxygen sensor. In mild hypoxia, Sima accumulates in tracheal cells, where it induces breathless, and this induction is sufficient to provoke tracheal extra sprouting. In nontracheal cells, Sima contributes to branchless induction, whereas overexpression of Sima fails to attract terminal branch outgrowth, suggesting that HIF-independent components are also required for full induction of the ligand. We propose that the autonomous response to hypoxia that occurs in tracheal cells enhances tracheal sensitivity to increasing Branchless levels, and that this mechanism is a cardinal step in hypoxia-dependent tracheal sprouting. © 2008 Elsevier Inc. All rights reserved.
publishDate 2008
dc.date.none.fl_str_mv 2008
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_15345807_v14_n4_p547_Centanin
url http://hdl.handle.net/20.500.12110/paper_15345807_v14_n4_p547_Centanin
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Dev. Cell 2008;14(4):547-558
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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score 12.982451

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