The TIP60 complex is a conserved coactivator of HIF1A

Autores
Perez Perri, Joel Ignacio; Dengler, Verónica L.; Audetat, Audrey; Pandey, Ahwan; Bonner, Elyzabeth A.; Urh, Marjeta; Mendez, Jacqui; Daniels, Danette L.; Wappner, Pablo; Galbraith, Matthew D.; Espinosa, Joaquín M.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Hypoxia-inducible factors (HIFs) are critical regulators of the cellular response to hypoxia. Despite their established roles in normal physiology and numerous pathologies, the molecular mechanisms by which they control gene expression remain poorly understood. We report here a conserved role for the TIP60 complex as a HIF1 transcriptional cofactor in Drosophila and human cells. TIP60 (KAT5) is required for HIF1-dependent gene expression in fly cells and embryos and colorectal cancer cells. HIF1A interacts with and recruits TIP60 to chromatin. TIP60 is dispensable for HIF1A association with its target genes but is required for HIF1A-dependent chromatin modification and RNA polymerase II activation in hypoxia. In human cells, global analysis of HIF1A-dependent gene activity reveals that most HIF1A targets require either TIP60, the CDK8-Mediator complex, or both as coactivators for full expression in hypoxia. Thus, HIF1A employs functionally diverse cofactors to regulate different subsets of genes within its transcriptional program.
Fil: Perez Perri, Joel Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Dengler, Verónica L.. Howard Hughes Medical Institute; Estados Unidos
Fil: Audetat, Audrey. Howard Hughes Medical Institute; Estados Unidos
Fil: Pandey, Ahwan. Howard Hughes Medical Institute; Estados Unidos
Fil: Bonner, Elyzabeth A.. State University Of Colorado Boulder; Estados Unidos
Fil: Urh, Marjeta. Promega Corporation; Estados Unidos
Fil: Mendez, Jacqui. Promega Corporation; Estados Unidos
Fil: Daniels, Danette L.. Promega Corporation; Estados Unidos
Fil: Wappner, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Galbraith, Matthew D.. Howard Hughes Medical Institute; Estados Unidos
Fil: Espinosa, Joaquín M.. Howard Hughes Medical Institute; Estados Unidos
Materia
Hypoxia Inducible Factor
Acetyl transferase
Transcription
Histone
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/18218
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/18218
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The TIP60 complex is a conserved coactivator of HIF1APerez Perri, Joel IgnacioDengler, Verónica L.Audetat, AudreyPandey, AhwanBonner, Elyzabeth A.Urh, MarjetaMendez, JacquiDaniels, Danette L.Wappner, PabloGalbraith, Matthew D.Espinosa, Joaquín M.Hypoxia Inducible FactorAcetyl transferaseTranscriptionHistonehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Hypoxia-inducible factors (HIFs) are critical regulators of the cellular response to hypoxia. Despite their established roles in normal physiology and numerous pathologies, the molecular mechanisms by which they control gene expression remain poorly understood. We report here a conserved role for the TIP60 complex as a HIF1 transcriptional cofactor in Drosophila and human cells. TIP60 (KAT5) is required for HIF1-dependent gene expression in fly cells and embryos and colorectal cancer cells. HIF1A interacts with and recruits TIP60 to chromatin. TIP60 is dispensable for HIF1A association with its target genes but is required for HIF1A-dependent chromatin modification and RNA polymerase II activation in hypoxia. In human cells, global analysis of HIF1A-dependent gene activity reveals that most HIF1A targets require either TIP60, the CDK8-Mediator complex, or both as coactivators for full expression in hypoxia. Thus, HIF1A employs functionally diverse cofactors to regulate different subsets of genes within its transcriptional program.Fil: Perez Perri, Joel Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Dengler, Verónica L.. Howard Hughes Medical Institute; Estados UnidosFil: Audetat, Audrey. Howard Hughes Medical Institute; Estados UnidosFil: Pandey, Ahwan. Howard Hughes Medical Institute; Estados UnidosFil: Bonner, Elyzabeth A.. State University Of Colorado Boulder; Estados UnidosFil: Urh, Marjeta. Promega Corporation; Estados UnidosFil: Mendez, Jacqui. Promega Corporation; Estados UnidosFil: Daniels, Danette L.. Promega Corporation; Estados UnidosFil: Wappner, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Galbraith, Matthew D.. Howard Hughes Medical Institute; Estados UnidosFil: Espinosa, Joaquín M.. Howard Hughes Medical Institute; Estados UnidosElsevier2016-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/18218Perez Perri, Joel Ignacio; Dengler, Verónica L.; Audetat, Audrey; Pandey, Ahwan; Bonner, Elyzabeth A.; et al.; The TIP60 complex is a conserved coactivator of HIF1A; Elsevier; Cell Reports; 16; 1; 6-2016; 37-472211-1247CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S2211124716306957info:eu-repo/semantics/altIdentifier/doi/10.1016/j.celrep.2016.05.082info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:05:22Zoai:ri.conicet.gov.ar:11336/18218instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:05:23.143CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The TIP60 complex is a conserved coactivator of HIF1A
title The TIP60 complex is a conserved coactivator of HIF1A
spellingShingle The TIP60 complex is a conserved coactivator of HIF1A
Perez Perri, Joel Ignacio
Hypoxia Inducible Factor
Acetyl transferase
Transcription
Histone
title_short The TIP60 complex is a conserved coactivator of HIF1A
title_full The TIP60 complex is a conserved coactivator of HIF1A
title_fullStr The TIP60 complex is a conserved coactivator of HIF1A
title_full_unstemmed The TIP60 complex is a conserved coactivator of HIF1A
title_sort The TIP60 complex is a conserved coactivator of HIF1A
dc.creator.none.fl_str_mv Perez Perri, Joel Ignacio
Dengler, Verónica L.
