Gene-by-Temperature Interactions and Candidate Plasticity Genes for Morphological Traits in Drosophila melanogaster

Autores
Carreira, V.P.; Imberti, M.A.; Mensch, J.; Fanara, J.J.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Understanding the genetic architecture of any quantitative trait requires identifying the genes involved in its expression in different environmental conditions. This goal can be achieved by mutagenesis screens in genetically tractable model organisms such as Drosophila melanogaster. Temperature during ontogenesis is an important environmental factor affecting development and phenotypic variation in holometabolous insects. In spite of the importance of phenotypic plasticity and genotype by environment interaction (GEI) for fitness related traits, its genetic basis has remained elusive. In this context, we analyzed five different adult morphological traits (face width, head width, thorax length, wing size and wing shape) in 42 co-isogenic single P-element insertional lines of Drosophila melanogaster raised at 17°C and 25°C. Our analyses showed that all lines differed from the control for at least one trait in males or females at either temperature. However, no line showed those differences for all traits in both sexes and temperatures simultaneously. In this sense, the most pleiotropic candidate genes were CG34460, Lsd-2 and Spn. Our analyses also revealed extensive genetic variation for all the characters mostly indicated by strong GEIs. Further, our results indicate that GEIs were predominantly explained by changes in ranking order in all cases suggesting that a moderate number of genes are involved in the expression of each character at both temperatures. Most lines displayed a plastic response for at least one trait in either sex. In this regard, P-element insertions affecting plasticity of a large number of traits were associated to the candidate genes Btk29A, CG43340, Drak and jim. Further studies will help to elucidate the relevance of these genes on the morphogenesis of different body structures in natural populations of D. melanogaster. © 2013 Carreira et al.
Fil:Carreira, V.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Imberti, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Mensch, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Fanara, J.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
PLoS ONE 2013;8(7)
Materia
article
Btk29A gene
CG34430 gene
CG34460 gene
controlled study
Drak gene
Drosophila melanogaster
female
gene
gene function
gene insertion
genetic analysis
genetic association
genetic variability
genotype
genotype environment interaction
insect genetics
jim gene
Lsd 2 gene
male
morphogenesis
morphological trait
nonhuman
ontogeny
phenotypic plasticity
Spn gene
temperature
Analysis of Variance
Animals
Body Size
Drosophila melanogaster
Female
Gene-Environment Interaction
Genotype
Male
Morphogenesis
Mutagenesis
Mutation
Phenotype
Quantitative Trait, Heritable
Sex Factors
Temperature
Wing
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_19326203_v8_n7_p_Carreira

id BDUBAFCEN_cbe3fa28df40f89cee74b9d13b4129b6
oai_identifier_str paperaa:paper_19326203_v8_n7_p_Carreira
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Gene-by-Temperature Interactions and Candidate Plasticity Genes for Morphological Traits in Drosophila melanogasterCarreira, V.P.Imberti, M.A.Mensch, J.Fanara, J.J.articleBtk29A geneCG34430 geneCG34460 genecontrolled studyDrak geneDrosophila melanogasterfemalegenegene functiongene insertiongenetic analysisgenetic associationgenetic variabilitygenotypegenotype environment interactioninsect geneticsjim geneLsd 2 genemalemorphogenesismorphological traitnonhumanontogenyphenotypic plasticitySpn genetemperatureAnalysis of VarianceAnimalsBody SizeDrosophila melanogasterFemaleGene-Environment InteractionGenotypeMaleMorphogenesisMutagenesisMutationPhenotypeQuantitative Trait, HeritableSex FactorsTemperatureWingUnderstanding the genetic architecture of any quantitative trait requires identifying the genes involved in its expression in different environmental conditions. This goal can be achieved by mutagenesis screens in genetically tractable model organisms such as Drosophila melanogaster. Temperature during ontogenesis is an important environmental factor affecting development and phenotypic variation in holometabolous insects. In spite of the importance of phenotypic plasticity and genotype by environment interaction (GEI) for fitness related traits, its genetic basis has remained elusive. In this context, we analyzed five different adult morphological traits (face width, head width, thorax length, wing size and wing shape) in 42 co-isogenic single P-element insertional lines of Drosophila melanogaster raised at 17°C and 25°C. Our analyses showed that all lines differed from the control for at least one trait in males or females at either temperature. However, no line showed those differences for all traits in both sexes and temperatures simultaneously. In this sense, the most pleiotropic candidate genes were CG34460, Lsd-2 and Spn. Our analyses also revealed extensive genetic variation for all the characters mostly indicated by strong GEIs. Further, our results indicate that GEIs were predominantly explained by changes in ranking order in all cases suggesting that a moderate number of genes are involved in the expression of each character at both temperatures. Most lines displayed a plastic response for at least one trait in either sex. In this regard, P-element insertions affecting plasticity of a large number of traits were associated to the candidate genes Btk29A, CG43340, Drak and jim. Further studies will help to elucidate the relevance of these genes on the morphogenesis of different body structures in natural populations of D. melanogaster. © 2013 Carreira et al.Fil:Carreira, V.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Imberti, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Mensch, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Fanara, J.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_19326203_v8_n7_p_CarreiraPLoS ONE 2013;8(7)reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:42:54Zpaperaa:paper_19326203_v8_n7_p_CarreiraInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:42:56.084Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Gene-by-Temperature Interactions and Candidate Plasticity Genes for Morphological Traits in Drosophila melanogaster
title Gene-by-Temperature Interactions and Candidate Plasticity Genes for Morphological Traits in Drosophila melanogaster
spellingShingle Gene-by-Temperature Interactions and Candidate Plasticity Genes for Morphological Traits in Drosophila melanogaster
Carreira, V.P.
article
Btk29A gene
CG34430 gene
CG34460 gene
controlled study
Drak gene
Drosophila melanogaster
female
gene
gene function
gene insertion
genetic analysis
genetic association
genetic variability
genotype
genotype environment interaction
insect genetics
jim gene
Lsd 2 gene
male
morphogenesis
morphological trait
nonhuman
ontogeny
phenotypic plasticity
Spn gene
temperature
Analysis of Variance
Animals
Body Size
Drosophila melanogaster
Female
Gene-Environment Interaction
Genotype
Male
Morphogenesis
Mutagenesis
Mutation
Phenotype
Quantitative Trait, Heritable
Sex Factors
Temperature
Wing
title_short Gene-by-Temperature Interactions and Candidate Plasticity Genes for Morphological Traits in Drosophila melanogaster
title_full Gene-by-Temperature Interactions and Candidate Plasticity Genes for Morphological Traits in Drosophila melanogaster
title_fullStr Gene-by-Temperature Interactions and Candidate Plasticity Genes for Morphological Traits in Drosophila melanogaster
title_full_unstemmed Gene-by-Temperature Interactions and Candidate Plasticity Genes for Morphological Traits in Drosophila melanogaster
title_sort Gene-by-Temperature Interactions and Candidate Plasticity Genes for Morphological Traits in Drosophila melanogaster
dc.creator.none.fl_str_mv Carreira, V.P.
Imberti, M.A.
Mensch, J.
Fanara, J.J.
author Carreira, V.P.
author_facet Carreira, V.P.
Imberti, M.A.
Mensch, J.
Fanara, J.J.
author_role author
author2 Imberti, M.A.
Mensch, J.
Fanara, J.J.