Audetat, Audrey
Pandey, Ahwan
Bonner, Elyzabeth A.
Urh, Marjeta
Mendez, Jacqui
Daniels, Danette L.
Wappner, Pablo
Galbraith, Matthew D.
Espinosa, Joaquín M.
author Perez Perri, Joel Ignacio
author_facet Perez Perri, Joel Ignacio
Dengler, Verónica L.
Audetat, Audrey
Pandey, Ahwan
Bonner, Elyzabeth A.
Urh, Marjeta
Mendez, Jacqui
Daniels, Danette L.
Wappner, Pablo
Galbraith, Matthew D.
Espinosa, Joaquín M.
author_role author
author2 Dengler, Verónica L.
Audetat, Audrey
Pandey, Ahwan
Bonner, Elyzabeth A.
Urh, Marjeta
Mendez, Jacqui
Daniels, Danette L.
Wappner, Pablo
Galbraith, Matthew D.
Espinosa, Joaquín M.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Hypoxia Inducible Factor
Acetyl transferase
Transcription
Histone
topic Hypoxia Inducible Factor
Acetyl transferase
Transcription
Histone
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Hypoxia-inducible factors (HIFs) are critical regulators of the cellular response to hypoxia. Despite their established roles in normal physiology and numerous pathologies, the molecular mechanisms by which they control gene expression remain poorly understood. We report here a conserved role for the TIP60 complex as a HIF1 transcriptional cofactor in Drosophila and human cells. TIP60 (KAT5) is required for HIF1-dependent gene expression in fly cells and embryos and colorectal cancer cells. HIF1A interacts with and recruits TIP60 to chromatin. TIP60 is dispensable for HIF1A association with its target genes but is required for HIF1A-dependent chromatin modification and RNA polymerase II activation in hypoxia. In human cells, global analysis of HIF1A-dependent gene activity reveals that most HIF1A targets require either TIP60, the CDK8-Mediator complex, or both as coactivators for full expression in hypoxia. Thus, HIF1A employs functionally diverse cofactors to regulate different subsets of genes within its transcriptional program.
Fil: Perez Perri, Joel Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Dengler, Verónica L.. Howard Hughes Medical Institute; Estados Unidos
Fil: Audetat, Audrey. Howard Hughes Medical Institute; Estados Unidos
Fil: Pandey, Ahwan. Howard Hughes Medical Institute; Estados Unidos
Fil: Bonner, Elyzabeth A.. State University Of Colorado Boulder; Estados Unidos
Fil: Urh, Marjeta. Promega Corporation; Estados Unidos
Fil: Mendez, Jacqui. Promega Corporation; Estados Unidos
Fil: Daniels, Danette L.. Promega Corporation; Estados Unidos
Fil: Wappner, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Galbraith, Matthew D.. Howard Hughes Medical Institute; Estados Unidos
Fil: Espinosa, Joaquín M.. Howard Hughes Medical Institute; Estados Unidos
description Hypoxia-inducible factors (HIFs) are critical regulators of the cellular response to hypoxia. Despite their established roles in normal physiology and numerous pathologies, the molecular mechanisms by which they control gene expression remain poorly understood. We report here a conserved role for the TIP60 complex as a HIF1 transcriptional cofactor in Drosophila and human cells. TIP60 (KAT5) is required for HIF1-dependent gene expression in fly cells and embryos and colorectal cancer cells. HIF1A interacts with and recruits TIP60 to chromatin. TIP60 is dispensable for HIF1A association with its target genes but is required for HIF1A-dependent chromatin modification and RNA polymerase II activation in hypoxia. In human cells, global analysis of HIF1A-dependent gene activity reveals that most HIF1A targets require either TIP60, the CDK8-Mediator complex, or both as coactivators for full expression in hypoxia. Thus, HIF1A employs functionally diverse cofactors to regulate different subsets of genes within its transcriptional program.
publishDate 2016
dc.date.none.fl_str_mv 2016-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/18218
Perez Perri, Joel Ignacio; Dengler, Verónica L.; Audetat, Audrey; Pandey, Ahwan; Bonner, Elyzabeth A.; et al.; The TIP60 complex is a conserved coactivator of HIF1A; Elsevier; Cell Reports; 16; 1; 6-2016; 37-47
2211-1247
CONICET Digital
CONICET
url http://hdl.handle.net/11336/18218
identifier_str_mv Perez Perri, Joel Ignacio; Dengler, Verónica L.; Audetat, Audrey; Pandey, Ahwan; Bonner, Elyzabeth A.; et al.; The TIP60 complex is a conserved coactivator of HIF1A; Elsevier; Cell Reports; 16; 1; 6-2016; 37-47
2211-1247
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S2211124716306957
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.celrep.2016.05.082
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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