author2_role author
author
author
dc.subject.none.fl_str_mv article
Btk29A gene
CG34430 gene
CG34460 gene
controlled study
Drak gene
Drosophila melanogaster
female
gene
gene function
gene insertion
genetic analysis
genetic association
genetic variability
genotype
genotype environment interaction
insect genetics
jim gene
Lsd 2 gene
male
morphogenesis
morphological trait
nonhuman
ontogeny
phenotypic plasticity
Spn gene
temperature
Analysis of Variance
Animals
Body Size
Drosophila melanogaster
Female
Gene-Environment Interaction
Genotype
Male
Morphogenesis
Mutagenesis
Mutation
Phenotype
Quantitative Trait, Heritable
Sex Factors
Temperature
Wing
topic article
Btk29A gene
CG34430 gene
CG34460 gene
controlled study
Drak gene
Drosophila melanogaster
female
gene
gene function
gene insertion
genetic analysis
genetic association
genetic variability
genotype
genotype environment interaction
insect genetics
jim gene
Lsd 2 gene
male
morphogenesis
morphological trait
nonhuman
ontogeny
phenotypic plasticity
Spn gene
temperature
Analysis of Variance
Animals
Body Size
Drosophila melanogaster
Female
Gene-Environment Interaction
Genotype
Male
Morphogenesis
Mutagenesis
Mutation
Phenotype
Quantitative Trait, Heritable
Sex Factors
Temperature
Wing
dc.description.none.fl_txt_mv Understanding the genetic architecture of any quantitative trait requires identifying the genes involved in its expression in different environmental conditions. This goal can be achieved by mutagenesis screens in genetically tractable model organisms such as Drosophila melanogaster. Temperature during ontogenesis is an important environmental factor affecting development and phenotypic variation in holometabolous insects. In spite of the importance of phenotypic plasticity and genotype by environment interaction (GEI) for fitness related traits, its genetic basis has remained elusive. In this context, we analyzed five different adult morphological traits (face width, head width, thorax length, wing size and wing shape) in 42 co-isogenic single P-element insertional lines of Drosophila melanogaster raised at 17°C and 25°C. Our analyses showed that all lines differed from the control for at least one trait in males or females at either temperature. However, no line showed those differences for all traits in both sexes and temperatures simultaneously. In this sense, the most pleiotropic candidate genes were CG34460, Lsd-2 and Spn. Our analyses also revealed extensive genetic variation for all the characters mostly indicated by strong GEIs. Further, our results indicate that GEIs were predominantly explained by changes in ranking order in all cases suggesting that a moderate number of genes are involved in the expression of each character at both temperatures. Most lines displayed a plastic response for at least one trait in either sex. In this regard, P-element insertions affecting plasticity of a large number of traits were associated to the candidate genes Btk29A, CG43340, Drak and jim. Further studies will help to elucidate the relevance of these genes on the morphogenesis of different body structures in natural populations of D. melanogaster. © 2013 Carreira et al.
Fil:Carreira, V.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Imberti, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Mensch, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Fanara, J.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description Understanding the genetic architecture of any quantitative trait requires identifying the genes involved in its expression in different environmental conditions. This goal can be achieved by mutagenesis screens in genetically tractable model organisms such as Drosophila melanogaster. Temperature during ontogenesis is an important environmental factor affecting development and phenotypic variation in holometabolous insects. In spite of the importance of phenotypic plasticity and genotype by environment interaction (GEI) for fitness related traits, its genetic basis has remained elusive. In this context, we analyzed five different adult morphological traits (face width, head width, thorax length, wing size and wing shape) in 42 co-isogenic single P-element insertional lines of Drosophila melanogaster raised at 17°C and 25°C. Our analyses showed that all lines differed from the control for at least one trait in males or females at either temperature. However, no line showed those differences for all traits in both sexes and temperatures simultaneously. In this sense, the most pleiotropic candidate genes were CG34460, Lsd-2 and Spn. Our analyses also revealed extensive genetic variation for all the characters mostly indicated by strong GEIs. Further, our results indicate that GEIs were predominantly explained by changes in ranking order in all cases suggesting that a moderate number of genes are involved in the expression of each character at both temperatures. Most lines displayed a plastic response for at least one trait in either sex. In this regard, P-element insertions affecting plasticity of a large number of traits were associated to the candidate genes Btk29A, CG43340, Drak and jim. Further studies will help to elucidate the relevance of these genes on the morphogenesis of different body structures in natural populations of D. melanogaster. © 2013 Carreira et al.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_19326203_v8_n7_p_Carreira
url http://hdl.handle.net/20.500.12110/paper_19326203_v8_n7_p_Carreira
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv PLoS ONE 2013;8(7)
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
_version_ 1844618735050031104
score 13.070432